ES2092441A1 - Process for detecting the presence of potato virus "Y" (PVY) - Google Patents
Process for detecting the presence of potato virus "Y" (PVY)Info
- Publication number
- ES2092441A1 ES2092441A1 ES09402138A ES9402138A ES2092441A1 ES 2092441 A1 ES2092441 A1 ES 2092441A1 ES 09402138 A ES09402138 A ES 09402138A ES 9402138 A ES9402138 A ES 9402138A ES 2092441 A1 ES2092441 A1 ES 2092441A1
- Authority
- ES
- Spain
- Prior art keywords
- pvy
- strains
- detecting
- potato virus
- amplification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Process for detecting the presence of potato virus "Y" (PVY). The process comprises (a) the immobilization of macromolecular structures related to the pathogen on a solid substrate, (b) the synthesis of cDNAs, (c) enzyme amplification of the synthesized cDNAs using specific starters, (d) enzyme digestion of the products resulting from amplification with the restriction enzymes Hinfl, Ddel, EcoRV, Rsal and Taql, (e) fractionation of the products resulting from enzyme digestion and (f) analysis of the said fractions. This process has applications in agriculture as it provides useful information to determine the presence of strains of PVY which are regarded as quarantine strains which prohibit the marketing of products contaminated with these strains. Preferred figure 1.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES09402138A ES2092441B1 (en) | 1994-10-13 | 1994-10-13 | PROCEDURE TO DETECT THE PRESENCE OF VIRUS "AND" OF THE POTATO (PVY) |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES09402138A ES2092441B1 (en) | 1994-10-13 | 1994-10-13 | PROCEDURE TO DETECT THE PRESENCE OF VIRUS "AND" OF THE POTATO (PVY) |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2092441A1 true ES2092441A1 (en) | 1996-11-16 |
ES2092441B1 ES2092441B1 (en) | 1997-06-01 |
Family
ID=8287707
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES09402138A Expired - Fee Related ES2092441B1 (en) | 1994-10-13 | 1994-10-13 | PROCEDURE TO DETECT THE PRESENCE OF VIRUS "AND" OF THE POTATO (PVY) |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES2092441B1 (en) |
-
1994
- 1994-10-13 ES ES09402138A patent/ES2092441B1/en not_active Expired - Fee Related
Non-Patent Citations (3)
Title |
---|
CANDRESSE, T. et al., Detection of plum pox potyvirus and analysis of its molecular variability using immunocapture-PCR, EPPO Bulletin, 1994, Vol. 24, pßgs. 585-594. * Ver todo el documento * * |
JANSEN, R. W., et al., Molecular epidemiology of human hepatitis A virus defined by an antigen-capture polymerase chain reaction method, Proc. Natl. Acad. Sci. USA, 1990, Vol. 87, pßgs. 2867-2871. * Ver todo el documento * * |
WETZEL, T. et al., A highly sensitive immunocapture polymerase chain reaction method for plum pox potyvirus detection, J. Virol. Methods, 1992, Vol. 39, pßgs. 27-37. * Ver todo el documento * * |
Also Published As
Publication number | Publication date |
---|---|
ES2092441B1 (en) | 1997-06-01 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EC2A | Search report published |
Date of ref document: 19961116 Kind code of ref document: A1 Effective date: 19961116 |
|
FD1A | Patent lapsed |
Effective date: 20100616 |