EP3549673A1 - Élément chauffant pour laboratoire sur puce - Google Patents

Élément chauffant pour laboratoire sur puce Download PDF

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Publication number
EP3549673A1
EP3549673A1 EP19167447.2A EP19167447A EP3549673A1 EP 3549673 A1 EP3549673 A1 EP 3549673A1 EP 19167447 A EP19167447 A EP 19167447A EP 3549673 A1 EP3549673 A1 EP 3549673A1
Authority
EP
European Patent Office
Prior art keywords
disc
lab
electrically conducting
temperature
conducting element
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP19167447.2A
Other languages
German (de)
English (en)
Inventor
Matja VENCELJ
Marko MAHNE
Tadej KOKALJ
Aman Russom
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut Jozef Stefan
Original Assignee
Institut Jozef Stefan
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Filing date
Publication date
Application filed by Institut Jozef Stefan filed Critical Institut Jozef Stefan
Publication of EP3549673A1 publication Critical patent/EP3549673A1/fr
Withdrawn legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
    • B01L7/5255—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones by moving sample containers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
    • B01L9/527—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips for microfluidic devices, e.g. used for lab-on-a-chip
    • H—ELECTRICITY
    • H05—ELECTRIC TECHNIQUES NOT OTHERWISE PROVIDED FOR
    • H05B—ELECTRIC HEATING; ELECTRIC LIGHT SOURCES NOT OTHERWISE PROVIDED FOR; CIRCUIT ARRANGEMENTS FOR ELECTRIC LIGHT SOURCES, IN GENERAL
    • H05B6/00—Heating by electric, magnetic or electromagnetic fields
    • H05B6/02—Induction heating
    • H05B6/10—Induction heating apparatus, other than furnaces, for specific applications
    • H05B6/105—Induction heating apparatus, other than furnaces, for specific applications using a susceptor
    • H05B6/108—Induction heating apparatus, other than furnaces, for specific applications using a susceptor for heating a fluid
    • H—ELECTRICITY
    • H05—ELECTRIC TECHNIQUES NOT OTHERWISE PROVIDED FOR
    • H05B—ELECTRIC HEATING; ELECTRIC LIGHT SOURCES NOT OTHERWISE PROVIDED FOR; CIRCUIT ARRANGEMENTS FOR ELECTRIC LIGHT SOURCES, IN GENERAL
    • H05B6/00—Heating by electric, magnetic or electromagnetic fields
    • H05B6/02—Induction heating
    • H05B6/10—Induction heating apparatus, other than furnaces, for specific applications
    • H05B6/105—Induction heating apparatus, other than furnaces, for specific applications using a susceptor
    • H05B6/109—Induction heating apparatus, other than furnaces, for specific applications using a susceptor using magnets rotating with respect to a susceptor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0803—Disc shape
    • B01L2300/0806—Standardised forms, e.g. compact disc [CD] format
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1811—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using electromagnetic induction heating
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1816—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using induction heating

