ES2029785T3 - Procedimiento para producir factor de crecimiento tipo insulina i y plasmido para su produccion. - Google Patents

Procedimiento para producir factor de crecimiento tipo insulina i y plasmido para su produccion.

Info

Publication number
ES2029785T3
ES2029785T3 ES198686114321T ES86114321T ES2029785T3 ES 2029785 T3 ES2029785 T3 ES 2029785T3 ES 198686114321 T ES198686114321 T ES 198686114321T ES 86114321 T ES86114321 T ES 86114321T ES 2029785 T3 ES2029785 T3 ES 2029785T3
Authority
ES
Spain
Prior art keywords
plasmide
procedure
production
growth factor
fused
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES198686114321T
Other languages
English (en)
Inventor
Ikuo Ueda
Mineo Niwa
Susumu Satoh
Yoshimasa Saitoh
Chihiro Kusunoki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujisawa Pharmaceutical Co Ltd
Original Assignee
Fujisawa Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB858525929A external-priority patent/GB8525929D0/en
Priority claimed from GB868617073A external-priority patent/GB8617073D0/en
Application filed by Fujisawa Pharmaceutical Co Ltd filed Critical Fujisawa Pharmaceutical Co Ltd
Application granted granted Critical
Publication of ES2029785T3 publication Critical patent/ES2029785T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g.hCG [human chorionic gonadotropin]; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Endocrinology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Microbiology (AREA)
  • Reproductive Health (AREA)
  • Diabetes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PROCEDIMIENTO PARA PREPARAR FACTOR I DEL CRECIMIENTO DE TIPO INSULINICO (IGF- I) UNIDO A UN PEPTIDO PROTECTOR POR (1) CULTIVO DE UN MICROORGANISMO TRANSFORMADO CON UN PLASMIDO DE EXPRESION QUE TIENE UN IGF-1 DE CODIFICACION GENETICA UNIDO CON UN PEPTIDO PROTECTOR Y DEL CUAL SE SEPARA UN GEN EN CUERDA, Y (2) RECUPERACION DEL IGF-1 UNIDO CON UN PEPTIDO PROTECTOR DEL CALDO DE CULTIVO, UN PLASMIDO DE EXPRESION QUE TIENE UN IGF-1 DE CODIFICADOR DE GENES UNIDO CON UN PEPTIDO PROTECTOR Y DEL CUAL SE SEPARA UN GEN EN CUERDA. SE DESCRIBEN TAMBIEN PLASMIDOS UTILES PARA DICHO PROCESO.
ES198686114321T 1985-10-21 1986-10-16 Procedimiento para producir factor de crecimiento tipo insulina i y plasmido para su produccion. Expired - Lifetime ES2029785T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB858525929A GB8525929D0 (en) 1985-10-21 1985-10-21 Production of insulin-like growth factor
GB868617073A GB8617073D0 (en) 1986-07-14 1986-07-14 Insulin-like growth factor i

Publications (1)

Publication Number Publication Date
ES2029785T3 true ES2029785T3 (es) 1992-10-01

Family

ID=26289914

Family Applications (1)

Application Number Title Priority Date Filing Date
ES198686114321T Expired - Lifetime ES2029785T3 (es) 1985-10-21 1986-10-16 Procedimiento para producir factor de crecimiento tipo insulina i y plasmido para su produccion.

Country Status (10)

Country Link
EP (1) EP0219814B1 (es)
JP (1) JPH07114702B2 (es)
KR (1) KR880001813A (es)
AT (1) ATE67243T1 (es)
CA (1) CA1297814C (es)
DE (1) DE3681398D1 (es)
DK (1) DK502786A (es)
ES (1) ES2029785T3 (es)
GR (1) GR3002696T3 (es)
IL (1) IL80376A0 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988005078A1 (en) * 1986-12-31 1988-07-14 Lucky, Ltd. Method for the production of bovine growth hormone using a synthetic gene
US7049102B1 (en) 1989-09-22 2006-05-23 Board Of Trustees Of Leland Stanford University Multi-gene expression profile
KR920003787B1 (ko) * 1990-03-05 1992-05-14 한국과학기술 연구원 신규 플라스미드 제조 및 그를 함유한 균주를 배양하여 아이 지 에프-1(igf-1)을 생산하는 방법
JP2574549B2 (ja) * 1991-03-08 1997-01-22 萩原 義秀 新規プラスミド
US5288931A (en) * 1991-12-06 1994-02-22 Genentech, Inc. Method for refolding insoluble, misfolded insulin-like growth factor-I into an active conformation
US5663304A (en) * 1993-08-20 1997-09-02 Genentech, Inc. Refolding of misfolded insulin-like growth factor-I
EP0779927A1 (en) * 1994-09-08 1997-06-25 Chiron Corporation A method of improved production of insulin-like growth factor

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0561137B1 (en) * 1983-04-25 2002-07-03 Chiron Corporation Hybrid DNA Synthesis of Mature Insulin-like Growth Factors
IL71991A (en) * 1983-06-06 1994-05-30 Genentech Inc Preparation of human FGI and FGE in their processed form through recombinant AND tranology in prokaryotes
SE8303626D0 (sv) * 1983-06-23 1983-06-23 Kabigen Ab A recombinant plasmid a transformant microorganism, a polydoxyrebonucleotide segment, a process for producing a biologically active protein, and the protein thus produced
WO1985000831A1 (en) * 1983-08-10 1985-02-28 Amgen Microbial expression of insulin-like growth factor
HU194307B (en) * 1983-09-16 1988-01-28 Richter Gedeon Vegyeszet Process for producing plasmidvectors of high copy number
DK108685A (da) * 1984-03-19 1985-09-20 Fujisawa Pharmaceutical Co Vaekstfaktor i

Also Published As

Publication number Publication date
DK502786D0 (da) 1986-10-20
DE3681398D1 (de) 1991-10-17
CA1297814C (en) 1992-03-24
DK502786A (da) 1987-04-22
EP0219814A1 (en) 1987-04-29
EP0219814B1 (en) 1991-09-11
ATE67243T1 (de) 1991-09-15
KR880001813A (ko) 1988-04-27
JPH07114702B2 (ja) 1995-12-13
JPS62175192A (ja) 1987-07-31
GR3002696T3 (en) 1993-01-25
IL80376A0 (en) 1987-01-30

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