EP4643984A1 - Laboratory shaker and method for treating the interior of the laboratory shaker - Google Patents

Abstract

The invention relates to a laboratory shaker (1) for shaking sample vessels (130) mounted on a platform within a chamber (2) with bottom openings (35) for the passage of coupling rods which couple the platform to a drive, wherein the bottom openings are sealed by a flat sealing element (50), thereby maximizing the available usable volume in the interior of the chamber.

Description

The invention relates to laboratory shakers for shaking laboratory samples stored in sample containers, in particular microorganisms in suspension, preferably mammalian cells in suspension.

Temperature-controlled laboratory shakers are used in biological, medical, and pharmaceutical laboratories for cultivating cells, bacteria, yeast, and other organisms in suspension. They are essential, for example, for the production of recombinant DNA, protein expression, or the screening of cultures. Since a laboratory shaker is primarily a shared piece of equipment that runs continuously at high speeds and under variable loads, it must be durable and reliable.

The key parameters relevant to a user of a laboratory shaker, especially an incubation shaker, are a specific target temperature in the sample storage chamber, a specific speed, and a corresponding load-bearing capacity of the shaker platform, as well as the ability to control the CO2 concentration and humidity of the incubation atmosphere. Most applications require the use of different vessel sizes and types: from plates for initial screening to conical vessels for precultures and large flasks for plasmid production or protein expression. The constant demand for higher product yields has led to the invention of new flask types that offer better aeration than standard shake flasks. This allows the typical filling volume to be increased by up to 40%, resulting in a higher weight on the platform. Higher rotational speeds above 250 rpm are common for applications with, for example, BE coli, to achieve a significant increase in cell density. When shaking sample plates for screening, it is important to achieve complete mixing and prevent cell sedimentation. The requirements for laboratory shakers are therefore extensive, and in addition to durability, they include sufficient versatility to handle all types of... The system must handle platform configurations, loads, and high speeds. Durability and robustness are required for reliable operation over many years.

An orbital shaker is a laboratory shaker in which a platform is moved in an elliptical or circular path, with the movement controlled by an eccentric. Generally, beakers, flasks, and other vessels are attached to the top of the platform, causing the liquid inside to swirl around the inner walls of the vessel to increase mixing and improve the interaction or exchange between the liquid and the local gaseous environment.

Orbital shakers are also specifically laboratory shakers that move a platform such that all points on the platform move integrally in a common, planar, orbital path, as a superposition of two translations, with the amplitude defined by an eccentric. Motion in a common, planar, orbital path means, in particular, that all points on the orbital shaker's platform move in an elliptical or circular path, the path lying in a plane. A "superposition of two translations" refers specifically to the fact that the shaker's platform motion can be considered a combination of two linear motions in different directions. This superposition results in the elliptical or circular path. An eccentric is a device used to convert a translational motion into a rotational motion. In this case, the eccentric defines the amplitude of the motion, i.e., the maximum displacement of the platform. In mechanics, an eccentric is a control disc that is mounted on a shaft and whose center point is located outside the axis of the shaft.

Such laboratory shakers have a chamber for holding the laboratory samples to be tempered; this chamber is usually located inside a housing. Access to the chamber, through which the user places and removes the samples inside the housing, particularly within the chamber, is generally via a housing opening that can be closed by means of a housing door. In further Some models of these chambers also include a gas supply. These types of devices allow the cultivation of cells in a _CO₂ atmosphere. They are called incubation shakers. A well-known laboratory shaker is the ^Innova® S44i, available from Eppendorf SE, Hamburg, Germany.

In a laboratory shaker, the long-term reliability of the drive is of paramount importance. The drive moves a platform that keeps the user's sample vessels in constant, uniform, orbital motion. This movement keeps, for example, eukaryotic suspension cells agitated in the culture medium and prevents them from settling in the vessel. This movement is essential for successful growth and, consequently, for successful protein expression. Contamination is one of the greatest safety risks in this application, so the sample chamber must be kept as clean as possible.

In existing laboratory shakers, significant portions of the drive components are often located inside the chamber and are usually covered by an enclosure. According to the observations underlying the invention, the often complex shape and difficult-to-access contours of these components mean that impurities and contaminants in this area of the chamber are difficult or impossible for the user to reach and therefore cannot be effectively removed. Furthermore, with this arrangement, a user or service technician must access the chamber to clean or maintain the drive components, which increases the risk of further contamination. Similarly, working on components inside a sample chamber contaminated with biological samples poses a health risk to service personnel. If the interior of these laboratory shakers needs to be cleaned or even sterilized in the event of potential contamination, the arrangement of the drive components inside the chamber makes the necessary work more difficult.

The present invention is therefore based on the objective of providing a laboratory shaker that has an improved design and is particularly easy to clean.

The invention solves this problem by means of the laboratory shaker according to claim 1 and the method for sterilizing the laboratory shaker according to claim 15. Preferred embodiments of the invention are the subject of the dependent claims and will become apparent from the present description of the invention and the figures.

The chosen design of the sealing elements enables a chamber interior free of drive components and easy to clean. Despite the thermal weak points created by the chamber bottom openings, the sealing elements prevent excessively cool surface temperatures on the chamber bottom wall, which would lead to condensation. The design according to the invention also supports the low overall height of the laboratory shaker, as the chamber interior can be optimally utilized even with large sample vessels. This height is particularly advantageous for shakers used in stacks, as the upper units must also remain ergonomically accessible. Furthermore, optimal height utilization is important for devices intended to achieve higher throughput or optimal protein yield during expression in suspension cells.

The two preferred technical concepts for sealing elements with sliding bearings do without an elastomer part firmly connected between the chamber bottom wall and the connecting element in order to counteract the wear of the elastomer under its high load caused by continuous shaking motion.

Preferably, the sealing element comprises or consists of an elastomeric material, in particular a silicone material, preferably platinum-crosslinked silicone, or a fluororubber.

Preferably, the sealing element is a flat component whose maximum extent in directions parallel to the chamber floor wall is greater than its maximum extent measured perpendicular to the chamber floor wall.

Preferably, the sealing element has a through-channel, particularly a central one, with a through-opening, through which the connecting element passes and against which the connecting element fits tightly, and/or wherein the sealing element is mounted and/or fastened to the connecting element. Preferably, the through-channel is axial, and its wall thickness is greater than the thickness of a radially extending wall of the sealing element.

Preferably, the connecting element has at least one fastening section along its longitudinal axis A, the latter having a radial extension that varies. Preferably, the sealing element, in particular its passage channel, contacts this fastening section and encloses it, in particular in a form-fitting manner.

1. i) vorzugsweise die Gleitlagereinrichtung eine sich parallel zur Kammerbodenwand erstreckende Gleitfläche aufweist; ("axiales Gleitlager") oder alternativ,
2. ii) vorzugsweise die Gleitlagereinrichtung eine sich nicht parallel oder senkrecht zur Kammerbodenwand erstreckende Gleitfläche aufweist, ("radiales Gleitlager")

Preferably, the sealing element is movably mounted on the chamber bottom wall by means of a sliding bearing device, wherein

1. i) preferably the sliding bearing arrangement has a sliding surface extending parallel to the chamber bottom wall; (“axial sliding bearing”) or alternatively,
2. ii) preferably the sliding bearing device has a sliding surface that does not extend parallel or perpendicular to the chamber bottom wall, ("radial sliding bearing")

Preferably, the sealing element is a cap element, particularly a disc-shaped one, that covers the chamber bottom opening, especially during the operation of the drive device. This is particularly useful for the sliding bearing of the sealing element.

Preferably, the connecting element ends below an imaginary plane, which is preferably located in the chamber and runs parallel to the chamber bottom wall at a distance h, measured perpendicular to the chamber bottom wall, wherein h is preferably ≤ 10 cm, preferably h is ≤ 5 cm, preferably h is ≤ 3 cm. This ensures that the The platform can be positioned near the chamber floor, thus optimizing the use of the chamber interior.

The length L of the connecting element is preferably as short as possible and can in particular be L <= 200 mm, preferably 5 <= L <= 120 mm, preferably 8 <= L <= 120 mm.

Preferably, the longer part of the connecting element, measured along its longitudinal axis A, is arranged outside the chamber and below the chamber floor wall. This allows for a short distance between the platform device and the chamber floor wall, and optimizes the use of the chamber interior space.

Preferably, the sealing element is attached to the connecting element and configured to slide along a sliding surface parallel to the chamber bottom wall during the shaking motion. This sliding surface is provided at the chamber wall opening, particularly in the form of a circular ring around it. This concept is referred to as axial sliding bearing. Preferably, the sealing element has a sealing ring section extending parallel to the planar sliding surface and around the chamber wall opening, which is in contact with the sliding surface during sliding.

Preferably, the sealing element is flat and in particular has a sliding plane which runs substantially parallel to the chamber bottom wall, wherein preferably the sealing element anchored to the connecting element is arranged to compensate for inclination deviations between the sliding plane and the flat chamber bottom wall by means of a mobility of the sealing element, in particular by the sealing element having at least one elastically deformable, in particular annular, section or being completely elastically deformable.

Preferably the connecting element and/or the drive component has at least one connecting means, in particular a screw, in particular A long screw that passes through a central cavity or through-channel in the connecting element. Preferably, the connecting element is detachably connected to the drive component, particularly when the bottom opening is sealed by the sealing element. Preferably, the connecting element includes a thread designed to create a screw connection between the connecting element and the drive component, and which is arranged, in particular, concentrically to a central longitudinal axis of the connecting element.

