EP4212841A1 - Apparatus for determining the temperature of at least one fluid - Google Patents
Apparatus for determining the temperature of at least one fluid Download PDFInfo
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- EP4212841A1 EP4212841A1 EP22151428.4A EP22151428A EP4212841A1 EP 4212841 A1 EP4212841 A1 EP 4212841A1 EP 22151428 A EP22151428 A EP 22151428A EP 4212841 A1 EP4212841 A1 EP 4212841A1
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- 239000012530 fluid Substances 0.000 title claims abstract description 108
- 239000000835 fiber Substances 0.000 claims abstract description 105
- 230000003287 optical effect Effects 0.000 claims abstract description 102
- 239000013307 optical fiber Substances 0.000 claims abstract description 44
- 230000001427 coherent effect Effects 0.000 claims abstract description 25
- 238000012545 processing Methods 0.000 claims abstract description 22
- 238000000034 method Methods 0.000 claims description 17
- 238000005259 measurement Methods 0.000 description 11
- 238000012360 testing method Methods 0.000 description 10
- 230000010363 phase shift Effects 0.000 description 8
- 238000003752 polymerase chain reaction Methods 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 230000006870 function Effects 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- 238000001514 detection method Methods 0.000 description 3
- 230000002238 attenuated effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 230000000737 periodic effect Effects 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 238000003491 array Methods 0.000 description 1
- 238000007705 chemical test Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000011152 fibreglass Substances 0.000 description 1
- 231100001261 hazardous Toxicity 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000010183 spectrum analysis Methods 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01K—MEASURING TEMPERATURE; MEASURING QUANTITY OF HEAT; THERMALLY-SENSITIVE ELEMENTS NOT OTHERWISE PROVIDED FOR
- G01K11/00—Measuring temperature based upon physical or chemical changes not covered by groups G01K3/00, G01K5/00, G01K7/00 or G01K9/00
- G01K11/32—Measuring temperature based upon physical or chemical changes not covered by groups G01K3/00, G01K5/00, G01K7/00 or G01K9/00 using changes in transmittance, scattering or luminescence in optical fibres
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01K—MEASURING TEMPERATURE; MEASURING QUANTITY OF HEAT; THERMALLY-SENSITIVE ELEMENTS NOT OTHERWISE PROVIDED FOR
- G01K11/00—Measuring temperature based upon physical or chemical changes not covered by groups G01K3/00, G01K5/00, G01K7/00 or G01K9/00
- G01K11/32—Measuring temperature based upon physical or chemical changes not covered by groups G01K3/00, G01K5/00, G01K7/00 or G01K9/00 using changes in transmittance, scattering or luminescence in optical fibres
- G01K11/3206—Measuring temperature based upon physical or chemical changes not covered by groups G01K3/00, G01K5/00, G01K7/00 or G01K9/00 using changes in transmittance, scattering or luminescence in optical fibres at discrete locations in the fibre, e.g. using Bragg scattering
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01D—MEASURING NOT SPECIALLY ADAPTED FOR A SPECIFIC VARIABLE; ARRANGEMENTS FOR MEASURING TWO OR MORE VARIABLES NOT COVERED IN A SINGLE OTHER SUBCLASS; TARIFF METERING APPARATUS; MEASURING OR TESTING NOT OTHERWISE PROVIDED FOR
- G01D5/00—Mechanical means for transferring the output of a sensing member; Means for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for converting; Transducers not specially adapted for a specific variable
- G01D5/26—Mechanical means for transferring the output of a sensing member; Means for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for converting; Transducers not specially adapted for a specific variable characterised by optical transfer means, i.e. using infrared, visible, or ultraviolet light
- G01D5/268—Mechanical means for transferring the output of a sensing member; Means for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for converting; Transducers not specially adapted for a specific variable characterised by optical transfer means, i.e. using infrared, visible, or ultraviolet light using optical fibres
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
- G01N21/45—Refractivity; Phase-affecting properties, e.g. optical path length using interferometric methods; using Schlieren methods
Definitions
- the present invention relates to an apparatus for determining the temperature of at least one fluid, and in particular, to an apparatus for determining the temperature of at least one fluid with increased accuracy, wherein the apparatus comprises an optical fiber.
- PCR polymerase chain reaction
- PCR tests it is known to not directly monitor the temperature, but to monitor the temperature of a heating plate on which the temperature of the corresponding fluid depends. Further, it is known to use metallic resistance sensors to measure the temperature of a fluid, wherein, however, metallic resistance sensors are usually large and can have a probe diameter of at least 1mm, which is not suitable for measuring or determining the temperature of a small amount of fluid.
- a fiber optic cable is an assembly similar to an electrical cable but containing one or more optical fibers used to carry light.
- Apparatuses for monitoring or determining a temperature that comprise a fiber optic or fiber-optic temperature sensors, respectively, are commonly used in given applications as an advantageous alternative to thermocouples and the like.
- Fiber-optic temperature sensors are immune to electromagnetic interference or radio-frequency interference, respectively.
- fiber-optic temperature sensors are relatively small and can withstand hazardous environments, including relatively extreme temperatures.
- apparatuses for monitoring or determining the temperature of a fluid that comprises an optical fiber are known that are based on fiber bragg gratings or arrays thereof, which are, however, usually longer than 10mm and require a complex spectral analysis for interrogation.
- Other known solutions require a complex interrogation, for example fiber modal interferometers, or have a complex measurement setup, for example apparatuses that comprise a liquid filled fiber. Therefore, there is a need for an improved apparatus for determining the temperature of at least one fluid that comprises a fiber optic.
- the document US 5,231,681 A discloses an optical fibre cable for detecting a change in temperature, said fibre cable including an elongated temperature-responsive device and an optical fibre in abutment with said temperature-responsive device and fastened thereto by means of a fastening device which embraces the fibre and the temperature-responsive device, wherein a light pulse transmitted into a non-influenced fibre cable from one end thereof is attenuated generally uniformly along the length of the fibre and wherein the light pulse is further attenuated when the optical fibre is bent by the temperature-responsive device, wherein the temperature-responsive device includes an elastic tube which contains a detector material whose coefficient of cubic expansion is within a temperature detecting range, in which said detector material changes from one phase to another phase, and has a value which exceeds the value of the coefficient of cubic expansion in adjacent temperature ranges.
