ES2044778A1 - Analytical method for determining the fecundating capability of a human semen sample. - Google Patents

Analytical method for determining the fecundating capability of a human semen sample.

Info

Publication number
ES2044778A1
ES2044778A1 ES9200700A ES9200700A ES2044778A1 ES 2044778 A1 ES2044778 A1 ES 2044778A1 ES 9200700 A ES9200700 A ES 9200700A ES 9200700 A ES9200700 A ES 9200700A ES 2044778 A1 ES2044778 A1 ES 2044778A1
Authority
ES
Spain
Prior art keywords
semen sample
fecundating
capability
determining
analytical method
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES9200700A
Other languages
Spanish (es)
Other versions
ES2044778B1 (en
Inventor
Esteve Carmen Blanco
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
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Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to ES9200700A priority Critical patent/ES2044778B1/en
Publication of ES2044778A1 publication Critical patent/ES2044778A1/en
Priority to ES9401341A priority patent/ES2094090B1/en
Application granted granted Critical
Publication of ES2044778B1 publication Critical patent/ES2044778B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Abstract

Analytical method for determining the fecundating capability of a human semen sample. Includes: (a) taking a drop of a solution comprising 0.5%-15% weight hyaluronic acid, 20%-90% weight calcium chloride and 0.1%-5% weight Triton X, spread on a slide and let dry (b) take a previously washed and capacitated semen sample and spread it on the slide previously covered with the solution, cover with a slide cover and leave to incubate for approximately 30 minutes to 48 hours at 25-40 degrees C (c) read the results under a phase contrast microscope, evaluating the halo formed and the size of its diameter. Application in assisted reproduction techniques to forecast the fecundating capability of a human semen sample.
ES9200700A 1992-04-02 1992-04-02 ANALYTICAL METHOD FOR THE DETERMINATION OF THE FECUNDANT CAPACITY OF A SAMPLE OF HUMAN SEMEN. Expired - Fee Related ES2044778B1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
ES9200700A ES2044778B1 (en) 1992-04-02 1992-04-02 ANALYTICAL METHOD FOR THE DETERMINATION OF THE FECUNDANT CAPACITY OF A SAMPLE OF HUMAN SEMEN.
ES9401341A ES2094090B1 (en) 1992-04-02 1994-06-20 ANALYTICAL METHOD FOR THE DETERMINATION OF THE FECUNDANT CAPACITY OF A SAMPLE OF HUMAN SEMEN.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9200700A ES2044778B1 (en) 1992-04-02 1992-04-02 ANALYTICAL METHOD FOR THE DETERMINATION OF THE FECUNDANT CAPACITY OF A SAMPLE OF HUMAN SEMEN.

Publications (2)

Publication Number Publication Date
ES2044778A1 true ES2044778A1 (en) 1994-01-01
ES2044778B1 ES2044778B1 (en) 1994-09-01

Family

ID=8276593

Family Applications (2)

Application Number Title Priority Date Filing Date
ES9200700A Expired - Fee Related ES2044778B1 (en) 1992-04-02 1992-04-02 ANALYTICAL METHOD FOR THE DETERMINATION OF THE FECUNDANT CAPACITY OF A SAMPLE OF HUMAN SEMEN.
ES9401341A Expired - Fee Related ES2094090B1 (en) 1992-04-02 1994-06-20 ANALYTICAL METHOD FOR THE DETERMINATION OF THE FECUNDANT CAPACITY OF A SAMPLE OF HUMAN SEMEN.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES9401341A Expired - Fee Related ES2094090B1 (en) 1992-04-02 1994-06-20 ANALYTICAL METHOD FOR THE DETERMINATION OF THE FECUNDANT CAPACITY OF A SAMPLE OF HUMAN SEMEN.

Country Status (1)

Country Link
ES (2) ES2044778B1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2721405A1 (en) * 1994-06-20 1995-12-22 Esteve Carmen Blanco Testing for sperm fecundity
ES2387903A1 (en) * 2010-07-16 2012-10-03 María Albiñana Blanco Procedure and kit to verify, in a sample of human or animal semen, the existence of fertilizing spermatozoids. (Machine-translation by Google Translate, not legally binding)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0302765A1 (en) * 1987-07-27 1989-02-08 Clonatec Method for detecting living spermatozoa, and tests therefor
ES2006560A6 (en) * 1988-01-28 1989-05-01 Innofinance Altalanos Innovaci Process for the rapid determination of sperm cell count and/or living sperm count
EP0358291A2 (en) * 1988-09-09 1990-03-14 Androscore Corporation Method of and kit used in testing human males for fertility
EP0372702A1 (en) * 1988-10-25 1990-06-13 The Victoria University Of Manchester Gynaecological procedure
EP0439893A2 (en) * 1989-12-26 1991-08-07 United Medical Systems Israel Ltd. Method and apparatus for measuring the motility of sperm cells

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE449003B (en) * 1985-10-18 1987-03-30 Pharmacia Ab INSTITUTE IN VITRO SEPARATING SPERMIES WITH OWN MUSHLY FORMULATED MEDIUM PENETRATION MEDIUM WHICH DOES NOT INCLUDE HYALURONIC ACID SALT

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0302765A1 (en) * 1987-07-27 1989-02-08 Clonatec Method for detecting living spermatozoa, and tests therefor
ES2006560A6 (en) * 1988-01-28 1989-05-01 Innofinance Altalanos Innovaci Process for the rapid determination of sperm cell count and/or living sperm count
EP0358291A2 (en) * 1988-09-09 1990-03-14 Androscore Corporation Method of and kit used in testing human males for fertility
EP0372702A1 (en) * 1988-10-25 1990-06-13 The Victoria University Of Manchester Gynaecological procedure
EP0439893A2 (en) * 1989-12-26 1991-08-07 United Medical Systems Israel Ltd. Method and apparatus for measuring the motility of sperm cells

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2721405A1 (en) * 1994-06-20 1995-12-22 Esteve Carmen Blanco Testing for sperm fecundity
ES2387903A1 (en) * 2010-07-16 2012-10-03 María Albiñana Blanco Procedure and kit to verify, in a sample of human or animal semen, the existence of fertilizing spermatozoids. (Machine-translation by Google Translate, not legally binding)

Also Published As

Publication number Publication date
ES2094090B1 (en) 1997-09-01
ES2044778B1 (en) 1994-09-01
ES2094090A1 (en) 1997-01-01

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Date Code Title Description
FD1A Patent lapsed

Effective date: 20040403