EP3674404A4 - Target sequence specific alteration technology using nucleotide target recognition - Google Patents

Target sequence specific alteration technology using nucleotide target recognition Download PDF

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Publication number
EP3674404A4
EP3674404A4 EP18847639.4A EP18847639A EP3674404A4 EP 3674404 A4 EP3674404 A4 EP 3674404A4 EP 18847639 A EP18847639 A EP 18847639A EP 3674404 A4 EP3674404 A4 EP 3674404A4
Authority
EP
European Patent Office
Prior art keywords
sequence specific
nucleotide
target
specific alteration
target sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP18847639.4A
Other languages
German (de)
French (fr)
Other versions
EP3674404A1 (en
Inventor
Keishi Osakabe
Yuriko OSAKABE
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Tokushima NUC
Original Assignee
University of Tokushima NUC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Tokushima NUC filed Critical University of Tokushima NUC
Publication of EP3674404A1 publication Critical patent/EP3674404A1/en
Publication of EP3674404A4 publication Critical patent/EP3674404A4/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)
EP18847639.4A 2017-08-21 2018-08-20 Target sequence specific alteration technology using nucleotide target recognition Pending EP3674404A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2017158876 2017-08-21
JP2017236518 2017-12-08
PCT/JP2018/030607 WO2019039417A1 (en) 2017-08-21 2018-08-20 Target sequence specific alteration technology using nucleotide target recognition

Publications (2)

Publication Number Publication Date
EP3674404A1 EP3674404A1 (en) 2020-07-01
EP3674404A4 true EP3674404A4 (en) 2021-05-26

Family

ID=65438898

Family Applications (1)

Application Number Title Priority Date Filing Date
EP18847639.4A Pending EP3674404A4 (en) 2017-08-21 2018-08-20 Target sequence specific alteration technology using nucleotide target recognition

Country Status (12)

Country Link
US (2) US12012596B2 (en)
EP (1) EP3674404A4 (en)
JP (2) JP7017259B2 (en)
KR (1) KR102626503B1 (en)
CN (1) CN111247247A (en)
AU (1) AU2018321021B2 (en)
BR (1) BR112020003439A2 (en)
CA (1) CA3073372A1 (en)
IL (1) IL272688A (en)
MX (1) MX2020001998A (en)
SG (1) SG11202001471SA (en)
WO (1) WO2019039417A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11807869B2 (en) * 2017-06-08 2023-11-07 Osaka University Method for producing DNA-edited eukaryotic cell, and kit used in the same
WO2020122104A1 (en) * 2018-12-11 2020-06-18 国立大学法人京都大学 Method for inducing deletion in genomic dna
WO2020184723A1 (en) * 2019-03-14 2020-09-17 国立大学法人徳島大学 Technique for modifying target sequence using crispr-type i-d system
US20220186263A1 (en) 2019-04-05 2022-06-16 Osaka University Method for producing knock-in cell
KR102550308B1 (en) * 2020-09-29 2023-06-30 경상국립대학교산학협력단 Method for producing genome-edited tomato plant with increased salt tolerance by SlHKT1;2 gene editing and genome-edited tomato plant with increased salt tolerance produced by the same method
JP7454881B2 (en) * 2020-10-08 2024-03-25 国立大学法人徳島大学 Target nucleotide sequence modification technology using CRISPR type ID system
CN112921038B (en) * 2021-02-23 2022-08-02 安徽农业大学 Homologous recombination mechanism mediated accurate sequence replacement gene editing method and element structure thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3636753A1 (en) * 2017-06-08 2020-04-15 Osaka University Method for manufacturing dna-edited eukaryotic cell, and kit used in method

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100102580A1 (en) 2008-10-23 2010-04-29 Brooks Ryan J Energy absorber with differentiating angled walls
GB201122458D0 (en) 2011-12-30 2012-02-08 Univ Wageningen Modified cascade ribonucleoproteins and uses thereof
CA2944978C (en) 2014-04-08 2024-02-13 North Carolina State University Methods and compositions for rna-directed repression of transcription using crispr-associated genes
EP3708671A1 (en) * 2014-06-06 2020-09-16 Regeneron Pharmaceuticals, Inc. Methods and compositions for modifying a targeted locus
CA2953499C (en) * 2014-06-23 2023-10-24 Regeneron Pharmaceuticals, Inc. Nuclease-mediated dna assembly
JP6780860B2 (en) 2015-09-09 2020-11-04 国立大学法人神戸大学 Genome sequence modification method that specifically converts the nucleobase of the targeted DNA sequence and the molecular complex used for it.
WO2017066497A2 (en) 2015-10-13 2017-04-20 Duke University Genome engineering with type i crispr systems in eukaryotic cells

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3636753A1 (en) * 2017-06-08 2020-04-15 Osaka University Method for manufacturing dna-edited eukaryotic cell, and kit used in method

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DATABASE UniProt [online] 5 September 2012 (2012-09-05), "SubName: Full=Uncharacterized protein {ECO:0000313|EMBL:CCI20996.1};", XP002802573, retrieved from EBI accession no. UNIPROT:I4HG22 Database accession no. I4HG22 *
DATABASE Uniprot [online] 5 September 2012 (2012-09-05), ANONYMOUS: "MICAD_3490011 - Uncharacterized protein - Microcystis aeruginosa PCC 7941 - MICAD_3490011 gene & protein", XP055792557, retrieved from https://www.uniprot.org/uniprot/I4GLE5 Database accession no. I4GLE5 *
MAKAROVA KIRA S ET AL: "SnapShot: Class 1 CRISPR-Cas Systems", CELL, ELSEVIER, AMSTERDAM NL, vol. 168, no. 5, 27 February 2017 (2017-02-27), pages 946, XP029932494, ISSN: 0092-8674, DOI: 10.1016/J.CELL.2017.02.018 *
MOHANRAJU PRARTHANA ET AL: "Diverse evolutionary roots and mechanistic variations of the CRISPR-Cas systems", SCIENCE, vol. 353, no. 6299, 4 August 2016 (2016-08-04), US, pages aad5147, XP055791785, ISSN: 0036-8075, Retrieved from the Internet <URL:https://science.sciencemag.org/content/353/6299/aad5147.full.pdf> DOI: 10.1126/science.aad5147 *
OSAKABE KEISHI ET AL: "Abstract", COMMUNICATIONS BIOLOGY, vol. 3, no. 1, 6 November 2020 (2020-11-06), XP055791658, Retrieved from the Internet <URL:http://www.nature.com/articles/s42003-020-01366-6> DOI: 10.1038/s42003-020-01366-6 *
See also references of WO2019039417A1 *

Also Published As

Publication number Publication date
KR20200039775A (en) 2020-04-16
JP7017259B2 (en) 2022-02-08
AU2018321021B2 (en) 2022-10-06
US20210363520A1 (en) 2021-11-25
EP3674404A1 (en) 2020-07-01
MX2020001998A (en) 2020-10-05
AU2018321021A1 (en) 2020-03-26
CN111247247A (en) 2020-06-05
CA3073372A1 (en) 2019-02-28
BR112020003439A2 (en) 2020-08-25
IL272688A (en) 2020-04-30
JP7054283B2 (en) 2022-04-13
WO2019039417A1 (en) 2019-02-28
KR102626503B1 (en) 2024-01-17
JPWO2019039417A1 (en) 2020-11-26
US20240327828A1 (en) 2024-10-03
JP2022009293A (en) 2022-01-14
US12012596B2 (en) 2024-06-18
NZ762361A (en) 2024-03-22
SG11202001471SA (en) 2020-03-30

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