Preferably, a transmission device, which may be one-piece or multi-piece, in particular a support platform, in particular a transmission plate, is arranged on at least one drive component, in particular a gear element, and the at least one connecting element is connected to it. This gear element, in particular a support platform, is in particular arranged outside the chamber.

Preferably, an insulating layer made of a thermally insulating material is arranged below the chamber, adjacent to or adjoining the chamber bottom wall or the heating coil preferably located there. This insulating layer particularly has an opening through which the connecting element passes. The opening is preferably concentric with the chamber bottom opening. Preferably, an insulating element connected to the connecting element is provided, which is movable relative to the insulating layer together with the connecting element and which covers or closes the opening in the axial direction, particularly also during shaking.

Preferably, the laboratory shaker has a heating device, which in particular includes heating coils - preferably on the outside of the chamber - and an electrical control device which is programmed to execute a high-temperature program by which the control device is programmed to heat the chamber interior, sealed by the at least one sealing element, to a predetermined temperature for a predetermined period of time by means of the heating device, wherein the period can be between 1 minute and 12 hours, and the temperature can be between 90°C and 200°C, in particular 180°.

• eine Kammer (2), die mindestens eine Kammerwand aufweist und eine Kammeröffnung (2a) zum Einstellen und Herausnehmen der Probengefäße (130) in einen Innenraum (3) der Kammer,
• eine Heizeinrichtung (190), die zum Beheizen der Kammer vorgesehen ist, die mindestens einen Heizwendel aufweist, der an der Außenseite der mindestens einen Kammerwand angeordnet ist,

dadurch gekennzeichnet, dass

• die mindestens eine Kammerwand mindestens einen ersten Flächenbereich aufweist, in dem die von dem mindestens ein Heizwendel abgegebene Heizleistung größer ist als in einem zweiten Flächenbereich, insbesondere indem der mindestens ein Heizwendel im ersten Bereich mit einer höheren Flächendichte verlegt ist.

The invention also relates to a laboratory device for incubating liquid laboratory samples contained in sample containers (130), in particular an incubation shaker, comprising

• a chamber (2) having at least one chamber wall and a chamber opening (2a) for placing and removing the sample vessels (130) into an interior (3) of the chamber,
• a heating device (190) intended for heating the chamber, comprising at least one heating coil arranged on the outside of at least one chamber wall,

characterized by the fact that

• the at least one chamber wall has at least a first surface area in which the heating power emitted by the at least one heating coil is greater than in a second surface area, in particular by laying the at least one heating coil in the first area with a higher surface density.

The heating power is specified in watts. It can be measured electrically for a section of heating wire. In practice, the area used as the basis for these specifications is typically between 10 and 50 square decimeters. In the incubation chamber, the vertical spacing of parallel wires in the central areas (secondary areas) of the floor, side, back, and ceiling walls is between 3 and 15 cm, particularly between 4 and 10 cm. In the peripheral areas and near openings of the chamber (primary areas), the spacing is preferably smaller than in the central areas.

Preferably, the heat flux density, specified in watts per square meter, is greater in the first area than in the second area.

The heating wire or heating coil is glued to the chamber wall, in particular by means of an adhesive tape, in particular metallic adhesive tape, in particular aluminium adhesive tape.

Preferably, the area coverage by the at least one heating coil, i.e., the area A_H covered by the heating coil on the surface divided by the area unit A, i.e., A_H / A, is greater in the first area than in the second area.

Preferably, the length of the at least one heating coil laid on the surface per unit area, measured in meters per square meter, is greater in the first area than in the second area.

It is preferred to compare the proportion of the length of heating wire per planar area (for example, centrally in the ceiling wall area of the chamber) - as the second area area - with the proportion of the length of heating wire in the chamber wall edge area and/or a chamber wall opening and/or a chamber wall curvature area - as the first area area.

Preferably, the first surface area is located closer to an edge of the chamber wall, an opening in the chamber wall, and/or a curved area, particularly a corner, of the chamber than the second surface area. In these areas, more heat is dissipated to the surroundings compared to the planar surface of the chamber wall, which can be compensated for by the higher heating coil density or higher heating power. As a result, a more homogeneous chamber temperature is achieved, and the risk of condensation on the first surface area is avoided.

Preferably, the laboratory device has an electrical control unit, in particular a data processing unit, and is preferably programmed to detect the temperature of a chamber, in particular the chamber wall, and in particular to adjust the power of the heating device depending on this temperature.

Preferably, the control device is programmed to regulate the temperature of the heating device to a desired, in particular constant, target temperature. Preferably, the control device is programmed to form a heating control loop configured to regulate the temperature of a heating element of the evaporator, measured by a temperature sensor, to a constant target temperature at which a volume of water in contact with the heating element evaporates and thereby extracts heat from the heating element.

The electronic control device is preferably programmed to control at least one function of the lighting device, in particular the duration and/or intensity and/or color and/or depending on sensor signals, in particular the signal of a door sensor of the incubator.

The functions of the control unit are implemented primarily through program code and/or electronic circuits. The control unit may include a microcontroller, a processing unit (CPU) for data processing, or a microprocessor, each of which may be assigned to the data processing unit.

The control unit can be designed as an independently operating component that controls the functions of the lighting device, but in particular does not control one or more functions of the laboratory equipment to which the lighting device is connected or of which the lighting device preferably is a part.

The control unit can also be formed by a control unit that, in addition to the functions of the lighting device, also controls at least one, several, or all functions of the laboratory equipment to which the lighting device is connected or of which the lighting device preferably forms a part. One of the functions of the laboratory equipment is, in particular, the regulation of the temperature in the incubation chamber of the laboratory equipment, or the regulation of the gas composition in the incubation chamber, especially the CO2 concentration. Another function of the laboratory equipment is, in particular, the control of a user interface module of the laboratory equipment that displays information to the user, especially about sensor values. physical or chemical quantities measured in/on the incubation chamber.

• Aufheizen des Kammerinnenraums mittels der Heizeinrichtung für einen vorbestimmten Zeitraum auf eine vorbestimmte Temperatur, wobei der Zeitraum zwischen 1 Minute und 12 Stunden, insbesondere zwischen 20 Minuten und 5 Stunden, betragen kann, und wobei die Temperatur zwischen 90 °C und 140°C , insbesondere bis zu bis zu 180° oder 200°C, betragen kann.

The invention also relates to a method for treating the interior of a laboratory shaker according to one of the preceding claims, comprising a heating device and an electrical control device programmed to execute a high-temperature program, by which the control device is programmed to heat the interior of the chamber, sealed by the at least one sealing element, to a predetermined temperature by means of the heating device for a predetermined period, wherein the period can be between 1 minute and 12 hours, in particular between 20 minutes and 5 hours, and wherein the temperature can be between 90°C and 140°C, up to 180°C or 200°C, wherein the method comprises the step:

• Heating the interior of the chamber by means of the heating device to a predetermined temperature for a predetermined period, wherein the period may be between 1 minute and 12 hours, in particular between 20 minutes and 5 hours, and wherein the temperature may be between 90°C and 140°C, in particular up to 180° or 200°C.

Preferably, the sealing element has at least one eccentric disc and, in particular, a sliding surface with a radial orientation.

Preferably, the sealing element has at least one eccentric disc which has at least one sliding surface with a radial orientation, in particular with an orientation radially outwards.

Preferably, the sealing element has at least one first eccentric disc, which has at least one sliding surface with a radial orientation, in particular with an orientation radially outwards, and preferably the sealing element has at least one second eccentric disc, which has at least one sliding surface with a radial orientation, in particular with an orientation radially outwards. This arrangement is also referred to as a double eccentric disc. The connecting element is preferably rotatable about its longitudinal axis and mounted acentrically in the second eccentric disc, preferably by means of a spherical bearing.

Preferably, the sealing element has a sealing ring section that runs parallel to the sliding surface, which is perpendicular to or at least inclined relative to the chamber bottom wall and runs concentrically to the chamber wall opening, and which is in contact with the sliding surface when sliding.

Preferably, the sealing element is flat and has a main plane that runs substantially parallel to the chamber bottom wall, wherein the sealing element anchored to the connecting element has at least one elastically deformable, in particular annular, section or is completely elastically deformable.

Preferably, the sealing element has magnetic sections whose magnetic attraction pulls the sealing element towards the chamber bottom wall.

Preferably, a radial sliding surface is provided on an annular insert element, in particular a bearing sleeve, which is attached to the chamber wall opening and in particular projects into it.

Preferably, the sealing element has a curved wall section, which is designed in particular as an annular trough, in the center of which a passage channel or opening for the passage of the connecting element is provided.

Preferably, a retaining ring element is provided with which the sealing element is attached to the chamber bottom opening, and which extends in particular with a hollow cylindrical section towards the device space.

The laboratory shaker for shaking laboratory samples is specifically designed for tempering the samples. Such devices are electrically operated and have a power connection. The laboratory shaker tempers the laboratory samples, that is, It maintains the interior of the housing, and thus the laboratory samples stored there, within tolerances by means of temperature control at a setpoint temperature, which is particularly adjustable by the user. This temperature can be above room temperature (ambient temperature), as is the case with a heating cabinet or incubator, or below room temperature, as is the case with a refrigerator or freezer. In a laboratory shaker designed as a climate-controlled laboratory shaker, a climate parameter prevailing inside the housing is preferably also regulated within tolerances. This climate parameter can be the humidity and/or a gas concentration, e.g., a CO2, O2, and/or N2 concentration. Such a climate-controlled laboratory shaker is, for example, a laboratory shaker for shaking laboratory samples, especially those containing live cell cultures, with an incubator function, also referred to as an incubation shaker.

