EP3601574A4 - Engineered cascade components and cascade complexes - Google Patents

Engineered cascade components and cascade complexes Download PDF

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Publication number
EP3601574A4
EP3601574A4 EP19762697.1A EP19762697A EP3601574A4 EP 3601574 A4 EP3601574 A4 EP 3601574A4 EP 19762697 A EP19762697 A EP 19762697A EP 3601574 A4 EP3601574 A4 EP 3601574A4
Authority
EP
European Patent Office
Prior art keywords
cascade
engineered
complexes
components
cascade components
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP19762697.1A
Other languages
German (de)
French (fr)
Other versions
EP3601574A1 (en
Inventor
designation of the inventor has not yet been filed The
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Caribou Biosciences Inc
Original Assignee
Caribou Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US16/104,875 external-priority patent/US10227576B1/en
Application filed by Caribou Biosciences Inc filed Critical Caribou Biosciences Inc
Priority to EP22208929.4A priority Critical patent/EP4219720A3/en
Publication of EP3601574A1 publication Critical patent/EP3601574A1/en
Publication of EP3601574A4 publication Critical patent/EP3601574A4/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/21—Endodeoxyribonucleases producing 5'-phosphomonoesters (3.1.21)
    • C12Y301/21004—Type II site-specific deoxyribonuclease (3.1.21.4)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
EP19762697.1A 2018-06-13 2019-06-12 Engineered cascade components and cascade complexes Pending EP3601574A4 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP22208929.4A EP4219720A3 (en) 2018-06-13 2019-06-12 Engineered cascade components and cascade complexes

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201862684735P 2018-06-13 2018-06-13
US16/104,875 US10227576B1 (en) 2018-06-13 2018-08-17 Engineered cascade components and cascade complexes
US16/262,773 US10329547B1 (en) 2018-06-13 2019-01-30 Engineered cascade components and cascade complexes
US201962807717P 2019-02-19 2019-02-19
US16/420,061 US10457922B1 (en) 2018-06-13 2019-05-22 Engineered cascade components and cascade complexes
PCT/US2019/036864 WO2019241452A1 (en) 2018-06-13 2019-06-12 Engineered cascade components and cascade complexes

Related Child Applications (1)

Application Number Title Priority Date Filing Date
EP22208929.4A Division EP4219720A3 (en) 2018-06-13 2019-06-12 Engineered cascade components and cascade complexes

Publications (2)

Publication Number Publication Date
EP3601574A1 EP3601574A1 (en) 2020-02-05
EP3601574A4 true EP3601574A4 (en) 2020-03-18

Family

ID=68843611

Family Applications (2)

Application Number Title Priority Date Filing Date
EP19762697.1A Pending EP3601574A4 (en) 2018-06-13 2019-06-12 Engineered cascade components and cascade complexes
EP22208929.4A Pending EP4219720A3 (en) 2018-06-13 2019-06-12 Engineered cascade components and cascade complexes

Family Applications After (1)

Application Number Title Priority Date Filing Date
EP22208929.4A Pending EP4219720A3 (en) 2018-06-13 2019-06-12 Engineered cascade components and cascade complexes

Country Status (10)

