ES2091714A1 - Biosensor for the measurement of fructose, method for preparing it, and the application thereof for the analysis of fructose in food products. - Google Patents

Biosensor for the measurement of fructose, method for preparing it, and the application thereof for the analysis of fructose in food products.

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Publication number
ES2091714A1
ES2091714A1 ES09401507A ES9401507A ES2091714A1 ES 2091714 A1 ES2091714 A1 ES 2091714A1 ES 09401507 A ES09401507 A ES 09401507A ES 9401507 A ES9401507 A ES 9401507A ES 2091714 A1 ES2091714 A1 ES 2091714A1
Authority
ES
Spain
Prior art keywords
enzyme
fructose
mixture
biosensor
graphite
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES09401507A
Other languages
Spanish (es)
Other versions
ES2091714B1 (en
Inventor
Ferrer Josefina Parellada
Canas Elena Dominguez
Lopez Victor M Fernandez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
Universidad de Alcala de Henares UAH
Original Assignee
Consejo Superior de Investigaciones Cientificas CSIC
Universidad de Alcala de Henares UAH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Superior de Investigaciones Cientificas CSIC, Universidad de Alcala de Henares UAH filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Priority to ES9401507A priority Critical patent/ES2091714B1/en
Publication of ES2091714A1 publication Critical patent/ES2091714A1/en
Application granted granted Critical
Publication of ES2091714B1 publication Critical patent/ES2091714B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Biosensor for the measurement of fructose, method for preparing it, and the application thereof for the analysis of fructose in food products. Carbon paste biosensor for the measurement of fructose based on amperometric electrodes for direct electronic transfer between the enzyme fructose dehydrogenase, FDH, in which inactivation of the enzyme is prevented by paraffin oil, adding an aqueous solution of polyethylene imine, PEI, to the graphite-enzyme mixture, and, once the mixture is dry, adding the paraffin to form the electrode. The enzyme FDH can be the commercial enzyme isolated from Gluconosbacter sp. or from any other natural biological source or one obtained by genetic engineering techniques. Detergents are added to the mixture of the enzyme with the graphite. The graphite used is subjected to heat treatment (which can be with air) for more than 1 minute at a temperature greater than 650 degree C. The preparation of the biosensor is carried out in the following stages: a) A solution of enzyme in a pH 5 buffer containing a surfactant is used as the starting material b) The mixture is dried at reduced pressure at 3-8 degree C c) An aqueous solution of PEI buffered at pH 5 is added to it and the mixture is dried at 3-8 degree C d) The resultant graphite powder is mixed with paraffin oil, thus obtaining the corresponding modified carbon paste.
ES9401507A 1994-07-11 1994-07-11 BIOSENSOR FOR THE MEASUREMENT OF FRUCTOSE, WAY TO PREPARE IT AND ITS APPLICATION TO THE ANALYSIS OF FRUCTOSE IN FOOD PRODUCTS. Expired - Fee Related ES2091714B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9401507A ES2091714B1 (en) 1994-07-11 1994-07-11 BIOSENSOR FOR THE MEASUREMENT OF FRUCTOSE, WAY TO PREPARE IT AND ITS APPLICATION TO THE ANALYSIS OF FRUCTOSE IN FOOD PRODUCTS.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9401507A ES2091714B1 (en) 1994-07-11 1994-07-11 BIOSENSOR FOR THE MEASUREMENT OF FRUCTOSE, WAY TO PREPARE IT AND ITS APPLICATION TO THE ANALYSIS OF FRUCTOSE IN FOOD PRODUCTS.

Publications (2)

Publication Number Publication Date
ES2091714A1 true ES2091714A1 (en) 1996-11-01
ES2091714B1 ES2091714B1 (en) 1997-05-16

Family

ID=8286858

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9401507A Expired - Fee Related ES2091714B1 (en) 1994-07-11 1994-07-11 BIOSENSOR FOR THE MEASUREMENT OF FRUCTOSE, WAY TO PREPARE IT AND ITS APPLICATION TO THE ANALYSIS OF FRUCTOSE IN FOOD PRODUCTS.

Country Status (1)

Country Link
ES (1) ES2091714B1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005029069A1 (en) * 2003-08-29 2005-03-31 November Aktiengesellschaft Method for producing an electrode

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH03170856A (en) * 1989-11-30 1991-07-24 Nakano Vinegar Co Ltd Enzyme modified sensor
US5298144A (en) * 1992-09-15 1994-03-29 The Yellow Springs Instrument Company, Inc. Chemically wired fructose dehydrogenase electrodes
JPH0688804A (en) * 1992-03-13 1994-03-29 Matsushita Electric Ind Co Ltd Fructose sensor

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH03170856A (en) * 1989-11-30 1991-07-24 Nakano Vinegar Co Ltd Enzyme modified sensor
JPH0688804A (en) * 1992-03-13 1994-03-29 Matsushita Electric Ind Co Ltd Fructose sensor
US5298144A (en) * 1992-09-15 1994-03-29 The Yellow Springs Instrument Company, Inc. Chemically wired fructose dehydrogenase electrodes

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Base de datos WPIL en Questel, semana 9136, Londres: Derwent Publications Ltd., AN 91-262458 & JP 03170856 A (NAKANO SUMISE, KK) 24.07.91 * resumen * *
Base de datos WPIL en Questel, semana 9417, Londres: Derwent Publications Ltd., AN 94-141360 & JP 06088804 A (MATSUSHITA DENKI SANGYO, KK) 29.03.94 * resumen * *
Ikeda, T. et al., "Amperometric fructose sensor based on direct bioelectrocatalysis", 1991, Biosensors & Bioelectronics, vol. 6, pp.: 299-304. * todo el documento * *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005029069A1 (en) * 2003-08-29 2005-03-31 November Aktiengesellschaft Method for producing an electrode

Also Published As

Publication number Publication date
ES2091714B1 (en) 1997-05-16

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