EP4090754A4 - Tunable transposon systems - Google Patents

Tunable transposon systems Download PDF

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Publication number
EP4090754A4
EP4090754A4 EP21741482.0A EP21741482A EP4090754A4 EP 4090754 A4 EP4090754 A4 EP 4090754A4 EP 21741482 A EP21741482 A EP 21741482A EP 4090754 A4 EP4090754 A4 EP 4090754A4
Authority
EP
European Patent Office
Prior art keywords
tunable
transposon systems
transposon
systems
tunable transposon
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP21741482.0A
Other languages
German (de)
French (fr)
Other versions
EP4090754A2 (en
Inventor
Kathy NGO
Jenifer WOO
Binh Pham
Vu Truong-Le
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Aridis Pharmaceuticals Inc
Original Assignee
Aridis Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aridis Pharmaceuticals Inc filed Critical Aridis Pharmaceuticals Inc
Publication of EP4090754A2 publication Critical patent/EP4090754A2/en
Publication of EP4090754A4 publication Critical patent/EP4090754A4/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C12N2830/003—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor tet inducible

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
EP21741482.0A 2020-01-18 2021-01-17 Tunable transposon systems Pending EP4090754A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202062963022P 2020-01-18 2020-01-18
PCT/US2021/013788 WO2021146666A2 (en) 2020-01-18 2021-01-17 Tunable transposon systems

Publications (2)

Publication Number Publication Date
EP4090754A2 EP4090754A2 (en) 2022-11-23
EP4090754A4 true EP4090754A4 (en) 2024-04-24

Family

ID=76864287

Family Applications (1)

Application Number Title Priority Date Filing Date
EP21741482.0A Pending EP4090754A4 (en) 2020-01-18 2021-01-17 Tunable transposon systems

Country Status (4)

Country Link
EP (1) EP4090754A4 (en)
CN (1) CN115279910A (en)
CA (1) CA3161872A1 (en)
WO (1) WO2021146666A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023060589A1 (en) * 2021-10-15 2023-04-20 深圳市深研生物科技有限公司 Multi-transposon system
CN114657153A (en) * 2022-05-24 2022-06-24 上海健士拜生物科技有限公司 rAAV recombinant packaging plasmid, plasmid system for rAAV packaging and preparation method of rAAV

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015188191A1 (en) * 2014-06-06 2015-12-10 Wong Wilson W Dna recombinase circuits for logical control of gene expression

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110023143A1 (en) * 2008-12-04 2011-01-27 Sigma-Aldrich Co. Genomic editing of neurodevelopmental genes in animals
CN105154473B (en) * 2015-09-30 2019-03-01 上海细胞治疗研究院 A kind of transposon integration system of highly effective and safe and application thereof
EP3367788A4 (en) * 2015-10-27 2019-07-31 Recombinetics, Inc. Engineering of humanized car t-cells and platelets by genetic complementation
US20200054711A1 (en) * 2017-02-28 2020-02-20 The Penn State Research Foundation Regenerating functional neurons for treatment of neural injury caused by disruption of blood flow

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015188191A1 (en) * 2014-06-06 2015-12-10 Wong Wilson W Dna recombinase circuits for logical control of gene expression

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
BRIAN E HEW ET AL: "RNA-guided piggyBac transposition in human cells", SYNTHETIC BIOLOGY, vol. 4, no. 1, 2 July 2019 (2019-07-02), pages ysz018, XP055737951, DOI: 10.1093/synbio/ysz018 *
DUPUY ADAM J. ET AL: "A Modified Sleeping Beauty Transposon System That Can Be Used to Model a Wide Variety of Human Cancers in Mice", CANCER RESEARCH, vol. 69, no. 20, 14 October 2009 (2009-10-14), US, pages 8150 - 8156, XP093140527, ISSN: 0008-5472, Retrieved from the Internet <URL:https://aacrjournals.org/cancerres/article-pdf/69/20/8150/2615753/8150.pdf> DOI: 10.1158/0008-5472.CAN-09-1135 *
ERIC KOWARZ ET AL: "Optimized Sleeping Beauty transposons rapidly generate stable transgenic cell lines", BIOTECHNOLOGY JOURNAL, vol. 10, no. 4, 2 March 2015 (2015-03-02), DE, pages 647 - 653, XP055684081, ISSN: 1860-6768, DOI: 10.1002/biot.201400821 *
FEDOROVA SVETLANA A ET AL: "Protein trap: a new Swiss army knife for geneticists?", MOLECULAR BIOLOGY REPORTS, SPRINGER NETHERLANDS, NL, vol. 47, no. 2, 14 November 2019 (2019-11-14), pages 1445 - 1458, XP036987601, ISSN: 0301-4851, [retrieved on 20191114], DOI: 10.1007/S11033-019-05181-Z *
MARTIN HOLZENBERGER ET AL: "Ubiquitous postnatal LoxP recombination using a doxycycline auto-inducible Cre transgene (DAI-Cre)", GENESIS, WILEY-LISS, HOBOKEN, USA, vol. 26, no. 2, 23 February 2000 (2000-02-23), pages 157 - 159, XP072302733, ISSN: 1526-954X, DOI: 10.1002/(SICI)1526-968X(200002)26:2<157::AID-GENE19>3.0.CO;2-R *
MATTIA MATASCI ET AL: "The PiggyBac transposon enhances the frequency of CHO stable cell line generation and yields recombinant lines with superior productivity and stability", BIOTECHNOLOGY AND BIOENGINEERING, JOHN WILEY, HOBOKEN, USA, vol. 108, no. 9, 25 April 2011 (2011-04-25), pages 2141 - 2150, XP071108998, ISSN: 0006-3592, DOI: 10.1002/BIT.23167 *
NARAYANAVARI SUNEEL A. ET AL: "Sleeping Beauty transposition: from biology to applications", CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 52, no. 1, 4 October 2016 (2016-10-04), US, pages 18 - 44, XP055864384, ISSN: 1040-9238, Retrieved from the Internet <URL:https://tandfonline.com/doi/pdf/10.1080/10409238.2016.1237935> DOI: 10.1080/10409238.2016.1237935 *

Also Published As

Publication number Publication date
WO2021146666A8 (en) 2022-08-11
WO2021146666A3 (en) 2021-08-26
CN115279910A (en) 2022-11-01
WO2021146666A2 (en) 2021-07-22
CA3161872A1 (en) 2021-07-22
EP4090754A2 (en) 2022-11-23

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