ES2118477T3 - Plasmido y celulas transfectadas con el mismo. - Google Patents

Plasmido y celulas transfectadas con el mismo.

Info

Publication number
ES2118477T3
ES2118477T3 ES95110058T ES95110058T ES2118477T3 ES 2118477 T3 ES2118477 T3 ES 2118477T3 ES 95110058 T ES95110058 T ES 95110058T ES 95110058 T ES95110058 T ES 95110058T ES 2118477 T3 ES2118477 T3 ES 2118477T3
Authority
ES
Spain
Prior art keywords
plasmid
same
cells transfected
objective
way
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95110058T
Other languages
English (en)
Inventor
Wolfgang Dr Hammerschmidt
Bill Prof Dr Sugden
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Helmholtz Zentrum Muenchen Deutsches Forschungszentrum fuer Gesundheit und Umwelt GmbH
Original Assignee
Helmholtz Zentrum Muenchen Deutsches Forschungszentrum fuer Gesundheit und Umwelt GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Helmholtz Zentrum Muenchen Deutsches Forschungszentrum fuer Gesundheit und Umwelt GmbH filed Critical Helmholtz Zentrum Muenchen Deutsches Forschungszentrum fuer Gesundheit und Umwelt GmbH
Application granted granted Critical
Publication of ES2118477T3 publication Critical patent/ES2118477T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16211—Lymphocryptovirus, e.g. human herpesvirus 4, Epstein-Barr Virus
    • C12N2710/16241—Use of virus, viral particle or viral elements as a vector
    • C12N2710/16243—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

LA INVENCION SE REFIERE A UNA PLASMIDA COMPUESTA DE PARTES DEL VIRUS (EBV) EPSTEIN-BARR Y PARTES DE VECTORES PROCARIONTICOS. EL OBJETIVO DE LA INVENCION ES LA CONSTITUCION DE LA PLASMIDA DE TAL MODO, QUE SE REALIZA LA GENERACION DE CELULAS INMORTALIZADAS LIBRES DE VIRUS. SE CONSIGUE ESTE OBJETIVO DE TAL MODO, QUE A PARTIR DE LOS GENES EBV ESTAN CONTENIDOS: LMP1 DESDE LA POSICION 169546 HASTA LA POSICION 16950, EBNA2 DESDE POSICION 48504 HASTA POSICION 49946, EBNA3A DESDE POSICION 92238 HASTA POSICION 95248, EBNA3C DESDE POSICION 98323 HASTA POSICION 101420 Y EBNA1 DESDE POSICION 107950 HASTA POSICION 109872.
ES95110058T 1994-07-30 1995-06-28 Plasmido y celulas transfectadas con el mismo. Expired - Lifetime ES2118477T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE4427117A DE4427117C1 (de) 1994-07-30 1994-07-30 Plasmid und damit transfizierte Zellen

Publications (1)

Publication Number Publication Date
ES2118477T3 true ES2118477T3 (es) 1998-09-16

Family

ID=6524590

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95110058T Expired - Lifetime ES2118477T3 (es) 1994-07-30 1995-06-28 Plasmido y celulas transfectadas con el mismo.

Country Status (3)

Country Link
EP (1) EP0694613B1 (es)
DE (2) DE4427117C1 (es)
ES (1) ES2118477T3 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0915165B1 (de) * 1997-11-05 2003-02-19 GSF-Forschungszentrum für Umwelt und Gesundheit GmbH Verfahren zur helfervirusfreien Verpackung einer Genvektor-DNA
ATE259421T1 (de) * 1997-11-05 2004-02-15 Gsf Forschungszentrum Umwelt Dna-virus-vektoren und verfahren zu ihrer herstellung
KR20200101394A (ko) * 2017-12-20 2020-08-27 글락소스미스클라인 바이오로지칼즈 에스.에이. 엡스타인-바 바이러스 항원 구축물

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5194601A (en) * 1989-09-18 1993-03-16 Wisconsin Alumni Research Foundation Lytic origin of replication for Epstein-Barr virus (EBV)

Also Published As

Publication number Publication date
EP0694613A1 (de) 1996-01-31
EP0694613B1 (de) 1998-05-13
DE59502153D1 (de) 1998-06-18
DE4427117C1 (de) 1995-10-19

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