EP4754288A2 - Mutante dna-polymerasen und verfahren zur verwendung - Google Patents

Mutante dna-polymerasen und verfahren zur verwendung

Info

Publication number
EP4754288A2
EP4754288A2 EP24762069.3A EP24762069A EP4754288A2 EP 4754288 A2 EP4754288 A2 EP 4754288A2 EP 24762069 A EP24762069 A EP 24762069A EP 4754288 A2 EP4754288 A2 EP 4754288A2
Authority
EP
European Patent Office
Prior art keywords
dna polymerase
dna
nucleic acid
polymerase
polymerases
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP24762069.3A
Other languages
English (en)
French (fr)
Inventor
Achim Karger
Remigijus Skirgaila
Dominyka ERLECKIEN
Gintar TAULAVI I T
Karolina MUKAUSKAIT
Anushka JIRAPURE
Ramandeep Kaur
Jolanta VITKUT
Anadelia VALLADARES POPOCA
Linlin Huang
Carole Bornarth
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Publication of EP4754288A2 publication Critical patent/EP4754288A2/de
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP24762069.3A 2023-07-28 2024-07-29 Mutante dna-polymerasen und verfahren zur verwendung Pending EP4754288A2 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US202363516396P 2023-07-28 2023-07-28
US202363516412P 2023-07-28 2023-07-28
US202363516435P 2023-07-28 2023-07-28
PCT/US2024/039979 WO2025029707A2 (en) 2023-07-28 2024-07-29 Mutant dna polymerases and methods of use

Publications (1)

Publication Number Publication Date
EP4754288A2 true EP4754288A2 (de) 2026-06-10

Family

ID=92543342

Family Applications (1)

Application Number Title Priority Date Filing Date
EP24762069.3A Pending EP4754288A2 (de) 2023-07-28 2024-07-29 Mutante dna-polymerasen und verfahren zur verwendung

Country Status (3)

Country Link
EP (1) EP4754288A2 (de)
CN (1) CN122095103A (de)
WO (1) WO2025029707A2 (de)

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4554101A (en) 1981-01-09 1985-11-19 New York Blood Center, Inc. Identification and preparation of epitopes on antigens and allergens on the basis of hydrophilicity
US5079352A (en) 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
AU2003269991A1 (en) * 2003-08-20 2005-03-10 Applera Corporation Polymerase compositions
US20060223067A1 (en) * 2005-03-31 2006-10-05 Paolo Vatta Mutant DNA polymerases and methods of use
WO2020037295A1 (en) * 2018-08-17 2020-02-20 The Regents Of The University Of California Enhanced speed polymerases for sanger sequencing
CN113597468B (zh) * 2019-03-13 2024-09-13 武汉爱博泰克生物科技有限公司 用于快速扩增的突变型Taq聚合酶

Also Published As

Publication number Publication date
WO2025029707A2 (en) 2025-02-06
CN122095103A (zh) 2026-05-26
WO2025029707A3 (en) 2025-04-24

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