EP4540359A1 - Modular skalierbarer photobioreaktor - Google Patents
Modular skalierbarer photobioreaktorInfo
- Publication number
- EP4540359A1 EP4540359A1 EP23733244.0A EP23733244A EP4540359A1 EP 4540359 A1 EP4540359 A1 EP 4540359A1 EP 23733244 A EP23733244 A EP 23733244A EP 4540359 A1 EP4540359 A1 EP 4540359A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- photobioreactor
- reactor
- cultivation
- reactor compartment
- end piece
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/44—Multiple separable units; Modules
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/10—Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
Definitions
- the present invention relates to a modular photobioreactor, in particular an artificially illuminated modular and scalable photobioreactor, which is suitable for the cultivation of phototrophic microorganisms in an industrial production environment with a high degree of automation.
- the photobioreactors according to the invention enable the highest biomass and product productivity over a long period of time with optimal biomass quality and low investment, maintenance and operating costs.
- a major disadvantage of industrial flat plate systems is still that the reactor chambers of flat plate reactors cannot physically be designed to be as wide and high as desired, otherwise the manufacturing costs will rise sharply.
- an arrangement of many individual flat plates in a system network is therefore required from an economic point of view.
- Several reactors can be connected one behind the other to form a module network in order, for example, to be able to scale from a preculture to the desired production volume as quickly as possible or to optimally coordinate multi-stage processes.
- the individual chambers of such reactors are usually connected to one another via external lines to form a total volume.
- the chambers are often not optimally mixed in order to compensate for the concentration gradients that develop during cultivation.
- a particular danger in scaling flat plate systems is that choosing a production volume that is too large can result in total loss in the event of contamination the entire batch can come. Accurate monitoring means a lot of effort for the system operator and a high investment requirement for online and offline measurement technology.
- the biomass concentration, or the geometry of the reactor volume shift accordingly.
- the light is completely absorbed in the first few millimeters; at low cell concentrations, transmission can also occur through the cultivation volume.
- This relationship characterizes the achievable photosynthesis rate and consequently the performance of the entire reactor. Since the reactor is not a static system, the cells move between the zones described. Depending on the mixing strategy used, the cells move statistically along certain trajectories through the reactor volume and are supplied with a light pulse at a certain frequency on the surface in order to then return to the shaded areas of the reactor. The faster this process occurs, the more cells are statistically supplied with light.
- a change of fluid mechanics statistically leads to characteristic light/dark cycles in the volume, which represent a measure of the performance of a reactor. This results in two borderline cases which, depending on the organism used, can lead to good growth in one or the other configuration of the reactor system.
- a reactor with a greater layer thickness and strong structuring of its geometry can be mixed either by pumping or gassing in order to guide the cells through the reactor on certain light/dark trajectories through the turbulence. In this way, a high biomass concentration can be achieved.
- the layer thickness of the reactor can be greatly reduced with an extreme surface area to volume (O/V) ratio.
- O/V extreme surface area to volume
- the present invention is based on the technical problem of overcoming the disadvantages of the photobioreactors described in the prior art and of meeting the high demands on photobioreactors for the efficient cultivation of phototrophic microorganisms on an industrial scale.
- the present invention solves the underlying technical problem through the subject matter of the independent claims.
- the invention relates in particular to a modular photobioreactor, comprising a first end piece, a second end piece and at least one reactor compartment arranged between the first end piece and the second end piece, which is characterized in that the photobioreactor comprises at least two cultivation chambers, wherein the at least two cultivation chambers positive joining of the two end pieces and the at least one reactor compartment are formed, the individual cultivation chambers of the photobioreactor being in fluid communication with one another and the two end pieces and the at least one reactor compartment each comprising at least one light source.
- the invention relates in particular to a modular photobioreactor, comprising a first end piece, a second end piece and at least one reactor compartment arranged between the first end piece and the second end piece, wherein the photobioreactor at least comprises two cultivation chambers, wherein the at least two cultivation chambers are formed by positively joining the two end pieces and the at least one reactor compartment, the individual cultivation chambers of the photobioreactor being in fluid communication with one another and the two end pieces and the at least one reactor compartment each comprising at least one light source, thereby characterized in that the at least one reactor compartment has two shell elements which are connected to one another in a back-to-back arrangement.
- the modular and expandable structure of the photobioreactor according to the invention allows the number of reactor compartments arranged between the end pieces and the associated cultivation chambers of the photobioreactor to be increased or reduced according to requirements.
- the presence of at least one light source, in particular at least one artificial light source, on the two end pieces and the at least one reactor compartment allows a daylight-independent, uniform supply of light energy to phototrophic microorganisms located in the cultivation chambers, a compact and space-saving structure of the reactor and the modular scalability of the photobioreactor without Loss of performance of the reactor due to shading of the cultured cells and the associated reduction in their photosynthesis performance as the number of reactor compartments arranged next to one another increases.
- a first cultivation chamber is formed by positively joining the first end piece to the at least one reactor compartment and a second cultivation chamber is formed by positively joining the second end piece to the at least one reactor compartment.
- the modular photobioreactor comprises a first end piece, a second end piece and at least two reactor compartments arranged between the first end piece and the second end piece, the photobioreactor comprising at least three cultivation chambers, the at least three cultivation chambers being positively joined together the two end pieces and the at least two reactor compartments are formed, the individual cultivation chambers of the photobioreactor being in fluid communication with one another and the two end pieces and the at least two reactor compartments each comprising at least one light source.
