ES2103195A1 - Procedimiento para la produccion de vesiculas de membrana plasmatica de hongos y levaduras con orientacion controlada. - Google Patents

Procedimiento para la produccion de vesiculas de membrana plasmatica de hongos y levaduras con orientacion controlada.

Info

Publication number
ES2103195A1
ES2103195A1 ES09501249A ES9501249A ES2103195A1 ES 2103195 A1 ES2103195 A1 ES 2103195A1 ES 09501249 A ES09501249 A ES 09501249A ES 9501249 A ES9501249 A ES 9501249A ES 2103195 A1 ES2103195 A1 ES 2103195A1
Authority
ES
Spain
Prior art keywords
plasma membrane
membrane vesicles
procedure
orientation
yeasts
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES09501249A
Other languages
English (en)
Other versions
ES2103195B1 (es
Inventor
Martinez Unai Ona Ugalde
Martinez M Amaya Menendez
Christer Larson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Euskal Herriko Unibertsitatea
Original Assignee
Euskal Herriko Unibertsitatea
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Euskal Herriko Unibertsitatea filed Critical Euskal Herriko Unibertsitatea
Priority to ES9501249A priority Critical patent/ES2103195B1/es
Publication of ES2103195A1 publication Critical patent/ES2103195A1/es
Application granted granted Critical
Publication of ES2103195B1 publication Critical patent/ES2103195B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)

Abstract

PROCEDIMIENTO PARA LA PRODUCCION DE VESICULAS DE MEMBRANA PLASMATICA DE HONGOS Y LEVADURAS CON ORIENTACION CONTROLADA. EL PROCEDIMIENTO COMPRENDE UNA COMBINACION DE LISIS OSMOTICA, CENTRIFUGACION DIFERENCIAL Y SEPARACION DE LAS VESICULAS DE MEMBRANA PLASMATICA CON ORIENTACION CONTROLADA EN UN SISTEMA ACUOSO DE DOS FASES POLIMERICAS CONSTITUIDO POR POLIETILENGLICOL Y DEXTRANO. LAS VESICULAS OBTENIDAS TIENEN UNA ORIENTACION DE MEMBRANA INVERTIDAS CON RESPECTO A LA ORIENTACION NORMAL DE LA CELARLA. LAS VESICULAS DE MEMBRANA PLASMATICA OBTENIDAS PUEDE SER EMPLEADA COMO REACTIVO SONDA EN PRUEBAS DE RASTREO BIOLOGICO IN VITRO PARA SELECCIONAR COMPUESTOS ACTIVOS TALES COMO ANTIFUNGICOS U OTRAS PRUEBAS SIMILARES.
ES9501249A 1995-06-22 1995-06-22 Procedimiento para la produccion de vesiculas de membrana plasmatica de hongos y levaduras con orientacion controlada. Expired - Fee Related ES2103195B1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9501249A ES2103195B1 (es) 1995-06-22 1995-06-22 Procedimiento para la produccion de vesiculas de membrana plasmatica de hongos y levaduras con orientacion controlada.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9501249A ES2103195B1 (es) 1995-06-22 1995-06-22 Procedimiento para la produccion de vesiculas de membrana plasmatica de hongos y levaduras con orientacion controlada.

Publications (2)

Publication Number Publication Date
ES2103195A1 true ES2103195A1 (es) 1997-08-16
ES2103195B1 ES2103195B1 (es) 1998-03-01

Family

ID=8290818

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9501249A Expired - Fee Related ES2103195B1 (es) 1995-06-22 1995-06-22 Procedimiento para la produccion de vesiculas de membrana plasmatica de hongos y levaduras con orientacion controlada.

Country Status (1)

Country Link
ES (1) ES2103195B1 (es)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002000778A1 (fr) * 2000-06-23 2002-01-03 Tuo Jin Preparation de suspension aqueuse stable macromoleculaire en phase liquide-liquide
WO2003025565A3 (en) * 2001-09-21 2003-05-30 Caprion Pharmaceuticals Inc Preparation of highly-purified plasma membranes

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989010566A1 (en) * 1988-04-22 1989-11-02 Massachusetts Institute Of Technology Process for forming and using microdroplets

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989010566A1 (en) * 1988-04-22 1989-11-02 Massachusetts Institute Of Technology Process for forming and using microdroplets

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
L.A. OKOROKOV et al. "Isolation of Plasma Membrane Vesicles From the Yeast Saccharomyces Carlsbergensis Suitable for Solute Transport Studies". Biol. Mem., Vol. 3 (6) 1990 pßginas 795-809 *
LOUIS J. TRAFICANTE et al. "Vesicle Penicillirase of Bacillus Licheniformis 749/C Apparent identity with the plasma membrane enzyme". Biochimica et Biophysica Acta 467 (1977) pßginas 44-50 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002000778A1 (fr) * 2000-06-23 2002-01-03 Tuo Jin Preparation de suspension aqueuse stable macromoleculaire en phase liquide-liquide
WO2003025565A3 (en) * 2001-09-21 2003-05-30 Caprion Pharmaceuticals Inc Preparation of highly-purified plasma membranes

Also Published As

Publication number Publication date
ES2103195B1 (es) 1998-03-01

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