EP4775665A2 - Genetisch veränderte clostridium-strains und verwendungen davon - Google Patents

Genetisch veränderte clostridium-strains und verwendungen davon

Info

Publication number
EP4775665A2
EP4775665A2 EP26165288.7A EP26165288A EP4775665A2 EP 4775665 A2 EP4775665 A2 EP 4775665A2 EP 26165288 A EP26165288 A EP 26165288A EP 4775665 A2 EP4775665 A2 EP 4775665A2
Authority
EP
European Patent Office
Prior art keywords
clostra
clostridium
strain
gene
strains
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP26165288.7A
Other languages
English (en)
French (fr)
Inventor
Aleksandra Maria KUBIAK
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Livingmed Biotech Srl
Original Assignee
Livingmed Biotech Srl
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Livingmed Biotech Srl filed Critical Livingmed Biotech Srl
Publication of EP4775665A2 publication Critical patent/EP4775665A2/de
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/36—Adaptation or attenuation of cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/742—Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N3/00—Spore forming or isolating processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/145—Clostridium

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Mycology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP26165288.7A 2019-12-18 2020-12-18 Genetisch veränderte clostridium-strains und verwendungen davon Pending EP4775665A2 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201962949480P 2019-12-18 2019-12-18
EP19217672 2019-12-18
PCT/EP2020/087338 WO2021123391A1 (en) 2019-12-18 2020-12-18 Genetically modified clostridium strains and uses thereof
EP20848793.4A EP4077359B1 (de) 2019-12-18 2020-12-18 Genetisch veränderte clostridium-strains und verwendungen davon

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
EP20848793.4A Division EP4077359B1 (de) 2019-12-18 2020-12-18 Genetisch veränderte clostridium-strains und verwendungen davon

Publications (1)

Publication Number Publication Date
EP4775665A2 true EP4775665A2 (de) 2026-07-15

Family

ID=76477127

Family Applications (2)

Application Number Title Priority Date Filing Date
EP26165288.7A Pending EP4775665A2 (de) 2019-12-18 2020-12-18 Genetisch veränderte clostridium-strains und verwendungen davon
EP20848793.4A Active EP4077359B1 (de) 2019-12-18 2020-12-18 Genetisch veränderte clostridium-strains und verwendungen davon

Family Applications After (1)

Application Number Title Priority Date Filing Date
EP20848793.4A Active EP4077359B1 (de) 2019-12-18 2020-12-18 Genetisch veränderte clostridium-strains und verwendungen davon

Country Status (4)

Country Link
US (1) US20240254434A1 (de)
EP (2) EP4775665A2 (de)
CN (2) CN115244069A (de)
WO (1) WO2021123391A1 (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022171904A1 (en) * 2021-02-15 2022-08-18 Livingmed Biotech S.R.L. Genetically modified clostridium strains expressing recombinant antigens and uses thereof

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003045153A1 (en) 2001-11-21 2003-06-05 The Johns Hopkins University Combination bacteriolytic therapy for the treatment of tumors
WO2003074681A1 (en) 2002-03-07 2003-09-12 Royal Holloway & Bedford New College University Of London Recombinant spores
WO2007149433A2 (en) 2006-06-19 2007-12-27 The Johns Hopkins University Tumor-specific delivery of therapeutic agents via liposomase
WO2009111177A2 (en) 2008-03-05 2009-09-11 Mount Sinai School Of Medicine Of New York University Compositions and methods comprising genetically enhanced obligate and facultative anaerobic bacteria for oncopathic cancer therapy
WO2010005722A2 (en) 2008-06-16 2010-01-14 Northwestern University Methods and compositions for generating sporulation deficient bacteria
WO2013159155A1 (en) 2012-04-27 2013-10-31 Griffith University Oncolytic clostridium ghonii strains, and methods of production and use
WO2015075475A1 (en) 2013-11-22 2015-05-28 The University Of Nottingham Treatment for cancer
WO2016095503A1 (zh) 2014-12-19 2016-06-23 山东创新药物研发有限公司 一株戈氏梭菌驯化株的应用
WO2017064439A1 (fr) 2015-10-16 2017-04-20 IFP Energies Nouvelles Outil genetique de transformation de bacteries clostridium
WO2017190257A1 (en) 2016-05-01 2017-11-09 Neemo Inc Harnessing heterologous and endogenous crispr-cas machineries for efficient markerless genome editing in clostridium

