ES2118719T3 - METHOD FOR PREPARING LYSOZYME DIMERS. - Google Patents
METHOD FOR PREPARING LYSOZYME DIMERS.Info
- Publication number
- ES2118719T3 ES2118719T3 ES90902033T ES90902033T ES2118719T3 ES 2118719 T3 ES2118719 T3 ES 2118719T3 ES 90902033 T ES90902033 T ES 90902033T ES 90902033 T ES90902033 T ES 90902033T ES 2118719 T3 ES2118719 T3 ES 2118719T3
- Authority
- ES
- Spain
- Prior art keywords
- lisocima
- lysocyme
- lysocima
- monomer
- dimerization
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2462—Lysozyme (3.2.1.17)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Public Health (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Wood Science & Technology (AREA)
- Pain & Pain Management (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Rheumatology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
SE FACILITA UN METODO PARA PREPARAR UN PRODUCTO DE DIMERO DE LISOCIMAALTAMENTE PURIFICADA QUE COMPRENDE DIMERIZACION DEL MONOMERO DE LISOCIMA CON UN REACTIVO DE ACOPLAMIENTO DE SUBERIMIDATO EN UNA SOLUCION TAMPON AJUSTADA A UN PH 10, DETENIENDO LA DIMERIZACION EN UN PUNTO DADO REDUCIENDO EL PH A 7 VIA LA ADICION DE HCI U OTRA SOLUCION ACIDA ADECUADA Y PURIFICANDO LA LISOCIMA DIMERICA MEDIANTE UNA SERIE DE PASOS DE ELUCION LLEVADOS A CABO UTILIZANDO CROMATOGRAFIA DE COLUMNA DE RESINA DE INTERCAMBIO DE IONES. LA LISOCIMA MONOMERICA QUE QUEDA NO DIMERIZADA MEDIANTE EL PRIMER PASO DE DIMERIZACION SE RECICLA EN EL PROCESO PARA INCREMENTAR EL RENDIMIENTO. EL PRESENTE SISTEMA ES VENTAJOSO EN TANTO EN CUANTO QUE SE PUEDEN PREPARAR GRANDES CANTIDADES DE DIMERO DE LISOCIMA PURIFICADA EFICAZMENTE Y DE FORMA RELATIVAMENTE BARATA, Y EL DIMERO DE LISOCIMA PRODUCIDO ASI ES UTIL EN EL TRATAMIENTO DE ENFERMEDADES VIRALES Y/O BACTERIANAS SIN CAUSAR LOS EFECTOS CITOTOXICOS ASOCIADOS AL MONOMERO DE LISOCIMA.A METHOD IS PROVIDED FOR PREPARING A HIGHLY PURIFIED LYSOCIMA DIMER PRODUCT WHICH INCLUDES LISOCIMA MONOMER DIMERIZATION WITH A SUBERIMIDATE COUPLING REAGENT FITTED BY A 7 DAMINED A PIPE DIMENSION AT A 10 THE ADDITION OF HCI OR ANOTHER APPROPRIATE ACID SOLUTION AND PURIFYING THE DIMERIC LYSOCYME THROUGH A SERIES OF ELUTION STEPS CARRIED OUT USING ION EXCHANGE RESIN COLUMN CHROMATOGRAPHY. THE MONOMERIC LYSOCYME THAT IS NOT DIMERIZED BY THE FIRST DIMERIZATION STEP IS RECYCLED IN THE PROCESS TO INCREASE PERFORMANCE. THE PRESENT SYSTEM IS ADVANTAGEOUS IN SO FAR AS LARGE AMOUNTS OF EFFICIENTLY PURIFIED LYSOCIMA AND RELATIVELY CHEAP, CAN BE PREPARED, AND THE DYMER OF LISOCIMA CAUSED BY SALES AND OUR DISEASES IS SO USEFUL CYTOTOXICS ASSOCIATED WITH THE LISOCIMA MONOMER.