• Temperaturkontrollierbarkeit der Kammer: Beheizung auf maximal 60, 80 °C für die Zellkultur.
• Drehzahlbereich der Schüttelbewegung: (25 - 500, -1000 rpm).
• Gehäuseformat derart, dass eine Aufstellbarkeit im Labor (auf dem Labortisch, unter dem Labortisch, stapelbare Standmodelle) möglich ist.
• Stapelbarkeit des Gehäuses (2 oder 3 oder mehr übereinander).
• Kapazität und Durchsatz: Gefäßtyp, Größe und Kapazität.
• Beladungsart (von vorne oder von oben).
• CO2-Regelung.
• Photosynthetisches Licht.

Typical features of such laboratory shakers may include one or more of the following:

• Temperature controllability of the chamber: Heating to a maximum of 60, 80 °C for cell culture.
• Shaking speed range: (25 - 500, -1000 rpm).
• Housing format such that it can be set up in the laboratory (on the laboratory table, under the laboratory table, stackable floor-standing models).
• Stackability of the housing (2 or 3 or more on top of each other).
• Capacity and throughput: Vessel type, size and capacity.
• Loading method (from the front or from above).
• CO2 regulation.
• Photosynthetic light.

Particularly preferably, the laboratory shaker is configured to carry out a high-temperature sterilization process inside the chamber using a temperature control device and/or a heating device, in which the chamber is kept at a temperature between 150°C and 200°C, preferably at least, for a period of several seconds (e.g., 1, 2, 5, 10, 30 seconds) to several minutes (e.g., up to 1, 2, 3, 5, 10, 30, 60, 120, 240, 480, or 600 minutes). The laboratory shaker is exposed to 180°C without the need to remove the extraction mechanism, preferably including the attached sample platform. Particularly preferably, the laboratory shaker, especially an electronic control device which controls the temperature control and/or heating device, is configured to expose the chamber to a target temperature between 150°C and 200°C, preferably at least 180°C, for a period of more than one hour, particularly for a period of several hours, e.g., within a time interval of 2, 3, 4, 5, 6, 7, 8, 9, or 10 hours. The chamber typically requires a heating period to reach the target temperature and a cooling period to cool back down from the target temperature to a normal operating temperature. In particular, the extraction mechanism is located inside the sealed chamber during the high-temperature cycle. The extraction mechanism and the sample platform are made of a suitably high-temperature resistant material, in particular stainless steel or aluminum, in particular anodized aluminum.

The laboratory shaker preferably has a housing. The housing is preferably an outer housing whose walls are in contact with the environment. The housing door can accordingly be an outer housing door that, in the closed position, borders the environment.

The housing door has, in particular, a hinge mechanism that pivots the housing door to the housing. Such a hinged door is moved between an open and a closed position by rotation. The hinge mechanism can, in particular, be located on the vertically oriented outer edge of a cuboid housing, adjacent to the housing opening, as is typical for the laboratory shaker in normal use. The base plate of a cuboid housing is arranged horizontally in normal use, the side walls of the housing are arranged vertically, and the top plate of the housing is arranged horizontally opposite the base plate.

A data processing unit is preferably part of the electrical control unit that controls the functions of the laboratory shaker and which the laboratory shaker preferably includes. The functions of the control unit are implemented, in particular, by electronic circuits. The control unit can include a microcontroller, a processing unit (CPU) for data processing, and/or a microprocessor, each of which can incorporate the data processing unit. The control unit and/or the data processing unit is preferably configured to carry out a control method, also referred to as control software or control program. Such a control method can define the temporal progression of a shaking motion that can be performed by means of the shaking device. This shaking motion is defined, in particular, by the direction(s) of translational movements and/or the amplitude(s) of successive movement segments that are performed in an x-y plane. This x-y plane is generally parallel to the sample platform and/or a chamber floor. Preferred diameters for a shaking motion performed in an x-y plane are between 0 and 5.08 cm (2 inches) or up to 7.62 cm (3 inches). The shaking device, in particular an orbital drive, is preferably configured for a shaking motion with a maximum diameter between 0 and 5.08 cm or up to 7.62 cm (3 inches). The functions of the laboratory shaker and/or the control device can be described in process steps. They can be implemented as components of the control program, in particular as subprograms of the control program.

Preferably, the laboratory shaker is an incubation shaker. The incubation shaker can then also be operated as a laboratory incubator and is thus a device with which controlled climatic conditions for various biological development and growth processes can be created and maintained. It serves in particular to create and maintain a microclimate with regulated gas, humidity, and/or temperature conditions in the chamber, whereby this treatment can be time-dependent. The incubation shaker can, in particular, include a timer, especially a time switch, a temperature control device designed as a heating and/or cooling unit, and preferably a setting for controlling one of the Chamber supplied exchange gas, an adjustment device for the composition of the gas in the chamber of the incubation shaker, in particular for adjusting the CO ₂ and/or the O ₂ and/or the N ₂ content of the gas and/or an adjustment device for adjusting the humidity in the chamber of the incubation shaker.

The incubation shaker comprises, in particular, the incubator chamber (=chamber), and preferably a control device with at least one control loop, to which at least one temperature control device is assigned as the actuator and at least one temperature sensor as the measuring element. Depending on the embodiment, the humidity can also be controlled, with the relative humidity preferably being measured by a relative humidity sensor (rH sensor) in the chamber, and the relative humidity being, in particular, the input variable of the control loop. For humidifying the chamber, a water-filled tray can be provided in the incubator chamber, which can be heated or cooled to adjust the humidity via evaporation. However, it is more preferred to provide an evaporator device on the outside of the chamber, which actively generates steam as needed and supplies it to the chamber through a steam inlet opening in the chamber wall. This steam supply is preferably controlled by the control device. _CO₂ incubation shakers are used, in particular, for the cultivation of animal or human cells.

The control device can be configured to automatically select a program parameter or a control parameter of the laboratory shaker, in particular the incubation shaker, depending on other data. In an incubator, treatment of at least one cell culture in at least one cell culture container controlled by a control parameter corresponds in particular to climate treatment to which the at least one cell culture is subjected. Possible parameters, in particular program parameters, in particular user parameters, used to influence climate treatment define, in particular, the temperature of the chamber in which the at least one sample is incubated, the relative gas concentration of _O₂ and/or _CO₂ and/or _N₂ in the chamber, the humidity in the chamber, and/or at least one process parameter that determines the process. in particular, it influences or defines the sequence of a multi-step incubation treatment program and/or shaking program.

The temperature control device can be a combined heating/cooling device. Preferably, it is solely a heating device. This can, in particular, generate heat via an electrical resistance wire. Preferably, the resistance wire is attached to the outside of at least one, several, or all of the chamber walls that form the chamber.

Laboratory shakers, or incubation shakers, can have a single chamber or multiple chambers, the atmosphere of which (temperature, relative gas concentration, humidity) can be individually or collectively adjusted. A typical chamber volume ranges from 50 to 400 liters, although smaller chamber sizes, particularly 10 to 49 liters, are available for specific applications (IVF).

The platform assembly is removable, in particular from the interior of the chamber. The platform assembly is mountable to and detachable from at least one drive component, in particular a transmission device, especially a transmission plate, which transmits the shaking motion from the drive device to the platform assembly. For this purpose, at least one connecting element is provided, which detachably connects the platform assembly, in particular a sub-platform of the platform assembly, to the drive component, in particular the transmission device, especially a transmission plate. The at least one connecting element can be designed for tool-free assembly or detachment of this connection; however, assembly with a tool is also preferred. For this purpose, the at least one connecting element can include a screw with a screw head having a suitable contour for positive engagement with the tool, or a screw with a hand-operated rotating head, with a locking device, or with a quick-release clamping device.

The transmission device preferably comprises at least one transmission element, in particular a transmission plate, which is especially a component of the drive device to which the platform device is detachably connected and through which, in particular, the shaking motion is transmitted to the platform device. The transmission element can also be a frame device, in particular a rack device. The transmission device can have several transmission elements that support the transmission of the shaking motion to the platform device.

The platform assembly may include a sub-platform which is connected and/or connectable to the drive component, in particular the transmission plate, by means of at least one connecting means.

The platform assembly can include a support platform for carrying the sample containers to be shaken within the chamber. The support platform can be detachably connected to the sub-platform and can, in particular, be movably mounted on the sub-platform via a rail system.

The platform assembly is always located in the chamber during operation of the laboratory shaker, i.e., during the shaking motion. The platform assembly is preferably fixed or immovable during operation, but in particular, it is detachably connected to the transmission device.

The platform assembly can include a rail system by means of which the support platform can be partially, in particular by 40-95%, or completely extended out of the chamber when the chamber door is open. This allows for convenient loading and unloading of the laboratory shaker. Furthermore, it simplifies the disassembly of the platform assembly, especially the sub-platform, from the transmission system. The rail system comprises, in particular, first rail elements mounted on the sub-platform and second rail elements mounted on the support platform. The second rail elements can be mounted on the first rail elements by means of sliding bearings and/or rolling bearings.

