EP3970493B1 - Fertiliser composition including elemental sulfur and trigonelline as an enhancer of microbiological oxidation of elemental sulphur - Google Patents

Fertiliser composition including elemental sulfur and trigonelline as an enhancer of microbiological oxidation of elemental sulphur Download PDF

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Publication number
EP3970493B1
EP3970493B1 EP19935214.7A EP19935214A EP3970493B1 EP 3970493 B1 EP3970493 B1 EP 3970493B1 EP 19935214 A EP19935214 A EP 19935214A EP 3970493 B1 EP3970493 B1 EP 3970493B1
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Prior art keywords
fertiliser
weight
acid
composition according
proportion
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German (de)
English (en)
French (fr)
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EP3970493A4 (en
EP3970493A1 (en
Inventor
Sergio Atares Real
Joaquin Romero Lopez
Ignasi SALAET MADORRAN
Maria FERRER GINES
Tula del Carmen YANCE CHAVEZ
Carlos FUERTES DOÑATE
Marcos CABALLERO MOLADA
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Fertinagro Biotech SL
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Fertinagro Biotech SL
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00—Other inorganic fertilisers
    • C05D9/02—Other inorganic fertilisers containing trace elements
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/02—Sulfur; Selenium; Tellurium; Compounds thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P15/00—Biocides for specific purposes not provided for in groups A01P1/00 - A01P13/00
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/40—Mixtures of one or more fertilisers with additives not having a specially fertilising activity for affecting fertiliser dosage or release rate; for affecting solubility
    • C05G3/44—Mixtures of one or more fertilisers with additives not having a specially fertilising activity for affecting fertiliser dosage or release rate; for affecting solubility for affecting solubility