- a first cultivation chamber is formed by positively joining the first end piece to a first reactor compartment
- a second cultivation chamber is formed by positively joining the first reactor compartment to the second reactor compartment
- a third cultivation chamber is formed by positively joining the second reactor compartment to the second end piece .
- the photobioreactor according to the invention preferably has at least 2, preferably at least 3, preferably at least 4, preferably at least 5, preferably at least 6, preferably at least 7, preferably at least 8, preferably at least 9, preferably at least 10, preferably at least 15, preferably at least 20, preferably at least 25, reactor compartments arranged between the first end piece and the second end piece.
- the photobioreactor has at most 50, preferably at most 45, preferably at most 40, preferably at most 35, preferably at most 30, preferably at most 25, preferably at most 20, preferably at most 15, preferably at most 10, preferably at most 9, preferably at most 8, preferably at most 7, preferably at most 6, preferably at most 5, preferably at most 4, preferably at most 3, preferably at most 2, reactor compartments arranged between the first end piece and the second end piece.
- the photobioreactor has at least 3, preferably at least 4, preferably at least 5, preferably at least 6, preferably at least 7, preferably at least 8, preferably at least 9, preferably at least 10, preferably at least 15, preferably at least 20 at least 25 cultivation chambers.
- the photobioreactor has at most 50, preferably at most 45, preferably at most 40, preferably at most 35, preferably at most 30, preferably at most 25, preferably at most 20, preferably at most 15, preferably at most 10, preferably at most 9, preferably at most 8, preferably at most 7, preferably at most 6, preferably at most 5, preferably at most 4, preferably at most 3, cultivation chambers.
- the photobioreactor according to the invention preferably has a first end piece, a second end piece, and n reactor compartments arranged between the first end piece and the second end piece and n+1 cultivation combs, where n > 1, preferably n > 2, preferably n > 3, preferably n > 4, preferably n > 5, preferably n > 6, preferably n > 7, preferably n > 8, preferably n > 9, preferably n > 10, preferably n > 15, preferably n > 20, preferably n > 25.
- the photobioreactor according to the invention has a first end piece, a second end piece, n reactor compartments arranged between the first end piece and the second end piece, and n+1 cultivation chambers, where n ⁇ 50, preferably n ⁇ 45, preferably n ⁇ 40, preferably n ⁇ 35, preferably n ⁇ 30, preferably n ⁇ 25, preferably n ⁇ 20, preferably n ⁇ 15, preferably n ⁇ 14, preferably n ⁇ 13, preferably n ⁇ 12, preferably n ⁇ 11, preferred n ⁇ 10, preferably n ⁇ 9, preferably n ⁇ 8, preferably n ⁇ 7, preferably n ⁇ 6, preferably n ⁇ 5.
- the modular photobioreactor comprises a first end piece, a second end piece and n reactor compartments arranged between the first end piece and the second end piece, the photobioreactor comprising n+1 cultivation chambers, the n+1 cultivation chambers having a positive fit Combining the two end pieces and the n reactor compartments are formed, the individual cultivation chambers of the photobioreactor being in fluid communication with one another, the two end pieces and the n reactor compartments each comprising at least one light source, and where n > 1, preferably n > 2, preferably > 3, preferably > 4, preferably n > 5, preferably > 6, preferably > 7, preferably n > 8, preferably > 9, preferably > 10, preferably n > 15, preferably n > 20, preferably n > 25.
- the present invention relates in particular to a modular photobioreactor, comprising a first end piece, a second end piece and at least n reactor compartments arranged between the first end piece and the second end piece, wherein the photobioreactor comprises at least n + 1 cultivation chambers, wherein the at least n + 1 cultivation chambers are formed by positively joining the two end pieces and the n reactor compartments, the individual cultivation chambers of the photobioreactor being in fluid communication with one another, the two end pieces and the n reactor compartments each comprising at least one light source, and where the following applies: n > 1, preferably > 2, preferably >3, preferably >4, preferably n >5, preferably >6, preferably >7, preferably n >8, preferably >9, preferably >10, preferably n >15, preferably n >20, preferably n According to a preferred embodiment, each of the cultivation chambers of the photobioreactor has at least one upflow area, preferably at least two upflow areas, preferably at least three upflow areas,
- each of the cultivation chambers has at least one Ab stream region, preferably at least two Ab stream regions, preferably at least three Ab stream regions, preferably at least four Ab stream regions.
- Each of the cultivation chambers preferably has at least two upflow areas and at least two downflow areas.
- the at least one flow area of the cultivation chambers is gassed.
- the gassing of the at least one upstream region of the cultivation chambers takes place via a slotted or perforated membrane, in particular via a slotted or perforated silicone membrane.
- the gassing of the at least one upstream region of the cultivation chambers takes place via a porous plastic or ceramic element.
- the gassing of the upstream areas of the cultivation chambers takes place via a common gassing channel which runs in the footwell of the individual reactor compartments.
- the at least one upstream region of the cultivation chambers is gassed with a gas mixture, in particular a CO2/air mixture.
- the CCh content in the gas mixture is preferably at least 0.5%, preferably at least 1%, preferably at least 2%, preferably at least 3%, preferably at least 4%, preferably at least 5%, preferably at least 6%, preferably at least 7%, preferably at least 8%, preferably at least 9%.