- this object is solved by an apparatus for determining the temperature of at least one fluid, wherein the apparatus comprises an optical fiber, wherein a first end of the optical fiber is connected to at least one fiber tip, and a first additional reflector introduced into the at least one fiber tip at a first predetermined distance from an outer end of the at least one fiber tip, and wherein a second end of the optical fiber is connected to a processing apparatus, wherein the processing apparatus comprises an optical source, wherein the optical source is configured to launch an optical signal into the optical fiber, and a coherent detector, wherein the coherent detector is configured to determine the temperature of at least one fluid by receiving a first light signal that corresponds to parts of the optical signal that are reflected at the outer end of the at least one fiber tip when the at least one fiber tip is inserted into the at least one fluid and a second light signal that corresponds to parts of the optical signal that are reflected at the first additional reflector when the at least one fiber tip is inserted into the at least one fluid, determining a difference of the optical phases of
- a coherent detector is a detector in which both the phase and the amplitude of the received signals are used and analyzed.
- Coherent detectors usually have a high sensitivity and are also suitable to detect or determine the phase change.
- a first additional reflector being introduced into the at least one fiber tip at a first predetermined distance from an outer end of the at least one fiber tip or an end of the fiber tip opposite to an end of the fiber tip that is connected to the optical fiber means that a reference reflection is introduced to define a predetermined or desired measurement section.
- phase shift can be defined as a function of the temperature.
- an improved apparatus for determining the temperature of at least one fluid is provided.
- the apparatus further comprises a second additional reflector that is introduced in the at least one fiber tip at a second predetermined distance from the first additional reflector.
- a second additional reflector being introduced into the at least one fiber tip at a second predetermined distance from the first additional reflectometer means that a further, second reference reflection is introduced to define a further predefined or desired measurement selection.
- this second additional reflector can, for example, be used as additional reflection with shorter distance to increase a range of unique measurements.
- the coherent detector can be configured to determine the temperature of the at least one fluid by receiving the first light signal, the second light signal and a third light signal that corresponds to parts of the optical signal that are reflected at the second additional reflector when the at least one fiber tip is inserted into the fluid, by determining a difference of the optical phases of the first light signal and the second light signal and a difference of the optical phases of the second light signal and the third light signal, and by determining the temperature of the at least one fluid based on the difference of the optical phases of the first light signal and the second light signal and the difference of the optical phases of the second light signal and the third light signal.
- the temperature of the at least one fluid being determined based on the difference of the optical phases of the first light signal and the second light signal and the difference of the optical phases of the second light signal and the third light signal can for example mean that first, an approximate value of the temperature can be determined based on the difference of the optical phases of the first light signal and the second light signal, wherein, thereafter, a more exact value of the temperature can be determined based on the difference of the optical phases of the second light signal and the third light signal, and wherein the first predetermined distance is greater than the first predetermined distance.
- the determining range can be optimized by simultaneously using two different measurement sections, wherein the determining range can be increased based on a first phase change, and wherein, at the same time, also the accuracy of the determined temperature can be further improved based on a second phase change.
- the apparatus comprises a plurality of fiber tips, wherein the apparatus is configured in such a way that all fiber tips can simultaneously be inserted into a plurality of fluids in such a way that each of the plurality of fiber tips is inserted in a different one of the plurality of fluids, and wherein the processing apparatus is configured to determine the temperature of each of the plurality of fluids.
- multiple fiber tips can be used in parallel, thereby increasing the capacities of a corresponding test, wherein the signals of the plurality of fiber tips can be multiplexed, for example time multiplexed via delay lines, and wherein the signal processor can be programmed in such a way that it respectively knows when the signals of each of the plurality of fiber tips are processed.
- the processing apparatus can be a coherent optical time domain reflectometer.
- An optical domain reflectometer periodically transmits a detection pulse to an optical fiber.
- the detection pulse will have Fresnel reflection at certain points in the fiber, where the refractive index changes or imperfections are introduced in the fiber.
- the Fresnel reflection signal from the optical signal is then received and analyzed.
- a coherent optical time domain reflectometer (COTDR) further works by means of coherent detection technology.
- coherent optical time domain reflectometers have an improved sensitivity and are suitable to detect and determine a phase change, for example the difference of the optical phases of the first light signal and the second light signal.
- a system for determining the temperature of at least one fluid comprising at least one fluid reservoir and an apparatus for determining the temperature of at least one fluid as described above, wherein the apparatus for determining the temperature of at least one fluid is configured to determine the temperature of at least one fluid contained in the at least one fluid reservoir.
- a system for determining the temperature of at least one fluid is provided that is based on an improved apparatus for determining the temperature of at least one fluid.
- the system is based on an apparatus that takes into account that, when an optical signal, for example light, propagates in a medium, for example an optical fiber, the light wave undergoes a phase shift, wherein the phase shift can be defined as a function of the temperature.
- an optical signal for example light
- the phase shift can be defined as a function of the temperature.
- a method for determining the temperature of at least one fluid comprises inserting at least one fiber tip into at least one fluid, wherein the fiber tip is connected to an optical fiber, and wherein a first additional reflector is introduced into the at least one fiber tip at a first predetermined distance from an outer end of the at least one fiber tip, and determining the temperature of the at least one fluid through a processing apparatus that is connected to a second end of the optical fiber by launching an optical signal into the optical fiber, receiving a first light signal that corresponds to parts of the optical signal that are reflected at the outer end of the at least one fiber tip and a second light signal that corresponds to parts of the optical signal that are reflected at the first additional reflector, determining a difference of the optical phases of the first light signal and the second light signal, and determining the temperature of the at least one fluid based on the difference of the optical phases of the first light signal and the second light signal.