The apparatus space is preferably located below the platform. This allows the apparatus space to be easily separated from the sample space within the chamber, in which the platform and the sample containers are arranged on the platform. The apparatus space can be a space within the chamber. Preferably, the apparatus space is a space outside the chamber, particularly below the chamber. "Below" means "in the direction of gravity," since the laboratory shaker is arranged in its intended operation such that a support platform has a horizontal support area.

The device compartment is also referred to as the drive compartment, since at least one drive component is located there. However, it is also possible that components not belonging to the drive device are located there, such as electronic components, for example, an electronic control unit for the laboratory shaker. These electronic components can include at least one circuit board.

The laboratory shaker has a drawer assembly with at least one, preferably exactly one, drawer element—or two, three, four, or more drawer elements—which is movably mounted in the fixture space and to which the at least one drive component is connected and which, in particular, supports this at least one drive component. During operation of the laboratory shaker, the drawer element is preferably detachably connected to a base of the laboratory shaker by means of fasteners, in particular screws, a locking device, or a quick-release device. Before the drawer element is pulled out of the fixture space, this fixed connection created by the fastener must be released.

The at least one drawer element is movable between a first position, in which the at least one drawer element is arranged in the fixture space, and a second position, in which the at least one drawer element is extended out of the fixture space. Preferably, the drawer assembly is designed for this purpose. The drawer element is arranged so that in the second position it can be pulled out of the fixture space at least 50%, preferably at least 70%, preferably at least 80%, preferably at least 90%, or preferably at least 95%, or preferably completely. In the latter two cases, the extension is referred to as "full extension".

Preferably, the laboratory shaker has a base that supports the remaining components of the laboratory shaker. The base is particularly suitable for supporting at least one additional laboratory shaker—or several—if the laboratory shakers are stackable. The drawer element is preferably movably connected to the base and, in particular, movably mounted on the base by means of a guide device, especially a rail device. The guide device is preferably configured to guide the at least one, preferably exactly one, drawer element relative to the base of the laboratory shaker during a translational extension movement. The guide device can have a first guide element, in particular a rail, arranged on a first side of the drawer element, and can have a second guide element, in particular a rail, arranged on a second side of the drawer element opposite the first side.

The drawer element can have at least one bearing section by which the drawer element is supported on the device base, in particular a bearing section of the device base. The bearing section can be a component of a plain bearing, but can also include a rolling bearing. The bearing section can, in particular, have one or more plastic elements designed to slide on a plain bearing, in particular a sliding surface, of the device base. The plastic element can be a plate or a membrane. Polycarbonate or POM are, for example, suitable plastics for this purpose.

Preferably, the drawer assembly has a rail assembly by means of which the at least one drawer element, in particular by means of a sliding or The rail assembly is movably mounted in the fixture space at the base of the device. The rail assembly comprises, in particular, first rail elements mounted at the base of the device and second rail elements mounted at the drawer element. The second rail elements may be mounted to the first rail elements by means of sliding bearings and/or rolling bearings.

Preferably, the drive device includes a drive unit, in particular an electric motor, which is rigidly connected to the device base, in particular by positive locking, force locking, and/or material locking. In this case, the drive unit is not mounted on the drawer element and is therefore not moved out of the fixture space of the laboratory shaker when the drawer element is moved from the first to the second position.

Preferably, the drive device includes a drive unit, in particular an electric motor, which is rigidly connected to the device base, in particular by positive locking, force locking, and/or material locking. In this case, the drive unit is not mounted on the drawer element and is therefore not moved out of the fixture space of the laboratory shaker when the drawer element is moved from the first to the second position.

The drive device can also include a drive unit, in particular an electric motor, which is rigidly connected to the at least one drawer element, in particular by positive locking, force locking, and/or material locking. In this case, the drive unit is mounted on the drawer element and is therefore also moved relative to the device base, in particular moved out of the device space when the drawer element is moved from the first to the second position.

Preferably, the at least one drive component includes a pulley that is coupled to the drive unit via a belt, which is arranged in particular next to the drawer device.

Preferably, the drive unit is a direct drive. The output shaft of the direct drive is preferably coaxially connected to a drive disc, in particular an eccentric disc, in order to drive it—especially without the use of a gearbox. In this case, the drive unit is preferably arranged on the drawer element. The drive unit can be a flat electric motor whose height is less than its width and/or depth. In particular, the drive unit can be a disc-shaped motor, especially a disc rotor motor.

The drive disc preferably has a transmission element that is arranged eccentrically to the axis of rotation of the drive disc and that, in particular, causes the shaking motion. The transmission element preferably connects the drive disc to the transmission device.

Preferably, the at least one drive component has one or more movable base parts, also referred to as bearing elements or idlers, which are connected in particular to the at least one drawer element and in particular to the transmission device or the transmission plate.

An idler is specifically designed to support the platform mechanism, which is driven by the eccentric disc and performs an orbital motion. The idler does not have an active drive function; rather, it serves to stabilize and support the transmission mechanism while it is driven by the eccentric.

The idler is typically located at a point along the path of the transmission device and helps to stabilize and guide the lateral movement while the platform device performs the shaking motion. It helps to reduce the stress on the transmission element and drive components, thus extending the system's service life.

In summary, the at least one idler supports the transmission device and thus the platform device by ensuring stable guidance along the path of the orbital motion and stabilizing the eccentric movement of the eccentric.

Preferably, the drive device comprises a drive unit configured to provide a drive motion and a gear unit configured to convert the drive motion into the shaking motion, the gear unit comprising at least one gear element. The drive unit and the at least one gear element are drive components, at least one of which is connected to the at least one drawer element. The drive motion is, in particular, a rotational motion of the output shaft of an electric motor. The shaking motion of the platform device is such that all points on the platform device move integrally in a common, planar, orbital path, defined as the superposition of two translations and with an eccentricity.

The transmission unit includes, in particular, those moving components that are located in the kinematic chain between the drive unit and the connecting elements that connect the platform unit to the drive device.

Preferably, the chamber is bounded by a chamber floor wall, which separates the interior of the chamber from the device space preferably provided below the chamber floor wall and which is designed to couple the drive device with the platform device, in particular by having at least one floor opening, in particular several floor openings, preferably two, three or preferably four floor openings.

Preferably, the chamber is bounded by a chamber floor wall. Preferably, the laboratory shaker has at least one connecting element, in particular a coupling rod, by which the platform assembly is detachably connected to the at least one drive component. The connection by this connecting element is preferably positive-locking and/or friction-locking. The at least one connecting element extends through at least one opening in the bottom wall of the chamber when the platform is connected to the at least one drive component. Preferably, several openings are provided in the bottom, each through which exactly one connecting element extends.

Preferably the shaking movement runs parallel to the chamber bottom wall, i.e., in particular horizontally, wherein at least one bottom opening of the chamber bottom wall is preferably dimensioned in such a way that the relative movement of the connecting element and the chamber bottom wall corresponding to the shaking movement is enabled.

Preferably, the at least one bottom opening is sealed by a sealing element arranged between the chamber bottom wall and the connecting element. Preferably, each bottom opening is sealed by a sealing element, which is arranged, in particular, between the chamber bottom wall and the connecting element.

Preferably, the sealing element is connected to the connecting element, but preferably not to the chamber bottom wall, or preferably also to the chamber bottom wall. The connection is preferably force-fit and/or form-fit and/or material-fit.

Preferably, the connecting element and/or a drive component, in particular the transmission device, has at least one connecting means, in particular a screw or long screw, which is guided in particular through a central bore in the connecting element, or a locking or quick-release device. The connecting element is preferably detachably connected to the drive component, in particular the transmission device, by means of the at least one connecting means, particularly while the bottom opening is sealed by the sealing element.

Preferably, the connecting element has a thread that allows for the creation of a screw connection between the connecting element and the transmission device. is set up and is arranged in particular concentrically to a central longitudinal axis of the connecting element.

Preferably, the at least one drive component, in particular a gear element, comprises the transmission device, in particular a transmission plate, for supporting the platform device. The at least one connecting element can be connected to the transmission device.

Preferably, the laboratory shaker has a drawer device with at least one drawer element that is movably mounted in the device space and to which the at least one drive component is connected.

Preferably, the at least one drawer element is movable between a first position, in which the at least one drawer element is arranged in the fixture space, and a second position, in which the at least one drawer element is at least partially extended from the fixture space. In particular, the transmission device is connected to at least one drawer element when it is moved between the first and second positions.

Further preferred embodiments of a laboratory shaker according to the invention can be found in the description of the exemplary embodiments according to the figures. The same reference numerals denote essentially identical components.