Definitions

  • the present invention relates to a heater for a lab-on-a-chip technology, and specifically a heater utilizing magnetic induction. It also relates to systems and methods for heating and operating microfluidic chips.
  • the lab-on-a-disc was developed as a subsidiary to the growing lab-on-a-chip and microfluidics field. Both of these aim to increase the miniaturisation, portability, accuracy, and cost effectiveness of biochemical analysis with a particular focus on diagnostics.
  • Lab-on-a-disc has a high sample-to-result potential due to its simplicity and ease of integration of several protocol steps of biochemical analysis into a single disc.
  • PCR polymerase chain reaction
  • the reaction chamber temperature needs to be precisely controlled, and alternated between three temperatures (generally between 53°C and 95°C) in each cycle.
  • the most widely used heater for PCR systems is the Peltier heater, which uses the thermoelectric effect to convert electrical current into heat.
  • hot-air blowers, infrared light emitters, laser devices, and microwave emitters have been used as a heater in PCR systems.
  • more than 90% of the total time may be due to temperature cycling rather than the underlying amplification process.
  • An object of the present invention is to provide a heating mechanism for lab-on-a-disc applications, preferably one which provides for rapid cycling, precise real-time temperature measurement and control, simplicity, portability and/or cost efficiency.
  • the disclosure herein provides a heater for a lab-on-a-disc that operates through magnetic induction.
  • the invention provides a lab-on-a-disc, including:
  • Such a lab-on-a-disc can be inexpensively produced, and operable to heat to and maintain a present temperature with accuracy and speed.
  • the temperature can either be controlled via the control of the rotational frequency that is inherently integrated in lab-on-a-disc platforms or by adjusting the distance between the rotating lab-on-a-disc and the one or more magnets. No additional heaters are required for thermal cycling.
  • the heating energy is provided through the conversion of mechanical work of the driving motor.
  • the invention provides a system comprising:
  • the invention provides a method of controlling the temperature of a lab-on-a-disc, the lab-on-a-disc comprising a temperature sensor, a biochemical testing apparatus, and an electrically conducting element, the method including:
  • the invention provides a system comprising:
  • the invention provides a kit comprising:
  • the invention provides a heater attachment for a lab-on-a-disc, the heater attachment comprising:
  • the electrically conducting element may be formed of copper. It may be a foil or plate.
  • the element may be permanently fixed or releasably fixed to the lab-on-a-disc.
  • the lab-on-a-disc may include a temperature sensor, operable to monitor the temperature of one or more of: the biochemical testing apparatus (or a part thereof), or the electrically conducting element. Temperature control of the biochemical testing apparatus on the lab-on-a-disc is important for many operations. Sensing the temperature of the electrically conducting element may be used as a substitute for sensing the temperature of the biochemical testing apparatus as it may be easier to thermally couple the temperature sensor to the electrically conducting element.
  • the lab-on-a-disc may include a transmitter, which transmits the temperature sensed by the temperature sensor, for example to an external controller.
  • the transmitter may comprise one or more light emitting diodes, which turn on and off at a frequency which is dependent on the sensed temperature. This can provide a simple approach to transmitting the temperature data in a contactless way which does not require much power to implement.
  • the transmitter may be implemented as an RF transmitter, operable to communicate information to a receiver.
  • the lab-on-a-disc may include one or more turns of electrically conducting wire, connected to either or both of the temperature sensor and transmitter, such that in use the temperature sensor and/or transmitter are powered by current induced in the one or more turns of electrically conducting wire. This can reduce or remove the need for a battery or other energy source to be provided on the lab-on-a-disc to power the temperature sensor and/or transmitter.
  • the transmitter and/or sensor may be powered by a battery, or a capacitor.
  • the transmitter may be mounted to a communication disc, and the temperature sensor may be connected to the transmitter.
  • the communication disc may be separable from the lab-on-a-disc.
  • the transmitter may also be mounted directly on the lab-on-a-disc.
  • the lab-on-a-disc may include a centre spindle hole about which, in use, the lab-on-a-disc rotates.
  • the one or more magnets may be located in a plate fixed below the lab-on-a-disc. There may be a plurality of magnets. The plurality of magnets may be disposed equidistantly around a circumference of the plate. Adjacent magnets may have an opposite polarity. The magnets may be fixed or static magnets.
  • the one or more magnets may be mounted in a carrier which is itself rotatable relative to the lab-on-a-disc.
  • the relative speeds of rotation of the magnets and the lab-on-a-disc may be controlled so as to allow control of the heating effect from the relative rotation, whilst rotating the lab-on-a-disc at a speed which is required or desirable for operation of the biochemical testing apparatus.
  • the induced currents may be referred to as eddy currents.
  • the biochemical testing apparatus may be a reaction chamber.
  • the reaction chamber may be one suitable for PCR reactions and may have a volume of no more than 1000 ⁇ L and/or no less than 1 ⁇ L. In other arrangements the reaction chamber may have a volume of no more than 1 ⁇ L.
  • the system may further include a controller, which is configured to operate the motor in response to receiving a temperature sensed on the lab-on-a-disc.