- an improved method for determining the temperature of at least one fluid takes into account that, when an optical signal, for example light, propagates in a medium, for example an optical fiber, the light wave undergoes a phase shift, wherein the phase shift can be defined as a function of the temperature.
- the accuracy of the determination of the temperature can be increased without having to costly amend the apparatus as such.
- a second additional reflector is introduced in the at least one fiber tip at a second predetermined distance from the first additional reflector
- the step of determining the temperature of the at least one fluid through the processing apparatus comprises launching an optical signal into the optical fiber, receiving the first light signal, the second light signal and a third light signal that corresponds to parts of the optical signal that are reflected at the second additional reflector when the at least one fiber tip is inserted into the fluid, determining a difference of the optical phases of the first light signal and the second light signal and a difference of the optical phases of the second light signal and the third light signal, and determining the temperature of the at least one fluid based on the difference of the optical phases of the first light signal and the second light signal and the difference of the optical phases of the second light signal and the third light signal.
- the determining range can be optimized by simultaneously using two different measurement sections, wherein the determining range can be increased based on a first phase change, and wherein, at the same time, also the accuracy of the determined temperature can be further improved based on a second phase change.
- a plurality of fiber tips are simultaneously inserted into a plurality of fluids in such a way that each of the plurality of fiber tips is inserted in a different one of the plurality of fluids, and wherein the temperature of each of the plurality of fluids is determined by the processing apparatus.
- multiple fiber tips can be used in parallel, thereby increasing the capacities of a corresponding test, wherein the signals of the plurality of fiber tips can be multiplexed, for example time multiplexed via delay lines, and wherein the signal processor can be programmed in such a way that it respectively knows when the signals of each of the plurality of fiber tips are processed.
- the processing apparatus includes a coherent detector.
- Coherent detectors usually have a high sensitivity and are suitable to detect or determine the phase change.
- the processing apparatus can be a coherent optical time domain reflectometer.
- coherent optical time domain reflectometers have an improved sensitivity and are suitable to detect and determine a phase change, for example the difference of the optical phases of the first light signal and the second light signal.
- Fig. 1 illustrates an apparatus for determining the temperature of at least one fluid 1 according to embodiments of the invention.
- the temperature of a small amount of fluid needs to be measured.
- it can be required to monitor the temperature change which accompanies a reaction.
- the accuracy and precision of the apparatus that is used to monitor the temperature are one of the main factors which affect such measurement.
- a small amount of fluid for example 100 ⁇ l, is usually inserted into small wells, wherein a plurality of wells can be contained in a common plate, and wherein each well usually has a depth of approximately only 20mm.
- an apparatus for determining the temperature of at least one fluid 1 comprising an optical fiber 2, wherein a first end of the optical fiber 3 is connected to at least one fiber tip 4, and a first additional reflector 5 introduced into the at least one fiber tip 3 at a first predetermined distance from an outer end of the at least one fiber tip 6, and wherein a second end of the optical fiber 7, opposite the first end of the fiber tip 3, is connected to a processing apparatus 8, wherein the processing apparatus 8 comprises an optical source 9, wherein the optical source 9 is configured to launch an optical signal into the optical fiber 2, and a coherent detector 10, wherein the coherent detector 10 is configured to determine the temperature of at least one fluid and comprises a receiving device 11 that is configured to receive a first light signal that corresponds to parts of the optical signal that are reflected at the outer end of the at least one fiber tip 6 when the at least one fiber tip 4 is inserted into the at least one fluid and a second light signal that corresponds to parts of the optical signal that are reflected at the
- phase shift can be defined as a function of the temperature.
- an improved apparatus for determining the temperature of at least one fluid 1 is provided.
- the receiving device can for example be realized by a signal receiver.
- the first determining device and the second determining device can, for example, further be realized by code, that is stored in a memory and executable by a processor.
- a bare fiber tip which has a length of approximately 10mm, is used to probe the temperature of the fluid, which changes the refractive index of the corresponding fiber glass.
- the at least one fiber tip has been produced by cleaving the tip of the optical fiber 2 and removing the coating of the optical fiber over a section of about 10mm.
- the first predetermined distance is approximately 10mm, wherein the first additional reflector 5 has been introduced close to a transition between the at least one fiber tip 4 and the optical fiber 2, or close to the first end of the optical fiber 3, respectively, wherein the first additional reflector can for example be introduced by splicing, UV inscription, scrimping, e-beam writing, or any other known suitable method.
- the corresponding phase change in the at least one fiber tip 4 is used as a measure for the temperature change based on the first additional reflector as measurement section.
- optical source is configured in such a way that reflected signals from the fiber tip can be distinguished from additional reflection.
- the apparatus 1 further comprises a second additional reflector 14 that is introduced in the at least one fiber tip 4 at a predetermined distance from the first additional reflector.
- the phase change over 10mm and a temperature change of 8K is approximately 2 ⁇ and therefore, periodic over the temperature, wherefore in this case, the temperature determination is not unique anymore.
- a second smaller reflection is introduced into the fiber tip 4, wherein, according to the embodiments of Fig. 1 , this second additional reflector 14 is introduced into the fiber tip 4 approximately 1mm after the first additional reflector 5, and wherein the second additional reflector can again, for example, be introduced by splicing, UV inscription, scrimping, e-beam writing, or any other known suitable method.
- the second additional reflector 14 is used to increase the range of unique measurements.
- the processing apparatus 8 is configured to determine the temperature of the at least one fluid by launching an optical signal into the optical fiber, wherein the receiving device 11 is configured to receive the first light signal, the second light signal and a third light signal that corresponds to parts of the optical signal that are reflected at the second additional reflector 14 when the at least one fiber tip 4 is inserted into the fluid, wherein the first determining device 12 is configured to determine a difference of the optical phases of the first light signal and the second light signal and a difference of the optical phases of the second light signal and the third light signal, and wherein the second determining device 13 is configured to determine the temperature of the at least one fluid based on the difference of the optical phases of the first light signal and the second light signal and the difference of the optical phases of the second light signal and the third light signal.
