ES2017024A6 - Method for transforming plants via the shoot apex. - Google Patents

Method for transforming plants via the shoot apex.

Info

Publication number
ES2017024A6
ES2017024A6 ES898901864A ES8901864A ES2017024A6 ES 2017024 A6 ES2017024 A6 ES 2017024A6 ES 898901864 A ES898901864 A ES 898901864A ES 8901864 A ES8901864 A ES 8901864A ES 2017024 A6 ES2017024 A6 ES 2017024A6
Authority
ES
Spain
Prior art keywords
transforming
tissue
transforming plants
cultivating
shoot apex
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES898901864A
Other languages
Spanish (es)
Inventor
Roberta H Smith
Jean H Gould
Eugenio C Ulian
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Texas A&M University System
Original Assignee
Texas A&M University System
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Texas A&M University System filed Critical Texas A&M University System
Publication of ES2017024A6 publication Critical patent/ES2017024A6/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

It consists in cultivating a target tissue of meristem from extirpated buds in a suitable growth medium, inoculating said tissue with a suitable vector capable of transforming it, said vector being an Agrobacterium tumefaciens which contains a hyperverulent binary strain, and cultivating the transformed plant tissue to induce the growth of roots. The invention is useful in reducing the risk of somaclonal variation when transforming target plants selected among petunias, soybeans, alfalfa, sunflower, cotton, wheat, corn and others.
ES898901864A 1988-06-01 1989-05-31 Method for transforming plants via the shoot apex. Expired - Fee Related ES2017024A6 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US20156888A 1988-06-01 1988-06-01
US32266089A 1989-03-13 1989-03-13

Publications (1)

Publication Number Publication Date
ES2017024A6 true ES2017024A6 (en) 1990-12-16

Family

ID=26896880

Family Applications (1)

Application Number Title Priority Date Filing Date
ES898901864A Expired - Fee Related ES2017024A6 (en) 1988-06-01 1989-05-31 Method for transforming plants via the shoot apex.

Country Status (12)

Country Link
US (1) US5164310A (en)
EP (1) EP0419533A1 (en)
JP (1) JP2996995B2 (en)
CN (1) CN1042638A (en)
AU (1) AU3756889A (en)
DK (1) DK176062B1 (en)
ES (1) ES2017024A6 (en)
IE (1) IE65516B1 (en)
IL (1) IL90440A (en)
NZ (1) NZ229340A (en)
PT (1) PT90709B (en)
WO (1) WO1989012102A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2160019A1 (en) * 1998-09-11 2001-10-16 Consejo Superior Investigacion Propagation of e.g. tomato with genetic modification comprises of meristem removal and feed of nutrients and growth regulators via the cotyledon and root

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