- the CCh content in the gas mixture, in particular in the CO2/air mixture is at most 10%, preferably at most 9%, preferably at most 8%, preferably at most 7%, preferably at most 6%, preferably at most 5%, preferably at most 4%, preferably at most 5%, preferably at most 4%, preferably at most 3%, preferably at most 2%, preferably at most 1%.
- the CCh content in the gas mixture, in particular in the CCb/air mixture is particularly preferably 0.5 to 10%, preferably 1 to 9%, preferably 2 to 8%, preferably 3 to 7%, preferably 4 to 6%.
- the gassing rate is at least 0.01 vvm (vol. gas per volume of culture medium per min), preferably at least 0.025 vvm, preferably at least 0.05 vvm, preferably at least 0.1 vvm, preferably at least 0.2 vvm, preferably at least 0.3 wm, preferably at least 0.4 vvm, preferably at least 0.5 vvm, preferably at least 0.6 wm, preferably at least 0.7 vvm, preferably at least 0.8 vvm, preferably at least 0.9 wm, preferred at least 1 vvm.
- the gassing rate is preferably at most 1 vvm (vol. gas per volume of culture medium per min), preferably at most 0.9 vvm, preferably at most 0.8 vvm, preferably at most 0.7 vvm, preferably at most 0.6 vvm, preferably at most 0, 5 vvm, preferably at most 0.4 vvm, preferably at most 0.3 vvm, preferably at most 0.2 vvm, preferably at most 0.1 vvm, preferably at most 0.05 vvm.
- the gassing rate is 0.01 to 1 vvm (vol. gas per volume of culture medium per min), preferably 0.025 to 0.8 vvm, preferably 0.05 to 0.6 vvm, preferably 0 .1 to 0.5 vvm, preferably 0.15 to 0.3 vvm.
- the gas mixture in particular the CO2/air mixture
- the recirculated gas mixture in particular the CO2/air mixture
- the recirculated gas mixture is enriched again with CO2 before the renewed gassing.
- the at least one downstream region of a cultivation chamber opens into at least one downstream region of at least one adjacent cultivation chamber.
- the downstream region of a cultivation chamber opens into at least one upstream region of at least one adjacent cultivation chamber via at least one channel.
- the at least one channel connecting the downstream region of a cultivation chamber with the at least one downstream region of at least one adjacent cultivation chamber runs below the at least one light source of the reactor compartments.
- the cultivation volume is exchanged between the individual cultivation chambers of the photobioreactor.
- the modular photobioreactor has no pump, in particular no pump for mixing the cultivation volume.
- the cultivation volume is preferably mixed without active pumping.
- the mixing of the cultivation volume is particularly preferably carried out pneumatically, in particular by moving the cultivation volume through the upflow areas and the downflow areas of the photobioreactor, in particular according to the principle of mammoth pump delivery (airlift principle).
- the photobioreactor according to the invention advantageously also allows the cultivation of shear-sensitive organisms, in particular shear-sensitive phototrophic microorganisms.
- the pneumatic mixing reduces the operating, maintenance and installation costs of the photobioreactor and thus increases the economic efficiency of the photobioreactor.
- the fluid connection between the individual cultivation chambers advantageously enables the cultivation volume to be mixed across all cultivation chambers of the photobioreactor.
- the mixing of the cultivation volume in the cultivation chambers of the photobioreactor, which are in fluid communication with one another is driven pneumatically, in particular according to the principle of mammoth pump delivery (airlift principle), that is, driven by the introduction of gas into the lower region of the at least one upflow region of a cultivation chamber , which then rises in the form of gas bubbles and leads to a hydrostatic pressure difference, which in turn results in an upward flow of the cultivation volume.
- the cultivation volume flows from at least one downstream region of the cultivation chamber into the upstream region of the at least one adjacent cultivation chamber and thus the cultivation volume is mixed between the individual cultivation chambers of the photobioreactor. In this way, an even supply of light and nutrients to cultivated phototrophic microorganisms is advantageously ensured and the formation of local concentration gradients in certain areas of the photobioreactor is prevented.
- the at least one light source comprises warm white LEDs and/or cold white LEDs, in particular equal proportions of warm white LEDs and cold white LEDs.
- the warm white LEDs preferably have a color temperature of 1500 to 4500 K, preferably 1750 to 3500 K, preferably 2000 to 3000 K.
- the cold white LEDs preferably have a color temperature of 4750 to 8000 K, preferably 5000 to 7000 K, preferably 5250 to 6000 K.
- the at least one light source comprises warm white LEDs and/or cool white LEDs and additionally blue LEDs and/or red LEDs.
- the at least one light source comprises warm white LEDs, cold white LEDs and red LEDs.
- the at least one light source comprises warm white LEDs, cold white LEDs and blue LEDs.
- the at least one light source includes warm white LEDs, cool white LEDs, red LEDs and blue LEDs.
- the warm white LEDs, cold white LEDs, red LEDs and/or blue LEDs are particularly preferably installed on a common carrier, in particular on a common circuit board.
- the at least one light source comprises warm white LEDs, cold white LEDs, blue LEDs and red LEDs arranged on a common carrier, in particular on a common circuit board, which can preferably be switched separately and together.
- the at least one light source comprises at least 50%, preferably at least 55%, preferably at least 60%, preferably at least 65%, preferably at least 70%, preferably at least 75%, white LEDs, in particular warm white and cool white LEDs.
- the ratio of warm white LEDs to cold white LEDs of the at least one light source is 50:50, preferably 55:45, preferably 60:40, preferably 65:35, preferably 70:30, preferably 75:25.