- the apparatus can also comprise a plurality of fiber tips, wherein the apparatus can be configured in such a way that all fiber tips can simultaneously be inserted into a plurality of fluids in such a way that each of the plurality of fiber tips is inserted in a different one of the plurality of fluids, and wherein the processing apparatus is configured to determine the temperature of each of the plurality of fluids. For example, this can be achieved by using a 1xN fiber coupler and lead fibers and/or time-multiplexing the signals from the individual fiber tips via delay lines.
- the processing apparatus 8 is further a coherent optical time domain reflectometer 15.
- the temperature of a small fluid amount of for example 100 ⁇ l can be determined with a small fiber tip 4 without interfering with the temperature of the fluid, wherein a high measurement or determination speed, respectively, is enabled.
- Fig. 2 illustrates a flowchart of a method for determining the temperature of at least one fluid 20 according to embodiments of the invention.
- the method 20 comprises a step 21 of inserting at least one fiber tip into at least one fluid, wherein the fiber tip is connected to an optical fiber, and wherein an additional first reflector is introduced into the at least one fiber tip at a first predetermined distance from an outer end of the at least one fiber tip, and a step 22 of determining the temperature of the at least one fluid, wherein the step 22 of determining the temperature of the at least one fluid comprises a step 23 of launching an optical signal into the optical fiber, a step 24 of receiving a first light signal that corresponds to parts of the optical signal that are reflected at the outer end of the at least one fiber tip and a second light signal that corresponds to parts of the optical signal that are reflected at the first additional reflector, a step 25 of determining a difference of the optical phases of the first light signal and the second light signal, and a step 26 of determining the temperature of the at least one fluid based on the difference of the optical phases of the first light signal and the second light signal.
- a second additional reflector is introduced into the at least one fiber tip at a second predetermined distance from the first additional reflectometer, wherein in step 24, the first light signal, the second light signal and a third light signal that corresponds to parts of the optical signal that are reflected at the second additional reflector when the at least one fiber tip is inserted into the fluid are received, wherein in step 25, a difference of the optical phases of the first light signal and the second light signal and a difference of the optical phases of the second light signal and the third light signal is determined, and wherein in step 26, the temperature of the at least one fluid is determined based on the difference of the optical phases of the first light signal and the second light signal and the difference of the optical phases of the second light signal and the third light signal.
- a plurality of fiber tips are simultaneously inserted into a plurality of fluids in step 21 in such a way that each of the plurality of fiber tips is inserted in a different one of the plurality of fluids, and wherein the method 20 further comprises determining the temperature of each of the plurality of fluids, or wherein step 23 of determining the temperature of the at least one fluid is separately executed for each of the wells, respectively.
- the method for determining the temperature of at least one fluid 20 shown in Fig. 2 can for example be used to monitor or determine the temperature during a biological or chemical test.
Abstract
Description
- The present invention relates to an apparatus for determining the temperature of at least one fluid, and in particular, to an apparatus for determining the temperature of at least one fluid with increased accuracy, wherein the apparatus comprises an optical fiber.
- During chemical or biological tests, for example a PCR (polymerase chain reaction) test, the temperature of a small amount of fluid needs to be measured. For example, during chemical or biological tests it can be required to monitor the temperature change which accompanies a reaction. Therein, the accuracy and precision of the apparatus that is used to monitor the temperature are one of the main factors which affect such measurement.
- Regarding for example PCR tests, it is known to not directly monitor the temperature, but to monitor the temperature of a heating plate on which the temperature of the corresponding fluid depends. Further, it is known to use metallic resistance sensors to measure the temperature of a fluid, wherein, however, metallic resistance sensors are usually large and can have a probe diameter of at least 1mm, which is not suitable for measuring or determining the temperature of a small amount of fluid.
- In order to save costs, there have also been attempts to monitor the temperature of fluids based on fiber optics or fiber optic cables. A fiber optic cable is an assembly similar to an electrical cable but containing one or more optical fibers used to carry light. Apparatuses for monitoring or determining a temperature that comprise a fiber optic or fiber-optic temperature sensors, respectively, are commonly used in given applications as an advantageous alternative to thermocouples and the like. Fiber-optic temperature sensors are immune to electromagnetic interference or radio-frequency interference, respectively. Moreover, fiber-optic temperature sensors are relatively small and can withstand hazardous environments, including relatively extreme temperatures.
- For example, apparatuses for monitoring or determining the temperature of a fluid that comprises an optical fiber are known that are based on fiber bragg gratings or arrays thereof, which are, however, usually longer than 10mm and require a complex spectral analysis for interrogation. Other known solutions require a complex interrogation, for example fiber modal interferometers, or have a complex measurement setup, for example apparatuses that comprise a liquid filled fiber. Therefore, there is a need for an improved apparatus for determining the temperature of at least one fluid that comprises a fiber optic.
- The document
US 5,231,681 A discloses an optical fibre cable for detecting a change in temperature, said fibre cable including an elongated temperature-responsive device and an optical fibre in abutment with said temperature-responsive device and fastened thereto by means of a fastening device which embraces the fibre and the temperature-responsive device, wherein a light pulse transmitted into a non-influenced fibre cable from one end thereof is attenuated generally uniformly along the length of the fibre and wherein the light pulse is further attenuated when the optical fibre is bent by the temperature-responsive device, wherein the temperature-responsive device includes an elastic tube which contains a detector material whose coefficient of cubic expansion is within a temperature detecting range, in which said detector material changes from one phase to another phase, and has a value which exceeds the value of the coefficient of cubic expansion in adjacent temperature ranges. - It is an object of the present application to provide an improved apparatus for determining the temperature of at least one fluid.