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002000232A2 (en) * 2000-06-26 2002-01-03 Maxygen, Inc. Methods and compositions for developing spore display systems for medicinal and industrial applications
US8512982B2 (en) * 2007-12-21 2013-08-20 Danisco Us Inc. Enhanced protein production in Bacillus
EP2588614A1 (de) * 2010-06-30 2013-05-08 DSM IP Assets B.V. Sporenoberflächendisplay bioaktiver moleküle
US20130224757A1 (en) * 2010-08-19 2013-08-29 Novozymes A/S Induced sporulation screening method
US20140274800A1 (en) * 2013-03-13 2014-09-18 Procarta Biosystems Ltd. Transcription factor decoys for the treatment and prevention of infections caused by bacteria including clostridium difficile
WO2017074257A1 (en) * 2015-10-26 2017-05-04 Nanyang Technological University Non-viable derivatives of clostridium sporogenes as anti-cancer therapeutic agents
EP3345918A1 (de) * 2017-01-05 2018-07-11 Institut National De La Recherche Agronomique Verwendung des cpcr-regulatorgens für die erhöhung von neuen rekombinanten stämmen von bacillus-thuringiensis mit reduzierter sporulierungskapazität
US20230193241A1 (en) * 2017-06-25 2023-06-22 Snipr Technologies Limited Altering microbial populations & modifying microbiota
FR3081881B1 (fr) * 2018-06-04 2024-05-24 Ifp Energies Now Outil genetique optimise pour modifier les bacteries du genre clostridium
GB201901166D0 (en) * 2019-01-28 2019-03-20 Univ Nottingham Genetic construct and uses thereof
GB202019767D0 (en) * 2020-12-15 2021-01-27 Chain Biotechnology Ltd Compostitions and methods

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003045153A1 (en) 2001-11-21 2003-06-05 The Johns Hopkins University Combination bacteriolytic therapy for the treatment of tumors
WO2003074681A1 (en) 2002-03-07 2003-09-12 Royal Holloway & Bedford New College University Of London Recombinant spores
WO2007149433A2 (en) 2006-06-19 2007-12-27 The Johns Hopkins University Tumor-specific delivery of therapeutic agents via liposomase
WO2009111177A2 (en) 2008-03-05 2009-09-11 Mount Sinai School Of Medicine Of New York University Compositions and methods comprising genetically enhanced obligate and facultative anaerobic bacteria for oncopathic cancer therapy
WO2010005722A2 (en) 2008-06-16 2010-01-14 Northwestern University Methods and compositions for generating sporulation deficient bacteria
WO2013159155A1 (en) 2012-04-27 2013-10-31 Griffith University Oncolytic clostridium ghonii strains, and methods of production and use
WO2015075475A1 (en) 2013-11-22 2015-05-28 The University Of Nottingham Treatment for cancer
WO2016095503A1 (zh) 2014-12-19 2016-06-23 山东创新药物研发有限公司 一株戈氏梭菌驯化株的应用
WO2017064439A1 (fr) 2015-10-16 2017-04-20 IFP Energies Nouvelles Outil genetique de transformation de bacteries clostridium
WO2017190257A1 (en) 2016-05-01 2017-11-09 Neemo Inc Harnessing heterologous and endogenous crispr-cas machineries for efficient markerless genome editing in clostridium

Non-Patent Citations (63)