• Fig. 1a zeigt eine perspektivische seitlich-frontale Ansicht eines erfindungsgemäßen Laborschüttlers gemäß Ausführungsbeispiel.
• Fig. 1b zeigt den Laborschüttler der Fig. 1a, mit einer abgenommenen Seitenwand und den seitlich der Kammer in einer Elektronikkammer angeordneten Komponenten.
• Fig. 1c zeigt den Laborschüttler der Fig. 1a, mit geöffneter Schwenktüre, wobei die Dichtungselemente, Verbindungselemente, Subplattform und Plattform sowie Probengefäße aus der Kammer entfernt sind und deshalb nicht dargestellt sind.
• Fig. 1d zeigt den Laborschüttler der Fig. 1c, mit abgenommener Frontplatte, hinter der sich der, unterhalb der Kammer gelegene, Antriebsraum befindet.
• Fig. 2a zeigt perspektivisch ein mit einem kappenförmigen Dichtungselement versehenes Verbindungselement, das mit dem Laborschüttler der Fig. 1a bis 1d verwendbar ist.
• Fig. 2b zeigt eine Querschnittsansicht des mit dem kappenförmigen Dichtungselement versehenen Verbindungselements der Fig. 2a, geschnitten durch die zentrale Längsachse A des Verbindungselements.
• Fig. 3 zeigt die mit dem kappenförmigen Dichtungselement versehenen Verbindungselemente der Fig. 2a und Fig. 2b, die auf einer als Übertragungsplatte gebildeten Antriebskomponente des Laborschüttlers der Fig. 1a bis Fig. 1d montiert sind, wobei die Subplattform und Plattformeinrichtung im Kammerinnraum nicht gezeigt sind.
• Fig. 4 zeigt den Laborschüttler der Fig. 2a, mit geöffneter Schwenktüre, in einer geschnittenen Ansicht, bei der der Laborschüttler parallel zu einer Seitenwand und durch die zentralen Längsachsen zweier Verbindungselemente geschnitten dargestellt ist.
• Fig. 5a zeigt eine Querschnittsansicht durch ein Verbindungselement mit Dichtungselement für eine axialer Gleitlagerung, gemäß einer weiteren bevorzugten Ausgestaltung, welche in einem Laborschüttler gemäß Fig. 1a bis 1d verwendbar sind.
• Fig. 5b zeigt eine perspektivische seitlich-frontalen Ansicht des Laborschüttlers gemäß Fig. 1a bis 1d, der mit dem in Fig. 5a gezeigten Verbindungselement mit Dichtungselement versehen ist.
• Fig. 5c zeigt eine weitere Komponentenanordnung des Laborschüttlers gemäß der Fig. 5b, wobei die Kammer mit Kammerbodenwand und der darauf angeordneten Gleitfläche für das kappenförmigen Abdichtelement der Fig. 5a gezeigt ist.
• Fig. 6a zeigt, in einer Aufsicht von oben auf die parallel zur Kammerbodenwand verlaufenden Hauptebene des Dichtungselements, ein Dichtungselement mit radialer Gleitlagerung gemäß einer weiteren bevorzugten Ausführungsform des Dichtungselements, das in einem Laborschüttler gemäß der Figuren 1a bis 1d verwendet werden kann.
• Fig. 6b zeigt, in einer perspektivisch geschnittenen Ansicht, das in dem Laborschüttler gemäß Fig. 1a bis 1d verbaute Dichtungselement mit Verbindungselement gemäß der Figur 6a, mit einigen Komponenten des Laborschüttler.
• Fig. 6c zeigt das Verbindungselement mit Dichtungselement der Fig. 6a, in einer entlang der zentralen Längsachse des Verbindungselements geschnittenen Querschnittsansicht.
• Fig. 7a zeigt eine alternative Ausgestaltung eines Verbindungselements mit Dichtungselement und radialer Gleitlagerung, wobei die Ausgestaltung in Details von der Ausführungsform der Fig. 6a bis Fig. 6c abweicht.
• Fig. 7b zeigt das Verbindungselement mit Dichtungselement der Fig. 7a, in einer entlang der zentralen Längsachse des Verbindungselements geschnittenen Querschnittsansicht.
• Fig. 8a zeigt ein mit einem Faltenbalgdichtungselement versehenes Verbindungselement eines weiteren beispielhaften erfindungsgemäßen Laborschüttlers, das im Laborschüttler der Fig. 1a bis 1d anstelle der Dichtungselemente mit axialer oder radialer Gleitlagerung verwendbar ist, in einer entlang der zentralen Längsachse des Verbindungselements geschnittenen Querschnittsansicht.
• Fig. 8b zeigt, in einer perspektivisch geschnittenen Ansicht, das in dem Laborschüttler gemäß Fig. 1a bis 1d verbaute Faltenbalgdichtungselement der Fig. 8a, mit den Komponenten Isolierscheibe, Verbindungselement, Isolierschicht Faltenbalgdichtungselement, Befestigungsring, Kammerbodenwand, Subplattform und Plattformeinrichtung.

They show:

• Fig. 1a shows a perspective side-frontal view of a laboratory shaker according to the invention and an exemplary embodiment.
• Fig. 1b shows the laboratory shaker of Fig. 1a , with a removed side wall and the components arranged laterally in an electronics chamber.
• Fig. 1c shows the laboratory shaker of Fig. 1a , with the swing door open, with the sealing elements, connecting elements, sub-platform and platform as well as sample vessels removed from the chamber and therefore not shown.
• Fig. 1d shows the laboratory shaker of Fig. 1c , with the front panel removed, behind which the drive chamber, located below the chamber, is situated.
• Fig. 2a The figure shows, in perspective, a connecting element equipped with a cap-shaped sealing element, which is used with the laboratory shaker of the Figs. 1a to 1d usable.
• Fig. 2b shows a cross-sectional view of the connecting element provided with the cap-shaped sealing element of the Fig. 2a , cut through the central longitudinal axis A of the connecting element.
• Fig. 3 shows the connecting elements equipped with the cap-shaped sealing element of the Fig. 2a and Fig. 2b , which is mounted on a drive component of the laboratory shaker formed as a transmission plate Fig. 1a to Fig. 1d are mounted, although the sub-platform and platform equipment inside the chamber are not shown.
• Fig. 4 shows the laboratory shaker of Fig. 2a , with the swing door open, in a cutaway view in which the laboratory shaker is shown cut parallel to a side wall and through the central longitudinal axes of two connecting elements.
• Fig. 5a shows a cross-sectional view through a connecting element with a sealing element for an axial sliding bearing, according to a further preferred embodiment, which is used in a laboratory shaker according to Figs. 1a to 1d usable.
• Fig. 5b shows a perspective side-frontal view of the laboratory shaker according to Figs. 1a to 1d , which is connected to the in Fig. 5a The connecting element shown is equipped with a sealing element.
• Fig. 5c shows another component arrangement of the laboratory shaker according to the Fig. 5b , wherein the chamber with chamber bottom wall and the sliding surface arranged thereon for the cap-shaped sealing element of the Fig. 5a shown.
• Fig. 6a Figure 1 shows, in a top view of the main plane of the sealing element running parallel to the chamber bottom wall, a sealing element with radial sliding bearing according to a further preferred embodiment of the sealing element, which is used in a laboratory shaker according to the Figures 1a to 1d can be used.
• Fig. 6b shows, in a perspective cropped view, the process in the laboratory shaker according to Figs. 1a to 1d Installed sealing element with connecting element according to the Figure 6a , with some components of the laboratory shaker.
• Fig. 6c The connecting element with sealing element is shown. Fig. 6a , in a cross-sectional view cut along the central longitudinal axis of the connecting element.
• Fig. 7a shows an alternative embodiment of a connecting element with a sealing element and radial sliding bearing, the design differing in details from the embodiment of the Figs. 6a to 6c differs.
• Fig. 7b The connecting element with sealing element is shown. Fig. 7a , in a cross-sectional view cut along the central longitudinal axis of the connecting element.
• Fig. 8a Figure 1 shows a connecting element of another exemplary laboratory shaker according to the invention, provided with a bellows sealing element, which is used in the laboratory shaker of the Figs. 1a to 1d can be used instead of the sealing elements with axial or radial sliding bearing, in a cross-sectional view cut along the central longitudinal axis of the connecting element.
• Fig. 8b shows, in a perspective cropped view, the process in the laboratory shaker according to Figs. 1a to 1d installed bellows sealing element of the Fig. 8a , with the components insulating disc, connecting element, insulating layer, bellows sealing element, fastening ring, chamber floor wall, sub-platform and platform device.

Fig. 1a Figure 1 shows a perspective side-frontal view of a laboratory shaker 1 according to the invention. It is a CO2 incubation shaker, particularly a stackable one, which implements a method according to the invention for high-temperature disinfection of the chamber interior. The chamber interior 3 of the laboratory shaker has a capacity of 220 liters (220 liters usable volume), with an overall low overall height of the laboratory shaker. This is made possible in particular by the fact that the sealing elements between the drive chamber 4 (also: device chamber 4) and the chamber interior 3 are designed in a flat construction, so that the height H (dimension in the z-direction, i.e., vertically in the intended use of the laboratory shaker) of the sealing elements is smaller than their width B (x-direction) or length L (y-direction), so that preferably B, L < c, where c can be: 1; 0.75; 0.5; 0.25; 0.2; 0, 1, depending on the design of the sealing element. Different designs of the sealing element are described below:

Particularly preferred is the first preferred embodiment of the sealing element 50 with an axial sliding bearing, which is located in the Figures 1a to 4 as shown in a first variant, and in the Figures 5a to 5c as shown in a second variant, 50'.

In a second preferred embodiment, the sealing element 150 has a radial sliding bearing which is located in the Figures 6a to 6c as shown in a first variant, and according to the Figures 7a and 7b as shown in a second variant, 150'.

In a third preferred embodiment, the sealing element 250 has the form of a flat bellows which does not have a sliding bearing but is firmly connected to the chamber bottom wall 31. This embodiment is described in the Figures 8a and 8b shown.

The laboratory shaker 1 has a housing 19 in which the chamber 2 is arranged. With the swing door 10 closed, the chamber 2 is sealed by means of a door seal 10a and sealing elements 50, 50', etc., such that during operation of the laboratory shaker 1, only a negligible exchange of gases or water vapor occurs between the chamber interior 3 and the environment of the laboratory shaker. The drive 20 of the laboratory shaker is located outside the chamber 2. This allows the chamber interior 3 to be used efficiently; in particular, the drive components are not heated during a high-temperature sterilization process applied to the chamber interior.