- the at least one light source comprises white LEDs, of which 50 to 80%, preferably 55 to 75%, preferably 60 to 70%, are warm white LEDs and 20 to 50%, preferably 25 to 45%, preferably 30 to 40%, are cool white LEDs are.
- the at least one light source comprises 0 to 25% blue LEDs and 75 to 100% red LEDs.
- the ratio of red LEDs to blue LEDs of the at least one light source is at least 1:3, preferably at least 1:4, preferably at least 1:5, preferably at least 1:6.
- the luminous efficacy of the at least one light source is at least 150 Im/W (lumens per watt), preferably at least 175 Im/W, preferably at least 200 Im/W, preferably at least 210 Im/W, preferably at least 220 Im/W, preferably at least 230 Im/W, preferably at least 240 Im/W, preferably at least 250 Im/W.
- the irradiance of the at least one light source is at least 50 W/m 2 (watts per square meter), preferably at least 75 W/m 2 , preferably at least 100 W/m 2 , preferably at least 125 W/m 2 , preferably at least 150 W/m 2 , preferably at least 175 W/m 2 , preferably at least 200 W/m 2 , preferably at least 225 W/m 2 , preferably at least 250 W/m 2 , preferably at least 275 W/m 2 , preferably at least 300 W/m 2 m 2 , preferably at least 325 W/m 2 , preferably at least 350 W/m 2 .
- the irradiance of the at least one light source is at most 400 W/m 2 (watts per square meter), preferably at most 375 W/m 2 , preferably at most 350 W/m 2 , preferably at most 325 W/m 2 , preferably at most 300 W/m 2 , preferably at most 275 W/m 2 , preferably at most 250 W/m 2 , preferably at most 225 W/m 2 , preferably at most 200 W/m 2 .
- the irradiance of the at least one light source is particularly preferably 50 to 400 W/m 2 , preferably 100 to 350 W/m 2 , preferably 150 to 300 W/m 2 , preferably 175 to 275 W/m 2 , preferably 200 to 250 W/ m2 .
- the at least one light source is part of an illumination plane arranged between two shell elements of a reactor compartment.
- the illumination plane comprising at least one light source and arranged between two shell elements of a reactor compartment radiates in two opposite directions.
- the illumination plane arranged between two shell elements of a reactor compartment comprises at least two light sources which are arranged back to back to one another and radiate in opposite directions.
- a cooling device is arranged between the at least two light sources of an illumination level.
- the illumination plane has an area of at least 0.5 m 2 , preferably at least 0.6 m 2 , preferably at least 0.7 m 2 , preferably at least 0.8 m 2 , preferably at least 0.9 m 2 , preferably at least 1 m 2 .
- the at least one light source in particular the at least one light source of the illumination plane arranged between two shell elements of a reactor compartment, has a distance of at most 5 cm, preferably at most 4.5 cm, preferably at most 4 cm, preferably at most 3, 5 cm, preferably at most 3 cm, preferably at most 2.5 cm, preferably at most 2 cm, preferably at most 1.5 cm, preferably at most 1 cm, preferably at most 0.5 cm, from the surface of the cultivation chambers of the photobioreactor.
- the at least one light source in particular the at least one light source of the illumination plane arranged between two shell elements of a reactor compartment, has a distance of at least 0.5 cm, preferably at least 0.75 cm, preferably at least 1 cm, preferably at least 1.25 cm at least 1.5 cm, preferably at least 1.75 cm, preferably at least 2 cm, preferably at least 2.25 cm, preferably at least 2.5 cm, preferably at least 2.75 cm, preferably at least 3 cm, from the surface of the cultivation chambers of photobioreactor.
- the at least one light source in particular the at least one light source of the illumination plane arranged between two shell elements of a reactor compartment, has a distance of 0.5 to 5 cm, 0.75 to 4 cm, preferably 1 to 3.5 cm, preferably 1, 25 to 3 cm, preferably 1.5 to 2.5 cm, from the surface of the cultivation chambers of the photobioreactor.
- the at least one reactor compartment has two shell elements.
- the at least one reactor compartment consists of two shell elements.
- the two shell elements of the at least one reactor compartment are preferably connected to one another in a back-to-back arrangement.
- an illumination plane comprising the at least one light source of the reactor compartment is arranged between the two shell elements.
- a reactor compartment of the photobioreactor according to the invention has two shell elements which are connected to one another in a back-to-back arrangement, with an illumination plane comprising the at least one light source of the reactor compartment being located between the two shell elements.
- the two shell elements of the at least one reactor compartment are half-shells.
- the two shell elements, in particular the two half-shells, of a reactor compartment are designed to be mirror-symmetrical to one another. According to this preferred embodiment of the present invention, it can be provided that the mirror plane of the reactor compartment lies in the illumination plane arranged between the two shell elements.
- the two shell elements, in particular the two half-shells, of a reactor compartment are not designed to be mirror-symmetrical to one another.
- the first end piece comprises a shell element, in particular a half shell.
- the first end piece comprises a shell element, in particular a half-shell, and at least one light source.
- the second end piece comprises a shell element, in particular a half shell.
- the second end piece comprises a shell element, in particular a half-shell, and at least one light source.
- the first and second end pieces each comprise a shell element, in particular a half-shell.
- the first and second end pieces each comprise a shell element, in particular a half-shell, and at least one light source.
