EP4132674A4 - Devices and methods for sequencing - Google Patents

Devices and methods for sequencing Download PDF

Info

Publication number
EP4132674A4
EP4132674A4 EP21792373.9A EP21792373A EP4132674A4 EP 4132674 A4 EP4132674 A4 EP 4132674A4 EP 21792373 A EP21792373 A EP 21792373A EP 4132674 A4 EP4132674 A4 EP 4132674A4
Authority
EP
European Patent Office
Prior art keywords
sequencing
methods
devices
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP21792373.9A
Other languages
German (de)
French (fr)
Other versions
EP4132674A1 (en
Inventor
Jonathan M. Rothberg
John H. Leamon
Jonathan C. Schultz
Michele Millham
Caixia Lv
Haidong Huang
Roger Nani
Omer AD
Brian Reed
Matthew Dyer
Robert E. BOER
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Quantum Si Inc
Original Assignee
Quantum Si Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Quantum Si Inc filed Critical Quantum Si Inc
Publication of EP4132674A1 publication Critical patent/EP4132674A1/en
Publication of EP4132674A4 publication Critical patent/EP4132674A4/en
Pending legal-status Critical Current

Links

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D17/00Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
    • B01D17/06Separation of liquids from each other by electricity
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818Sequencing of polypeptides
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16Reagents, handling or storing thereof
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0475Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122Massive parallel sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/159Reduction of complexity, e.g. amplification of subsets, removing duplicated genomic regions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60Detection means characterised by use of a special device
    • C12Q2565/629Detection means characterised by use of a special device being a microfluidic device

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Thermal Sciences (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
EP21792373.9A 2020-04-22 2021-04-21 Devices and methods for sequencing Pending EP4132674A4 (en)

Applications Claiming Priority (10)

Application Number Priority Date Filing Date Title
US202063014093P 2020-04-22 2020-04-22
US202063014071P 2020-04-22 2020-04-22
US202063014106P 2020-04-22 2020-04-22
US202063014087P 2020-04-22 2020-04-22
US202063041206P 2020-06-19 2020-06-19
US202163139343P 2021-01-20 2021-01-20
US202163139348P 2021-01-20 2021-01-20
US202163139339P 2021-01-20 2021-01-20
US202163139346P 2021-01-20 2021-01-20
PCT/US2021/028471 WO2021216763A1 (en) 2020-04-22 2021-04-21 Devices and methods for sequencing

Publications (2)

Publication Number Publication Date
EP4132674A1 EP4132674A1 (en) 2023-02-15
EP4132674A4 true EP4132674A4 (en) 2024-05-29

Family

ID=78269941

Family Applications (1)

Application Number Title Priority Date Filing Date
EP21792373.9A Pending EP4132674A4 (en) 2020-04-22 2021-04-21 Devices and methods for sequencing

Country Status (7)

Country Link
EP (1) EP4132674A4 (en)
JP (1) JP2023523592A (en)
KR (1) KR20220159496A (en)
CN (1) CN115916365A (en)
AU (1) AU2021261356A1 (en)
CA (1) CA3177368A1 (en)
WO (1) WO2021216763A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2018353924A1 (en) 2017-12-29 2019-07-18 Clear Labs, Inc. Automated priming and library loading device

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020022261A1 (en) * 1995-06-29 2002-02-21 Anderson Rolfe C. Miniaturized genetic analysis systems and methods
US20100273142A1 (en) * 2007-12-20 2010-10-28 Koninklijke Philips Electronics N.V. Multi-compartment device with magnetic particles
WO2016065300A1 (en) * 2014-10-24 2016-04-28 Eshoo Mark W Microfluidic cartridge
US20190212233A1 (en) * 2016-11-29 2019-07-11 Stevan Jovanovich Method and apparatus for processing tissue samples

