ES2044778A1 - Analytical method for determining the fecundating capability of a human semen sample. - Google Patents
Analytical method for determining the fecundating capability of a human semen sample.Info
- Publication number
- ES2044778A1 ES2044778A1 ES9200700A ES9200700A ES2044778A1 ES 2044778 A1 ES2044778 A1 ES 2044778A1 ES 9200700 A ES9200700 A ES 9200700A ES 9200700 A ES9200700 A ES 9200700A ES 2044778 A1 ES2044778 A1 ES 2044778A1
- Authority
- ES
- Spain
- Prior art keywords
- semen sample
- fecundating
- capability
- determining
- analytical method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Analytical method for determining the fecundating capability of a human semen sample. Includes: (a) taking a drop of a solution comprising 0.5%-15% weight hyaluronic acid, 20%-90% weight calcium chloride and 0.1%-5% weight Triton X, spread on a slide and let dry (b) take a previously washed and capacitated semen sample and spread it on the slide previously covered with the solution, cover with a slide cover and leave to incubate for approximately 30 minutes to 48 hours at 25-40 degrees C (c) read the results under a phase contrast microscope, evaluating the halo formed and the size of its diameter. Application in assisted reproduction techniques to forecast the fecundating capability of a human semen sample.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9200700A ES2044778B1 (en) | 1992-04-02 | 1992-04-02 | ANALYTICAL METHOD FOR THE DETERMINATION OF THE FECUNDANT CAPACITY OF A SAMPLE OF HUMAN SEMEN. |
ES9401341A ES2094090B1 (en) | 1992-04-02 | 1994-06-20 | ANALYTICAL METHOD FOR THE DETERMINATION OF THE FECUNDANT CAPACITY OF A SAMPLE OF HUMAN SEMEN. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9200700A ES2044778B1 (en) | 1992-04-02 | 1992-04-02 | ANALYTICAL METHOD FOR THE DETERMINATION OF THE FECUNDANT CAPACITY OF A SAMPLE OF HUMAN SEMEN. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2044778A1 true ES2044778A1 (en) | 1994-01-01 |
ES2044778B1 ES2044778B1 (en) | 1994-09-01 |
Family
ID=8276593
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES9200700A Expired - Fee Related ES2044778B1 (en) | 1992-04-02 | 1992-04-02 | ANALYTICAL METHOD FOR THE DETERMINATION OF THE FECUNDANT CAPACITY OF A SAMPLE OF HUMAN SEMEN. |
ES9401341A Expired - Fee Related ES2094090B1 (en) | 1992-04-02 | 1994-06-20 | ANALYTICAL METHOD FOR THE DETERMINATION OF THE FECUNDANT CAPACITY OF A SAMPLE OF HUMAN SEMEN. |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES9401341A Expired - Fee Related ES2094090B1 (en) | 1992-04-02 | 1994-06-20 | ANALYTICAL METHOD FOR THE DETERMINATION OF THE FECUNDANT CAPACITY OF A SAMPLE OF HUMAN SEMEN. |
Country Status (1)
Country | Link |
---|---|
ES (2) | ES2044778B1 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2721405A1 (en) * | 1994-06-20 | 1995-12-22 | Esteve Carmen Blanco | Testing for sperm fecundity |
ES2387903A1 (en) * | 2010-07-16 | 2012-10-03 | María Albiñana Blanco | Procedure and kit to verify, in a sample of human or animal semen, the existence of fertilizing spermatozoids. (Machine-translation by Google Translate, not legally binding) |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0302765A1 (en) * | 1987-07-27 | 1989-02-08 | Clonatec | Method for detecting living spermatozoa, and tests therefor |
ES2006560A6 (en) * | 1988-01-28 | 1989-05-01 | Innofinance Altalanos Innovaci | Process for the rapid determination of sperm cell count and/or living sperm count |
EP0358291A2 (en) * | 1988-09-09 | 1990-03-14 | Androscore Corporation | Method of and kit used in testing human males for fertility |
EP0372702A1 (en) * | 1988-10-25 | 1990-06-13 | The Victoria University Of Manchester | Gynaecological procedure |
EP0439893A2 (en) * | 1989-12-26 | 1991-08-07 | United Medical Systems Israel Ltd. | Method and apparatus for measuring the motility of sperm cells |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SE449003B (en) * | 1985-10-18 | 1987-03-30 | Pharmacia Ab | INSTITUTE IN VITRO SEPARATING SPERMIES WITH OWN MUSHLY FORMULATED MEDIUM PENETRATION MEDIUM WHICH DOES NOT INCLUDE HYALURONIC ACID SALT |
-
1992
- 1992-04-02 ES ES9200700A patent/ES2044778B1/en not_active Expired - Fee Related
-
1994
- 1994-06-20 ES ES9401341A patent/ES2094090B1/en not_active Expired - Fee Related
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0302765A1 (en) * | 1987-07-27 | 1989-02-08 | Clonatec | Method for detecting living spermatozoa, and tests therefor |
ES2006560A6 (en) * | 1988-01-28 | 1989-05-01 | Innofinance Altalanos Innovaci | Process for the rapid determination of sperm cell count and/or living sperm count |
EP0358291A2 (en) * | 1988-09-09 | 1990-03-14 | Androscore Corporation | Method of and kit used in testing human males for fertility |
EP0372702A1 (en) * | 1988-10-25 | 1990-06-13 | The Victoria University Of Manchester | Gynaecological procedure |
EP0439893A2 (en) * | 1989-12-26 | 1991-08-07 | United Medical Systems Israel Ltd. | Method and apparatus for measuring the motility of sperm cells |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2721405A1 (en) * | 1994-06-20 | 1995-12-22 | Esteve Carmen Blanco | Testing for sperm fecundity |
ES2387903A1 (en) * | 2010-07-16 | 2012-10-03 | María Albiñana Blanco | Procedure and kit to verify, in a sample of human or animal semen, the existence of fertilizing spermatozoids. (Machine-translation by Google Translate, not legally binding) |
Also Published As
Publication number | Publication date |
---|---|
ES2094090B1 (en) | 1997-09-01 |
ES2044778B1 (en) | 1994-09-01 |
ES2094090A1 (en) | 1997-01-01 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FD1A | Patent lapsed |
Effective date: 20040403 |