- According to one embodiment of the invention, this object is solved by an apparatus for determining the temperature of at least one fluid, wherein the apparatus comprises an optical fiber, wherein a first end of the optical fiber is connected to at least one fiber tip, and a first additional reflector introduced into the at least one fiber tip at a first predetermined distance from an outer end of the at least one fiber tip, and wherein a second end of the optical fiber is connected to a processing apparatus, wherein the processing apparatus comprises an optical source, wherein the optical source is configured to launch an optical signal into the optical fiber, and a coherent detector, wherein the coherent detector is configured to determine the temperature of at least one fluid by receiving a first light signal that corresponds to parts of the optical signal that are reflected at the outer end of the at least one fiber tip when the at least one fiber tip is inserted into the at least one fluid and a second light signal that corresponds to parts of the optical signal that are reflected at the first additional reflector when the at least one fiber tip is inserted into the at least one fluid, determining a difference of the optical phases of the first light signal and the second light signal, and determining the temperature of the at least one fluid based on the difference of the optical phases of the first light signal and the second light signal.
- Here, a coherent detector is a detector in which both the phase and the amplitude of the received signals are used and analyzed. Coherent detectors usually have a high sensitivity and are also suitable to detect or determine the phase change.
- Therein, a first additional reflector being introduced into the at least one fiber tip at a first predetermined distance from an outer end of the at least one fiber tip or an end of the fiber tip opposite to an end of the fiber tip that is connected to the optical fiber means that a reference reflection is introduced to define a predetermined or desired measurement section.
- When an optical signal, for example light, propagates in a medium, for example an optical fiber, the light wave undergoes a phase shift, wherein the phase shift can be defined as a function of the temperature. By further introducing a reference reflection, the accuracy of the determination of the temperature can be increased without having to costly amend the apparatus as such.
- Thus, an improved apparatus for determining the temperature of at least one fluid is provided.
- In one embodiment, the apparatus further comprises a second additional reflector that is introduced in the at least one fiber tip at a second predetermined distance from the first additional reflector.
- A second additional reflector being introduced into the at least one fiber tip at a second predetermined distance from the first additional reflectometer means that a further, second reference reflection is introduced to define a further predefined or desired measurement selection.
- In particular, as the phase change between the first light signal and the second light signal is usually periodic over the temperature, this second additional reflector can, for example, be used as additional reflection with shorter distance to increase a range of unique measurements.
- Therein, the coherent detector can be configured to determine the temperature of the at least one fluid by receiving the first light signal, the second light signal and a third light signal that corresponds to parts of the optical signal that are reflected at the second additional reflector when the at least one fiber tip is inserted into the fluid, by determining a difference of the optical phases of the first light signal and the second light signal and a difference of the optical phases of the second light signal and the third light signal, and by determining the temperature of the at least one fluid based on the difference of the optical phases of the first light signal and the second light signal and the difference of the optical phases of the second light signal and the third light signal.
- Therein, the temperature of the at least one fluid being determined based on the difference of the optical phases of the first light signal and the second light signal and the difference of the optical phases of the second light signal and the third light signal can for example mean that first, an approximate value of the temperature can be determined based on the difference of the optical phases of the first light signal and the second light signal, wherein, thereafter, a more exact value of the temperature can be determined based on the difference of the optical phases of the second light signal and the third light signal, and wherein the first predetermined distance is greater than the first predetermined distance. Thus, the determining range can be optimized by simultaneously using two different measurement sections, wherein the determining range can be increased based on a first phase change, and wherein, at the same time, also the accuracy of the determined temperature can be further improved based on a second phase change.
- In a further embodiment, the apparatus comprises a plurality of fiber tips, wherein the apparatus is configured in such a way that all fiber tips can simultaneously be inserted into a plurality of fluids in such a way that each of the plurality of fiber tips is inserted in a different one of the plurality of fluids, and wherein the processing apparatus is configured to determine the temperature of each of the plurality of fluids. Thus, multiple fiber tips can be used in parallel, thereby increasing the capacities of a corresponding test, wherein the signals of the plurality of fiber tips can be multiplexed, for example time multiplexed via delay lines, and wherein the signal processor can be programmed in such a way that it respectively knows when the signals of each of the plurality of fiber tips are processed.
- Further, the processing apparatus can be a coherent optical time domain reflectometer.
- An optical domain reflectometer (OTDR) periodically transmits a detection pulse to an optical fiber. The detection pulse will have Fresnel reflection at certain points in the fiber, where the refractive index changes or imperfections are introduced in the fiber. The Fresnel reflection signal from the optical signal is then received and analyzed. A coherent optical time domain reflectometer (COTDR) further works by means of coherent detection technology.
- Compared for example to optical time domain reflectometers, coherent optical time domain reflectometers have an improved sensitivity and are suitable to detect and determine a phase change, for example the difference of the optical phases of the first light signal and the second light signal.
- According to a further embodiment of the invention, a system for determining the temperature of at least one fluid is provided, wherein the system comprises at least one fluid reservoir and an apparatus for determining the temperature of at least one fluid as described above, wherein the apparatus for determining the temperature of at least one fluid is configured to determine the temperature of at least one fluid contained in the at least one fluid reservoir.
- Thus, a system for determining the temperature of at least one fluid is provided that is based on an improved apparatus for determining the temperature of at least one fluid. In particular, the system is based on an apparatus that takes into account that, when an optical signal, for example light, propagates in a medium, for example an optical fiber, the light wave undergoes a phase shift, wherein the phase shift can be defined as a function of the temperature. By further introducing a reference reflection, the accuracy of the determination of the temperature can be increased without having to costly amend the apparatus as such.
- According to yet a further embodiment of the invention, a method for determining the temperature of at least one fluid is provided, wherein the method comprises inserting at least one fiber tip into at least one fluid, wherein the fiber tip is connected to an optical fiber, and wherein a first additional reflector is introduced into the at least one fiber tip at a first predetermined distance from an outer end of the at least one fiber tip, and determining the temperature of the at least one fluid through a processing apparatus that is connected to a second end of the optical fiber by launching an optical signal into the optical fiber, receiving a first light signal that corresponds to parts of the optical signal that are reflected at the outer end of the at least one fiber tip and a second light signal that corresponds to parts of the optical signal that are reflected at the first additional reflector, determining a difference of the optical phases of the first light signal and the second light signal, and determining the temperature of the at least one fluid based on the difference of the optical phases of the first light signal and the second light signal.