In particular, a large part of the drive is located in the drive compartment 4, which is situated below the chamber floor 31. Part of the drive, as well as other components, are located in the electronics compartment 5, which is situated to the side of the chamber. The housing 19 has a side wall 7, another side wall (not shown), a rear wall (not shown), a front panel 6, and a side panel 8. The hinged door 10 can be pivoted upwards from the front wall plane by a pivoting mechanism 15 with a gas spring; the open position of the hinged door is shown in Fig. 1c and 1d shown.

Fig. 1b Figure 1 shows the laboratory shaker 1 with a removed side panel 7 and the components arranged laterally in an electronics chamber 5. These components include the drive 20, here a BLDC motor, the belt 21 driven by it, the power supply components 22 of the drive and the heating device for heating the chamber, with fan 23, and an electronic circuit board 24. which in particular includes the control unit of the laboratory shaker. This unit is specifically programmed to carry out a high-temperature sterilization process applied to the chamber interior 3, according to one aspect of the invention. Also visible is the water evaporator 25, with which water can be evaporated and introduced into the chamber interior.

Fig. 1c Figure 1 shows the laboratory shaker 1 with the swing door 10 open, the sealing elements, connecting elements, sub-platform and platform, as well as sample containers, removed from the chamber and therefore not shown. Visible is the chamber 2, which is formed from integrally connected (high-alloy steel) stainless steel walls 31, 32, (a chamber made of stainless steel is preferred, but aluminum as a chamber material is also preferred) 33, which are connected to each other by curved wall sections. These chamber walls include the chamber bottom wall 31, the side wall 32, another side wall (not shown), a ceiling wall (not shown), and a rear wall 33. The chamber bottom wall 31 has four bottom openings 35. Concentric to each bottom opening, an annular sliding surface element 36 made of stainless steel is mounted concentrically around the bottom opening and on the chamber bottom wall 31 in the chamber interior 3. The sliding surface element 36 has on its upper side the sliding surface 37 arranged (with tolerances) parallel to the chamber bottom wall. This serves for the sliding bearing of a sealing element 50 (in Fig. 1c, d not shown, see Fig. 2a, 2b ), which is arranged for axial sliding support on the sliding surface.

Fig. 1d shows the laboratory shaker of Fig. 1c , with the front panel 6 removed, behind which the drive compartment 4, located below the chamber, is situated. A drawer 40 is arranged in the drive compartment 4, which can be pulled forward (in the y-direction) from the drive compartment 4 for maintenance purposes by means of a rail system 43, which is rigidly connected to a base of the laboratory shaker 1. The drive pulley 41, driven by the belt 21 and equipped with an eccentric coupling 41a, is rotatably mounted on the drawer 40. The drive component, designed as a transmission plate 44 (not visible here) and arranged above (i.e., in the positive z-direction) the drive pulley (eccentric pulley) 41, is driven by means of the drive pulley 41 into a horizontal position. The pivoting movement (rotational movement) is offset. Four movable base parts (bearing elements, idler 42, in particular with a double ball-bearing eccentric shaft, wherein an upper ball bearing is elastically mounted, are mounted on the drawer plate 40, which on the one hand support the transmission plate 44 and whose horizontally movable bearing mechanisms on the other hand accompany the horizontal shaking movement generated by the drive disc 41.

Fig. 2a and 2b Figure 1 shows a connecting element 60 equipped with a cap-shaped sealing element 50, which is usable with the laboratory shaker 1. The sealing element 50 is designed for axial sliding bearing on the horizontal sliding surface 37 of the chamber bottom wall 31, wherein the sliding bearing 71 (see below) of the sealing element 50 is supported in axial direction A (vertically) on the sliding surface 37 and thereby also seals the chamber interior 3 from the drive chamber 4. This seal is particularly tight against the passage of gas, vapor, and liquid water. Although the CO2 concentration and the humidity decrease in the chamber over time, they are readjusted. This decrease in concentration depends in particular on the rotational speed.

The sealing element 50 is a component rotationally symmetrical with respect to the longitudinal axis A, comprising a body 51, 52 formed by injection molding from platinum-cured silicone or another elastomer. This body is formed by a centrally and axially extending through-channel 51, from which a cover wall section 52 extends radially downwards in a cap-like manner. The wall thickness of the through-channel 51 is greater than the wall thickness of the cover wall section 52. The radial dimensions of the cover wall section 52 are such that the bottom opening 35 remains covered and sealed by the sealing element 50 during all intended movements of the connecting element 60. The maximum orbit, i.e., the maximum amplitude of the connecting element 60 in horizontal directions, is 50 mm.

The sealing element 50 has an annular shape at its radially outermost position on its underside (the side of the sealing element pointing in the negative z-direction). A circumferential groove 54 is provided, in which an annular circumferential spring 71 of an annular sliding bearing ring 70 is inserted, designed for positive engagement in the circumferential groove 54. The sliding bearing ring 70 is thus firmly connected to the body 51, 52 of the sealing element. This connection is such that the sliding bearing, formed by the horizontal sliding bearing surface 71 supported on the sliding surface 37 of the chamber bottom wall, achieves a reliable seal between the chamber interior 3 and the drive chamber 4 for the entire service life of the laboratory shaker 1. This effect is made possible in particular by a suitable material pairing of the sliding surface 37 and the sliding bearing ring 70. The sliding surface 37 is polished stainless steel here, but can also be diamond-coated, ceramic, coated with a sliding lacquer, or anodized aluminum, and the sliding bearing ring is made of PTFE here, but can also be made of PEEK or another abrasion-resistant plastic or composite material, for example.

The sealing effect achieved by the sealing element 50 arises in particular from the fact that the sealing element is pressed downwards when the sub-platform of the platform assembly, located inside the chamber, is mounted on the connecting elements 60, with the sub-platform bearing axially against the circumferential projection 66 on the outside of the head section 63. Tests showed that a sufficient sealing effect can also be achieved by the self-weight of the sealing element, i.e., in particular without the contact force.

In the Figures 2a and 2b The unmounted position of the sealing element is shown, in which the sealing element is placed on the head section 63 of the connecting element 60, but is not yet compressed and instead has its undeformed shape with a total height h. This is reduced here to a mounting height H = 0.9 * h when the sealing element 50 is pressed downwards by the mounted sub-platform. During this assembly, the passage channel 51 is pressed downwards by a distance s = h - H until a stop formed by a circumferential projection 53 on the inside of the passage channel 51 abuts the stop formed by a circumferential projection 67 on the outside of the head section 63 of the connecting element. The diameter of the disc-shaped The sealing element 50 has a length of 150 mm and a height h of 35 mm. The length L of the connecting element 60 is 95 mm.

The contact force, approximately 4-8 N, is generated by the axial elasticity of the cover wall section 52 of the sealing element. This contact force could also be achieved by a compression spring (not used here) positioned between the sub-platform and the sealing element – in this case, the body of the sealing element would not necessarily have to be made of an elastomer. A further advantage of the sealing element 50, made of or using elastomer, is that the elastomer can compensate for any deviation between the chamber bottom wall 31 and the sub-platform 44 from the ideally parallel position of the two plates 31 and 44, thus guaranteeing the sealing effect. Such a deviation is typically around 0.5°, but could be compensated for up to approximately 1° to 2°.

The connecting element 60 is designed here as a one-piece coupling rod. It is made of steel (stainless steel). In this embodiment of the laboratory shaker 1, four of these coupling rods 60 support the entire weight of the platform and its load, particularly during shaking operation, and are also subjected to shear forces in horizontal directions due to the shaking motion. The coupling rod therefore has a widened base section 62, which is integrally connected to the shaft 61 above it and the head section 63 extending upwards from it.

The connecting element 60 has a through channel 64 that extends through the entire length of the connecting element 60. At its downward end, the through channel 64 has an inwardly projecting circumferential projection 68, which acts as a stop for the screw head of a screw (75, Fig. 3 ). The screw can be inserted from above into the through-channel 64 and, in particular, into its narrower subsection 64a located in the foot section 62, and can thus be screwed firmly to the drive component designed as a transmission plate. A pin element 69 on the underside of the foot section 62 engages in a receptacle on The upper side of the transmission plate is positively engaged, thus preventing rotation of the connecting element 50 about the longitudinal axis A in the mounted position. This mounting device allows maintenance personnel to easily remove the connecting element by accessing it (through channel 64, screw 75) from the chamber interior 3. After removing the connecting elements 60 (and the attached sealing elements 50), the drawer plate 40 with the drive components mounted on it can be easily pulled forward out of the drive chamber 4 of the laboratory shaker, thus facilitating easy maintenance of the drive.

Fig. 3 shows the connecting elements 60 provided with the cap-shaped sealing element 50 of the Fig. 2a and Fig. 2b , which are mounted on a drive component 44 of the laboratory shaker 1 formed as a transmission plate 44, the sub-platform and platform assembly in the chamber interior not being shown. The coupling between the drive and the platform assembly is effected by connecting the coupling rods 60 on the underside of the transmission plate 44 on the one hand by the screw 75 and associated nut, and on the other hand by connecting the sub-platform ( Fig. 4 The platform assembly is connected to the head section 63 of the coupling rod 60. This connection is achieved by screwing the screw element 80 through its external thread 81 into the internal thread 65 formed in the head section 63, clamping the plate of the sub-platform 44 between the stop projection 66 and the underside of the screw head of the screw element 80. The relatively large diameter of the screw head allows the screws to be easily tightened without tools to achieve the necessary coupling strength. Alternatively, locking screws, particularly those made of stainless steel, can be used, which require a tool for tightening.