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/US1990/000140 WO1991010731A1 (en) | 1990-01-08 | 1990-01-08 | Method of preparing lysozyme dimers |
| CA002073351A CA2073351C (en) | 1990-01-08 | 1990-01-08 | Lysozyme dimers and method of preparing them |
| HU9202250A HU214509B (en) | 1990-01-08 | 1990-01-08 | Method of preparing of purified lysozyme dimers |
| RO92-0931A RO114805B1 (en) | 1990-01-08 | 1990-01-08 | Purified lyzozyme dimer and process for preparing the same |
| SG1996007945A SG49871A1 (en) | 1990-01-08 | 1990-01-08 | Method of preparing lysozyme dimers |
| OA60242A OA09706A (en) | 1990-01-08 | 1992-07-07 | Method of preparing lysozyme dimers. |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2118719T3 true ES2118719T3 (en) | 1998-10-01 |
Family
ID=33136306
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES90902033T Expired - Lifetime ES2118719T3 (en) | 1990-01-08 | 1990-01-08 | METHOD FOR PREPARING LYSOZYME DIMERS. |
Country Status (21)
| Country | Link |
|---|---|
| EP (1) | EP0509984B1 (en) |
| JP (1) | JP2732514B2 (en) |
| KR (1) | KR0158216B1 (en) |
| AU (1) | AU654651B2 (en) |
| BG (1) | BG61040B1 (en) |
| BR (1) | BR9007971A (en) |
| CA (1) | CA2073351C (en) |
| DE (1) | DE69032375T2 (en) |
| DK (1) | DK0509984T3 (en) |
| ES (1) | ES2118719T3 (en) |
| FI (1) | FI101399B1 (en) |
| HK (1) | HK1007456A1 (en) |
| HU (1) | HU214509B (en) |
| LT (1) | LT3422B (en) |
| LV (1) | LV10119B (en) |
| MC (2) | MC2242A1 (en) |
| NO (1) | NO302484B1 (en) |
| OA (1) | OA09706A (en) |
| RO (1) | RO114805B1 (en) |
| SG (1) | SG49871A1 (en) |
| WO (1) | WO1991010731A1 (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL173978B1 (en) * | 1992-07-13 | 1998-05-29 | Nika Health Products Ltd | Method of obtaining a preparation capable to retard the synthesis of endogenous cytotoxic cytokinins |
| US6183742B1 (en) | 1992-07-13 | 2001-02-06 | Nika Health Products, Limited | Applications of lysozyme dimer |
| DZ1964A1 (en) * | 1995-01-13 | 2002-10-15 | Nika Health Products Ltd | New applications of a lysozyme dimer. |
| US6123937A (en) * | 1997-03-14 | 2000-09-26 | Nika Health Products, Limited | Applications of lysozyme dimer |
| US7348301B2 (en) * | 2006-02-16 | 2008-03-25 | Buckman Laboratories International, Inc. | Lysozyme-based method and composition to control the growth of microorganisms in aqueous systems |
| CA2851270C (en) * | 2011-10-05 | 2023-03-14 | The Rockefeller University | Dimeric bacteriophage lysins |
| CN111751468A (en) * | 2020-07-03 | 2020-10-09 | 上海艾魁英生物科技有限公司 | Method for purifying lysozyme dimer and preparing standard substance thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7850731B2 (en) | 2006-10-04 | 2010-12-14 | Seaspine, Inc. | Articulating spinal implant |
-
1990
- 1990-01-08 CA CA002073351A patent/CA2073351C/en not_active Expired - Lifetime
- 1990-01-08 KR KR1019920701566A patent/KR0158216B1/en not_active IP Right Cessation
- 1990-01-08 AU AU48474/90A patent/AU654651B2/en not_active Ceased
- 1990-01-08 MC MC90US9000140D patent/MC2242A1/en unknown
- 1990-01-08 JP JP2502143A patent/JP2732514B2/en not_active Expired - Fee Related
- 1990-01-08 DE DE69032375T patent/DE69032375T2/en not_active Expired - Fee Related
- 1990-01-08 ES ES90902033T patent/ES2118719T3/en not_active Expired - Lifetime
- 1990-01-08 MC MC90US9000140D patent/MC2243A1/en unknown
- 1990-01-08 EP EP90902033A patent/EP0509984B1/en not_active Expired - Lifetime
- 1990-01-08 DK DK90902033T patent/DK0509984T3/en active
- 1990-01-08 HU HU9202250A patent/HU214509B/en unknown
- 1990-01-08 WO PCT/US1990/000140 patent/WO1991010731A1/en active IP Right Grant
- 1990-01-08 BR BR909007971A patent/BR9007971A/en not_active Application Discontinuation
- 1990-01-08 RO RO92-0931A patent/RO114805B1/en unknown
- 1990-01-08 SG SG1996007945A patent/SG49871A1/en unknown
-
1992
- 1992-07-03 NO NO922626A patent/NO302484B1/en not_active IP Right Cessation
- 1992-07-07 BG BG96578A patent/BG61040B1/en unknown
- 1992-07-07 OA OA60242A patent/OA09706A/en unknown
- 1992-07-07 FI FI923123A patent/FI101399B1/en active
- 1992-12-28 LV LVP-92-506A patent/LV10119B/en unknown
-
1993
- 1993-06-02 LT LTIP594A patent/LT3422B/en not_active IP Right Cessation
-
1998
- 1998-06-25 HK HK98106623A patent/HK1007456A1/en not_active IP Right Cessation
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FC2A | Grant refused |
Effective date: 19920930 |
|
| FG2A | Definitive protection |
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