- Thus, an improved method for determining the temperature of at least one fluid is provided. In particular, the method takes into account that, when an optical signal, for example light, propagates in a medium, for example an optical fiber, the light wave undergoes a phase shift, wherein the phase shift can be defined as a function of the temperature. By further introducing a reference reflection, the accuracy of the determination of the temperature can be increased without having to costly amend the apparatus as such.
- In one embodiment, a second additional reflector is introduced in the at least one fiber tip at a second predetermined distance from the first additional reflector, and wherein the step of determining the temperature of the at least one fluid through the processing apparatus comprises launching an optical signal into the optical fiber, receiving the first light signal, the second light signal and a third light signal that corresponds to parts of the optical signal that are reflected at the second additional reflector when the at least one fiber tip is inserted into the fluid, determining a difference of the optical phases of the first light signal and the second light signal and a difference of the optical phases of the second light signal and the third light signal, and determining the temperature of the at least one fluid based on the difference of the optical phases of the first light signal and the second light signal and the difference of the optical phases of the second light signal and the third light signal. Thus, the determining range can be optimized by simultaneously using two different measurement sections, wherein the determining range can be increased based on a first phase change, and wherein, at the same time, also the accuracy of the determined temperature can be further improved based on a second phase change.
- In a further embodiment, a plurality of fiber tips are simultaneously inserted into a plurality of fluids in such a way that each of the plurality of fiber tips is inserted in a different one of the plurality of fluids, and wherein the temperature of each of the plurality of fluids is determined by the processing apparatus. Thus, multiple fiber tips can be used in parallel, thereby increasing the capacities of a corresponding test, wherein the signals of the plurality of fiber tips can be multiplexed, for example time multiplexed via delay lines, and wherein the signal processor can be programmed in such a way that it respectively knows when the signals of each of the plurality of fiber tips are processed.
- The processing apparatus includes a coherent detector. Coherent detectors usually have a high sensitivity and are suitable to detect or determine the phase change.
- Therein, the processing apparatus can be a coherent optical time domain reflectometer. Compared to, for example, to optical time domain reflectometers, coherent optical time domain reflectometers have an improved sensitivity and are suitable to detect and determine a phase change, for example the difference of the optical phases of the first light signal and the second light signal.
- Embodiments of the invention will now be described with reference to the drawings.
- Fig. 1
- illustrates an apparatus for determining the temperature of at least one fluid according to embodiments of the invention;
- Fig. 2
- illustrates a flowchart of a method for determining the temperature of at least one fluid according to a first embodiment;
-
Fig. 1 illustrates an apparatus for determining the temperature of at least onefluid 1 according to embodiments of the invention. - During chemical or biological tests, for example a PCR (polymerase chain reaction) test, the temperature of a small amount of fluid needs to be measured. For example, during chemical or biological tests, it can be required to monitor the temperature change which accompanies a reaction. Therein, the accuracy and precision of the apparatus that is used to monitor the temperature are one of the main factors which affect such measurement.
- During such chemical or biological tests, a small amount of fluid, for example 100µl, is usually inserted into small wells, wherein a plurality of wells can be contained in a common plate, and wherein each well usually has a depth of approximately only 20mm.
- According to the embodiments of
Fig. 1 , an apparatus for determining the temperature of at least onefluid 1 is provided, wherein theapparatus 1 comprises anoptical fiber 2, wherein a first end of theoptical fiber 3 is connected to at least onefiber tip 4, and a firstadditional reflector 5 introduced into the at least onefiber tip 3 at a first predetermined distance from an outer end of the at least onefiber tip 6, and wherein a second end of theoptical fiber 7, opposite the first end of thefiber tip 3, is connected to aprocessing apparatus 8, wherein theprocessing apparatus 8 comprises anoptical source 9, wherein theoptical source 9 is configured to launch an optical signal into theoptical fiber 2, and acoherent detector 10, wherein thecoherent detector 10 is configured to determine the temperature of at least one fluid and comprises areceiving device 11 that is configured to receive a first light signal that corresponds to parts of the optical signal that are reflected at the outer end of the at least onefiber tip 6 when the at least onefiber tip 4 is inserted into the at least one fluid and a second light signal that corresponds to parts of the optical signal that are reflected at the firstadditional reflector 5 when the at least onefiber tip 4 is inserted into the at least one fluid, a first determiningdevice 12 that is configured to determine a difference of the optical phases of the first light signal and the second light signal, and a second determiningdevice 13 that is configured to determine the temperature of the at least one fluid based on the difference of the optical phases of the first light signal and the second light signal. - When an optical signal, for example light, propagates in a medium, for example an optical fiber, the light wave undergoes a phase shift, wherein the phase shift can be defined as a function of the temperature. By further introducing a reference reflection, the accuracy of the determination of the temperature can be increased without having to costly amend the apparatus as such.
- Thus, an improved apparatus for determining the temperature of at least one
fluid 1 is provided. - Therein, the receiving device can for example be realized by a signal receiver. The first determining device and the second determining device can, for example, further be realized by code, that is stored in a memory and executable by a processor.
- According to the embodiments of
Fig. 1 , a bare fiber tip, which has a length of approximately 10mm, is used to probe the temperature of the fluid, which changes the refractive index of the corresponding fiber glass. Therein, the at least one fiber tip has been produced by cleaving the tip of theoptical fiber 2 and removing the coating of the optical fiber over a section of about 10mm. - Further, the first predetermined distance is approximately 10mm, wherein the first
additional reflector 5 has been introduced close to a transition between the at least onefiber tip 4 and theoptical fiber 2, or close to the first end of theoptical fiber 3, respectively, wherein the first additional reflector can for example be introduced by splicing, UV inscription, scrimping, e-beam writing, or any other known suitable method. - Therein, the corresponding phase change in the at least one
fiber tip 4 is used as a measure for the temperature change based on the first additional reflector as measurement section. - Further, the optical source is configured in such a way that reflected signals from the fiber tip can be distinguished from additional reflection.
