Classifications

• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
  • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
  • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
  • A23B2/00—Preservation of foods or foodstuffs, in general
  • A23B2/70—Preservation of foods or foodstuffs, in general by treatment with chemicals
  • A23B2/725—Preservation of foods or foodstuffs, in general by treatment with chemicals in the form of liquids or solids
  • A23B2/729—Organic compounds; Microorganisms; Enzymes
  • A23B2/742—Organic compounds containing oxygen
  • A23B2/754—Organic compounds containing oxygen containing carboxyl groups
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23K—FODDER
  • A23K20/00—Accessory food factors for animal feeding-stuffs
  • A23K20/10—Organic substances
  • A23K20/105—Aliphatic or alicyclic compounds
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23K—FODDER
  • A23K20/00—Accessory food factors for animal feeding-stuffs
  • A23K20/10—Organic substances
  • A23K20/111—Aromatic compounds
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23K—FODDER
  • A23K20/00—Accessory food factors for animal feeding-stuffs
  • A23K20/10—Organic substances
  • A23K20/158—Fatty acids; Fats; Products containing oils or fats
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23K—FODDER
  • A23K30/00—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23K—FODDER
  • A23K50/00—Feeding-stuffs specially adapted for particular animals
  • A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23K—FODDER
  • A23K50/00—Feeding-stuffs specially adapted for particular animals
  • A23K50/20—Feeding-stuffs specially adapted for particular animals for horses
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23K—FODDER
  • A23K50/00—Feeding-stuffs specially adapted for particular animals
  • A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23K—FODDER
  • A23K50/00—Feeding-stuffs specially adapted for particular animals
  • A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23K—FODDER
  • A23K50/00—Feeding-stuffs specially adapted for particular animals
  • A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
  • A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
  • A61P31/04—Antibacterial agents

Definitions

• temperature conditions may be chosen to disfavor microbial growth.
• products subjected to a risk of microbial spoilage may be kept frozen or at least may be stored at low temperatures, e.g. below 10 °C.
• Further options to modulate or control microbial growth include the removal of water e.g. by lyophilization, smoking, salting, curing, sugaring or pickling. In some preservation processes, an acidic pH environment is established to disfavor the growth of unfavorable and potentially pathogenic microorganisms.
• acidifier products including organic acids may be used to maintain food and feed hygiene and prevent spoilage by microorganisms such as bacteria or molds.
• Organic acids e.g. formic acid, propionic acid or acetic acid as described in EP 2 642 874 may be used to control microbial growth in foodstuff, food or feed with the aim to minimize the risk for foodborne diseases.
• acidifier products may be added pre- and/or post-production of finished feed.
• acidifier products may be used to achieve a reduced growth of potentially pathogenic microorganisms in the gastrointestinal tract, but also to improve feed conversion rate and weight gain upon ingestion.
• benzoic acid and/or at least one salt thereof in combination with at least one additional organic acid and/or at least one salt thereof, provides enhanced antimicrobial effect.
• the present invention provides a composition for modulating growth of microorganisms, comprising a) benzoic acid and/or at least one salt thereof, and b) at least one additional organic acid and/or at least one salt thereof.
• the present invention also provides use of the above composition for modulating growth of microorganisms, and a method by using the composition for modulating growth of microorganisms.
• the present invention further provides use of at least one additional organic acid and/or at least one salt thereof in the composition as defined herein for boosting the effect of benzoic acid and/or at least one salt thereof in modulating growth of microorganisms.
• the at least one salt of benzoic acid may be selected from the group consisting of sodium benzoate, magnesium benzoate, manganese benzoate, potassium hypophosphite, aluminium benzoate, calcium benzoate and ferric benzoate, preferably sodium benzoate, magnesium benzoate, manganese benzoate and potassium benzoate, and more preferably sodium benzoate.