On the underside of the chamber floor wall 31, the heating wires 100 of the heating coil 100 are visible. These wires are in thermal contact with the chamber floor wall, and their arrangement is suitable for establishing a uniform temperature in the chamber interior 3. This can be achieved either with or optionally without the use of a fan operating in the chamber interior 3. The heating coils are connected with The heating elements are laid with varying surface densities – this ensures a homogeneous temperature distribution and reduces the risk of condensation. Near the chamber opening and at corners, the outer surface of the chamber has more heating wire per unit area than in planar areas, e.g., centrally in the planar ceiling wall area or the planar rear wall area. The area of the chamber floor wall 31 below the heating coil is thermally insulated by an insulating foam panel 95 (e.g., PU foam). At the locations of the floor openings 35 in the chamber floor wall 31, the insulating foam panel 95 has penetrations 96, which are concentric to the floor openings 35 and have the same diameter. It is possible and preferred that insulating plates (not shown here) are arranged above the transfer plate 44 in the area of the connecting elements 60, which thermally insulate the openings 96 of the insulating foam plate 95 in the axial direction even during the shaking operation of the platform, while the transfer plate 44 with the insulating plates moves relative to the insulating foam plate 95.

Fig. 4 shows the laboratory shaker 1, with open swing door 10, in a cut view, in which the laboratory shaker is shown cut parallel to a side wall and through the central longitudinal axes of two connecting elements 50.

Fig. 120 shows in particular the platform assembly 120, which is supported by the connecting elements 60 mounted on the transmission plate 44 of the drive. The sub-platform 121 is clamped between the mounting screws 80 and the coupling rods 60.

The platform assembly 120 includes the sub-platform 121, an essentially plate-like component that extends parallel to the chamber floor wall 31 inside the chamber. The platform assembly has a rail-based extension mechanism (not shown) by means of which the main platform 122, which is mounted on the sub-platform 121 by rails, can be pulled forward (in the y-direction) out of the chamber interior when the pivot door 10 is open. For this purpose, the pivot lever 124 is first tilted forward by 90°, which releases a locking mechanism (not shown) of the main platform 122 on the sub-platform and makes the main platform movable along the y-axis. A cover plate part 123 is removablely mounted on the main platform 122. The cover plate part 123 is in the locked position of the main platform (in Fig. 4 (shown) attached to the main platform 122. Holders for holding eight Erlenmeyer flasks 130 are mounted on the cover plate section 123. Alternative cover plate sections with holders for other sample vessels can be used optionally.

Figs. 5a to 5c Figure 1 shows a connecting element 60' with a sealing element 50' for an axial sliding bearing, according to a further preferred embodiment, which is used in a laboratory shaker 1 instead of the connecting element 60 with connecting element 50. The sealing element 50' can be made of the same material as the sealing element 50. The connecting element 60' is, even if it is in the Figure 5a shown without a cavity, is essentially constructed like the connecting element 60, namely as a hollow coupling rod with an internal thread in the head section of the connecting element 60', into which the screw element 80 is screwed for mounting the connecting element 60' on the chamber floor wall 31, as already described above.

The alternative sealing element 50' is also designed for axial sliding bearing. For this purpose, it has, analogous to the sealing element 50, a sliding ring (PTFE) 70' on its underside, which is manufactured analogously to the sliding ring 70 and which, during shaking operation, slides along the sliding surface 37 of the sliding element 36 on the upper side of the chamber bottom wall 31. The elastomeric body of the sealing element 50' has a central channel section 51' through which the head section of the connecting element 60' passes, to which the sealing element 50' is attached. The radially extending cover wall section 52' is integrally connected to the channel section 51'. An elastic, upwardly extending, and radially inwardly curved wall section 55', serving as a spring element, is formed on its upper side and radially outer end (the body 51', 52', 55' is an injection-molded part). In the assembled state of sub-platform 121 (in Figs. 5a to 5c (not shown) the underside of the sub-platform 121 elastically presses the wall section 55' downwards with a contact force of between 4 and 8 N. This sufficiently seals the bottom opening 35 (liquid-tight), in particular sufficiently against an unwanted Heat exchange between chamber interior 3 and drive chamber 4 is sealed, as is also the case with the sealing element 50.

Figures 6a to 6d show a sealing element 150 with radial sliding bearing according to a further preferred embodiment of the sealing element, which is used in a laboratory shaker 1 according to the Figures 1a to 1d The sealing element 150 is used instead of the sealing element 50 (or 50') with axial sliding bearing. The sealing element 150 has a flat design with a height-to-diameter ratio of c=0.19.

The sealing element 150 is disc-shaped and has the form of a flat cylinder. The sealing element 150 has a first eccentric disc 151, which forms the outer body of the sealing element 150. The sealing element 150 also has a second eccentric disc 152, which forms an inner body of the sealing element 150. In this sense, the sealing element 150 forms a double eccentric disc. The first eccentric disc 151 has a cylindrical outer surface 151a, which serves as a radial sliding surface. The eccentric disc 151 and its sliding surface 151a are dimensioned such that they are fitted for sliding bearing on the inner cylindrical surface 193a of the sliding sleeve 193 and within its inner diameter. The sliding sleeve 193 is itself firmly inserted into the fastening ring 190, which is inserted into the bottom opening 35 of the chamber bottom wall 31 and screwed to it, with a flat sealing ring being inserted between the flange section of the fastening ring 190 and the chamber bottom wall 31 for sealing purposes.

The second eccentric disc 152 is arranged acentrically, i.e., horizontally (in the xy-plane) offset from the center of the first eccentric disc 151. The second eccentric disc 152 is axially rotatable in a cylindrical recess 151b of the first eccentric disc 151 (which is offset acentrically from the center of the first eccentric disc 151). For this purpose, the cylindrical second eccentric disc 152 has a cylindrical radial outer surface that fits into the recess on the inner side 151b of the cylinder of the first eccentric disc 151 for radial sliding bearing.

The second eccentric disc 152 also has a substantially cylindrical recess 154, which is arranged eccentrically, i.e., horizontally (in the x-y plane) offset from the center of the second eccentric disc 151. A spherical bearing 153 is inserted into the recess 154, in which the two-part coupling rod 160, or rather its upper part 160b, is mounted. The spherical bearing 153 allows, on the one hand, axial rotation of the coupling rod 160 within the recess 154, and on the other hand, also a precession movement at small angles to the longitudinal axis A of the coupling rod 160. This achieves a tolerance for deviations from the ideal position of the coupling rod relative to the chamber bottom wall.

During the shaking motion, the sealing element 150, designed as a double eccentric disc, allows the coupling rod, mounted in the spherical bearing 154, to follow every movement imparted by the drive eccentric disc 41 within the bottom opening 35 of the chamber bottom wall 31. This is achieved by the coupling rod 160, mounted eccentrically in the second eccentric disc 152, causing the rotation and movement of the second eccentric disc 152, whose movement then causes the rotation of the first eccentric disc 151. During this shaking motion, the bottom opening 35 remains reliably sealed by the sealing element 150.

The two-part coupling rod 160 has a through channel 164 over the entire length of the coupling rod 160, as shown in Fig. 6b The lower part, analogous to the coupling rod 60, has a lower section with a smaller inner diameter 164a. This section can accommodate the fastening screw (not shown) with which the lower part 160a of the coupling rod 160 is screwed onto the transmission plate 44. An internal thread 165 is provided in the upper section of the lower part 160a of the coupling rod 160. To fasten the sub-platform 121 to the coupling rod 160, a long screw 180 is inserted from above through the receiving bore 121a of the sub-platform 121 into the through-channel 164, and the external thread 181 is screwed into the internal thread 165. This also firmly connects the upper part 160b of the coupling rod 160 to the lower part 160a. The two-part design of the coupling rod 160 simplifies the assembly of the sealing element 150.

Fig. 7a shows an alternative embodiment of a connecting element 160' with sealing element 150' and radial sliding bearing, wherein the embodiment differs in details from the embodiment of the Figs. 6a to 6c differs. The sealing element 150' is also designed as a double eccentric disc. The operating principle is the same:

The sealing element 150' is disc-shaped and approximately resembles a flat cylinder. The sealing element 150' has a first eccentric disc 151', which forms the outer body of the sealing element 150'. The sealing element 150' also has a second eccentric disc 152', which forms the inner body of the sealing element 150'. In this sense, the sealing element 150' forms a double eccentric disc. The first eccentric disc 151' has a cylindrical outer surface 151a', which serves as a radial sliding surface. The first eccentric disc 151' and its sliding surface 151a' are dimensioned such that they fit into the inner cylindrical surface 193a' of the sliding sleeve 193' and into its inner diameter for sliding bearing. The sliding sleeve 193` is itself firmly inserted into the fastening ring 190`, which is inserted into the bottom opening 35 of the chamber bottom wall 31 and screwed to it, with a flat sealing ring 191' being inserted between the flange section of the fastening ring 190' and the chamber bottom wall 31 for sealing purposes.

