ES2071567B1 - Metodo de valoracion toxicologica de un medio acuatico. - Google Patents

Metodo de valoracion toxicologica de un medio acuatico.

Info

Publication number
ES2071567B1
ES2071567B1 ES9301098A ES9301098A ES2071567B1 ES 2071567 B1 ES2071567 B1 ES 2071567B1 ES 9301098 A ES9301098 A ES 9301098A ES 9301098 A ES9301098 A ES 9301098A ES 2071567 B1 ES2071567 B1 ES 2071567B1
Authority
ES
Spain
Prior art keywords
toxicity
cell
expressed
measure
concentration
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES9301098A
Other languages
English (en)
Other versions
ES2071567A1 (es
Inventor
O Calvo Maria Argelia Casta
Lafarga Jose Vicente Tarazona
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria INIA
Original Assignee
Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria INIA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria INIA filed Critical Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria INIA
Priority to ES9301098A priority Critical patent/ES2071567B1/es
Publication of ES2071567A1 publication Critical patent/ES2071567A1/es
Application granted granted Critical
Publication of ES2071567B1 publication Critical patent/ES2071567B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

METODO DE VALORACION TOXICOLOGICA DE UN MEDIO ACUATICO. CONSISTE EN UN TEST {CVAIN VITRO QUE UTILIZA LA LINEA CELULAR ESTABLECIDA RTG-2, DERIVADA DE GONADA DE TRUCHA ARCOIRIS. EL TEST SE REALIZA MIDIENDO TRES PARAMETROS DE TOXICIDAD DIFERENTES SOBRE UNA MISMA POBLACION CELULAR, CONCRETAMENTE EL CONTENIDO INTRACELULAR DE ATP COMO MEDIDA DEL ESTADO ENERGETICO DE LAS CELULAS Y LA INTEGRIDAD DE LAS MEMBRANAS, LA INCORPORACION DEL COLORANTE SUPRAVITAL ROJO NEUTRO, COMO MEDIDA DE LA VIABILIDAD CELULAR, Y LA CANTIDAD TOTAL DE PROTEINA, COMO MEDIDA DE LA MORTALIDAD CELULAR. LA TOXICIDAD DE LAS SUSTANCIAS PURAS SE EXPRESA MEDIANTE LAS TRES CONCENTRACIONES EFECTIVAS MEDIAS (CE 50) QUE REFLEJAN LA CONCENTRACION DE SUSTANCIA NECESARIA, PARA OBSERVAR UNA REDUCCION A LA MITAD, DE LOS VALORES OBTENIDOS EN LA POBLACION CONTROL DE CELULAS NO EXPUESTAS. LA TOXICIDAD DE LAS MUESTRAS DE CAMPO, SE EXPRESA COMO PORCENTAJES FRENTE AL CONTROL DE CELULAS NO EXPUESTAS, DE CADA UNO DE LOS TRES PARAMETROS ESTUDIADOS,TENIENDO EN CUENTA LOS FACTORES DE CONCENTRACION/DILUCION APLICADOS A LA MUESTRA.
ES9301098A 1993-05-21 1993-05-21 Metodo de valoracion toxicologica de un medio acuatico. Expired - Fee Related ES2071567B1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9301098A ES2071567B1 (es) 1993-05-21 1993-05-21 Metodo de valoracion toxicologica de un medio acuatico.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9301098A ES2071567B1 (es) 1993-05-21 1993-05-21 Metodo de valoracion toxicologica de un medio acuatico.

Publications (2)

Publication Number Publication Date
ES2071567A1 ES2071567A1 (es) 1995-06-16
ES2071567B1 true ES2071567B1 (es) 1996-01-16

Family

ID=8281902

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9301098A Expired - Fee Related ES2071567B1 (es) 1993-05-21 1993-05-21 Metodo de valoracion toxicologica de un medio acuatico.

Country Status (1)

Country Link
ES (1) ES2071567B1 (es)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4023933A (en) * 1976-06-10 1977-05-17 The University Of Georgia Protein-assay reagent and method
SE8703675L (sv) * 1987-09-23 1989-03-24 Life Science International Ab Luminometrisk analys paa cellulaert atp
WO1990000619A1 (en) * 1988-07-08 1990-01-25 University College London Analysis of cell modifying substances
EP0585367A4 (en) * 1991-05-17 1994-12-21 Park Pharmaceuticals Inc ATP EXTRACTION REAGENT CONTAINING AMMONIUM VANADATE.

Also Published As

Publication number Publication date
ES2071567A1 (es) 1995-06-16

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Effective date: 20100315