EP4240848A4 - Neuartige omni-50-crispr-nuklease-rna-komplexe - Google Patents

Neuartige omni-50-crispr-nuklease-rna-komplexe

Info

Publication number
EP4240848A4
EP4240848A4 EP21889943.3A EP21889943A EP4240848A4 EP 4240848 A4 EP4240848 A4 EP 4240848A4 EP 21889943 A EP21889943 A EP 21889943A EP 4240848 A4 EP4240848 A4 EP 4240848A4
Authority
EP
European Patent Office
Prior art keywords
rna complexes
crispr nuclease
novel omni
nuclease rna
omni
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP21889943.3A
Other languages
English (en)
French (fr)
Other versions
EP4240848A1 (de
Inventor
Lior Izhar
Liat Rockah
Bar Nadav Marbach
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Emendobio Inc
Original Assignee
Emendobio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Emendobio Inc filed Critical Emendobio Inc
Publication of EP4240848A1 publication Critical patent/EP4240848A1/de
Publication of EP4240848A4 publication Critical patent/EP4240848A4/de
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C12N9/222—Clustered regularly interspaced short palindromic repeats [CRISPR]-associated [CAS] enzymes
    • C12N9/226—Class 2 CAS enzyme complex, e.g. single CAS protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
EP21889943.3A 2020-11-04 2021-11-03 Neuartige omni-50-crispr-nuklease-rna-komplexe Pending EP4240848A4 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202063109835P 2020-11-04 2020-11-04
PCT/US2021/057814 WO2022098693A1 (en) 2020-11-04 2021-11-03 Novel omni-50 crispr nuclease-rna complexes

Publications (2)

Publication Number Publication Date
EP4240848A1 EP4240848A1 (de) 2023-09-13
EP4240848A4 true EP4240848A4 (de) 2025-10-01

Family

ID=81457398

Family Applications (1)

Application Number Title Priority Date Filing Date
EP21889943.3A Pending EP4240848A4 (de) 2020-11-04 2021-11-03 Neuartige omni-50-crispr-nuklease-rna-komplexe

Country Status (5)

Country Link
US (1) US20240002840A1 (de)
EP (1) EP4240848A4 (de)
JP (1) JP2023549139A (de)
CN (1) CN116670271A (de)
WO (1) WO2022098693A1 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12091688B2 (en) 2021-02-08 2024-09-17 Emendobio Inc. OMNI-103 CRISPR nuclease
EP4288085A4 (de) 2021-02-08 2025-01-29 Emendobio Inc. Omni103-crispr-nuklease
EP4662307A1 (de) * 2023-02-08 2025-12-17 Emendobio Inc. Manipulierte omni-50-nukleasevarianten

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20200190542A1 (en) * 2012-05-25 2020-06-18 The Regents Of The University Of California Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription
WO2020223514A2 (en) * 2019-04-30 2020-11-05 Emendobio Inc. Novel omni-50 crispr nuclease
WO2020223553A2 (en) * 2019-04-30 2020-11-05 Emendobio Inc. Novel omni crispr nucleases

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3957735A1 (de) * 2014-03-05 2022-02-23 Editas Medicine, Inc. Verfahren im zusammenhang mit crispr/cas und verfahren zur behandlung des usher-syndroms und von retinitis pigmentosa
CN105567735A (zh) * 2016-01-05 2016-05-11 华东师范大学 一种凝血因子基因突变的定点修复载体系统及方法
KR20200124702A (ko) * 2018-02-23 2020-11-03 파이어니어 하이 부렛드 인터내쇼날 인코포레이팃드 신규한 cas9 오르소로그
WO2020069029A1 (en) * 2018-09-26 2020-04-02 Emendobio Inc. Novel crispr nucleases
WO2020214619A1 (en) * 2019-04-15 2020-10-22 Emendobio Inc. Crispr compositions and methods for promoting gene editing of gata2

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20200190542A1 (en) * 2012-05-25 2020-06-18 The Regents Of The University Of California Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription
WO2020223514A2 (en) * 2019-04-30 2020-11-05 Emendobio Inc. Novel omni-50 crispr nuclease
WO2020223553A2 (en) * 2019-04-30 2020-11-05 Emendobio Inc. Novel omni crispr nucleases

