ES2103195A1 - Procedimiento para la produccion de vesiculas de membrana plasmatica de hongos y levaduras con orientacion controlada. - Google Patents
Procedimiento para la produccion de vesiculas de membrana plasmatica de hongos y levaduras con orientacion controlada.Info
- Publication number
- ES2103195A1 ES2103195A1 ES09501249A ES9501249A ES2103195A1 ES 2103195 A1 ES2103195 A1 ES 2103195A1 ES 09501249 A ES09501249 A ES 09501249A ES 9501249 A ES9501249 A ES 9501249A ES 2103195 A1 ES2103195 A1 ES 2103195A1
- Authority
- ES
- Spain
- Prior art keywords
- plasma membrane
- membrane vesicles
- procedure
- orientation
- yeasts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
PROCEDIMIENTO PARA LA PRODUCCION DE VESICULAS DE MEMBRANA PLASMATICA DE HONGOS Y LEVADURAS CON ORIENTACION CONTROLADA. EL PROCEDIMIENTO COMPRENDE UNA COMBINACION DE LISIS OSMOTICA, CENTRIFUGACION DIFERENCIAL Y SEPARACION DE LAS VESICULAS DE MEMBRANA PLASMATICA CON ORIENTACION CONTROLADA EN UN SISTEMA ACUOSO DE DOS FASES POLIMERICAS CONSTITUIDO POR POLIETILENGLICOL Y DEXTRANO. LAS VESICULAS OBTENIDAS TIENEN UNA ORIENTACION DE MEMBRANA INVERTIDAS CON RESPECTO A LA ORIENTACION NORMAL DE LA CELARLA. LAS VESICULAS DE MEMBRANA PLASMATICA OBTENIDAS PUEDE SER EMPLEADA COMO REACTIVO SONDA EN PRUEBAS DE RASTREO BIOLOGICO IN VITRO PARA SELECCIONAR COMPUESTOS ACTIVOS TALES COMO ANTIFUNGICOS U OTRAS PRUEBAS SIMILARES.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9501249A ES2103195B1 (es) | 1995-06-22 | 1995-06-22 | Procedimiento para la produccion de vesiculas de membrana plasmatica de hongos y levaduras con orientacion controlada. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9501249A ES2103195B1 (es) | 1995-06-22 | 1995-06-22 | Procedimiento para la produccion de vesiculas de membrana plasmatica de hongos y levaduras con orientacion controlada. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2103195A1 true ES2103195A1 (es) | 1997-08-16 |
ES2103195B1 ES2103195B1 (es) | 1998-03-01 |
Family
ID=8290818
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES9501249A Expired - Fee Related ES2103195B1 (es) | 1995-06-22 | 1995-06-22 | Procedimiento para la produccion de vesiculas de membrana plasmatica de hongos y levaduras con orientacion controlada. |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES2103195B1 (es) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2002000778A1 (fr) * | 2000-06-23 | 2002-01-03 | Tuo Jin | Preparation de suspension aqueuse stable macromoleculaire en phase liquide-liquide |
WO2003025565A3 (en) * | 2001-09-21 | 2003-05-30 | Caprion Pharmaceuticals Inc | Preparation of highly-purified plasma membranes |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1989010566A1 (en) * | 1988-04-22 | 1989-11-02 | Massachusetts Institute Of Technology | Process for forming and using microdroplets |
-
1995
- 1995-06-22 ES ES9501249A patent/ES2103195B1/es not_active Expired - Fee Related
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1989010566A1 (en) * | 1988-04-22 | 1989-11-02 | Massachusetts Institute Of Technology | Process for forming and using microdroplets |
Non-Patent Citations (2)
Title |
---|
L.A. OKOROKOV et al. "Isolation of Plasma Membrane Vesicles From the Yeast Saccharomyces Carlsbergensis Suitable for Solute Transport Studies". Biol. Mem., Vol. 3 (6) 1990 pßginas 795-809 * |
LOUIS J. TRAFICANTE et al. "Vesicle Penicillirase of Bacillus Licheniformis 749/C Apparent identity with the plasma membrane enzyme". Biochimica et Biophysica Acta 467 (1977) pßginas 44-50 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2002000778A1 (fr) * | 2000-06-23 | 2002-01-03 | Tuo Jin | Preparation de suspension aqueuse stable macromoleculaire en phase liquide-liquide |
WO2003025565A3 (en) * | 2001-09-21 | 2003-05-30 | Caprion Pharmaceuticals Inc | Preparation of highly-purified plasma membranes |
Also Published As
Publication number | Publication date |
---|---|
ES2103195B1 (es) | 1998-03-01 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EC2A | Search report published |
Date of ref document: 19970816 Kind code of ref document: A1 Effective date: 19970816 |
|
FD2A | Announcement of lapse in spain |
Effective date: 20150929 |