Country Link
EP (2) EP3601574A4 (en)
JP (1) JP6965466B2 (en)
KR (2) KR20230035689A (en)
CN (1) CN112272704A (en)
AU (2) AU2019284926C1 (en)
CA (2) CA3102148A1 (en)
MX (1) MX2020013579A (en)
SG (1) SG11202010182SA (en)
WO (1) WO2019241452A1 (en)
ZA (1) ZA202006880B (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021252570A1 (en) * 2020-06-10 2021-12-16 North Carolina State University Novel type i-c crispr-cas system from clostridia
CN115369098A (en) * 2021-05-17 2022-11-22 中国科学院分子植物科学卓越创新中心 Novel CRISPR (clustered regularly interspaced short palindromic repeats) related transposase
US20230023791A1 (en) 2021-06-01 2023-01-26 Arbor Biotechnologies, Inc. Gene editing systems comprising a crispr nuclease and uses thereof
CN117529552A (en) * 2021-06-03 2024-02-06 中国农业大学 Type I-C CRISPR-Cas3 system and application thereof
CN115011581A (en) * 2022-04-14 2022-09-06 吉林大学 Method for enabling cas3 system to be knocked out accurately
CN117384867B (en) * 2022-09-16 2024-06-07 北京普译生物科技有限公司 Modified Cas3 translocation enzyme and application thereof
WO2024096790A1 (en) * 2022-10-31 2024-05-10 Soledits Ab Modular crispr system
CN116987699A (en) * 2023-09-05 2023-11-03 深圳市艾迪贝克生物医药有限公司 Gene fragment for preparing universal CAR-T cells, tool system and application thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013098244A1 (en) * 2011-12-30 2013-07-04 Wageningen Universiteit Modified cascade ribonucleoproteins and uses thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7446190B2 (en) 2002-05-28 2008-11-04 Sloan-Kettering Institute For Cancer Research Nucleic acids encoding chimeric T cell receptors
US7435596B2 (en) 2004-11-04 2008-10-14 St. Jude Children's Research Hospital, Inc. Modified cell line and method for expansion of NK cell
JP2016519652A (en) 2013-03-14 2016-07-07 カリブー・バイオサイエンシーズ・インコーポレイテッド Nucleic acid targeting nucleic acid compositions and methods
US20150044772A1 (en) * 2013-08-09 2015-02-12 Sage Labs, Inc. Crispr/cas system-based novel fusion protein and its applications in genome editing
MX2016007325A (en) * 2013-12-12 2017-07-19 Broad Inst Inc Compositions and methods of use of crispr-cas systems in nucleotide repeat disorders.
EP4089175A1 (en) * 2015-10-13 2022-11-16 Duke University Genome engineering with type i crispr systems in eukaryotic cells

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013098244A1 (en) * 2011-12-30 2013-07-04 Wageningen Universiteit Modified cascade ribonucleoproteins and uses thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
HOCHSTRASSER MEGAN L ET AL: "DNA Targeting by a Minimal CRISPR RNA-Guided Cascade", MOLECULAR CELL, ELSEVIER, AMSTERDAM, NL, vol. 63, no. 5, 1 September 2016 (2016-09-01), pages 840 - 851, XP029711874, ISSN: 1097-2765, DOI: 10.1016/J.MOLCEL.2016.07.027 *
MAKAROVA KIRA S ET AL: "SnapShot: Class 1 CRISPR-Cas Systems", CELL, ELSEVIER, AMSTERDAM, NL, vol. 168, no. 5, 27 February 2017 (2017-02-27), pages 946, XP029932494, ISSN: 0092-8674, DOI: 10.1016/J.CELL.2017.02.018 *

Also Published As

Publication number Publication date
AU2019284926A1 (en) 2020-11-05
EP4219720A3 (en) 2023-08-30
CA3163768A1 (en) 2019-12-19
KR20230035689A (en) 2023-03-14
SG11202010182SA (en) 2020-11-27
AU2022200903A1 (en) 2022-03-03
KR20210006966A (en) 2021-01-19
MX2020013579A (en) 2021-05-12
AU2022200903B2 (en) 2023-12-21
AU2019284926C1 (en) 2023-04-27
KR102516697B1 (en) 2023-03-31
CA3102148A1 (en) 2019-12-19
JP2021520844A (en) 2021-08-26
CN112272704A (en) 2021-01-26
ZA202006880B (en) 2021-10-27
WO2019241452A1 (en) 2019-12-19
EP4219720A2 (en) 2023-08-02
JP6965466B2 (en) 2021-11-10
EP3601574A1 (en) 2020-02-05
AU2019284926B2 (en) 2023-01-12

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