ES2032717A6 - Process for immobilising and/or purifying fusion proteins of bio-technological usefulness in a single step - Google Patents

Process for immobilising and/or purifying fusion proteins of bio-technological usefulness in a single step

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Publication number
ES2032717A6
ES2032717A6 ES9100732A ES9100732A ES2032717A6 ES 2032717 A6 ES2032717 A6 ES 2032717A6 ES 9100732 A ES9100732 A ES 9100732A ES 9100732 A ES9100732 A ES 9100732A ES 2032717 A6 ES2032717 A6 ES 2032717A6
Authority
ES
Spain
Prior art keywords
single step
immobilising
bio
fusion proteins
choline
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES9100732A
Other languages
Spanish (es)
Inventor
Jose Maria Sanchez-Puelles
Morales Jesus Miguel Sanz
Lopez Jose Luis Garcia
Lopez Ernesto Garcia
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
Original Assignee
Consejo Superior de Investigaciones Cientificas CSIC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Superior de Investigaciones Cientificas CSIC filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Priority to ES9100732A priority Critical patent/ES2032717A6/en
Publication of ES2032717A6 publication Critical patent/ES2032717A6/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Process for immobilising and/or purifying fusion proteins of bio-technological usefulness in a single step. The process is based on the use of the property of the terminal carboxyl groups and the pneumococcus LYTA lytic amidase and bean CP-I CPLI muramidase present in Streptococcus pneumoniae to combine specifically with choline or substrates analogous to choline such as diethylaminoethanol (DEAE). A series of plasma vectors have been developed which enable hybrid proteins to be produced through fusion of the DNA fragments of interest and their immobilisation and purification in a single step using affinity chromatography.
ES9100732A 1991-03-22 1991-03-22 Process for immobilising and/or purifying fusion proteins of bio-technological usefulness in a single step Expired - Fee Related ES2032717A6 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9100732A ES2032717A6 (en) 1991-03-22 1991-03-22 Process for immobilising and/or purifying fusion proteins of bio-technological usefulness in a single step

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9100732A ES2032717A6 (en) 1991-03-22 1991-03-22 Process for immobilising and/or purifying fusion proteins of bio-technological usefulness in a single step

Publications (1)

Publication Number Publication Date
ES2032717A6 true ES2032717A6 (en) 1993-02-16

Family

ID=8271747

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9100732A Expired - Fee Related ES2032717A6 (en) 1991-03-22 1991-03-22 Process for immobilising and/or purifying fusion proteins of bio-technological usefulness in a single step

Country Status (1)

Country Link
ES (1) ES2032717A6 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008117259A1 (en) * 2007-01-29 2008-10-02 Universidad Miguel Hernandez Protein domains with choline affinity for improving expression, immobilization and purification of polypeptides

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008117259A1 (en) * 2007-01-29 2008-10-02 Universidad Miguel Hernandez Protein domains with choline affinity for improving expression, immobilization and purification of polypeptides
ES2321788A1 (en) * 2007-01-29 2009-06-10 Universidad Miguel Hernandez Protein domains with choline affinity for improving expression, immobilization and purification of polypeptides

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Effective date: 20110610