  • the system may further include an optical sensor.
  • the optical sensor may be configured to sense the frequency with which the one or more light emitting diodes turn on and off, and thereby derive a temperature sensed by the temperature sensor.
  • the controller may use a closed control loop to control the motor.
  • the heater attachment may include a temperature sensor, for sensing the temperature of either the electrically conducting element(s) or, when attached to the lab-on-a-disc, the biochemical testing apparatus.
  • the heater attachment may include a transmitter as discussed above, for example a number of LEDs whose blink frequency is a function of the sensed temperature.
  • Figure 1 illustrates a cross-sectional schematic view of a system 100 according to an embodiment of the present invention.
  • a lab-on-a-disc 101 which may be for example a CD or DVD, and a communication disc 102 are mounted on a spindle attached to a motor 103. This allows both the lab-on-a-disc and the communication disc to be rotated around axis 104.
  • the motor may be a DC-driven electric motor, or an AC-driven electric motor.
  • the lab-on-a-disc 101 in this example includes a temperature sensor 105, an electrically conducting element 106 (here, a copper plate), and a biochemical testing apparatus (not shown in Figure 1 ).
  • the lab-on-a-disc is discussed in more detail below with respect to Figure 2 .
  • the temperature sensor is connected to the communication disc 102 via one or more wires, and in this example is a thermistor.
  • contacts on the communication disc may align with contacts on the lab-on-a-disc.
  • the components of the communication disc may be integrated with the lab-on-a-disc.
  • the communication disc is discussed in more detail below with respect to Figure 3 .
  • the magnet holder 107 Mounted below the lab-on-a-disc 101, i.e. between the lab-on-a-disc and the motor 103, is a magnet holder 107.
  • the magnet holder includes a plurality of magnets 108a - 108h (although it will be appreciated that any number of magnets, including a single magnet could be used).
  • the magnets may be neodymium (nickel plated) disc-shaped magnets with a diameter of 20 mm, a height of 10 mm, and a magnetic strength of around 110 Newton of pulling force.
  • a bearing 113 is used to (i) support the axial load from the lab-on-a-disc and stabilize a rotational axis of the motor against radial loads; and/or (ii) fix the position of the magnet holder 107 relative to the lab-on-a-disc.
  • the motor 103 and magnet holder 107 are mounted to a housing 109. Whilst the magnet holder is mounted between the motor and the lab-on-a-disc 101, it will be appreciated that alternatively the lab-on-a-disc could be placed between the magnet holder and the motor.
  • the housing 109 also includes safety protection 110 which encloses the lab-on-a-disc 101. Whilst in this example magnet holder is fixed relative to the housing, it will be appreciated that alternatively the magnetic holder may be mounted to an additional motor which is independent of the motor 103 connected to the lab-on-a-disc.
  • the motor 103 is connected to a controller 111, which operates to vary the speed of the motor and therefore the angular frequency of the lab-on-a-disc 101.
  • the motor may operate in accordance with a pre-programmed protocol, or may be instructable via an electrical interface or manual controls.
  • the controller 111 is coupled to an optical sensor 112.
  • the optical sensor senses light emitted from the communication disc 102, and from this light derives a temperature sensed by the temperature sensor 105.
  • the controller may vary the speed of the motor.
  • the speed of the motor By decreasing the speed of the motor, the angular frequency of the lab-on-a-disc 101 is also decreased. This reduction in angular frequency decreases the current induced in the electrically conducting element 106, which will decrease the temperature of the electrically conducting element.
  • increasing the speed of the motor will increase the angular frequency of the lab-on-a-disc. This increase in angular frequency increases the current induced in the electrically conducting element which increases the temperature of the electrically conducting element.
  • the controller may operate to move the magnet holder 107 relative to the lab-on-a-disc 101 to increase the distance therebetween. This also results in a decrease in the induced current and therefore a decrease in the temperature of the electrically conducting element 106. Conversely, the distance between the magnet holder and the lab-on-a-disc may be decreased which will increase the induced current and therefore increase the temperature of the electrically conducting element.
  • Figure 2 shows a top-down schematic view of the lab-on-a-disc 101 shown in Figure 1 .
  • the lab-on-a-disc is a generally disc shaped object with a number of elements mounted to or in it.
  • the disc may be, for example, a CD or DVD which has been modified.
  • the dimensions of the lab-on-a-disc may substantively match the CD or DVD standard and so have a diameter of 120 mm and a thickness of 1.2 mm.
  • the lab-on-a-disc contains a spindle hole 201, which may have a diameter of 15 mm.
  • the lab-on-a-disc 101 includes the electrically conducting element 105 described previously. As can be seen more clearly in this Figure, the electrically conducting element in this example is a copper plate.
  • the temperature sensor 106 is positioned on top of the electrically conducting element. However in some examples it may be located adjacent (i.e. offset in a top-down view) to the electrically conducting element.
  • the lab-on-a-disc also includes a biochemical testing apparatus 202, which is adjacent to the electrically conducting element. Therefore, when the electrically conducting element heats (due to induced currents) the temperature of the biochemical testing apparatus will also increase.
