ES2089970B1 - ISOLATION METHOD OF LACTOFERRIN AND OTHER DAIRY PROTEINS. - Google Patents
ISOLATION METHOD OF LACTOFERRIN AND OTHER DAIRY PROTEINS.Info
- Publication number
- ES2089970B1 ES2089970B1 ES9400477A ES9400477A ES2089970B1 ES 2089970 B1 ES2089970 B1 ES 2089970B1 ES 9400477 A ES9400477 A ES 9400477A ES 9400477 A ES9400477 A ES 9400477A ES 2089970 B1 ES2089970 B1 ES 2089970B1
- Authority
- ES
- Spain
- Prior art keywords
- lactoferrin
- isolation
- cellulose
- isolation method
- dairy proteins
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Peptides Or Proteins (AREA)
Abstract
EL OBJETO DE LA INVENCION ES UN METODO DE AISLAMIENTO DE LA LACTOFERRINA BASADO EN SU ABSORCION ESPECIFICA MEDIANTE UN LIGANDO INSOLUBILIZADO EN PLACAS DE CARTON DE CELULOSA FILTRANTE. EL AISLAMIENTO DE LACTOFERRINA SE HACE A PARTIR DE LACTOSUERO PROCEDENTE DE QUESERIA. ESTE SE HACE PASAR EN FLUJO CONTINUO POR UN DISPOSITIVO EN EL QUE SE ENCUENTRAN LAS PLACAS DE CELULOSA MODIFICADAS CON AZUL REACTIVO2, QUE RETIENEN FUNDAMENTALMENTE LA LACTOFERRINA. UNA VEZ LAVADA LA CELULOSA PARA ELIMINAR LAS PROTEINAS NO RETENIDAS, SE ELUYEN LAS RETENIDAS CON UNA SOLUCION SALINA. FINALMENTE, LA LACTOFERRINA OBTENIDA SE DIALIZA PARA ELIMINAR PARTE DE LA SAL Y SE LIOFILIZA, TRAS LO CUAL PUEDE SER UTILIZADA PARA SU ADICION A PRODUCTOS PARA USO ANIMAL O HUMANO.THE OBJECT OF THE INVENTION IS A METHOD OF ISOLATION OF LACTOFERRIN BASED ON ITS SPECIFIC ABSORPTION THROUGH A LIGAND INSOLUBILIZED IN FILTER CELLULOSE CARDBOARD PLATES. THE ISOLATION OF LACTOFERRIN IS MADE FROM LACTOSUERO FROM CHEESE. THIS IS PASSED IN A CONTINUOUS FLOW THROUGH A DEVICE IN WHICH THE REAGENT BLUE MODIFIED CELLULOSE PLATES ARE FOUND, WHICH FUNDAMENTALLY RETAIN THE LACTOFERRIN. ONCE THE CELLULOSE IS WASHED TO ELIMINATE NON-RETAINED PROTEINS, RETAINED PROTEINS ARE ELUTIOUS WITH A SALT SOLUTION. FINALLY, THE LACTOFERRIN OBTAINED IS DIALYZED TO ELIMINATE PART OF THE SALT AND IS LYOPHILIZED, AFTER WHICH IT CAN BE USED FOR ITS ADDITION TO PRODUCTS FOR ANIMAL OR HUMAN USE.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9400477A ES2089970B1 (en) | 1994-03-08 | 1994-03-08 | ISOLATION METHOD OF LACTOFERRIN AND OTHER DAIRY PROTEINS. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9400477A ES2089970B1 (en) | 1994-03-08 | 1994-03-08 | ISOLATION METHOD OF LACTOFERRIN AND OTHER DAIRY PROTEINS. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2089970A1 ES2089970A1 (en) | 1996-10-01 |
ES2089970B1 true ES2089970B1 (en) | 1997-05-01 |
Family
ID=8285479
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES9400477A Expired - Fee Related ES2089970B1 (en) | 1994-03-08 | 1994-03-08 | ISOLATION METHOD OF LACTOFERRIN AND OTHER DAIRY PROTEINS. |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES2089970B1 (en) |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2613368B1 (en) * | 1987-03-10 | 1989-06-23 | Entremont Sa | PROCESS FOR EXTRACTING WHEY PROTEINS BY ADSORPTION AND ELUTION |
ES2089797T3 (en) * | 1992-01-15 | 1996-10-01 | Campina Melkunie Bv | PROCEDURE TO ISOLATE LACTOFERRIN AND LACTOPEROXIDASE FROM MILK AND DAIRY PRODUCTS. |
-
1994
- 1994-03-08 ES ES9400477A patent/ES2089970B1/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
ES2089970A1 (en) | 1996-10-01 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FD2A | Announcement of lapse in spain |
Effective date: 20141120 |