• examples of the short monocarboxylic acids having between 1 and 6 carbon atom(s) are formic acid, acetic acid, propionic acid, butyric acid, isobutyric acid, 3-methylbutyric acid, 2-methylbutyric acid, 2-ethylbutyric acid, valeric acid and hexanoic acid.
• Examples of the saturated dicarboxylic acids are adipic acid and succinic acid.
• An example of the unsaturated dicarboxylic acid is fumaric acid.
• examples of the unsaturated carboxylic acids are sorbic acid and oleic acid.
• Examples of the saturated carboxylic acids are stearic acid, octanoic acid (also referred to as caprylic acid), decanoic acid (also referred to as capric acid) and dodecanoic acid (also referred to as lauric acid).
• Examples of the hydroxycarboxylic acids are lactic acid, malic acid (D-, or L-, or D/L-malic acid), citric acid and tartaric acid.
• An example of the aromatic carboxylic acids is cinnamic acid.
• An example of the keto carboxylic acid is pyruvic acid.
• the at least one additional organic acid is selected from the group consisting of formic acid, acetic acid, propionic acid, butyric acid, isobutyric acid, 3-methylbutyric acid, 2-metylbutyric acid, 2-ethylbutyric acid, valeric acid, hexanoic acid, adipic acid, succinic acid, fumaric acid, sorbic acid, oleic acid, stearic acid, octanoic (caprylic) acid, decanoic (capric) acid, dodecanoic (lauric) acid, lactic acid, malic acid, citric acid, tartaric acid, cinnamic acid, pyruvic acid, gluconic acid, suberic acid, malonic acid, tannic acid, caffeic acid, ellagic acid, perillic acid and gallic acid.
• formic acid acetic acid, propionic acid, butyric acid, isobutyric acid, 3-methylbutyric acid
• the at least one additional organic acid is selected from formic acid, acetic acid, propionic acid, butyric acid, isobutyric acid, 3-methylbutyric acid, 2-metylbutyric acid, 2-ethylbutyric acid, valeric acid, hexanoic acid, adipic acid, succinic acid, fumaric acid, sorbic acid, oleic acid, stearic acid, octanoic (caprylic) acid, decanoic (capric) acid, dodecanoic (lauric) acid, lactic acid, malic acid, citric acid, tartaric acid, cinnamic acid and pyruvic acid, or at least one salt thereof.
• the at least one additional organic acid is selected from formic acid, acetic acid, propionic acid, fumaric acid and succinic acid.
• the most preferably, the at least one additional organic acid is selected from fumaric acid and succinic acid.
• the at least one salt of the additional organic acid may be any one of metal salts such as potassium, sodium or calcium salts, and ammonium salts.
• the salts of the organic acid include but are not limited to ammonium formate, potassium diformate, sodium diacetate, calcium acetate, ammonium propionate, sodium propionate, calcium propionate, calcium lactate, potassium sorbate, sodium formate, calcium formate, sodium butyrate, sodium sorbate, potassium citrate, sodium citrate and calcium citrate.
• the composition according to the invention may comprise benzoic acid and/or at least one salt thereof in combination with more than one additional organic acid and/or at least one salt thereof.
• such a composition may comprise benzoic acid and/or at least one salt thereof in combination with two, three, four, five or even more additional organic acids.
• the composition comprises benzoic acid and/or a salt of benzoic acid and at least three additional organic acids, especially formic acid, acetic acid and propionic acid, wherein the salt of benzoic acid is selected from the group consisting of sodium benzoate, manganese benzoate, magnesium benzoate and potassium benzoate, preferably sodium benzoate or manganese benzoate, more preferably sodium benzoate.
• the composition according to the invention comprises benzoic acid and/or a salt of benzoic acid and at least four additional organic acids, especially formic acid, acetic acid, propionic acid and fumaric acid, wherein the salt of benzoic acid is selected from the group consisting of sodium benzoate, manganese benzoate, magnesium benzoate and potassium benzoate, preferably sodium benzoate or manganese benzoate, more preferably sodium benzoate.