- each of the two shell elements, preferably each of the two half-shells, of a reactor compartment has a shell element, preferably a half-shell, a further reactor compartment or a shell element, preferably a half-shell, an end piece Cultivation chamber forms.
- the cultivation chambers formed by the shell elements are sealed by means of a circumferential seal.
- the two end pieces and the at least one reactor compartment arranged between the two end pieces are arranged horizontally next to one another.
- the at least two cultivation chambers, in particular the n+1 cultivation chambers are arranged horizontally next to one another.
- the photobioreactor according to the invention comprises, in addition to the two end pieces and the at least one reactor compartment, in particular in addition to the two end pieces and the at least n reactor compartments, two cover plates arranged at the ends.
- the two end pieces and the at least one reactor compartment, in particular the two end pieces and the at least n reactor compartments are arranged horizontally next to one another and are pressed together via the end cover plates.
- the end pieces and the reactor compartments arranged next to one another between the first and second end pieces are pressed together hydraulically or by screwing, preferably via end-mounted cover plates.
- the formation of a closed reactor space that is delimited from the environment is achieved.
- the force exerted on the end pieces preferably the force exerted on the end pieces by means of the cover plates, corresponds to at least the spring restoring force of all reactor compartments and the two end pieces.
- the at least one reactor compartment of the photobioreactor according to the invention preferably has a height of at least 0.25 m, preferably at least 0.5 m, preferably at least 0.75 m, preferably at least 1 m, preferably at least 1.25 m, preferably at least 1.5 m , preferably at least 1.75 m, preferably at least 2 m.
- the at least one reactor compartment of the photobioreactor according to the invention has a height of at most 5 m, preferably at most 4.5 m, preferably at most 4 m, preferably at most 3.5 m, preferably at most 3 m, preferably at most 2.5 m, preferably at most 2 m, preferably at most 1.5 m.
- the at least one reactor compartment has a height of 0.25 to 5 m, preferably 0.5 to 4.5 m, preferably 0.75 to 4 m, preferably 1 to 3.5 m, preferably 1.25 to 3 m, preferably 1.5 to 2.5 m.
- the first and second end pieces of the photobioreactor according to the invention preferably have a height of at least 0.25 m, preferably at least 0.5 m, preferably at least 0.75 m, preferably at least 1 m, preferably at least 1.25 m, preferably at least 1.5 m, preferably at least 1.75 m, preferably at least 2 m.
- the first and second end pieces of the photobioreactor according to the invention have a height of at most 5 m, preferably at most 4.5 m, preferably at most 4 m, preferably at most 3.5 m, preferably at most 3 m, preferably at most 2.5 m , preferably at most 2 m, preferably at most 1.5 m.
- the first and second end pieces of the photobioreactor according to the invention have a height of 0.25 to 5 m, preferably 0.5 to 4.5 m, preferably 0.75 to 4 m, preferably 1 to 3, 5 m, preferably 1.25 to 3 m, preferably 1.5 to 2.5 m.
- the at least one reactor compartment of the photobioreactor according to the invention preferably has a width of at least 0.25 m, preferably at least 0.5 m, preferably at least 0.75 m, preferably at least 1 m, preferably at least 1.25 m, preferably at least 1.5 m , preferably at least 1.75 m, preferably at least 2 m.
- the at least one reactor compartment of the photobioreactor according to the invention has a width of at most 5 m, preferably at most 4.5 m, preferably at most 4 m, preferably at most 3.5 m, preferably at most 3 m, preferably at most 2.5 m, preferably at most 2 m, preferably at most 1.5 m.
- the at least one reactor compartment has a width of 0.25 to 5 m, preferably 0.5 to 4.5 m, preferably 0.75 to 4 m, preferably 1 to 3.5 m, preferably 1.25 to 3 m, preferably 1.5 to 2.5 m.
- the first and second end pieces of the photobioreactor according to the invention preferably have a width of at least 0.25 m, preferably at least 0.5 m, preferably at least 0.75 m, preferably at least 1 m, preferably at least 1.25 m, preferably at least 1.5 m, preferably at least 1.75 m, preferably at least 2 m.
- the first and second end pieces of the photobioreactor according to the invention have a width of at most 5 m, preferably at most 4.5 m, preferably at most 4 m, preferably at most 3.5 m, preferably at most 3 m, preferably at most 2.5 m , preferably at most 2 m, preferably at most 1.5 m.
- the first and second end pieces of the photobioreactor according to the invention have a width of 0.25 to 5 m, preferably 0.5 to 4.5 m, preferably 0.75 to 4 m, preferably 1 to 3, 5 m, preferably 1.25 to 3 m, preferably 1.5 to 2.5 m.
- the cultivation chambers in particular the cultivation chambers formed by the two end pieces and the reactor compartments arranged between the first and second end pieces, have a maximum chamber depth, in particular maximum layer thickness, of at least 0.5 cm, preferably at least 1 cm, preferably at least 1.5 cm, preferably at least 2 cm, preferably at least 2.5 cm, preferably at least 3 cm, preferably at least 3.5 cm, preferably at least 4 cm, preferably at least 4.5 cm, preferably at least 5 cm.
- the cultivation chambers in particular the cultivation chambers formed by the two end pieces and the reactor compartments arranged between the first and second end pieces, have a maximum chamber depth, in particular maximum layer thickness, of at most 10 cm, preferably at most 9 cm, preferably at most 8 cm, preferably at most 7 cm, preferably at most 6 cm, preferably at most 5 cm, preferably at most 4.5 cm, preferably at most 4 cm, preferably at most 3.5 cm, preferably at most 3 cm, preferably at most 2.5 cm, preferred at most 2 cm, preferably at most 1.5 cm.