- As shown in
Fig. 1 , theapparatus 1 further comprises a secondadditional reflector 14 that is introduced in the at least onefiber tip 4 at a predetermined distance from the first additional reflector. - For standard optical fibers the phase change over 10mm and a temperature change of 8K is approximately 2π and therefore, periodic over the temperature, wherefore in this case, the temperature determination is not unique anymore. For this purpose, a second smaller reflection is introduced into the
fiber tip 4, wherein, according to the embodiments ofFig. 1 , this secondadditional reflector 14 is introduced into thefiber tip 4 approximately 1mm after the firstadditional reflector 5, and wherein the second additional reflector can again, for example, be introduced by splicing, UV inscription, scrimping, e-beam writing, or any other known suitable method. - Therein, the second
additional reflector 14 is used to increase the range of unique measurements. According to the embodiments ofFig. 1 , theprocessing apparatus 8 is configured to determine the temperature of the at least one fluid by launching an optical signal into the optical fiber, wherein the receivingdevice 11 is configured to receive the first light signal, the second light signal and a third light signal that corresponds to parts of the optical signal that are reflected at the secondadditional reflector 14 when the at least onefiber tip 4 is inserted into the fluid, wherein the first determiningdevice 12 is configured to determine a difference of the optical phases of the first light signal and the second light signal and a difference of the optical phases of the second light signal and the third light signal, and wherein the second determiningdevice 13 is configured to determine the temperature of the at least one fluid based on the difference of the optical phases of the first light signal and the second light signal and the difference of the optical phases of the second light signal and the third light signal. - Further, although only one fiber tip is shown in
Fig. 1 , the apparatus can also comprise a plurality of fiber tips, wherein the apparatus can be configured in such a way that all fiber tips can simultaneously be inserted into a plurality of fluids in such a way that each of the plurality of fiber tips is inserted in a different one of the plurality of fluids, and wherein the processing apparatus is configured to determine the temperature of each of the plurality of fluids. For example, this can be achieved by using a 1xN fiber coupler and lead fibers and/or time-multiplexing the signals from the individual fiber tips via delay lines. - According to the embodiments of
Fig. 1 , theprocessing apparatus 8 is further a coherent opticaltime domain reflectometer 15. - Using the apparatus shown in
Fig. 1 , the temperature of a small fluid amount of for example 100µl can be determined with asmall fiber tip 4 without interfering with the temperature of the fluid, wherein a high measurement or determination speed, respectively, is enabled. -
Fig. 2 illustrates a flowchart of a method for determining the temperature of at least one fluid 20 according to embodiments of the invention. - As shown in
Fig. 2 , themethod 20 comprises astep 21 of inserting at least one fiber tip into at least one fluid, wherein the fiber tip is connected to an optical fiber, and wherein an additional first reflector is introduced into the at least one fiber tip at a first predetermined distance from an outer end of the at least one fiber tip, and astep 22 of determining the temperature of the at least one fluid, wherein thestep 22 of determining the temperature of the at least one fluid comprises astep 23 of launching an optical signal into the optical fiber, astep 24 of receiving a first light signal that corresponds to parts of the optical signal that are reflected at the outer end of the at least one fiber tip and a second light signal that corresponds to parts of the optical signal that are reflected at the first additional reflector, astep 25 of determining a difference of the optical phases of the first light signal and the second light signal, and astep 26 of determining the temperature of the at least one fluid based on the difference of the optical phases of the first light signal and the second light signal. - According to the embodiments of
Fig. 2 , a second additional reflector is introduced into the at least one fiber tip at a second predetermined distance from the first additional reflectometer, wherein instep 24, the first light signal, the second light signal and a third light signal that corresponds to parts of the optical signal that are reflected at the second additional reflector when the at least one fiber tip is inserted into the fluid are received, wherein instep 25, a difference of the optical phases of the first light signal and the second light signal and a difference of the optical phases of the second light signal and the third light signal is determined, and wherein instep 26, the temperature of the at least one fluid is determined based on the difference of the optical phases of the first light signal and the second light signal and the difference of the optical phases of the second light signal and the third light signal. - Further, according to the embodiments of
Fig. 2 , a plurality of fiber tips are simultaneously inserted into a plurality of fluids instep 21 in such a way that each of the plurality of fiber tips is inserted in a different one of the plurality of fluids, and wherein themethod 20 further comprises determining the temperature of each of the plurality of fluids, or whereinstep 23 of determining the temperature of the at least one fluid is separately executed for each of the wells, respectively. - Therein, the method for determining the temperature of at least one fluid 20 shown in
Fig. 2 can for example be used to monitor or determine the temperature during a biological or chemical test. -
- 1
- apparatus
- 2
- optical fiber
- 3
- first end
- 4
- fiber tip
- 5
- first additional reflector
- 6
- outer end
- 7
- second end
- 8
- processing apparatus
- 9
- optical source
- 10
- signal processor
- 11
- receiving device
- 12
- first determining device
- 13
- second determining device
- 14
- second additional reflector
- 15
- coherent optical time reflectometer
- 20
- method
- 21
- step
- 22
- step
- 23
- step
- 24
- step
- 25
- step
- 26
- step
Claims (10)
- Apparatus for determining the temperature of at least one fluid, wherein the apparatus (1) comprises an optical fiber (2), wherein a first end of the optical fiber (3) is connected to at least one fiber tip (4), and a first additional reflector (5) introduced into the at least one fiber tip (4) at a first predetermined distance from an outer end of the at least one fiber tip (6), and wherein a second end of the optical fiber (7) is connected to a processing apparatus (8), wherein the processing apparatus (8) comprises an optical source (9), wherein the optical source (9) is configured to launch an optical signal into the optical fiber, and a coherent detector (10), wherein the coherent detector (10) is configured to determine the temperature of at least one fluid by receiving a first light signal that corresponds to parts of the optical signal that are reflected at the outer end of the at least one fiber tip (6) when the at least one fiber tip (4) is inserted into the at least one fluid and a second light signal that corresponds to parts of the optical signal that are reflected at the first additional reflector (5) when the at least one fiber tip (4) is inserted into the at least one fluid, determining a difference of the optical phases of the first light signal and the second light signal, and determining the temperature of the at least one fluid based on the difference of the optical phases of the first light signal and the second light signal.
