EP3712599A4 - Method for detecting and typing rare tumor cells in body fluid sample and kit therefor - Google Patents

Method for detecting and typing rare tumor cells in body fluid sample and kit therefor Download PDF

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Publication number
EP3712599A4
EP3712599A4 EP18879297.2A EP18879297A EP3712599A4 EP 3712599 A4 EP3712599 A4 EP 3712599A4 EP 18879297 A EP18879297 A EP 18879297A EP 3712599 A4 EP3712599 A4 EP 3712599A4
Authority
EP
European Patent Office
Prior art keywords
typing
detecting
tumor cells
body fluid
fluid sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP18879297.2A
Other languages
German (de)
French (fr)
Other versions
EP3712599C0 (en
EP3712599B1 (en
EP3712599A1 (en
Inventor
Qihui Shi
Yuliang Deng
Yin Tang
Zhuo Wang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hangzhou Junhui Biotechnology Co Ltd
Original Assignee
Suzhou Junhui Biotechnology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Suzhou Junhui Biotechnology Co Ltd filed Critical Suzhou Junhui Biotechnology Co Ltd
Publication of EP3712599A1 publication Critical patent/EP3712599A1/en
Publication of EP3712599A4 publication Critical patent/EP3712599A4/en
Application granted granted Critical
Publication of EP3712599C0 publication Critical patent/EP3712599C0/en
Publication of EP3712599B1 publication Critical patent/EP3712599B1/en
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Anticipated expiration legal-status Critical

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57407—Specifically defined cancers
    • G01N33/57423—Specifically defined cancers of lung
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1429—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its signal processing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G01N15/01—
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N2015/03—Electro-optical investigation of a plurality of particles, the analyser being characterised by the optical arrangement
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G01N2015/1022—
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70589—CD45
EP18879297.2A 2017-11-16 2018-10-24 Method for detecting and typing rare tumor cells in body fluid sample and kit therefor Active EP3712599B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201711139300.8A CN107917869B (en) 2017-11-16 2017-11-16 Method for detecting and typing rare tumor cells in body fluid sample and kit thereof
PCT/CN2018/111546 WO2019095943A1 (en) 2017-11-16 2018-10-24 Method for detecting and typing rare tumor cells in body fluid sample and kit therefor

Publications (4)

Publication Number Publication Date
EP3712599A1 EP3712599A1 (en) 2020-09-23
EP3712599A4 true EP3712599A4 (en) 2021-08-11
EP3712599C0 EP3712599C0 (en) 2024-02-28
EP3712599B1 EP3712599B1 (en) 2024-02-28

Family

ID=61895630

Family Applications (1)

Application Number Title Priority Date Filing Date
EP18879297.2A Active EP3712599B1 (en) 2017-11-16 2018-10-24 Method for detecting and typing rare tumor cells in body fluid sample and kit therefor

Country Status (4)

Country Link
US (1) US11047858B2 (en)
EP (1) EP3712599B1 (en)
CN (1) CN107917869B (en)
WO (1) WO2019095943A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107917869B (en) 2017-11-16 2020-06-19 苏州浚惠生物科技有限公司 Method for detecting and typing rare tumor cells in body fluid sample and kit thereof
CN111812323B (en) 2019-04-10 2022-04-12 杭州浚惠生物科技有限公司 Application of hexokinase 2 in detection of rare tumor cells in body fluid sample and kit
CN113567675B (en) * 2020-04-28 2023-09-22 苏州浚惠生物科技有限公司 Liquid biopsy companion diagnosis and kit for hexokinase 2 inhibitors of tumors
CN112147326B (en) * 2020-09-04 2022-04-08 北京大学 Accurate detection kit for tumor immune cell subset typing
CN112304816A (en) * 2020-10-28 2021-02-02 宁夏医科大学总医院 Cerebrospinal fluid cell characteristic collecting method and device
WO2024021040A1 (en) * 2022-07-29 2024-02-01 曾繁根 Cell identification method

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017020617A1 (en) * 2015-07-31 2017-02-09 上海交通大学 Method and device for detecting circulating tumor cell

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JP2002253298A (en) * 2001-03-02 2002-09-10 Wako Pure Chem Ind Ltd Rapid testing method for determining drug sensitivity
WO2003047331A2 (en) * 2001-12-04 2003-06-12 Nereus Pharmaceuticals,Inc. Methods and apparatus for cell based screening
JP4779115B2 (en) * 2005-12-16 2011-09-28 国立大学法人東北大学 Postoperative prognostic method for early lung cancer
CN105705088A (en) * 2013-03-15 2016-06-22 安派科生物医学科技（丽水）有限公司 Methods and apparatus for enhanced detection of diseases
CN104122254A (en) * 2014-01-23 2014-10-29 贵州大学 Dynamic measurement method for drug sensitivity of tumor cells
US20180231560A1 (en) * 2014-09-15 2018-08-16 Duke University Compositions and Methods for the Capture and Characterization of Circulating Tumor Cells
CN105954246B (en) * 2016-04-29 2020-10-30 上海乾翼生物科技有限公司 Method and kit for detecting free rare tumor cells in human biological fluid sample
CN107917869B (en) * 2017-11-16 2020-06-19 苏州浚惠生物科技有限公司 Method for detecting and typing rare tumor cells in body fluid sample and kit thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017020617A1 (en) * 2015-07-31 2017-02-09 上海交通大学 Method and device for detecting circulating tumor cell

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
SEPHRA N. RAMPERSAD: "Multiple Applications of Alamar Blue as an Indicator of Metabolic Function and Cellular Health in Cell Viability Bioassays", SENSORS, vol. 12, no. 12, 10 September 2012 (2012-09-10), pages 12347 - 12360, XP055367205, DOI: 10.3390/s120912347 *
SILVA FILOMENA S.G. ET AL: "Determination of Metabolic Viability and Cell Mass Using a Tandem Resazurin/Sulforhodamine B Assay : SRB/Resazurin Assay for Cell Viability and Metabolism", CURRENT PROTOCOLS IN TOXICOLOGY, vol. 68, no. 1, 1 May 2016 (2016-05-01), US, pages 2.24.1 - 2.24.15, XP055819331, ISSN: 1934-9254, DOI: 10.1002/cptx.1 *

Also Published As

Publication number Publication date
EP3712599C0 (en) 2024-02-28
EP3712599B1 (en) 2024-02-28
CN107917869B (en) 2020-06-19
WO2019095943A1 (en) 2019-05-23
CN107917869A (en) 2018-04-17
EP3712599A1 (en) 2020-09-23
US20200264180A1 (en) 2020-08-20
US11047858B2 (en) 2021-06-29

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