• the composition according to the invention comprises benzoic acid and/or a salt of benzoic acid and at least five additional organic acids, especially formic acid, acetic acid, propionic acid, fumaric acid and succinic acid, wherein the salt of benzoic acid is selected from the group consisting of sodium benzoate, manganese benzoate, magnesium benzoate and potassium benzoate, preferably sodium benzoate or manganese benzoate, more preferably sodium benzoate.
• composition of the present application may be formulated in the form of a feed additive (premix) or a food additive or a feed or a food for administering to animals.
• a composition may further comprise components, such as one or more of the following: Fat- or water-soluble vitamin(s), trace- and/or macro-mineral(s), (proteinogenic) amino acid(s), organic and/or inorganic adsorbant(s), polyunsaturated fatty acid(s), antimicrobial polypeptide(s), pre- and/or pro-biotic(s), flavoring and/or coloring agent(s), microorganism(s) and/or enzyme(s) to facilitate better nutrient availability and/or to mitigate undesired effects) of feed/food contaminants).
• compositions according to the invention may be provided in a form wherein one or more or all of the components is/are provided in solid form (e.g. salt, powder, granulate, pellet etc.) or in liquid form (e.g. aqueous, gel, viscous). It is also considered that the composition may be provided in a manner, wherein one component (e.g. one or more organic acid(s)) is provided in liquid form and a second component is provided in solid form (e.g. benzoic acid), and the composition is formed by combining the two components in a mixture or in a separate form.
• solid form e.g. salt, powder, granulate, pellet etc.
• liquid form e.g. aqueous, gel, viscous
• one component e.g. one or more organic acid(s)
• a second component is provided in solid form (e.g. benzoic acid)
• said composition may be provided in a particular manner, wherein the molar ratio of the at least one additional organic acid and/or the at least one salt thereof to benzoic acid and/or the at least one salt thereof is from 0.001 :1 to 500:1 , preferably from 0.005:1 to 300:1 , more preferably from 0.01 :1 to 200:1 , such as from 0.01 :1 to 0.15:1 , from 0.02:1 to 0.1 :1 , from 0.04:1 to 100:1 , from 0.05:1 to 100:1 , , from 0.1 :1 to 100:1 , from 1 :1 to 100:1 , from 10:1 to 80:1 and from 20:1 to 40:1.
• the at least one additional organic acid and/or the at least one salt thereof may be provided at any concentration from 0.2 mmol to 100 mmol such as 0.2. 0.4, 0.8. 1.5, 3.1 , 6.25, 12.5, 25.0, 50.0 and 100 mmol, in combination with benzoic acid and/or at least one salt thereof at any concentration from 0.2 mmol to 20 mmol such as 0.3, 0.6, 1 .3, 2.5, 5.0, 10 and 20 mmol, per 1 Kg of the composition in solid form or per 1 L of the composition in liquid form.
• the molar ratio of the at least one additional organic acid and/or the at least one salt thereof to benzoic acid and/or the at least one salt thereof is in the range of from 0.001 :1 to 0.15:1.
• composition of the present invention can be used for modulating growth of microorganisms, i.e., inhibiting the growth of pathogenic microorganisms and/or enhancing the growth of beneficial (i.e. probiotic) microorganisms.
• the microorganisms may be any one or more of the genera of Butiauxella, Citrobacter, Cronobacter, Enterobacter, Escherichia, Edwardsiella, Klebsiella, Phytobacter, Plesiomonas, Pseudoescherichia, Raoultella, Salmonella, Shigella, Proteus, Yersinia, Vibrio, Aeromonas, Clostridium, Pseudomonas, Staphylococcus, Pasteurella, Brachyspira, Campylobacter, Listeria, Streptococcus, Haemophilus, Brucella, Moritella, Tenacibaculum, Lactobacillus, Bifidobacterium, Saccharomyces, Bacillus, Pediococcus, Enterococcus, Streptococcus, Propionibacterium, Pseudomonas and Citrobacter.
