ES2092441A1 - Process for detecting the presence of potato virus "Y" (PVY) - Google Patents

Process for detecting the presence of potato virus "Y" (PVY)

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Publication number
ES2092441A1
ES2092441A1 ES09402138A ES9402138A ES2092441A1 ES 2092441 A1 ES2092441 A1 ES 2092441A1 ES 09402138 A ES09402138 A ES 09402138A ES 9402138 A ES9402138 A ES 9402138A ES 2092441 A1 ES2092441 A1 ES 2092441A1
Authority
ES
Spain
Prior art keywords
pvy
strains
detecting
potato virus
amplification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES09402138A
Other languages
Spanish (es)
Other versions
ES2092441B1 (en
Inventor
Pascual Vicente Torres
Ascaso Fernando Ponz
Sanchez Florentina Sanchez
Urgoiti Begona Blanco
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
INVEST Y MEJORA AGRARIA GRANJA
Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria INIA
Original Assignee
INVEST Y MEJORA AGRARIA GRANJA
Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria INIA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by INVEST Y MEJORA AGRARIA GRANJA, Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria INIA filed Critical INVEST Y MEJORA AGRARIA GRANJA
Priority to ES09402138A priority Critical patent/ES2092441B1/en
Publication of ES2092441A1 publication Critical patent/ES2092441A1/en
Application granted granted Critical
Publication of ES2092441B1 publication Critical patent/ES2092441B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Process for detecting the presence of potato virus "Y" (PVY). The process comprises (a) the immobilization of macromolecular structures related to the pathogen on a solid substrate, (b) the synthesis of cDNAs, (c) enzyme amplification of the synthesized cDNAs using specific starters, (d) enzyme digestion of the products resulting from amplification with the restriction enzymes Hinfl, Ddel, EcoRV, Rsal and Taql, (e) fractionation of the products resulting from enzyme digestion and (f) analysis of the said fractions. This process has applications in agriculture as it provides useful information to determine the presence of strains of PVY which are regarded as quarantine strains which prohibit the marketing of products contaminated with these strains. Preferred figure 1.
ES09402138A 1994-10-13 1994-10-13 PROCEDURE TO DETECT THE PRESENCE OF VIRUS "AND" OF THE POTATO (PVY) Expired - Fee Related ES2092441B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES09402138A ES2092441B1 (en) 1994-10-13 1994-10-13 PROCEDURE TO DETECT THE PRESENCE OF VIRUS "AND" OF THE POTATO (PVY)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES09402138A ES2092441B1 (en) 1994-10-13 1994-10-13 PROCEDURE TO DETECT THE PRESENCE OF VIRUS "AND" OF THE POTATO (PVY)

Publications (2)

Publication Number Publication Date
ES2092441A1 true ES2092441A1 (en) 1996-11-16
ES2092441B1 ES2092441B1 (en) 1997-06-01

Family

ID=8287707

Family Applications (1)

Application Number Title Priority Date Filing Date
ES09402138A Expired - Fee Related ES2092441B1 (en) 1994-10-13 1994-10-13 PROCEDURE TO DETECT THE PRESENCE OF VIRUS "AND" OF THE POTATO (PVY)

Country Status (1)

Country Link
ES (1) ES2092441B1 (en)

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CANDRESSE, T. et al., Detection of plum pox potyvirus and analysis of its molecular variability using immunocapture-PCR, EPPO Bulletin, 1994, Vol. 24, pßgs. 585-594. * Ver todo el documento * *
JANSEN, R. W., et al., Molecular epidemiology of human hepatitis A virus defined by an antigen-capture polymerase chain reaction method, Proc. Natl. Acad. Sci. USA, 1990, Vol. 87, pßgs. 2867-2871. * Ver todo el documento * *
WETZEL, T. et al., A highly sensitive immunocapture polymerase chain reaction method for plum pox potyvirus detection, J. Virol. Methods, 1992, Vol. 39, pßgs. 27-37. * Ver todo el documento * *

Also Published As

Publication number Publication date
ES2092441B1 (en) 1997-06-01

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