- the cultivation chambers in particular the cultivation chambers formed by the two end pieces and the reactor compartments arranged between the first and second end pieces, have a non-uniform chamber depth, in particular non-uniform layer thickness.
- the cultivation chambers in particular the cultivation chambers formed by the two end pieces and the reactor compartments arranged between the first and second end pieces, have a non-uniform chamber depth, in particular non-uniform layer thickness, in the range of 0.5 to 10 cm, preferably 0.5 to 7.5 cm, preferably 0.5 to 5 cm, preferably 1 to 4.5 cm, preferably 1 to 4 cm, preferably 1.5 to 3.5 cm, preferably 1.5 to 3 cm.
- the cultivation chambers each have a volume of at least 5 liters, preferably at least 10 liters, preferably at least 20 liters, preferably at least 30 liters, preferably at least 40 liters, preferably at least 50 liters, preferably at least 75 liters, preferably at least 100 liters, preferably at least 150 liters, preferably at least 200 liters, preferably at least 250 Liters, preferably at least 500 liters, preferably at least 750 liters, preferably at least 1000 liters, preferably at least 1500 liters, preferably at least 2000 liters, preferably at least 2500 liters, preferably at least 3000 liters, preferably at least 3500 liters, preferably at least 4000 liters, preferably at least 5000 liters, on.
- the cultivation chambers each have a volume of at most 20,000 liters, preferably at most 15,000 liters, preferably at most 10,000 liters, preferably at most 7,500 Liters, preferably at most 5000 liters, preferably at most 4000 liters, preferably at most 3000 liters, preferably at most 2500 liters, preferably at most 2000 liters, preferably at most 1500 liters, preferably at most 1000 liters, preferably at most 750 liters, preferably at most 500 liters, preferably at most 400 Liters, preferably at most 300 liters, preferably at most 200 liters, preferably at most 150 liters, preferably at most 100 liters, preferably at most 75 liters, preferably at most 50 liters.
- the cultivation chambers in particular the cultivation chambers formed by the two end pieces and the at least one reactor compartment arranged between the first and second end pieces, each have a volume of 5 to 20,000 liters, preferably 5 to 10,000 liters, preferably 10 to 7,500 liters, preferably 10 to 5000 liters, preferably 20 to 2500 liters, preferably 20 to 1000 liters, preferably 30 to 500 liters, preferably 30 to 250 liters.
- the photobioreactor according to the invention has a total volume of at least 100 liters, preferably at least 150 liters, preferably at least 200 liters, preferably at least 250 liters, preferably at least 300 liters, preferably at least 350 liters, preferably at least 400 liters, preferably at least 450 liters , preferably at least 500 liters, preferably at least 750 liters, preferably at least 1000 liters, preferably at least 2500 liters, preferably at least 5000 liters, preferably at least 7500 liters, preferably at least 10,000 liters.
- the cultivation chambers formed by shell elements, in particular by the half-shells, of the reactor compartments are segmented.
- at least one Upstream area of the cultivation chambers formed by the shell elements, in particular by the half-shells, of the reactor compartments is segmented.
- the reactor compartments, in particular the shell elements, preferably the half-shells, of the reactor compartments have a horizontal segmentation in the at least one upflow region, in particular a horizontal segmentation in the form of reactor half-shells arranged horizontally one above the other.
- the horizontal segmentation of the shell elements, preferably the half-shells, of the reactor compartments in particular the horizontal segmentation in the form of reactor half-shells arranged horizontally one above the other, in the assembled state of the photobioreactor, static mixers are formed in the at least one upstream region of the cultivation chambers.
- the cultivation chambers of the photobioreactor in particular the at least one upstream region of the cultivation chambers, preferably have at least 2, preferably at least 3, preferably at least 4, preferably at least 5, preferably at least 6, preferably at least
- the cultivation chambers of the photobioreactor in particular the at least one upflow region of the cultivation chambers, have at most 100, preferably at most 90, preferably at most 80, preferably at most 70, preferably at most 60, preferably at most 50, preferably at most 40, preferably at most 35, preferably at most 30, preferably at most 25, preferably at most 20, preferably at most 15, preferably at most 10, preferably at most 9, preferably at most
- the present invention also relates to a method for cultivating phototrophic microorganisms, comprising the steps: a) provision of a culture medium comprising at least one phototrophic microorganism, b) provision of a modular photobioreactor according to the invention, c) cultivation of the at least one phototrophic microorganism in the modular photobioreactor according to the invention.
- a further aspect of the present invention relates to the use of a modular photobioreactor according to the invention for cultivating phototrophic microorganisms.
- the term “modular photobioreactor” is understood to mean a bioreactor suitable for the cultivation of phototrophic microorganisms, which is characterized in that the bioreactor is made up of several components, in particular two end pieces and at least one reactor compartment arranged between the two end pieces, and is constructed accordingly can be scaled to the requirements placed on it by removing or adding individual components, in particular individual reactor compartments.
- the “modular structure” of the photobioreactor according to the invention also allows the photobioreactor to be easily assembled and disassembled for maintenance and maintenance purposes.