- Apparatus according to claim 1, wherein the apparatus further comprises a second additional reflector (14) that is introduced into the at least one fiber tip at a second predetermined distance from the first additional reflector.
- Apparatus according to claim 2, wherein the coherent detector (10) is configured to determine the temperature of the at least one fluid by receiving the first light signal, the second light signal and a third light signal that corresponds to parts of the optical signal that are reflected at the second additional reflector (14) when the at least one fiber tip (4) is inserted into the fluid, by determining a difference of the optical phases of the first light signal and the second light signal and a difference of the optical phases of the second light signal and the third light signal, and by determining the temperature of the at least one fluid based on the difference of the optical phases of the first light signal and the second light signal and the difference of the optical phases of the second light signal and the third light signal.
- Apparatus according to one of claims 1 to 3, wherein the apparatus comprises a plurality of fiber tips, wherein the apparatus is configured in such a way that all fiber tips can simultaneously be inserted into a plurality of fluids in such a way that each of the plurality of fiber tips is inserted in a different one of the plurality of fluids, and wherein the signal processor is configured to determine the temperature of each of the plurality of fluids.
- Apparatus according to one of claims 1 to 4, wherein the processing apparatus is a coherent optical time domain reflectometer (15).
- System for determining the temperature of at least one fluid, wherein the system comprises at least one fluid reservoir and an apparatus for determining the temperature of at least one fluid according to one of claims 1 to 5, wherein the apparatus for determining the temperature of at least one fluid is configured to determine the temperature of at least one fluid contained in the at least one fluid reservoir.
- Method for determining the temperature of at least one fluid, wherein the method (20) comprises the steps of:- inserting at least one fiber tip into at least one fluid, wherein the at least one fiber tip is connected to an optical fiber, and wherein an additional first reflector is introduced into the at least one fiber tip at a first predetermined distance from an outer end of the at least one fiber tip (21); and- determining the temperature of the at least one fluid through a processing apparatus connected to a second end of the optical signal (22), wherein the determining the temperature of the at least one fluid (22) comprises launching an optical signal into the optical fiber (23), receiving a first light signal that corresponds to parts of the optical signal that are reflected at the outer end of the at least one fiber tip and a second light signal that corresponds to parts of the optical signal that are reflected at the first additional reflector (24), determining a difference of the optical phases of the first light signal and the second light signal (25), and determining the temperature of the at least one fluid based on the difference of the optical phases of the first light signal and the second light signal (26).
- Method according to claim 7, wherein a second additional reflector is introduced into the at least one fiber tip at a second predetermined distance from the first additional reflector, and wherein the step of determining the temperature of the at least one fluid comprises (22) the steps of:- launching an optical signal into the optical fiber (23);- receiving the first light signal, the second light signal and a third light signal that corresponds to parts of the optical signal that are reflected at the second additional reflector when the at least one fiber tip is inserted into the fluid (24);- determining a difference of the optical phases of the first light signal and the second light signal and a difference of the optical phases of the second light signal and the third light signal (25); and- determining the temperature of the at least one fluid based on the difference of the optical phases of the first light signal and the second light signal and the difference of the optical phases of the second light signal and the third light signal (26).
- Method according to claim 7 or 8, wherein a plurality of fiber tips are simultaneously inserted into a plurality of fluids in such a way that each of the plurality of fiber tips is inserted in a different one of the plurality of fluids, and wherein the temperature of each of the plurality of fluids is determined by the processing apparatus.
- Method according to one of claims 7 to 9, wherein the coherent detector is a coherent optical time domain reflectometer.
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EP22151428.4A EP4212841A1 (en) | 2022-01-13 | 2022-01-13 | Apparatus for determining the temperature of at least one fluid |
US18/096,290 US20230221190A1 (en) | 2022-01-13 | 2023-01-12 | Apparatus for Determining the Temperature of At Least One Fluid |
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EP22151428.4A EP4212841A1 (en) | 2022-01-13 | 2022-01-13 | Apparatus for determining the temperature of at least one fluid |
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Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5231681A (en) | 1990-12-14 | 1993-07-27 | Telefonaktiebolaget Lm Ericsson | Optical fibre cable for detecting a change in temperature |
US20070006663A1 (en) * | 2003-09-04 | 2007-01-11 | Zerwekh Paul S | Optical sensor with co-located pressure and temperature sensors |
US20180223647A1 (en) * | 2017-02-03 | 2018-08-09 | Baker Hughes Incorporated | Measurement of temperature using combination of rayleigh and raman backscatter interferometry |
US20210052173A1 (en) * | 2018-01-22 | 2021-02-25 | Ucl Business Ltd | A sensor for measuring a flow of a fluid |
-
2022
- 2022-01-13 EP EP22151428.4A patent/EP4212841A1/en active Pending
-
2023
- 2023-01-12 US US18/096,290 patent/US20230221190A1/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5231681A (en) | 1990-12-14 | 1993-07-27 | Telefonaktiebolaget Lm Ericsson | Optical fibre cable for detecting a change in temperature |
US20070006663A1 (en) * | 2003-09-04 | 2007-01-11 | Zerwekh Paul S | Optical sensor with co-located pressure and temperature sensors |
US20180223647A1 (en) * | 2017-02-03 | 2018-08-09 | Baker Hughes Incorporated | Measurement of temperature using combination of rayleigh and raman backscatter interferometry |
US20210052173A1 (en) * | 2018-01-22 | 2021-02-25 | Ucl Business Ltd | A sensor for measuring a flow of a fluid |
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