  • Figure 3 shows a top-down schematic view of the magnet holder 107 discussed above.
  • the magnet holder in this example includes eight fixed magnets: 108a-108h, disposed equidistantly around a circumference of the magnet holder. As can be seen, adjacent magnets are of an opposite polarity. The magnets are disposed at the same radial distance from a centre of the holder, and periodically around the circumference corresponding to that radial distance.
  • the magnetic holder like the lab-on-a-disc, may have substantially the same dimensions as a CD or DVD. However, it will of course be appreciated that the magnet holder may take virtually any shape.
  • FIG 4 shows an electronic circuit 400 diagram of a transmitter as may be included in the communication disc 102.
  • the transmitter which may be referred to as a thermistor-based temperature beacon, broadly comprises: a switch 401; an oscillator 402; LEDs 403a - 403c; and a power source 404.
  • the switch 401 in this example is an automatic power switch which closes when the communication disc is rotating. This can help to conserve the energy stored in the battery/capacitor when the disc is not rotating.
  • the oscillator 402 may be provided as a 555 series timer IC, in a stable topology which uses the thermistor 106 of the lab-on-a-disc 101 as the charge-discharge resistor.
  • the oscillator may be tuned to between 1 and 5 kHz at room temperature (between 20°C and 25°C).
  • the range of tuned frequencies allows the desired precision on a circa 1s time scale for measurement. Higher frequencies are not generally required, and may radiate electromagnetic interference.
  • the LEDs 403a-403c in this example share a cathode.
  • the power source 404 may be either (i) a battery, e.g. a 3V lithium button cell; (ii) a capacitor or super capacitor; or (iii) a number of turns of electrically conducting wire which inductively harvest power when the communication disc is rotated.
  • the resistance of the thermistor 106 varies the frequency of the output of the oscillator 402. This output is provided to the LEDs 403a - 403c, causing them to flash (i.e. turn on and off) at the same frequency. Light is thus used to transmit the temperature sensed by the thermistor as a frequency of flashing.
  • an RF transmitter may be included in the circuit 400 and used to transmit information (including the sensed temperature) to a receiver.
  • Figure 5 shows an implementation of the communication disc 102 including the electronic circuit 400. Like features are indicated by like reference numerals. Notably, it can be seen that the LEDs 403a-403c are disposed circumferentially around a common radius of the communication disc 102. Also, the contacts 501 for connection to the thermistor can be seen as the thermistor in this example is provided on the lab-on-a-disc 101.
  • the communication disc 102 may be powered by, for example, 20 turns of electrically conducting wire. At around 2000 RPM, at least 2.5 V of induced voltage can be provided by the 20 turns of wire. This setup allows all components of the communication disc to be integrated into the disc, whereas if a battery is provided this cannot be integrated into the disc and a separate battery holder may be needed as shown in Figure 4 .
  • the communication disc 102 may convey the output of these to a controller.
  • a fluorescent fingerprint may be sensed by a sensor on the lab-on-a-disc and provided to the controller.
  • the communication disc may provide a structured serial protocol for the transfer of information.
  • Figure 6 shows a circuit diagram 600 suitable for detecting the light emitted from the LEDs 403a - 403c of the communication disc, and converting it into a frequency encoded temperature signal suitable for microcontroller readout.
  • a circuit diagram 600 suitable for detecting the light emitted from the LEDs 403a - 403c of the communication disc, and converting it into a frequency encoded temperature signal suitable for microcontroller readout.
  • it includes a photodiode which provides a voltage to a gain stage in response to detecting light from the LEDs.
  • Figure 7 is a plot of asymptotic temperature (i.e. the temperature measured after a given period of time, when the temperature stabilizes and is no longer influenced by transition phenomena) against rotations per minute (RPM). As can be seen, the temperature linearly follows the rotation frequency of the disc. At around 1500 RPM, the highest temperature needed for PCR is achieved (around 95°C). With a further increase to 2000 RPM, a temperature over 120°C can be reached.
  • Figure 8 is an experimental plot of temperature against time. As can be seen, a temperature of around 90°C was be attained within a few (1 - 5) seconds. The rate of temperature increase seen at around 50 seconds is around 5°C/s. The temperature was maintained for between 30 and 50 seconds, before passive cooling was used by decreasing the angular frequency of the lab-on-a-disc 101. A cooling rate of around 1°C/s was achieved at the interval of thermal cycling temperatures required for a PCR protocol.
  • the lab-on-a-disc 101 includes a biochemical testing apparatus.
  • the testing apparatus may be usable for amplification and detection of nucleic acids.
  • the testing apparatus may be used for PCR or loop-mediated amplification protocols (LAMP).
  • LAMP loop-mediated amplification protocols
  • the biochemical testing apparatus may also be used to induce endothermic reactions on the lab-on-a-disc, or to induce biological processes where elevated temperature is needed e.g. cell lysis, or thermoporation.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Electromagnetism (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating Or Analyzing Materials By The Use Of Magnetic Means (AREA)
EP19167447.2A 2018-04-05 2019-04-04 Élément chauffant pour laboratoire sur puce Withdrawn EP3549673A1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB1805611.9A GB2572606A (en) 2018-04-05 2018-04-05 Heater for lab-on-a-chip