- the “modular photobioreactor” is characterized in that it comprises a first and a second end piece, between which individual reactor compartments can be arranged, with cultivation chambers being formed by positive joining of the two end pieces with the individual reactor compartments arranged between them, which are connected to one another are in fluid communication, so that the total volume of the photobioreactor can be scaled by the number of reactor compartments arranged between the two end pieces.
- the term “cultivation chamber” is used for a delimited space defined by walls within the modular photobioreactor according to the invention, which has a volume that is suitable for holding culture medium and for cultivating phototrophic microorganisms located in the culture medium.
- the individual “cultivation chambers” of the photobioreactor according to the invention are preferably characterized in that they are formed by positively joining the two end pieces and the at least one reactor compartment.
- the “cultivation chambers” particularly preferably have at least one upflow area and at least one downflow area.
- a “reactor compartment” refers to a component of the modular photobioreactor according to the invention, which, together with a further “reactor compartment” and/or an end piece of the photobioreactor, forms at least one cultivation chamber by positive assembly.
- a reactor compartment has two shell elements, in particular two half-shells, which are arranged back to back to one another and are connected to one another.
- the term “riser” refers to a definable area of a cultivation chamber, within which introduced gas and culture medium located in the cultivation chamber are transported vertically upwards.
- the term “outflow area” (English: “Downcomer”/“Downer”) is understood to mean a definable area of a cultivation chamber, within which culture medium located in the cultivation chamber is transported vertically downwards.
- the term “warm white LED” is understood to mean a light-emitting diode that emits visible light with a color temperature in the range from 1500 to 4500 K, preferably 1750 to 3500 K, preferably 2000 to 3000 K, if electric current flows in the forward direction.
- the term “cold white LED” is used for a light-emitting diode that emits visible light with a color temperature in the range from 4750 to 8000 K, preferably 5000 to 7000 K, preferably 5250 to 6000 K, when electric current flows in the forward direction.
- blue LED is understood to mean a light-emitting diode that emits visible light with a wavelength in the range from 400 to 500 nm, preferably 410 to 490 nm, preferably 420 to 480 nm, preferably 430 to 470 nm, emitted when electric current flows in the forward direction.
- a “red LED” is understood to mean a light-emitting diode that emits visible light with a wavelength in the range from 600 to 700 nm, preferably 610 to 690 nm, preferably 620 to 680 nm, preferably 630 to 670 nm, emitted when electric current flows in the forward direction.
- the terms “comprising” and “having” mean that, in addition to the elements explicitly covered by these terms, there may be additional elements not explicitly mentioned. In the context of the present invention, these terms also mean that only the explicitly mentioned elements are recorded and no further elements are present. In this particular embodiment, the meaning of the terms “comprising” and “comprising” is synonymous with the term “consisting of”.
- compositions that, in addition to the explicitly named elements, also contain other elements not mentioned, but which are of a functionally and qualitatively subordinate nature.
- the terms “comprising” and “comprising” are synonymous with the term “consisting essentially of.”
- the term “and/or” is understood to mean that all members of a group which are connected by the term “and/or” are disclosed both alternatively to one another and cumulatively with one another in any combination.
- A, B and/or C this means that the following disclosure content is to be understood: a) A or B or C or b) (A and B) or c) (A and C) or d) ( B and C) or e) (A and B and C).
- first and second decimal places or the second decimal place are not specified in connection with the present invention, they must be set as 0. Further preferred embodiments result from the subclaims.
- FIG. 1A shows the top view of a modular photobioreactor (1) according to the invention comprising two end pieces (2), (5) and two reactor compartments (3), (4) in the unassembled state.
- Each of the reactor compartments (3), (4) has two shell elements (3a, 3b), (4a, 4b) which are connected to one another in a back-to-back arrangement and together with the shell elements (2a), (5a ) of the end pieces (2), (5) can be connected in a form-fitting manner.
- FIG. 1B shows the top view of a modular photobioreactor according to the invention in the assembled state.
- Each of the reactor compartments (3), (4) has two shell elements (3a, 3b, 4a, 4b) which are arranged back to back to one another.
- the cultivation chamber (10) By positively connecting the shell element (2a) of the first end piece (2) to the one shell element (3a) of the reactor compartment (3), the cultivation chamber (10), the two upflow areas (7a, 7b) and three downflow areas (6a, 6b, 6c).
- the cultivation chamber (20) which has three upflow areas (7c, 7d, 7e), is formed between the second shell element (3b) of the reactor compartment (3) and a shell element (4a) of the reactor compartment (4).
- a further cultivation chamber (30) with two upflow areas (7f, 7g) and three downflow areas (6f, 6g, 6h) is created by positively connecting the second shell element (4b) of the reactor compartment (4) to the shell element (5a) of the second end piece ( 5) trained.
- the arrows show the flow direction of the culture volume from the down stream areas (6a, 6b, 6c, 6d, 6e, 6f, 6g, 6h) of the cultivation chambers (10, 20, 30) of the photobioreactor (1) to the up stream areas (7a, 7b , 7c, 7d, 7e, 7f, 7g) of the adjacent cultivation chamber(s).
- the reactor compartments (3, 4) each have an illumination plane (8) comprising at least one light source arranged between the shell elements (3a, 3b, 4a, 4b).
- FIG 3 sections of a reactor compartment along the section planes AA and BB of Figure 2. It can be seen from the section along the section plane AA (left) that the upflow area (7) of the reactor compartment shown has static mixers (12) arranged one above the other in the form of horizontal extending reactor half shells.
