EP4028817A4 - Cytométrie de flux à électrophorèse sur gel à une seule cellule - Google Patents

Cytométrie de flux à électrophorèse sur gel à une seule cellule Download PDF

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Publication number
EP4028817A4
EP4028817A4 EP21876723.4A EP21876723A EP4028817A4 EP 4028817 A4 EP4028817 A4 EP 4028817A4 EP 21876723 A EP21876723 A EP 21876723A EP 4028817 A4 EP4028817 A4 EP 4028817A4
Authority
EP
European Patent Office
Prior art keywords
flow cytometry
gel electrophoresis
single cell
cell gel
electrophoresis flow
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP21876723.4A
Other languages
German (de)
English (en)
Other versions
EP4028817A1 (fr
Inventor
Utku Güner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
T C Trakya Ueniversitesi
Original Assignee
T C Trakya Ueniversitesi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by T C Trakya Ueniversitesi filed Critical T C Trakya Ueniversitesi
Publication of EP4028817A1 publication Critical patent/EP4028817A1/fr
Publication of EP4028817A4 publication Critical patent/EP4028817A4/fr
Pending legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0241—Drop counters; Drop formers
    • B01L3/0268—Drop counters; Drop formers using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44786—Apparatus specially adapted therefor of the magneto-electrophoresis type
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
    • G01N35/1016—Control of the volume dispensed or introduced
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1081—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices characterised by the means for relatively moving the transfer device and the containers in an horizontal plane
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0636—Focussing flows, e.g. to laminate flows
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/143—Quality control, feedback systems
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/069—Absorbents; Gels to retain a fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G01N2001/282—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on with mapping; Identification of areas; Spatial correlated pattern
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1034—Transferring microquantities of liquid
    • G01N2035/1041—Ink-jet like dispensers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Electrochemistry (AREA)
  • Optics & Photonics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP21876723.4A 2020-11-24 2021-11-10 Cytométrie de flux à électrophorèse sur gel à une seule cellule Pending EP4028817A4 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
TR2020/18906A TR202018906A2 (tr) 2020-11-24 2020-11-24 Tek hücre jel elektroforezi̇ akiş si̇tometri̇
PCT/TR2021/051177 WO2022115076A1 (fr) 2020-11-24 2021-11-10 Cytométrie de flux à électrophorèse sur gel à une seule cellule

Publications (2)

Publication Number Publication Date
EP4028817A1 EP4028817A1 (fr) 2022-07-20
EP4028817A4 true EP4028817A4 (fr) 2023-01-04

Family

ID=81754769

Family Applications (1)

Application Number Title Priority Date Filing Date
EP21876723.4A Pending EP4028817A4 (fr) 2020-11-24 2021-11-10 Cytométrie de flux à électrophorèse sur gel à une seule cellule

Country Status (4)

Country Link
US (1) US20230415143A1 (fr)
EP (1) EP4028817A4 (fr)
TR (1) TR202018906A2 (fr)
WO (1) WO2022115076A1 (fr)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN201662556U (zh) * 2010-03-09 2010-12-01 浙江大学 一种用于单细胞凝胶电泳试验的成套装置
WO2013052318A1 (fr) * 2011-09-25 2013-04-11 Theranos, Inc. Systèmes et procédés pour multi-analyse
WO2019210243A1 (fr) * 2018-04-26 2019-10-31 The Regents Of The University Of California Procédés et dispositifs utilisant une synthèse de voies d'électrophorèse de microgel adressable individuellement pour dosages biologiques

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4572022B2 (ja) * 2000-07-06 2010-10-27 光夫 板倉 平板ゲル電気泳動装置用試料導入装置およびその試料導入方法
WO2015079048A1 (fr) * 2013-11-29 2015-06-04 Ge Healthcare Bio-Sciences Ab Système d'électrophorèse
EP3265796B1 (fr) * 2015-03-06 2022-11-09 4D Lifetec AG Système d'électrophorèse sur gel conçu pour une électrophorèse sur gel à cellule unique

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN201662556U (zh) * 2010-03-09 2010-12-01 浙江大学 一种用于单细胞凝胶电泳试验的成套装置
WO2013052318A1 (fr) * 2011-09-25 2013-04-11 Theranos, Inc. Systèmes et procédés pour multi-analyse
WO2019210243A1 (fr) * 2018-04-26 2019-10-31 The Regents Of The University Of California Procédés et dispositifs utilisant une synthèse de voies d'électrophorèse de microgel adressable individuellement pour dosages biologiques

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BING-QING SHEN: "Transport and self-assembly of droplets in microfluidic devices", 25 September 2014 (2014-09-25), XP055287627, Retrieved from the Internet <URL:https://tel.archives-ouvertes.fr/tel-01127629/document> [retrieved on 20221122] *
P. SAJEESH ET AL: "Particle separation and sorting in microfluidic devices: a review", MICROFLUIDICS AND NANOFLUIDICS, vol. 17, no. 1, 29 November 2013 (2013-11-29), Berlin/Heidelberg, pages 1 - 52, XP055342704, ISSN: 1613-4982, DOI: 10.1007/s10404-013-1291-9 *
PÉREZ-GARCÍA C ET AL: "Development of a comet-FISH assay for the detection of DNA damage in hemocytes ofCrassostrea gigas", AQUATIC TOXICOLOGY, ELSEVIER, AMSTERDAM, NL, vol. 161, 29 January 2015 (2015-01-29), pages 189 - 195, XP029149323, ISSN: 0166-445X, DOI: 10.1016/J.AQUATOX.2015.01.022 *
See also references of WO2022115076A1 *

Also Published As

Publication number Publication date
EP4028817A1 (fr) 2022-07-20
WO2022115076A1 (fr) 2022-06-02
US20230415143A1 (en) 2023-12-28
TR202018906A2 (tr) 2022-06-21

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