ES2020021A6 - Un procedimiento para la preparacion de un dna o un fragmento de restriccion derivado del mismo que contiene un gen codificante de resistencia a doxorrubicina - Google Patents

Un procedimiento para la preparacion de un dna o un fragmento de restriccion derivado del mismo que contiene un gen codificante de resistencia a doxorrubicina

Info

Publication number
ES2020021A6
ES2020021A6 ES8901800A ES8901800A ES2020021A6 ES 2020021 A6 ES2020021 A6 ES 2020021A6 ES 8901800 A ES8901800 A ES 8901800A ES 8901800 A ES8901800 A ES 8901800A ES 2020021 A6 ES2020021 A6 ES 2020021A6
Authority
ES
Spain
Prior art keywords
doxorubicin
resistance
dna
derived therefrom
gene coding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES8901800A
Other languages
English (en)
Inventor
Marinella Caruso
Anna Luisa Colombo
Luisa Garofano
Francesca Torti
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pfizer Italia SRL
Original Assignee
Farmitalia Carlo Erba SRL
Carlo Erba SpA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Farmitalia Carlo Erba SRL, Carlo Erba SpA filed Critical Farmitalia Carlo Erba SRL
Publication of ES2020021A6 publication Critical patent/ES2020021A6/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

UN PROCEDIMIENTO PARA LA PREPARACION DE UN DNA O UN FRAGMENTO DE RESTRICCION DERIVADO DEL MISMO QUE CONTIENE UN GEN CODIFICANTE DE RESISTENCIA O DOXORRUBICINA. COMPRENDE: A) PREPARAR UN BANCO DE DNA GENOMICO DE S. PEUCETIUS M76 (D.S.M. 4592) O DE UNA CEPA DERIVADA DE ELLA; B) SELECCIONAR DICHO BANCO PARA RESISTENCIA A DOXORRUBICINA; C) OBTENER UN DNA INSERTO DE UN VECTOR RECOMBINANTE QUE FORMA PARTE DEL BANCO Y QUE HA RESULTADO POSITIVO PARA RESISTENCIA A DOXORRUBICINA; D) OPCIONALMENTE, OBTENER DEL DNA INSERTO UN FRAGMENTO DE RETRICCION QUE CONTIENE EL GEN CODIFICANTE DE RESISTENCIA O DOXORRUBICINA. TIENE UTILIDAD PORQUE PERMITE CONFERIOR RESISTENCIA A DOXORRUBICINA EN UN MICROORGANISMO PRODUCTOR DE LA MISMA, LO QUE AUMENTA LA PRODUCTIVIDAD DE LA CEPA.
ES8901800A 1988-05-27 1989-05-26 Un procedimiento para la preparacion de un dna o un fragmento de restriccion derivado del mismo que contiene un gen codificante de resistencia a doxorrubicina Expired - Fee Related ES2020021A6 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT1269788 1988-05-27

Publications (1)

Publication Number Publication Date
ES2020021A6 true ES2020021A6 (es) 1991-07-16

Family

ID=11142787

Family Applications (1)

Application Number Title Priority Date Filing Date
ES8901800A Expired - Fee Related ES2020021A6 (es) 1988-05-27 1989-05-26 Un procedimiento para la preparacion de un dna o un fragmento de restriccion derivado del mismo que contiene un gen codificante de resistencia a doxorrubicina

Country Status (1)

Country Link
ES (1) ES2020021A6 (es)

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Legal Events

Date Code Title Description
PC1A Transfer granted
FD1A Patent lapsed

Effective date: 20070528