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6818185B1 (en) * 1999-05-28 2004-11-16 Cepheid Cartridge for conducting a chemical reaction
US7541166B2 (en) * 2003-09-19 2009-06-02 Microfluidic Systems, Inc. Sonication to selectively lyse different cell types
CN101990516B (en) * 2008-01-22 2015-09-09 英特基因有限公司 Multiplex sample preparation system and the use in integrated analysis system thereof
US9156010B2 (en) * 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US9506934B2 (en) * 2013-04-29 2016-11-29 Honeywell International Inc. Polymer test cartridge mixer for cell lysis

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020022261A1 (en) * 1995-06-29 2002-02-21 Anderson Rolfe C. Miniaturized genetic analysis systems and methods
US20100273142A1 (en) * 2007-12-20 2010-10-28 Koninklijke Philips Electronics N.V. Multi-compartment device with magnetic particles
WO2016065300A1 (en) * 2014-10-24 2016-04-28 Eshoo Mark W Microfluidic cartridge
US20190212233A1 (en) * 2016-11-29 2019-07-11 Stevan Jovanovich Method and apparatus for processing tissue samples

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
AYSE NUR POLAT ET AL: "Fully automated isotopic dimethyl labeling and phosphopeptide enrichment using a microfluidic HPLC phosphochip", ANALYTICAL AND BIOANALYTICAL CHEMISTRY, SPRINGER, BERLIN, DE, vol. 404, no. 8, 14 September 2012 (2012-09-14), pages 2507 - 2512, XP035127370, ISSN: 1618-2650, DOI: 10.1007/S00216-012-6395-7 *
See also references of WO2021216763A1 *

Also Published As

Publication number Publication date
KR20220159496A (en) 2022-12-02
WO2021216763A1 (en) 2021-10-28
JP2023523592A (en) 2023-06-06
CA3177368A1 (en) 2021-10-28
CN115916365A (en) 2023-04-04
EP4132674A1 (en) 2023-02-15
AU2021261356A1 (en) 2022-12-15

Similar Documents

Publication Publication Date Title
EP4054258A4 (en) Sidelink positioning method and apparatus
EP3968133A4 (en) Air-mouse mode implementation method and related device
EP3910364A4 (en) Positioning method and device
EP4030834A4 (en) Bluetooth connection method and related apparatus
EP4024918A4 (en) Bluetooth connection method and related apparatus
EP4016457A4 (en) Positioning method and apparatus
EP3812707A4 (en) Positioning method and positioning device
EP4030809A4 (en) Configuration method and apparatus
EP4074183A4 (en) Concentration method and equipment
EP4084397A4 (en) Synchronization device and synchronization method
EP4044466A4 (en) Synchronization method and device
EP4115995A4 (en) Sorting-conveying apparatus and sorting-conveying system
EP4030719A4 (en) Configuration method and related device
EP3933975A4 (en) Electrode pre-lithiation method and device
EP3972331A4 (en) Positioning method and device
EP3987219A4 (en) Light-emitting apparatus using metasurfaces and light-emitting method thereof
EP4148408A4 (en) Automotive-body-adhesion-position optimization-analysis method and device
EP4080914A4 (en) Configuration enabling method and apparatus
EP3943124A4 (en) Gas treatment device and gas treatment method
EP4079397A4 (en) Synthesis device and synthesis method
EP4132674A4 (en) Devices and methods for sequencing
EP4246953A4 (en) Configuration method and apparatus
EP4171098A4 (en) Configuration method and apparatus
EP4113424A4 (en) Construction-model-creating device and construction-model-creating method
EP3968358A4 (en) Gas treatment method and gas treatment device

Legal Events

Date Code Title Description
STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE

PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE

17P Request for examination filed

Effective date: 20221107

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

DAV Request for validation of the european patent (deleted)
DAX Request for extension of the european patent (deleted)
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 40087778

Country of ref document: HK

A4 Supplementary search report drawn up and despatched

Effective date: 20240426

RIC1 Information provided on ipc code assigned before grant

Ipc: B01J 19/08 20060101ALI20240422BHEP

Ipc: B01D 57/02 20060101ALI20240422BHEP

Ipc: B01D 17/06 20060101ALI20240422BHEP

Ipc: B01D 17/00 20060101AFI20240422BHEP