Definitions

  • the present invention relates to a fertiliser composition including an enhancer of the microbiological oxidation of elemental sulphur (S°), as well as to the use of said fertiliser composition.
  • the invention provides a fertiliser composition that includes trigonelline as an enhancer of the microbiological oxidation of elemental sulphur (S°), wherein trigonelline increases the rate of SO 4 2- generation in the presence of S 0 , the application thereof constituting a source of sulphur for the microbiological release of sulphuric acid in agricultural soils and an alternative to conventional sulphur fertilisers and amendments.
  • S° elemental sulphur
  • the invention relates to a combination of the described fertiliser composition together with an additional fertiliser and/or biostimulant.
  • Sulphur is an essential element for plants as it forms part of the cysteine and methionine amino acids, determining the structural conformation of proteins and the binding thereof to metal ligands ( Ceccotti SP. 1996. Plant nutrient sulphur - A review of nutrient balance, environmental impact and fertilizers. Fertilizer Research 43(1): 117-125 ). In addition, sulphur forms part of numerous vitamins, such as biotin and thiamine, and of essential cofactors, such as in coenzyme A.
  • the SO 4 2- ion although it has a higher adsorption affinity to soil colloids than NO 3 - or Cl - ions, is subject to leaching losses, which contributes to aquifer contamination.
  • sulphur deficiency often occurs naturally due to high mineralisation rates of organic forms ( Ribeiro et al., 2001. Dynamics of Sulfur Fractions in Brazilian Soils Submitted to Consecutive Harvests of Sorghum. Published in Soil Sci. Soc. Am. J. 65:787-794 ).
  • S° elemental sulphur
  • SO 4 2- sulphate
  • microbiological release of sulphuric acid contributes to the solubilisation of certain plant nutrients that are in insoluble forms.
  • An example is the phosphorus present in phosphate rock, the availability of which to plants can be greatly increased by being solubilised by micro-organisms of the soil when applied together with elemental sulphur ( Lipman et al., 1916. Sulfur oxidation in soils and its effect on the availability of mineral phosphates. Soil Science: 2: 499-538 ; Evans et al., 2006. Application of reactive phosphate rock and sulphur fertilisers to enhance the availability of soil phosphate in organic farming. Nutrient Cycling in Agroecosystems 75:233-246 ).
  • the main genera involved in elemental sulphur oxidation in agricultural soils are Acidithiobacillus , Acidianus , Alicyclobacillus, Aquaspirillum, Bacillus, Beggiatoa, Mesorhizobium, Methylobacterium, Parococcus, Pseudomonas, Rhizobium, Starkeya, Thermithiobacillus, Thermothrix, Thiobacillus, Xanthobacter.
  • S° elemental sulphur
  • SO 4 2- sulphate ion
  • patent application WO 2016/126278 describes an enhanced sulphate release fertiliser composition consisting of a mixture of elemental sulphur and humic acids.
  • the present invention is based on the above-mentioned approaches in such a way that, by regulating the sulphur transformation processes in soils, certain organic molecules can, in the presence of elemental sulphur, increase the availability of this nutrient for plants and favour the microbiological release of sulphuric acid in agricultural soils.
  • This approach would also constitute an alternative to conventional sulphur fertilisers and amendments.
  • the present invention provides a fertiliser composition that includes trigonelline as an enhancer of the microbiological oxidation of elemental sulphur to sulphate ion (S 0 to SO 4 2- ) wherein trigonelline increases the levels of SO 4 2- in agricultural soils.
  • Trigonelline or N-methylnicotinic acid, is an alkaloid found in many plants, such as pea, hemp, oats, potato and fenugreek, in which it has various regulatory functions ( Garg, 2016, in Nutraceuticals Efficacy, Safety and Toxicity, pp. 599-617 ). Trigonelline is the methylation product of niacin (vitamin B3).
  • Trigonelline molecule (C 7 H 7 NO 2 )
  • the present invention provides a fertiliser composition that includes elemental sulphur (S°) and trigonelline as an enhancer of the microbiological oxidation of S 0 .
  • the generation of SO 4 2- in trigonelline-treated soil depends on the presence of S 0 and is mediated by soil micro-organisms.
  • the present inventors believe that, in the presence of S 0 , trigonelline stimulates the sulphur-oxidising bacteria present in the soil resulting in increased oxidation of S 0 .
  • the addition of various organic matter stimulates S 0 oxidation; however, the nature of the organic matter has an important influence on this process.
  • the stimulation of S 0 oxidation by the application of trigonelline is greater than that of other organic matter, such as humic acids or glucose, which have a proven effect on S 0 oxidation.
  • trigonelline has a stronger effect on S 0 oxidation than other organic molecules chosen among organic acids, amino acids, alkaloids, sugars and alcohols, which are organic metabolites with the potential to have an inducing effect on soil microorganisms. More specifically, the effect of trigonelline is probably mediated by heterotrophic and mixotrophic micro-organisms capable of oxidising S 0 , since it is known that the addition of organic matter stimulates chemoheterotrophic micro-organisms and not chemoautotrophic micro-organisms.
  • the fertiliser composition of the invention is in powder form and comprises between 85 and 99.5% by weight of S 0 and between 0.5 and 15% of trigonelline.
  • the fertiliser composition of the invention comprises between 85 and 99.5% by weight of S 0 , between 0.5 and 10% by weight of trigonelline and between 0.5 and 5% by weight of other components selected from the group consisting of sugars, amino acids, organic acids, polyamines, alcohols, nucleotides and combinations thereof, the fertiliser composition being in the form of a watersoluble powder.
  • the presence of the other components included in the fertiliser composition of the invention is due to the fact that the presence of organic molecules favours the oxidation of inorganic sulphur (Cifuentes and Lindemann, 1993; Bharathi, 2008). Therefore, the presence of these components other than trigonelline and elemental sulphur in the composition of the invention is based on the fact that such components are organic molecules that can act as a source of energy or activate the metabolism of certain microorganisms.
