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Laboratorios Hubber SA
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Laboratorios Hubber SA
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Application filed by Laboratorios Hubber SAfiledCriticalLaboratorios Hubber SA
Priority to ES8601429ApriorityCriticalpatent/ES2002118A6/en
Publication of ES2002118A6publicationCriticalpatent/ES2002118A6/en
The factor IX suitable for prolonged heat treatment to inactivate contaminating viruses, is made from fresh frozen plasma, is (a) subjected to a thawing process to obtain a cryo-ppte (b) the ppte. is sepd. by centrifuging (c) the supernatant liq. is treated with an anionic exchange agent to absorb the coagulation factors (II), (VII), (IX) and (X) and sepd. from the liq. (d) the factors are eluted with a buffer which removes a min. amt. of other proteins (e) the elute is diluted with dist. water and PEG added to ppte. part of the protein impurities and activated coagulation factors which may have been formed (f) the Factor IX is purified and conc. by a further PEG addn. and (g) it is washed with a suitable buffer soln.
ES8601429A1986-08-281986-08-28High-purity blood factor IX prodn.
ExpiredES2002118A6
(en)