* Cited by examiner, † Cited by third party
Title
"UniProt", Database accession no. P40933
AGRAWAL SKANDIMALLA E, IMMUNO-ONCOL TECH., vol. 3, 2019, pages 15 - 23
ALEXAKI A. ET AL., J MOL BIOL, vol. 431, 2019, pages 2434 - 2441
AL-HINAI M ET AL., MICROBIOL MOL BIOL REV., vol. 79, 2015, pages 19 - 37
ATMADJAJA A ET AL., FEMS MICROBIOL LETT., vol. 366, 2019, pages 059
BANJEREE A ET AL., APPL ENVIRON MICROBIOL., vol. 80, 2014, pages 2410 - 2416
BANNAM TL ET AL., MOL MICROBIOL., vol. 16, 1995, pages 535 - 51
BRUDER M ET AL., APPL ENVIRON MICROBIOL., vol. 82, 2016, pages 6109 - 6119
CANADAS I ET AL., ACS SYNTH BIOL., vol. 8, 2019, pages 1379 - 1390
COLLINS ME ET AL., J GEN MICROBIOL., vol. 131, 1985, pages 2097 - 105
COOKSLEY CM ET AL., APPL ENVIRON MICROBIOL., vol. 76, 2010, pages 7881 - 60
CORRALIZA-GORJÓN I ET AL., FRONT IMMUNOL., vol. 8, 2017, pages 1804
CRUZ-MORALES P ET AL., GENOME BIOL. EVOL., vol. 11, no. 7, 2019, pages 2035
CUI W ET AL., MICROB CELL FACT., vol. 15, 2016, pages 199
DEMBEK M ET AL., FRONT MICROBIOL., vol. 8, 2017, pages 1793
DIALLO M ET AL., METHODS, 2019, pages 1046 - 2023
DONG H ET AL., METAB ENGIN., vol. 14, 2012, pages 59 - 67
EHSAAN M ET AL., BIOTECHNOL BIOFUEL., vol. 9, 2016, pages 4
ELENEEN YCOLEN RR, ADV EXP MED BIOL., vol. 995, 2017, pages 141 - 153
EMPTAGE CD ET AL., BIOCHEM PHARMACOL., vol. 77, no. 1, 2009, pages 21 - 9
FOULQUIER C ET AL., BIOTECHNOL BIOFUEL., vol. 12, 2019, pages 31
GAO W ET AL., BIOTECHNOL PROG., vol. 20, 2004, pages 443 - 8
GROOT AJ ET AL., BIOCHEM BIOPHYS RES COMMUN., vol. 364, 2007, pages 985 - 9
GROTE A ET AL., NUCLEIC ACIDS RES., vol. 33, 2005, pages 526 - 31
HAMADA Y, BIOORG MED CHEM LETT., vol. 27, 2017, pages 1627 - 1632
HARTMAN AH ET AL., APPL ENVIRON MICROBIOL., vol. 77, 2011, pages 8439 - 41
HEAP J ET AL., J MICROBIOL METHODS, vol. 78, 2009, pages 79 - 85
HEAP J ET AL., ONCOTARGET, vol. 5, 2014, pages 1761 - 9
HUANG H ET AL., ACS SYNTH. BIOL., vol. 12, 2016, pages 1355 - 1361
INGLE P ET AL., SCI REP., vol. 9, 2019, pages 8123
JINEK M ET AL., SCIENCE, vol. 337, 2012, pages 816 - 21
JOSEPH R ET AL., FRONT. MICROBIOL., vol. 9, 2018, pages 154
KNOX R, BIOCHEM PHARMACOL., vol. 37, 1988, pages 4671 - 7
KUBIAK AM ET AL., GENOME ANNOUNC., vol. 3, 2015, pages e00942 - 15
KUBIAK AMMINTON NP, RES MICROBIOL., vol. 66, 2015, pages 244 - 54
KWON SW ET AL., FRONT BIOENG BIOTECHNOL., vol. 8, 2020, pages 282
LOVITT RW ET AL., J APPL BACTERIOL., vol. 62, 1987, pages 81 - 92
MCALLISTER KNSORG JA, J BACTERIOL., vol. 201, 2019, pages 00219 - 19
MINTON N ET AL., ANAEROBE, vol. 41, 2016, pages 104 - 112
MINTON NMORRIS J, J GEN MICROBIOL., vol. 127, 1981, pages 325 - 331
MOWDAY A ET AL., CANCERS, vol. 8, 2016, pages 63
NI G ET AL., BIOMED RES INT., 2019, pages 1395138
NORA L ET AL., BIOTECHNOL ADV., vol. 3, 2019, pages 107433
PURDY D ET AL., MOL MICROBIOL., vol. 46, 2002, pages 439 - 52
PYNE M ET AL., SCI REP., vol. 6, 2016, pages 25666
RAUTIO J ET AL., NAT REV DRUG DISCOV., vol. 17, 2018, pages 559 - 587
ROSSI S ET AL., EUR J NUCL MED MOL IMAGING., vol. 44, 2017, pages 2310 - 2325
SCHWARZ K ET AL., J MICROBIOL METHODS., vol. 68, 2007, pages 396 - 402
SHEN S ET AL., MICROBIOL SPECTR., vol. 7, 2019
SUBBIAH V ET AL., DIAGNOSTICS, vol. 7, 2017, pages E10
TANNER R ET AL., CURRENT MICROBIOL., vol. 5, 1981, pages 35 - 38
THEYS J ET AL., BRITISH JOURNAL OF CANCER, vol. 95, 2006, pages 1212 - 1219
TORRES W ET AL., J CANCER METASTASIS TREAT, vol. 4, 2018, pages 4
TOTTEN PA ET AL., INFECT IMMUN., vol. 63, 1995, pages 4409 - 16
WANG S ET AL., CLIN MICROBIOL INFEC., vol. 24, 2018, pages 1095 - 1099
WASELS F ET AL., J MICROBIOL METHODS, vol. 140, 2017, pages 5 - 11
WEN Z ET AL., METAB ENG., vol. 39, 2017, pages 38 - 48
WILLIAMS EM ET AL., BIOCHEM J., vol. 471, 2015, pages 131 - 53
XIN Y ET AL., NAT REV DRUG DISCOV., vol. 18, 2019, pages 899 - 90
YANG WW ET AL., J APPL MICROBIOL., vol. 106, 2009, pages 27 - 33
ZHANG J ET AL., BIOTECHNOL BIOFUELS, vol. 8, 2015, pages 36
ZHANG YL ET AL., LETT APPL MICROBIOL., vol. 59, 2014, pages 580 - 6
ZHONG S ET AL., TRANSL ONCOL., vol. 13, 2019, pages 57 - 69

Also Published As

Publication number Publication date
CN121006308A (zh) 2025-11-25
EP4077359B1 (de) 2026-03-18
EP4077359A1 (de) 2022-10-26
WO2021123391A1 (en) 2021-06-24
CN115244069A (zh) 2022-10-25
EP4077359C0 (de) 2026-03-18
US20240254434A1 (en) 2024-08-01

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION HAS BEEN PUBLISHED

AC Divisional application: reference to earlier application

Ref document number: 4077359

Country of ref document: EP

Kind code of ref document: P

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

PUAL Search report despatched

Free format text: ORIGINAL CODE: 0009013