  • sugars are preferably selected from mono- and di-saccharides such as sucrose, fructose, trehalose, glucose, arabinose, maltose, lactose, as well as mixtures thereof.
  • amino acids are preferably selected from threonine, lysine, phenylalanine, methionine, GABA, ornithine, glycine, aspartic acid, glutamine, glutamic acid, serine, asparagine, tyrosine, tryptophan, valine, leucine, isoleucine, proline, 4-hydroxyproline, arginine, histidine, alanine, cysteine, and mixtures thereof.
  • the organic acids are preferably selected from lactic acid, succinic acid, oxalic acid, gluconic acid, threonic acid, citric acid, fumaric acid and mixtures thereof.
  • Polyamines if present in the present composition, are preferably selected from putrescine, spermidine, spermine and mixtures thereof.
  • alcohols are preferably selected from glycerol, sorbitol, mannitol, myoinositol, as well as mixtures thereof.
  • the fertiliser composition of the invention is formulated in powder form, as indicated above, but can also be formulated as an emulsion composition with the addition of water or in granulated form by means of the addition of granulating agents known to the person skilled in the art.
  • the invention relates to a fertiliser composition such as the one described above in combination with an additional fertiliser selected from nitrogen fertilisers, phosphate fertilisers, potassium fertilisers, calcium fertilisers and amendments, micronutrients, boric acid, leonardite and feldspar, as well as combinations thereof, and/or in combination with one or more biostimulants selected from the group consisting of amino acid hydrolysates, humic extracts, seaweed extracts, live micro-organisms, extracts of micro-organisms and combinations thereof.
  • an additional fertiliser selected from nitrogen fertilisers, phosphate fertilisers, potassium fertilisers, calcium fertilisers and amendments, micronutrients, boric acid, leonardite and feldspar, as well as combinations thereof, and/or in combination with one or more biostimulants selected from the group consisting of amino acid hydrolysates, humic extracts, seaweed extracts, live micro-organisms, extracts of micro-organisms and combinations thereof.
  • the live micro-organisms or extracts of micro-organisms shall preferably be of the species Pichia guilliermondii , Azotobacter chroococcum , Bacillus megaterium , or other bacteria belonging to genera recognised for their elemental sulphur oxidising capacity, preferably Acidithiobacillus, Acidianus, Alicyclobacillus, Aquaspirillum, Bacillus, Beggiatoa, Mesorhizobium, Methylobacterium, Parococcus, Pseudomonas, Rhizobium, Starkeya, Thermithiobacillus, Thermothrix, Thiobacillus, Xanthobacter.
  • composition of the invention is present in the combination in a proportion of 10 to 60% by weight.
  • the additional nitrogen fertiliser is present in the blend in a proportion of 5 to 90 % by weight and is selected from urea, potassium nitrate, ammonium nitrate, calcium nitrate, calcium ammonium nitrate, nitromagnesium.
  • the additional phosphate fertiliser is present in the combination in a proportion of 5 to 90 % by weight and is selected from phosphate rock, triple superphosphate, single superphosphate, concentrated superphosphate, phosphoric acid.
  • the additional potassium fertiliser is present in the blend in a proportion of 5 to 90% by weight and is selected from potassium chloride and potassium hydroxide.
  • the additional calcium fertiliser is present in the blend in a proportion of 5 to 90% by weight and is selected from calcium chloride, calcium cyanamide, dolomite, limestone, calcium oxide, calcium hydroxide.
  • the additional micronutrient fertiliser is present in the combination in a proportion of 1 to 30% by weight and is selected from ammonium molybdate, cobalt chloride and micronutrients (iron, magnesium, zinc, manganese, copper) combined with complexing agents (humates, citrate, gluconates, heptagluconates and amino acids) or with chelating agents (ethylenediaminetetraacetic acid EDTA, ethylenediamine-di(o-hydroxyphenyl acetic acid) EDDHA, diethylenetriaminepentaacetic acid DPTA).
  • ammonium molybdate cobalt chloride and micronutrients (iron, magnesium, zinc, manganese, copper) combined with complexing agents (humates, citrate, gluconates, heptagluconates and amino acids) or with chelating agents (ethylenediaminetetraacetic acid EDTA, ethylenediamine-di(o-hydroxyphenyl acetic acid) EDDHA, diethylenetriaminep
  • boric acid as an additional fertiliser is present in the combination in a proportion of 1 to 30% by weight.
  • leonardite as an additional fertiliser is present in the combination in a proportion of 5 to 90% by weight.
  • feldspar as an additional fertiliser is present in the combination in a proportion of 5 to 90% by weight.
  • the biostimulants are present in the combination in a proportion of 5 to 90% by weight.
  • composition of the invention in powder or emulsion form, it is preferably applied in an amount of 0.5 to 20 kg/ha and 0.06 to 1 kg/ha respectively.
  • composition of the invention in combination with another additional fertiliser in granular form, preferably said combination is applied directly in an amount of 75 to 1,500 kg/ha.
  • Table 1 Days 0 10 20 30 40 50 80 Control - 0.24 0.27 0.25 0.24 0.23 0.2 0.2 S 0 0.24 0.37 0.51 0.73 0.87 0.93 1.02 S 0 +humic acids 0.24 0.69 1.17 1.54 1.88 2.01 2.15 S 0 +gluconic acid 0.24 0.81 1.28 1.75 2.07 2.18 2.23 S 0 +citric acid 0.24 0.75 1.25 1.68 1.95 2.21 2.28 S 0 +glutamic acid 0.24 1.15 2.15 2.87 3.21 3.27 3.39 S 0 +isoleucine 0.24 1.09 2.01 2.75 3.12 3.28 3.49 S 0 +leucine 0.24 1.18 2.2 3.08 3.46 3.82 3.97 S 0 +trigonellin e 0.24 1.45 2.69 3.97 4.45 4.68 5.02 S°+stachydrin 0.24 0.41 0.68 0.89 1.05 1.09 1.12 S 0 +glucose 0.24 0.95 1.79 2.54 2.84 2.
  • trigonelline is the molecule that increases S 0 oxidation the most, multiplying the SO 4 2- generated after 80 days by 2.3 with respect to humic acids.
  • Amino acids (leucine, isoleucine and glutamic acid), as well as glucose, fructose and glycerol also improve S 0 oxidation compared to humic acids.
  • Gluconic acid, citric acid and mannitol show similar results to humic acids.
  • Table 2 Days 0 10 20 30 40 50 80 Control - (ground) 0.24 0.27 0.25 0.24 0.23 0.2 0.2 Trigonelline 0.24 0.24 0.26 0.28 0.27 0.29 0.26 S 0 0.24 0.37 0.51 0.73 0.87 0.93 1.02 S 0 +trigonelline 0.24 1.45 2.69 3.97 4.45 4.68 5.02 Soil ster + S 0 0.24 0.23 0.23 0.27 0.25 0.28 0.26 Soilster + S 0 +trigonelline 0.24 0.25 0.24 0.28 0.29 0.3 0.31

