EP3827080A4 - Microorganisms having increased lipid production, and compositions and methods of making and using the same - Google Patents

Microorganisms having increased lipid production, and compositions and methods of making and using the same Download PDF

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Publication number
EP3827080A4
EP3827080A4 EP19840843.7A EP19840843A EP3827080A4 EP 3827080 A4 EP3827080 A4 EP 3827080A4 EP 19840843 A EP19840843 A EP 19840843A EP 3827080 A4 EP3827080 A4 EP 3827080A4
Authority
EP
European Patent Office
Prior art keywords
microorganisms
compositions
making
methods
same
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP19840843.7A
Other languages
German (de)
French (fr)
Other versions
EP3827080A1 (en
Inventor
Eric R. MOELLERING
Tom Carlson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Viridos Inc
Original Assignee
Synthetic Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Synthetic Genomics Inc filed Critical Synthetic Genomics Inc
Publication of EP3827080A1 publication Critical patent/EP3827080A1/en
Publication of EP3827080A4 publication Critical patent/EP3827080A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01211—Long-chain-3-hydroxyacyl-CoA dehydrogenase (1.1.1.211)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/03—Oxidoreductases acting on the CH-CH group of donors (1.3) with oxygen as acceptor (1.3.3)
    • C12Y103/03006—Acyl-CoA oxidase (1.3.3.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/03—Oxo-acid-lyases (4.1.3)
    • C12Y401/03001—Isocitrate lyase (4.1.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01017—Enoyl-CoA hydratase (4.2.1.17), i.e. crotonase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
EP19840843.7A 2018-07-23 2019-07-22 Microorganisms having increased lipid production, and compositions and methods of making and using the same Withdrawn EP3827080A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862702253P 2018-07-23 2018-07-23
PCT/US2019/042768 WO2020023353A1 (en) 2018-07-23 2019-07-22 Microorganisms having increased lipid production, and compositions and methods of making and using the same

Publications (2)

Publication Number Publication Date
EP3827080A1 EP3827080A1 (en) 2021-06-02
EP3827080A4 true EP3827080A4 (en) 2022-04-20

Family

ID=69162608

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19840843.7A Withdrawn EP3827080A4 (en) 2018-07-23 2019-07-22 Microorganisms having increased lipid production, and compositions and methods of making and using the same

Country Status (7)

Country Link
US (1) US11046979B2 (en)
EP (1) EP3827080A4 (en)
CN (1) CN112384619A (en)
AU (1) AU2019310022A1 (en)
IL (1) IL280282A (en)
MX (1) MX2021000906A (en)
WO (1) WO2020023353A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2016293527B2 (en) * 2015-07-14 2022-01-13 Synthetic Genomics, Inc Microorganisms having increased lipid productivity
WO2024097353A1 (en) * 2022-11-04 2024-05-10 Viridos, Inc. Recombinant algae having high biomass and lipid productivity

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017011707A1 (en) * 2015-07-14 2017-01-19 Synthetic Genomics, Inc Microorganisms having increased lipid productivity
US20180186842A1 (en) * 2016-12-30 2018-07-05 Synthetic Genomics, Inc. High productivity algal mutants having reduced photosynthetic antenna

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012082731A2 (en) * 2010-12-13 2012-06-21 J. Craig Venter Institute Engineered microalgae with enhanced lipid production
US8709766B2 (en) * 2011-10-17 2014-04-29 Colorado School Of Mines Use of endogenous promoters in genetic engineering of Nannochloropsis gaditana
US11408007B2 (en) * 2014-09-26 2022-08-09 Yale University Compositions and methods for biocontainment of microorganisms
US11339399B2 (en) * 2014-12-31 2022-05-24 Viridos, Inc. Compositions and methods for high efficiency in vivo genome editing
US10689676B2 (en) * 2015-11-02 2020-06-23 Synthetic Genomics, Inc. Algal mutants with increased lipid productivity

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017011707A1 (en) * 2015-07-14 2017-01-19 Synthetic Genomics, Inc Microorganisms having increased lipid productivity
US20180186842A1 (en) * 2016-12-30 2018-07-05 Synthetic Genomics, Inc. High productivity algal mutants having reduced photosynthetic antenna

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ELISA CORTEGGIANI CARPINELLI ET AL: "Chromosome Scale Genome Assembly and Transcriptome Profiling of Nannochloropsis gaditana in Nitrogen Depletion", MOLECULAR PLANT, vol. 7, no. 2, 21 August 2013 (2013-08-21), pages 323 - 335, XP055222520, ISSN: 1674-2052, DOI: 10.1093/mp/sst120 *
IMAD AJJAWI ET AL: "Lipid production in Nannochloropsis gaditana is doubled by decreasing expression of a single transcriptional regulator", NATURE BIOTECHNOLOGY, vol. 35, no. 7, 19 June 2017 (2017-06-19), New York, pages 647 - 652, XP055540971, ISSN: 1087-0156, DOI: 10.1038/nbt.3865 *
See also references of WO2020023353A1 *

Also Published As

Publication number Publication date
US20200024623A1 (en) 2020-01-23
IL280282A (en) 2021-03-01
EP3827080A1 (en) 2021-06-02
US11046979B2 (en) 2021-06-29
CN112384619A (en) 2021-02-19
WO2020023353A1 (en) 2020-01-30
AU2019310022A1 (en) 2021-02-04
MX2021000906A (en) 2021-03-31

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