EP4486898A4 - Genetisch modifiziertes bakterium mit einem lysegen - Google Patents

Genetisch modifiziertes bakterium mit einem lysegen

Info

Publication number
EP4486898A4
EP4486898A4 EP23763899.4A EP23763899A EP4486898A4 EP 4486898 A4 EP4486898 A4 EP 4486898A4 EP 23763899 A EP23763899 A EP 23763899A EP 4486898 A4 EP4486898 A4 EP 4486898A4
Authority
EP
European Patent Office
Prior art keywords
lysegene
genetically modified
modified bacterium
bacterium
genetically
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP23763899.4A
Other languages
English (en)
French (fr)
Other versions
EP4486898A2 (de
Inventor
Nalinikanth Kotagiri
Jordan Kharofa
Nitin Kamble
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Cincinnati
Original Assignee
University of Cincinnati
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Cincinnati filed Critical University of Cincinnati
Publication of EP4486898A2 publication Critical patent/EP4486898A2/de
Publication of EP4486898A4 publication Critical patent/EP4486898A4/de
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2827—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against B7 molecules, e.g. CD80, CD86
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP23763899.4A 2022-03-01 2023-03-01 Genetisch modifiziertes bakterium mit einem lysegen Pending EP4486898A4 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202263315512P 2022-03-01 2022-03-01
PCT/US2023/014290 WO2023167936A2 (en) 2022-03-01 2023-03-01 Genetically modified bacterium including a lysis gene

Publications (2)

Publication Number Publication Date
EP4486898A2 EP4486898A2 (de) 2025-01-08
EP4486898A4 true EP4486898A4 (de) 2026-03-25

Family

ID=87884226

Family Applications (1)

Application Number Title Priority Date Filing Date
EP23763899.4A Pending EP4486898A4 (de) 2022-03-01 2023-03-01 Genetisch modifiziertes bakterium mit einem lysegen

Country Status (2)

Country Link
EP (1) EP4486898A4 (de)
WO (1) WO2023167936A2 (de)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023015441A1 (zh) * 2021-08-10 2023-02-16 中国科学院深圳先进技术研究院 一种光控裂解工程菌及其构建方法和应用

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100990186B1 (ko) * 2008-10-20 2010-10-29 주식회사 중앙백신연구소 수용성 ＡｐｘＩＩＡ 단백질 생산용 재조합 발현 벡터

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023015441A1 (zh) * 2021-08-10 2023-02-16 中国科学院深圳先进技术研究院 一种光控裂解工程菌及其构建方法和应用

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CHIH-HSIANG CHANG ET AL: "Engineering of Escherichia coli for targeted delivery of transgenes to HER2/neu-positive tumor cells", BIOTECHNOLOGY AND BIOENGINEERING, JOHN WILEY, HOBOKEN, USA, vol. 108, no. 7, 24 February 2011 (2011-02-24), pages 1662 - 1672, XP071113782, ISSN: 0006-3592, DOI: 10.1002/BIT.23095 *
FU SHENGWEI ET AL: "Programming the lifestyles of engineered bacteria for cancer therapy", NATIONAL SCIENCE REVIEW, vol. 10, no. 5, 14 February 2023 (2023-02-14), XP093363653, ISSN: 2095-5138, Retrieved from the Internet <URL:https://academic.oup.com/nsr/article-pdf/10/5/nwad031/49848248/nwad031.pdf> DOI: 10.1093/nsr/nwad031 *
GAO SHUANG ET AL: "Development of Oxytolerant Salmonella typhimurium Using Radiation Mutation Technology (RMT) for Cancer Therapy", SCIENTIFIC REPORTS, vol. 10, no. 1, 28 February 2020 (2020-02-28), US, XP093363650, ISSN: 2045-2322, Retrieved from the Internet <URL:https://www.nature.com/articles/s41598-020-60396-6> DOI: 10.1038/s41598-020-60396-6 *
KAMBLE ET AL.: "Radiolytic Escherichia coli Nissle: A novel radiosensitizer delivery platform using a live bacterial therapeutic (Abstract 114)", INTERNATIONAL JOURNAL OF RADIATION: ONCOLOGY BIOLOGY PHYSICS, PERGAMON PRESS, USA, vol. 114, no. 3, 22 October 2022 (2022-10-22), XP087206808, ISSN: 0360-3016, DOI: 10.1016/J.IJROBP.2022.07.351 *
XIE SONGZHI ET AL: "Bacterial navigation for tumor targeting and photothermally-triggered bacterial ghost transformation for spatiotemporal drug release", ACTA BIOMATERIALIA, ELSEVIER, AMSTERDAM, NL, vol. 131, 23 June 2021 (2021-06-23), pages 172 - 184, XP086747139, ISSN: 1742-7061, [retrieved on 20210623], DOI: 10.1016/J.ACTBIO.2021.06.030 *

Also Published As

Publication number Publication date
EP4486898A2 (de) 2025-01-08
WO2023167936A2 (en) 2023-09-07
WO2023167936A3 (en) 2023-10-19

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