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EP19935214.7A 2019-06-24 2019-06-24 Fertiliser composition including elemental sulfur and trigonelline as an enhancer of microbiological oxidation of elemental sulphur Active EP3970493B1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/ES2019/070441 WO2020260728A1 (es) 2019-06-24 2019-06-24 Composicion fertilizante que incluye azufre elemental y trigonelina como potenciador de la oxidacion microbiologica del s0

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EP3970493A1 EP3970493A1 (en) 2022-03-23
EP3970493A4 EP3970493A4 (en) 2023-01-04
EP3970493B1 true EP3970493B1 (en) 2023-10-25

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EP (1) EP3970493B1 (es)
BR (1) BR112021022452A2 (es)
ES (1) ES2964736T3 (es)
MA (1) MA55944B1 (es)
PT (1) PT3970493T (es)
WO (1) WO2020260728A1 (es)

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CR20220671A (es) * 2020-05-28 2023-03-28 Fertinagro Biotech Sl Procedimiento de fabricación industrial de fertilizantes granulados

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WO1984002059A1 (en) * 1982-11-26 1984-06-07 Manhattan College Method and composition for the promotion of leguminous plant productivity and seed yields
PL206943B1 (pl) * 2006-08-25 2010-10-29 Zak & Lstrok Ady Chemiczne Sia Nawóz siarkowy oraz sposób wytwarzania nawozu siarkowego granulowanego
MX2015009322A (es) * 2015-02-06 2016-11-28 Tiger-Sul Products (Canada) Co Composicion fertilizante a base de azufre con contenido de acido humico.

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MA55944A (fr) 2022-03-23
BR112021022452A2 (pt) 2022-01-04
ES2964736T3 (es) 2024-04-09
WO2020260728A1 (es) 2020-12-30
EP3970493A4 (en) 2023-01-04
PT3970493T (pt) 2023-12-11
MA55944B1 (fr) 2023-11-30
EP3970493A1 (en) 2022-03-23

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