- An illumination plane (8) comprising at least one light source is arranged between the two shell elements of the reactor compartment.
- each of the shell elements of a reactor compartment can comprise a circumferential seal (11).
- the section along the section plane BB shows the Ab flow area (6) of a reactor compartment.
- the culture volume is transported vertically through the downstream area (6) of the cultivation chamber, is passed under the illumination level (8), which is arranged between the two shell elements of the reactor compartment shown, and finally flows into the upstream area of the adjacent cultivation chamber.
- Figure 4 shows a section through the upflow areas (7) of three cultivation chambers (10, 20, 30) arranged next to one another, which are formed by positively joining together a first end piece (2), two reactor compartments (3, 4) and a second end piece (5). become.
- the two end pieces (2, 5) and the two reactor compartments (3, 4) each have one between the shell elements (3a, 3b, 4a, 4b) of the reactor compartments (3, 4) or on the shell elements (2a, 5a) of the two End pieces (2, 5) arranged at least one light source comprising illumination levels (8).
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Abstract
Description
Claims
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102022206019.9A DE102022206019A1 (de) | 2022-06-14 | 2022-06-14 | Modular skalierbarer Photobioreaktor |
| PCT/EP2023/065707 WO2023242148A1 (de) | 2022-06-14 | 2023-06-13 | Modular skalierbarer photobioreaktor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EP4540359A1 true EP4540359A1 (de) | 2025-04-23 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP23733244.0A Pending EP4540359A1 (de) | 2022-06-14 | 2023-06-13 | Modular skalierbarer photobioreaktor |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP4540359A1 (de) |
| JP (1) | JP2025519649A (de) |
| KR (1) | KR20250022699A (de) |
| CN (1) | CN119343440A (de) |
| AU (1) | AU2023293665A1 (de) |
| CA (1) | CA3257815A1 (de) |
| DE (1) | DE102022206019A1 (de) |
| WO (1) | WO2023242148A1 (de) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4653520A1 (de) * | 2024-05-21 | 2025-11-26 | Subitec GmbH | Bioreaktor und führungselement dafür |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10049437A1 (de) | 2000-10-06 | 2002-04-25 | Fraunhofer Ges Forschung | Bioreaktor für die Kultivierung von Mikroorganismen sowie Verfahren zur Herstellung desselben |
| BE1017763A5 (nl) | 2007-09-24 | 2009-06-02 | Proviron Holding | Bioreactor. |
| US7895790B2 (en) * | 2009-04-23 | 2011-03-01 | Chien-Feng Lin | Algae cultivation apparatus |
| DE102010021154A1 (de) * | 2010-05-21 | 2011-11-24 | Karlsruher Institut für Technologie | Photobioreaktor |
| DE102013109747A1 (de) * | 2013-09-06 | 2015-03-12 | Weber Gmbh | Vorrichtung sowie Verfahren zur Gewinnung von Phytoplankton (Mikroalgen) |
| AT515854B1 (de) * | 2014-05-30 | 2018-07-15 | Ecoduna Ag | Verfahren für einen photochemischen, wie photokatalytischen und/oder photosynthetischen Prozess |
| PT3653695T (pt) | 2015-05-13 | 2021-10-04 | Aaa Accelerator Group Europe Ag | Sistema modular de fotobiorreatores para cultivo de algas |
| GB201708940D0 (en) * | 2017-06-05 | 2017-07-19 | Arborea Ltd | Photo-bioreactor device and methods |
| CN106957790B (zh) * | 2017-04-13 | 2019-11-12 | 厦门中研融源科技有限公司 | 一种微藻藻种光生物反应半封闭式培养管道及其使用方法 |
| KR102200310B1 (ko) * | 2018-08-21 | 2021-01-08 | 농업회사법인 주식회사 스피루리나팜스 | 수직 이중관을 이용한 미세조류 광 배양장치 및 이를 갖는 농수산업용 융복합 건축 구조물 |
| DE102020122939A1 (de) * | 2020-09-02 | 2022-03-03 | Weber Gmbh | Modulare Vorrichtung zur Mikroalgengewinnung |
| DE102020127005A1 (de) * | 2020-10-14 | 2022-04-14 | Anita Meier | Photobioreaktor, insbesondere zur Produktion von Mikroorganismen wie beispielsweise Mikroalgen |
-
2022
- 2022-06-14 DE DE102022206019.9A patent/DE102022206019A1/de active Pending
-
2023
- 2023-06-13 KR KR1020247041955A patent/KR20250022699A/ko active Pending
- 2023-06-13 AU AU2023293665A patent/AU2023293665A1/en active Pending
- 2023-06-13 EP EP23733244.0A patent/EP4540359A1/de active Pending
- 2023-06-13 WO PCT/EP2023/065707 patent/WO2023242148A1/de not_active Ceased
- 2023-06-13 JP JP2024573277A patent/JP2025519649A/ja active Pending
- 2023-06-13 CA CA3257815A patent/CA3257815A1/en active Pending
- 2023-06-13 CN CN202380046139.7A patent/CN119343440A/zh active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| DE102022206019A1 (de) | 2023-12-14 |
| CN119343440A (zh) | 2025-01-21 |
| KR20250022699A (ko) | 2025-02-17 |
| JP2025519649A (ja) | 2025-06-26 |
| WO2023242148A1 (de) | 2023-12-21 |
| AU2023293665A1 (en) | 2024-12-12 |
| CA3257815A1 (en) | 2025-03-19 |
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