EP4784792A1 - Méthodes et compositions pour produire des banques de séquençage - Google Patents

Méthodes et compositions pour produire des banques de séquençage

Info

Publication number
EP4784792A1
EP4784792A1 EP24873686.0A EP24873686A EP4784792A1 EP 4784792 A1 EP4784792 A1 EP 4784792A1 EP 24873686 A EP24873686 A EP 24873686A EP 4784792 A1 EP4784792 A1 EP 4784792A1
Authority
EP
European Patent Office
Prior art keywords
sequencing
nucleic acids
sequencing adapter
fragment
adapter
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP24873686.0A
Other languages
German (de)
English (en)
Inventor
Joshua KAPP
Richard E. Green
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California
University of California Berkeley
University of California, San Diego
Original Assignee
University of California
University of California Berkeley
University of California, San Diego
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of California, University of California Berkeley, University of California, San Diego filed Critical University of California
Publication of EP4784792A1 publication Critical patent/EP4784792A1/fr
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des procédés de production d'une banque de séquençage à partir d'un échantillon comprenant des acides nucléiques. Dans certains modes de réalisation, les procédés comprennent l'amplification d'acides nucléiques de l'échantillon à l'aide d'une amorce sens comprenant un domaine 3' complémentaire à des acides nucléiques dans l'échantillon et un domaine 5' comprenant un fragment d'un premier adaptateur de séquençage, ainsi qu'une amorce antisens comprenant un domaine 3' complémentaire des acides nucléiques dans l'échantillon et un domaine 5' comprenant un fragment d'un second adaptateur de séquençage. L'amplification produit des amplicons comprenant le fragment du premier adaptateur de séquençage au niveau d'une première extrémité et le fragment du second adaptateur de séquençage au niveau de la seconde extrémité. Les procédés comprennent en outre la liaison covalente du reste du second adaptateur de séquençage au fragment du second adaptateur de séquençage, de telle sorte qu'une bibliothèque de séquençage est produite dans laquelle des modèles séquencés sont uniquement ceux dérivés des amplicons.
EP24873686.0A 2023-09-28 2024-09-27 Méthodes et compositions pour produire des banques de séquençage Pending EP4784792A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363541181P 2023-09-28 2023-09-28
PCT/US2024/048882 WO2025072689A1 (fr) 2023-09-28 2024-09-27 Méthodes et compositions pour produire des banques de séquençage

Publications (1)

Publication Number Publication Date
EP4784792A1 true EP4784792A1 (fr) 2026-08-05

Family

ID=95202147

Family Applications (1)

Application Number Title Priority Date Filing Date
EP24873686.0A Pending EP4784792A1 (fr) 2023-09-28 2024-09-27 Méthodes et compositions pour produire des banques de séquençage

Country Status (2)

Country Link
EP (1) EP4784792A1 (fr)
WO (1) WO2025072689A1 (fr)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019191900A1 (fr) * 2018-04-03 2019-10-10 Burning Rock Biotech Compositions et procédés de préparation de bibliothèques d'acides nucléiques
EP3827011B1 (fr) * 2018-07-24 2024-06-19 Salish Bioscience Inc. Procédés et composition pour analyse génomique ciblée
WO2020206143A1 (fr) * 2019-04-05 2020-10-08 Claret Bioscience, Llc Procédés et compositions d'analyse d'acide nucléique

Also Published As

Publication number Publication date
WO2025072689A1 (fr) 2025-04-03

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