ES2078185B1 - PROCEDURE FOR MOLECULAR TYPIFICATION OF VIRAL PATHOGENS WITH AN RNA GENOME AND OF SUBVIRAL PATHOGENS THROUGH RANDOM CDNA SYNTHESIS. - Google Patents

PROCEDURE FOR MOLECULAR TYPIFICATION OF VIRAL PATHOGENS WITH AN RNA GENOME AND OF SUBVIRAL PATHOGENS THROUGH RANDOM CDNA SYNTHESIS.

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Publication number
ES2078185B1
ES2078185B1 ES09400961A ES9400961A ES2078185B1 ES 2078185 B1 ES2078185 B1 ES 2078185B1 ES 09400961 A ES09400961 A ES 09400961A ES 9400961 A ES9400961 A ES 9400961A ES 2078185 B1 ES2078185 B1 ES 2078185B1
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ES
Spain
Prior art keywords
pathogens
procedure
typification
molecular
subviral
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES09400961A
Other languages
Spanish (es)
Other versions
ES2078185A1 (en
Inventor
Nolasco Gustavo Barbosa
Blas Beorlegui Carmen De
Herrera David Martinez
Sanchez Florentina Sanchez
Cano Javier Romero
Ascaso Fernando Ponz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria INIA
Original Assignee
Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria INIA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria INIA filed Critical Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria INIA
Priority to ES09400961A priority Critical patent/ES2078185B1/en
Publication of ES2078185A1 publication Critical patent/ES2078185A1/en
Application granted granted Critical
Publication of ES2078185B1 publication Critical patent/ES2078185B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PROCEDIMIENTO PARA LA TIPIFICACION MOLECULAR DE PATOGENOS VIRALES CON GENOMA RNA Y DE PATOGENOS SUBVIRALES MEDIANTE SINTESIS ALEATORIA DE CDNA. EL PROCEDIMIENTO COMPRENDE (A) LA INMOVILIZACION SOBRE UN SOPORTE SOLIDO DE ESTRUCTURAS MACROMOLECULARES RELACIONADAS CON EL PATOGENO, (B) LA SINTESIS ALEATORIA DE CDNA, PARA LO QUE SE SE EMPLEA UN CEBADOR INESPECIFICO EN LA REACCION DE SINTESIS DE LA PRIMERA CADENA DE TAJES DNAS COMPLEMENTARIOS, (C) LA AMPLIFICACION ENZIMATICA DE LOS CDNAS SINTETIZADOS EMPLEANDO CEBADORES ESPECIFICOS DE LA REGION DEL GENOMA A TIPIFICAR, (D) EL FRACCIONAMIENTO DE LOS PRODUCTOS RESULTANTES DE LA AMPLIFICACION Y (E) EL ANALISIS DE DICHO FRACCIONAMIENTO.ESTE PROCEDIMIENTO PROPORCIONA INFORMACION UTIL PARA EL ESTUDIO DE LA EPIDEMIOLOGIA Y EL DIAGNOSTICO DE PATOGENOS, ASI COMO EN PROGRAMAS DE BUSQUEDA DE GENES QUE CONFIERAN RESISTENCIA A DICHOS PATOGENOS Y EN PROYECTOS DE DISEÑO DE EPITOPOS PARA LA OBTENCION DE NUEVAS VACUNAS. ESTE PROCEDIMIENTO TIENE APLICACION, ESPECIALMENTE, EN LA AGRICULTURA.PROCEDURE FOR MOLECULAR TYPIFICATION OF VIRAL PATHOGENS WITH AN RNA GENOME AND OF SUBVIRAL PATHOGENS THROUGH RANDOM CDNA SYNTHESIS. THE PROCEDURE INCLUDES (A) IMMOBILIZATION ON A SOLID SUPPORT OF MACROMOLECULAR STRUCTURES RELATED TO THE PATHOGEN, (B) THE RANDOM SYNTHESIS OF CDNA, FOR WHICH AN INESPECIFIC PRIMER IS USED IN THE SYNTHESIS REACTION OF THE FIRST SUPPLEMENTARY, (C) THE ENZYMATIC AMPLIFICATION OF THE SYNTHESIZED CDNAS USING SPECIFIC PRIMERS OF THE REGION OF THE GENOME TO TYPE, (D) THE FRACTIONATION OF THE PRODUCTS RESULTING FROM THE AMPLIFICATION AND (E) PROPORTIONAL USE. FOR THE STUDY OF THE EPIDEMIOLOGY AND DIAGNOSIS OF PATHOGENS, AS WELL AS IN GENE SEARCH PROGRAMS THAT CONFER RESISTANCE TO SUCH PATHOGENS AND IN PROJECTS OF DESIGN OF EPITOPES FOR THE OBTAINING OF NEW VACCINES. THIS PROCEDURE HAS APPLICATION, ESPECIALLY, IN AGRICULTURE.

ES09400961A 1994-05-06 1994-05-06 PROCEDURE FOR MOLECULAR TYPIFICATION OF VIRAL PATHOGENS WITH AN RNA GENOME AND OF SUBVIRAL PATHOGENS THROUGH RANDOM CDNA SYNTHESIS. Expired - Fee Related ES2078185B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES09400961A ES2078185B1 (en) 1994-05-06 1994-05-06 PROCEDURE FOR MOLECULAR TYPIFICATION OF VIRAL PATHOGENS WITH AN RNA GENOME AND OF SUBVIRAL PATHOGENS THROUGH RANDOM CDNA SYNTHESIS.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES09400961A ES2078185B1 (en) 1994-05-06 1994-05-06 PROCEDURE FOR MOLECULAR TYPIFICATION OF VIRAL PATHOGENS WITH AN RNA GENOME AND OF SUBVIRAL PATHOGENS THROUGH RANDOM CDNA SYNTHESIS.

Publications (2)

Publication Number Publication Date
ES2078185A1 ES2078185A1 (en) 1995-12-01
ES2078185B1 true ES2078185B1 (en) 1996-08-16

Family

ID=8286130

Family Applications (1)

Application Number Title Priority Date Filing Date
ES09400961A Expired - Fee Related ES2078185B1 (en) 1994-05-06 1994-05-06 PROCEDURE FOR MOLECULAR TYPIFICATION OF VIRAL PATHOGENS WITH AN RNA GENOME AND OF SUBVIRAL PATHOGENS THROUGH RANDOM CDNA SYNTHESIS.

Country Status (1)

Country Link
ES (1) ES2078185B1 (en)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5077192A (en) * 1988-10-25 1991-12-31 The General Hospital Corporation Method of detecting antigenic, nucleic acid-containing macromolecular entities
IL92474A (en) * 1988-11-29 1994-04-12 Orion Yhtymae Oy Method and reagent combination for determining nucleotide sequences
GB2252323B (en) * 1991-02-01 1995-02-15 Secr Defence Virus detection method and oligonucleotides for use therein
ES2044784B1 (en) * 1992-06-12 1994-09-01 Inia PROCEDURE FOR THE DETECTION AND IDENTIFICATION OF VIRAL AND SUBVIRAL PATHOGENS.

Also Published As

Publication number Publication date
ES2078185A1 (en) 1995-12-01

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