EP4196603A1 - Procédés se rapportant à la synthèse enzymatique de novo d'acides nucléiques - Google Patents
Procédés se rapportant à la synthèse enzymatique de novo d'acides nucléiquesInfo
- Publication number
- EP4196603A1 EP4196603A1 EP21755815.4A EP21755815A EP4196603A1 EP 4196603 A1 EP4196603 A1 EP 4196603A1 EP 21755815 A EP21755815 A EP 21755815A EP 4196603 A1 EP4196603 A1 EP 4196603A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- capping
- nucleotide
- strands
- nucleic acid
- species
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
L'invention concerne des procédés et des compositions pour assurer la qualité de la synthèse d'oligonucléotides pendant des cycles d'extension de transférase terminale indépendante de la molécule matrice. Plus particulièrement, l'invention concerne des procédés et des kits contenant un procédé pour coiffer et retirer les brins non allongés qui n'ont pas réussi à incorporer correctement des nucléotides terminés.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB2012544.9A GB202012544D0 (en) | 2020-08-12 | 2020-08-12 | Methods relating to de novo enzymatic mucleic acid synthesis |
| PCT/GB2021/052095 WO2022034331A1 (fr) | 2020-08-12 | 2021-08-12 | Procédés se rapportant à la synthèse enzymatique de novo d'acides nucléiques |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EP4196603A1 true EP4196603A1 (fr) | 2023-06-21 |
Family
ID=72520099
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP21755815.4A Pending EP4196603A1 (fr) | 2020-08-12 | 2021-08-12 | Procédés se rapportant à la synthèse enzymatique de novo d'acides nucléiques |
Country Status (3)
| Country | Link |
|---|---|
| EP (1) | EP4196603A1 (fr) |
| GB (1) | GB202012544D0 (fr) |
| WO (1) | WO2022034331A1 (fr) |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10683536B2 (en) * | 2013-04-02 | 2020-06-16 | Molecular Assemblies, Inc. | Reusable initiators for synthesizing nucleic acids |
| WO2019051250A1 (fr) | 2017-09-08 | 2019-03-14 | Sigma-Aldrich Co. Llc | Synthèse de polynucléotides dépendant d'une matrice et à médiation par polymérase |
| GB201714827D0 (en) * | 2017-09-14 | 2017-11-01 | Nuclera Nucleics Ltd | Novel use |
| CN111542532B (zh) | 2017-10-04 | 2024-03-15 | 生捷科技控股公司 | 酶法合成寡核苷酸的方法和体系 |
-
2020
- 2020-08-12 GB GBGB2012544.9A patent/GB202012544D0/en not_active Ceased
-
2021
- 2021-08-12 WO PCT/GB2021/052095 patent/WO2022034331A1/fr unknown
- 2021-08-12 EP EP21755815.4A patent/EP4196603A1/fr active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| WO2022034331A1 (fr) | 2022-02-17 |
| GB202012544D0 (en) | 2020-09-23 |
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