The second eccentric disc 152' is arranged eccentrically, i.e., horizontally (in the x-y plane) offset from the center of the first eccentric disc 151'. The second eccentric disc 152' is arranged to rotate axially in a cylindrical recess 151b' of the first eccentric disc 151' (which is offset eccentrically from the center of the first eccentric disc 151'). For this purpose, the cylindrical second eccentric disc 152' has a cylindrical radial outer surface that fits into the recess on the inner side of the cylinder of the first eccentric disc 151' for radial sliding bearing.

The second eccentric disc 152 also has an essentially cylindrical recess 154', which is offset acentrically, i.e. horizontally (in the xy-plane) to the The center of the second eccentric disc 151 is located. A spherical bearing 153 is inserted into the recess 154', in which the two-part coupling rod 160', or rather its upper part 160b', is mounted. The spherical bearing 153' allows, on the one hand, axial rotation of the coupling rod 160' within the recess 154', and on the other hand, also a precession movement at small angles to the longitudinal axis A of the coupling rod 160'. This achieves a tolerance for deviations from the ideal position of the coupling rod relative to the chamber floor wall.

The coupling rod 160' has a through channel 164' that extends through the length of the lower part 160a' of the coupling rod 160'. The upper part 160b' of the coupling rod 160' does not have a through channel but is a solid component. It has an external thread in its lower section and a hexagonal outer contour [or alternative fastening method] in its upper section, so that the upper part can be screwed onto the internal thread 165' of the lower part 160a' of the coupling rod 160' using an Allen key. The upper part 160b' also has a bore 182' with an internal thread, so that the sub-platform 121 can be screwed onto the upper part 160b' using a screw (not shown).

Fig. 8a Figure 1 shows a connecting element 260 equipped with a bellows sealing element 250, which can be used in the laboratory shaker 1 instead of the sealing elements with axial or radial sliding bearing, as an alternative to the one shown in the Figures 1a to 7b The embodiments of the laboratory shaker shown, with axial or radial sliding bearing, are shown in a cross-sectional view cut along the central longitudinal axis of the connecting element.

The bellows sealing element 250 does not have a sliding bearing on the chamber floor wall 31; rather, the radially outer edge region of the bellows sealing element 250 is firmly connected to the chamber floor by means of the retaining ring 290. The central section of the bellows sealing element 250 is a channel section 251 through which, in the assembled state, the connecting element 260, a hollow coupling rod, extends. The channel section 251 has a thicker The wall section 252 of the bellows sealing element serves to reliably fasten the bellows sealing element to the coupling rod 260. The coupling rod has a radial engagement groove 261 on its outer side, into which an annular projecting section 253 of the channel section 251 engages in a form-fitting manner.

The wall section 252 of the bellows sealing element 250, extending radially outwards from the channel section 251, reliably seals the bottom opening 35 of the chamber floor wall 31. Since the bellows sealing element 250, or at least the wall section 252, is made of an elastomer, in particular a platinum-cured silicone, the sealing element follows every horizontal shaking movement exerted by the coupling rod 260, with maximum orbital amplitudes of 50 mm. The wall section 252, which extends downwards through the chamber floor wall 31, proves advantageous in several respects. Firstly, its downward orientation results in a flat design for the bellows sealing element 250. Secondly, the shape of the wall section 252 ensures the long-term load-bearing capacity of the elastomeric material of the wall section 252. The wall section here has the form of an annular trough, which is arranged around the connecting element 260 and (in the assembled state) extends radially outwards from the channel section 251 through the bottom opening 35 of the chamber floor wall 31 downwards, and then axially upwards again in the radially outer region of the wall section. The outermost edge 254 of the bellows sealing element 250 is placed from above around a radially outwardly projecting flange section 291 of the sleeve-shaped retaining ring 290, so that the outermost section of the bellows sealing element 250 can be clamped between the chamber floor wall 31 and the flange section 291 of the retaining ring 290. The force-fit fastening of the retaining ring 290 in the bottom opening 35 of the chamber floor wall 31 also reliably secures the edge region 254 of the bellows sealing element 250.

The hollow coupling rod 260 is screwed to the transmission plate 44 by a long screw 281, which extends completely through the coupling rod. The head of the long screw is fixedly connected to a rotary knob 280, which allows for convenient, tool-free tightening of the long screw 281 to the The transfer plate 44 serves this purpose. The sub-platform 121 is clamped between a stop 262 of the head area of the coupling rod 260 and the underside of the rotary wheel 280 and thus reliably fastened.

Claims (15)

Laboratory shaker (1) for shaking biological samples stored in sample containers (130), comprising a temperature-controlled chamber (2) which has a lockable access opening (2a) and a chamber floor wall (31), a platform device (120) on which the sample vessels (130) can be stored, which can be shaken by means of a shaking movement and which is arranged in the chamber (2) parallel to the chamber floor wall (31), a drive device (20, 21, 41, 42, 44) designed to perform the shaking movement, which has at least one drive component (44), and characterized by that the laboratory shaker has a device space (4) arranged outside the chamber (2) in which the at least one drive component is arranged, that the chamber floor wall (31) has at least one floor opening (35) which is closed by a sealing element (50; 50';150;150'; 250), and that the laboratory shaker has at least one connecting element (60; 60';160;160'; 260) that connects the at least one drive component (44) to the platform device (120) and extends through the at least one bottom opening (35) and the sealing element (50; 50';150;150'; 250). Laboratory shaker according to claim 1, wherein the sealing element (50; 50'; 250) comprises or consists of an elastomeric material, in particular a silicone material, preferably platinum-crosslinked silicone. Laboratory shaker according to claim 1 or 2, wherein the sealing element (50; 50'; 150; 150'; 250) is a flat component whose maximum extent in directions parallel to the chamber bottom wall (31) is greater than its maximum extent measured perpendicular to the chamber bottom wall (31). Laboratory shaker according to one of the preceding claims, wherein the sealing element (50; 50'; 150; 150'; 250) has a through-channel (51; 51'; 154; 251), in particular with a through-opening (51a), through which the connecting element passes and on which the connecting element in particular bears tightly, and/or wherein the sealing element is mounted and/or attached to the connecting element. Laboratory shaker according to claim 4, wherein the passage opening is formed in an axial passage channel (51; 51'; 154; 251) whose wall thickness is greater than the thickness of a radially extending wall (52; 52'; 252) of the sealing element. Laboratory shaker according to one of the preceding claims or claim 5, wherein the connecting element has at least one fastening section (63; 261) along its longitudinal axis (A) with a radial extension that varies in particular, and the sealing element, in particular its through-channel (51; 251), contacts this fastening section, in particular enclosing it in a form-fitting manner. Laboratory shaker according to one of the preceding claims, wherein the sealing element (50; 50';150;150') is movably mounted on the chamber bottom wall (31) by means of a sliding bearing device, wherein iii) preferably the sliding bearing device has a sliding surface (37) extending parallel to the chamber bottom wall; (“axial sliding bearing”) or alternatively, iv) preferably the sliding bearing device has a sliding surface (193a; 193a') which does not extend parallel or perpendicular to the chamber bottom wall, (“radial sliding bearing”). Laboratory shaker according to one of the preceding claims, wherein the sealing element (50; 50') is a cap element, in particular a disc-shaped one, which covers the bottom opening (35), in particular during the operation of the drive device. Laboratory shaker according to one of the preceding claims, wherein the connecting element terminates below an imaginary plane which is preferably located in the chamber and which runs parallel to the chamber bottom wall at a distance d, wherein preferably d <= 10 cm, preferably d <= 5 cm, preferably d <= 3 cm. Laboratory shaker according to one of the preceding claims, wherein the longer part of the connecting element, measured along its longitudinal axis, is arranged outside the chamber (2) and below the chamber bottom wall (31). Laboratory shaker according to one of the preceding claims, wherein the sealing element is attached to the connecting element and is arranged to slide along a sliding surface (37) provided parallel to the chamber bottom wall during the shaking movement, which is provided at the chamber bottom opening (35), in particular in a circular ring shape around it. Laboratory shaker according to claim 11, wherein the sealing element has a sealing ring section (70; 70') extending parallel to the planar sliding surface and around the chamber wall opening, which is in contact with the sliding surface (37) when sliding. Laboratory shaker according to one of the preceding claims, wherein the sealing element is flat and has a sliding plane which is substantially parallel to the chamber bottom wall, wherein the sealing element anchored to the connecting element is arranged to compensate for inclination deviations between the sliding plane and the flat chamber bottom wall by means of a mobility of the sealing element, in particular by the sealing element having at least one elastically deformable, in particular annular, section or being completely elastically deformable. Laboratory shaker according to one of the preceding claims, wherein a layer (95) of a thermally insulating material is arranged below the chamber, adjacent to the chamber bottom wall, which has an insulating material opening (96) through which the connecting element passes,
wherein preferably an insulating element (98) connected to the connecting element is provided, which together with the connecting element is movable relative to the layer and which closes the insulating material opening (96), in particular also during the shaking movement. A method for treating the interior of a laboratory shaker according to one of the preceding claims, comprising a heating device (100) and an electrical control device (24) programmed to execute a high-temperature program, whereby the control device is programmed to heat the interior of the chamber, sealed by the at least one sealing element, to a predetermined temperature by means of the heating device for a predetermined period of time, wherein the period may be between 1 minute and 12 hours, and wherein the temperature may be between 90 °C and 140 °C, the method comprising the step: • Heating the chamber interior by means of the heating device to a predetermined temperature for a predetermined period, wherein the period The duration can range from 1 minute to 12 hours, and the temperature can range from 90°C to 140°C.