• the microorganisms in the present invention may be any one or more of Escherichia coll, Salmonella enterica, Shigella sonnei, Clostridium perfringens, Lactobacillus reuteri, Enterococcus faecium and Bacillus subtilis.
• the pathogenic microorganisms in the present invention may be at least one of the genera of Buttiauxella, Citrobacter, Cronobacter, Enterobacter, Escherichia, Edwardsiella, Klebsiella, Phytobacter, Plesiomonas, Pseudoescherichia, Raoultella, Salmonella, Shigella, Proteus, Yersinia, Vibrio, Aeromonas, Clostridium, Pseudomonas, Staphylococcus, Pasteurella, Brachyspira, Campylobacter, Listeria, Streptococcus, Haemophilus, Brucella, Moritella and Tenacibaculum, in particular may be selected from the group consisting of Escherichia coli, Salmonella enterica, Shigella sonnei and Clostridium perfringens.
• the beneficial microorganisms in the present invention may be at least one microorganism selected from the genera of Lactobacillus, Bifidobacterium, Saccharomyces, Bacillus, Pediococcus, Enterococcus, Streptococcus, Propionibacterium, Pseudomonas and Citrobacter, in particular may be selected from the group consisting of Lactobacillus reuteri, Enterococcus faecium and Bacillus subtilis.
• the composition of the present invention was capable of modulating growth of microorganisms in a synergistic model.
• the term “in a synergistic model” means that, in a test of modulating growth of microorganisms, for example, according to the example 1 of the present invention, the composition of the present invention provides observed value (“O”) beyond the expected value (“E”) resulting from the individual ingredient of the composition, i.e, benzoic acid and/or salt thereof and the additional organic acid and/or salt thereof.
• the expected value (“E”) can be calculated according to the formula developed by Colby etal. (1967. Weeds 15(1): 20-22), which is incorporated herein as reference.
• the expression “observed value (“O”) beyond the expected value (“E”)” means that the result of the observed value minus the expected value, i.e., E - O, is bigger than zero (0), preferably no less than 1 , 2, 3, 5, 8 10, and more preferably no less than 10.
• the present invention provides use of the composition as described herein for modulating growth of microorganisms, i.e., inhibiting the growth of pathogenic microorganisms and/or enhancing the growth of beneficial (i.e. probiotic) microorganisms.
• the present invention provides use of the composition as described herein for the treatment, amelioration and/or prevention of post-weaning diarrhea (PWD) of animals, such as pigs.
• PWD post-weaning diarrhea
• animals such as pigs.
• PWD is often caused by strains of Escherichia coll in the gastrointestinal tract of animals (e.g. Fairbrother et al. 2005. Anim Health Res Rev. 6(1):17-39), resulting in considerable economic loss and animal malady.
• growth of such strains can be reduced in the gastrointestinal tract of animals (e.g. pigs) by administering to the animals the composition described herein, and thereby PWD can be treated, ameliorated and/or prevented.
• the composition modulates growth of microorganisms in a synergistic model, i.e., it provides observed value (“O”) beyond the expected value (“E”) resulting from the individual ingredient of the composition, i.e, benzoic acid and/or salt thereof and the additional organic acid and/or salt thereof, in modulating growth of microorganisms.
• a synergistic model i.e., it provides observed value (“O”) beyond the expected value (“E”) resulting from the individual ingredient of the composition, i.e, benzoic acid and/or salt thereof and the additional organic acid and/or salt thereof, in modulating growth of microorganisms.
• the present invention provides a method for modulating growth of microorganisms, i.e., inhibiting the growth of pathogenic microorganisms and/or enhancing the growth of beneficial (i.e. probiotic) microorganisms, wherein the method comprises the steps of a) providing a composition as described herein; and b) contacting the composition of a) with the microorganisms.