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DATABASE Geneseq [online] 24 December 2020 (2020-12-24), "Ezakiella peruensis strain M6.X2 CRISPR nuclease, SEQ ID 3.", XP002813765, retrieved from EBI accession no. GSP:BIN56603 Database accession no. BIN56603 *
DATABASE Geneseq [online] 24 December 2020 (2020-12-24), "Ezakiella peruensis strain M6.X2 CRISPR nuclease, SEQ ID 3.", XP002813767, retrieved from EBI accession no. GSP:BIN48917 Database accession no. BIN48917 *
DATABASE Geneseq [online] 24 December 2020 (2020-12-24), "Genome editing related RNA, SEQ ID 45.", XP002813768, retrieved from EBI accession no. GSN:BIN48959 Database accession no. BIN48959 *
DATABASE Geneseq [online] 24 December 2020 (2020-12-24), "Human ELANE gene-targeted sgRNA related scaffold, SEQ ID 45.", XP002813766, retrieved from EBI accession no. GSN:BIN56645 Database accession no. BIN56645 *
DATABASE Geneseq [online] 6 August 2020 (2020-08-06), "Peptoniphilus duerdenii CRISPR-associated 9 (Cas9)/Csn1, SEQ ID 163.", XP002813769, retrieved from EBI accession no. GSP:BHW50185 Database accession no. BHW50185 *
See also references of WO2022098693A1 *

Also Published As

Publication number Publication date
EP4240848A1 (de) 2023-09-13
WO2022098693A1 (en) 2022-05-12
US20240002840A1 (en) 2024-01-04
JP2023549139A (ja) 2023-11-22
CN116670271A (zh) 2023-08-29

Similar Documents

Publication Publication Date Title
EP3820495A4 (de) Rna-programmierbare epigenetische rna-modifikatoren und deren verwendungen
IL288956A (en) RN" A building
EP3914259A4 (de) Rna-editierende oligonukleotide und deren verwendung
EP3914260A4 (de) Rna-editierende oligonukleotide und deren verwendung
IL271342A (en) Nucleic acid-guided nucleases
IL304263A (en) RNA structure with self-circulation
EP3359644A4 (de) Neuartige rna-geführte nukleasen und verwendungen davon
MA49421A (fr) Formulations d'arn
EP3963061A4 (de) Neue omni-50-crispr-nuklease
EP3668998C0 (de) Rna-template-ligation
SG11201708653RA (en) Evaluation of cas9 molecule/guide rna molecule complexes
PL3507366T3 (pl) Chemicznie modyfikowane jednoniciowe oligonukleotydy edytujące rna
LT3433368T (lt) Trans-replikuojama rnr
EP4240848A4 (de) Neuartige omni-50-crispr-nuklease-rna-komplexe
EP2946015A4 (de) Cas9-nukleinsäurekomplexe und verwendungen dafür
EP3436581A4 (de) Verfahren von gesamttranskriptomamplifikation
EP3938136C0 (de) Hairpin-schweissverfahren
EP3749350A4 (de) Genetisch manipulierte zielspezifische nukleasen
EP3475449A4 (de) Zellfreie nukleinsäurestandards und verwendungen davon
IL311976A (en) RNA molecules
EP3999639A4 (de) Stabilisierte crispr-komplexe
EP3401031C0 (de) Verbesserte biegemaschine
EP3740577A4 (de) Immunomodulatorische small-hairpin-rna-moleküle
IL309943A (en) Signal activatable nucleic acid complexes
IL313397A (en) Omni-103 crispr nuclease-rna complexes

Legal Events

Date Code Title Description
STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE

PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE

17P Request for examination filed

Effective date: 20230601

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

DAV Request for validation of the european patent (deleted)
DAX Request for extension of the european patent (deleted)
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 40101089

Country of ref document: HK

A4 Supplementary search report drawn up and despatched

Effective date: 20250901

RIC1 Information provided on ipc code assigned before grant

Ipc: C12N 15/11 20060101AFI20250826BHEP

Ipc: C12N 9/22 20060101ALI20250826BHEP

Ipc: A61K 48/00 20060101ALI20250826BHEP