Publications (1)

Publication Number Publication Date
EP3549673A1 true EP3549673A1 (fr) 2019-10-09

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EP (1) EP3549673A1 (fr)
GB (1) GB2572606A (fr)
SI (1) SI25635A (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114774264A (zh) * 2022-06-22 2022-07-22 至美时代生物智能科技(北京)有限公司 一种空气微生物自动化检测系统
WO2023110010A1 (fr) 2021-12-16 2023-06-22 Dermagnostix GmbH Biopuce microfluidique centrifuge

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6593143B1 (en) * 2000-02-29 2003-07-15 Agilent Technologies, Inc. Centrifuge system with contactless regulation of chemical-sample temperature using eddy currents
EP1681553A2 (fr) * 2005-01-17 2006-07-19 Hitachi High-Technologies Corporation Appareil d'analyse chimique et cartouche d'analyse chimique
US20080308160A1 (en) * 2005-05-06 2008-12-18 Applera Corporation Device including inductively heatable fluid retainment region, and method
WO2017085472A1 (fr) * 2015-11-16 2017-05-26 Mast Group Limited Appareil de réalisation d'essai

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2556626A (en) * 2016-11-16 2018-06-06 Dublin Institute Of Tech A microfluidic device

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6593143B1 (en) * 2000-02-29 2003-07-15 Agilent Technologies, Inc. Centrifuge system with contactless regulation of chemical-sample temperature using eddy currents
EP1681553A2 (fr) * 2005-01-17 2006-07-19 Hitachi High-Technologies Corporation Appareil d'analyse chimique et cartouche d'analyse chimique
US20080308160A1 (en) * 2005-05-06 2008-12-18 Applera Corporation Device including inductively heatable fluid retainment region, and method
WO2017085472A1 (fr) * 2015-11-16 2017-05-26 Mast Group Limited Appareil de réalisation d'essai

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023110010A1 (fr) 2021-12-16 2023-06-22 Dermagnostix GmbH Biopuce microfluidique centrifuge
DE102021133494A1 (de) 2021-12-16 2023-06-22 Dermagnostix GmbH Centrifugal Microfluidic Biochip
DE102021133494B4 (de) 2021-12-16 2024-02-29 Dermagnostix GmbH Centrifugal Microfluidic Biochip
CN114774264A (zh) * 2022-06-22 2022-07-22 至美时代生物智能科技(北京)有限公司 一种空气微生物自动化检测系统

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