• the present invention provides a method for modulating growth of microorganisms, especially for inhibiting the growth of pathogenic microorganisms, in a food and/or a feed, wherein the method comprises the steps of a) providing a composition as described herein; and b) contacting the composition of a) with the microorganisms in the food and/or the feed.
• the present invention provides a method for modulating growth of microorganisms in the gastrointestinal tracts, especially in the intestinal tracts (e.g. stomach, duodenum, jejunum, illeum, cecum, and/or large intestine) of animals and/or improving gut health of animals, wherein the method comprises the steps of a) providing a composition as described herein; and b) administering to the animals the composition of a).
• the intestinal tracts e.g. stomach, duodenum, jejunum, illeum, cecum, and/or large intestine
• the present invention provides a method for the treatment, amelioration and/or prevention of post-weaning diarrhea (PWD) of animals, such as pigs, wherein the method comprises the steps of a) providing a composition as described herein; and b) administering to the animals the composition of a).
• PWD post-weaning diarrhea
• the present invention provides a method for improving growth performance of animals, wherein the method comprises the steps of a) providing a composition as described herein; and b) administering to the animals the composition of a).
• the composition modulates growth of microorganisms, and/or treats, ameliorates and/or prevent PWD of animals, and/or improves growth performance of animals in a synergistic model, i.e., it provides observed value (“O”) beyond the expected value (“E”) resulting from the individual ingredient of the composition, i.e, benzoic acid and/or salt thereof and the additional organic acid and/or salt thereof, in modulating growth of microorganisms, and/or treatment, amelioration and/or prevention of PWD of animals, and/or improving growth performance of animals.
• a synergistic model i.e., it provides observed value (“O”) beyond the expected value (“E”) resulting from the individual ingredient of the composition, i.e, benzoic acid and/or salt thereof and the additional organic acid and/or salt thereof, in modulating growth of microorganisms, and/or treatment, amelioration and/or prevention of PWD of animals, and/or improving growth performance of animals.
• the present invention provides use of at least one additional organic acid and/or at least one salt thereof in the composition as defined above for boosting the effect of benzoic acid and/or at least one salt thereof in modulating growth of microorganisms, i.e., inhibiting the growth of pathogenic microorganisms and/or enhancing the growth of beneficial microorganisms,
• the present invention provides a method for boosting the effect of benzoic acid and/or at least one salt thereof in modulating growth of microorganisms, i.e., inhibiting the growth of pathogenic microorganisms and/or enhancing the growth of beneficial microorganisms, wherein the method comprises: a) providing a composition as described herein; and b) contacting the composition of a) with the microorganisms.
• the present invention provides a method for boosting the effect of benzoic acid and/or at least one salt thereof in modulating growth of microorganisms in the gastrointestinal tracts, especially in the intestinal tracts (e.g.
• PWD post-weaning diarrhea
• the term “boost” or “boosting” means that benzoic acid and/or at least one salt thereof and the at least one additional organic acid and/or at least one salt thereof in the composition according to the present invention modulates growth of microorganisms in a synergistic model, i.e., it provides observed value (“O”) beyond the expected value (“E”) resulting from the individual ingredient of the composition, i.e, benzoic acid and/orsaltthereof and the additional organic acid and/or salt thereof, in modulating growth of microorganisms.
• S represents the difference in the amount of inhibition or enhancement between the calculated additive inhibition (“E”) and the actually observed amount of inhibition or enhancement (“O”); “E” represents the expectation value as described above; and “O” denotes the observed amount of inhibition or enhancement.
• E calculated additive inhibition
• O actually observed amount of inhibition or enhancement
• S is a positive number, i.e. larger than 0, a combination of substance 1 and substance 2 was confirmed to inhibit or enhance the growth of the studied microorganisms synergistically.

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• 2023
  • 2023-11-15 EP EP23805975.2A patent/EP4626257A1/de active Pending
  • 2023-11-15 WO PCT/EP2023/081899 patent/WO2024115111A1/en not_active Ceased
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