EP4482617C0 - Verfahren zur erzeugung von arrays unter verwendung von mikrofluidik und photolithographie - Google Patents

Verfahren zur erzeugung von arrays unter verwendung von mikrofluidik und photolithographie

Info

Publication number
EP4482617C0
EP4482617C0 EP23745007.7A EP23745007A EP4482617C0 EP 4482617 C0 EP4482617 C0 EP 4482617C0 EP 23745007 A EP23745007 A EP 23745007A EP 4482617 C0 EP4482617 C0 EP 4482617C0
Authority
EP
European Patent Office
Prior art keywords
microfluidics
photolithography
producing arrays
arrays
producing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP23745007.7A
Other languages
English (en)
French (fr)
Other versions
EP4482617A1 (de
EP4482617B1 (de
Inventor
Preyas Shah
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
10X Genomics Inc
Original Assignee
10X Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 10X Genomics Inc filed Critical 10X Genomics Inc
Priority to EP25180565.1A priority Critical patent/EP4659854A1/de
Publication of EP4482617A1 publication Critical patent/EP4482617A1/de
Application granted granted Critical
Publication of EP4482617C0 publication Critical patent/EP4482617C0/de
Publication of EP4482617B1 publication Critical patent/EP4482617B1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/0054—Means for coding or tagging the apparatus or the reagents
    • B01J2219/00547—Bar codes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00608—DNA chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
EP23745007.7A 2022-06-29 2023-06-28 Verfahren zur erzeugung von arrays unter verwendung von mikrofluidik und photolithographie Active EP4482617B1 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP25180565.1A EP4659854A1 (de) 2022-06-29 2023-06-28 Verfahren zur erzeugung von arrays unter verwendung von mikrofluidik und photolithographie

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202263356735P 2022-06-29 2022-06-29
PCT/US2023/069241 WO2024006814A1 (en) 2022-06-29 2023-06-28 Method of generating arrays using microfluidics and photolithography

Related Child Applications (2)

Application Number Title Priority Date Filing Date
EP25180565.1A Division EP4659854A1 (de) 2022-06-29 2023-06-28 Verfahren zur erzeugung von arrays unter verwendung von mikrofluidik und photolithographie
EP25180565.1A Division-Into EP4659854A1 (de) 2022-06-29 2023-06-28 Verfahren zur erzeugung von arrays unter verwendung von mikrofluidik und photolithographie

Publications (3)

Publication Number Publication Date
EP4482617A1 EP4482617A1 (de) 2025-01-01
EP4482617C0 true EP4482617C0 (de) 2025-08-06
EP4482617B1 EP4482617B1 (de) 2025-08-06

Family

ID=87429134

Family Applications (2)

Application Number Title Priority Date Filing Date
EP23745007.7A Active EP4482617B1 (de) 2022-06-29 2023-06-28 Verfahren zur erzeugung von arrays unter verwendung von mikrofluidik und photolithographie
EP25180565.1A Pending EP4659854A1 (de) 2022-06-29 2023-06-28 Verfahren zur erzeugung von arrays unter verwendung von mikrofluidik und photolithographie

Family Applications After (1)

Application Number Title Priority Date Filing Date
EP25180565.1A Pending EP4659854A1 (de) 2022-06-29 2023-06-28 Verfahren zur erzeugung von arrays unter verwendung von mikrofluidik und photolithographie

Country Status (3)

Country Link
US (1) US20240076721A1 (de)
EP (2) EP4482617B1 (de)
WO (1) WO2024006814A1 (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025101377A1 (en) 2023-11-09 2025-05-15 10X Genomics, Inc. Matrix-assisted spatial analysis of biological samples

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5472881A (en) 1992-11-12 1995-12-05 University Of Utah Research Foundation Thiol labeling of DNA for attachment to gold surfaces
US5610287A (en) 1993-12-06 1997-03-11 Molecular Tool, Inc. Method for immobilizing nucleic acid molecules
US5807522A (en) 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
EP2256133B1 (de) 1997-01-08 2016-12-14 Sigma-Aldrich Co. LLC Biokonjugation von Makromolekülen
US5837860A (en) 1997-03-05 1998-11-17 Molecular Tool, Inc. Covalent attachment of nucleic acid molecules onto solid-phases via disulfide bonds
US7427678B2 (en) 1998-01-08 2008-09-23 Sigma-Aldrich Co. Method for immobilizing oligonucleotides employing the cycloaddition bioconjugation method
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
EP2226316B1 (de) 2002-05-30 2016-01-13 The Scripps Research Institute Kupferkatalysierte Ligierung von Aziden und Acetylenen
US7259258B2 (en) 2003-12-17 2007-08-21 Illumina, Inc. Methods of attaching biological compounds to solid supports using triazine
EP2789383B1 (de) 2004-01-07 2023-05-03 Illumina Cambridge Limited Molekül-Arrays
GB0427236D0 (en) 2004-12-13 2005-01-12 Solexa Ltd Improved method of nucleotide detection
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
DK3511423T4 (da) 2012-10-17 2024-07-29 Spatial Transcriptomics Ab Fremgangsmåder og produkt til optimering af lokaliseret eller rumlig detektion af genekspression i en vævsprøve
EP3280820B1 (de) 2015-04-10 2019-06-26 Spatial Transcriptomics AB Räumlich getrennte multiplex-nukleinsäureanalyse von biologischen proben
GB201619458D0 (en) 2016-11-17 2017-01-04 Spatial Transcriptomics Ab Method for spatial tagging and analysing nucleic acids in a biological specimen
EP3826762A1 (de) * 2018-07-23 2021-06-02 DNA Script Massiv parallele enzymatische synthese von nukleinsäuresträngen
US12415171B2 (en) * 2018-12-21 2025-09-16 Microsoft Technology Licensing, Llc Regulation of DNA synthesis by nucleotides linked to protecting groups
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
FI3891300T3 (fi) 2019-12-23 2023-05-10 10X Genomics Inc Menetelmät spatiaalista analyysiä varten rna-templatoitua ligaatiota käyttäen
CA3172763A1 (en) * 2020-02-19 2021-08-26 Polymer Forge, Inc. Microarrays
EP4553168A3 (de) 2020-05-22 2025-08-27 10x Genomics, Inc. Räumliche analyse zur erkennung von sequenzvarianten
US12391970B2 (en) * 2020-11-11 2025-08-19 Microsoft Technology Licensing, Llc Spatial control of polynucleotide synthesis by strand capping
WO2022147140A1 (en) 2020-12-30 2022-07-07 10X Genomics, Inc. Molecular array generation using photoresist
WO2022147139A1 (en) 2020-12-30 2022-07-07 10X Genomics, Inc. Methods and compositions for light-controlled surface patterning using a polymer
US20220228201A1 (en) 2020-12-30 2022-07-21 10X Genomics, Inc. Molecular arrays and methods for generating and using the arrays

Also Published As

Publication number Publication date
EP4659854A1 (de) 2025-12-10
WO2024006814A1 (en) 2024-01-04
EP4482617A1 (de) 2025-01-01
US20240076721A1 (en) 2024-03-07
EP4482617B1 (de) 2025-08-06

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