EP3500684A4 - Optically-based nanopore sequencing using quenching agents - Google Patents

Optically-based nanopore sequencing using quenching agents Download PDF

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Publication number
EP3500684A4
EP3500684A4 EP17841831.5A EP17841831A EP3500684A4 EP 3500684 A4 EP3500684 A4 EP 3500684A4 EP 17841831 A EP17841831 A EP 17841831A EP 3500684 A4 EP3500684 A4 EP 3500684A4
Authority
EP
European Patent Office
Prior art keywords
optically
nanopore sequencing
quenching agents
based nanopore
quenching
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP17841831.5A
Other languages
German (de)
French (fr)
Other versions
EP3500684A1 (en
Inventor
Stephen C. Macevicz
Steven Menchen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Quantapore Inc
Original Assignee
Quantapore Inc
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Filing date
Publication date
Application filed by Quantapore Inc filed Critical Quantapore Inc
Publication of EP3500684A1 publication Critical patent/EP3500684A1/en
Publication of EP3500684A4 publication Critical patent/EP3500684A4/en
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
EP17841831.5A 2016-08-19 2017-07-25 Optically-based nanopore sequencing using quenching agents Withdrawn EP3500684A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662377409P 2016-08-19 2016-08-19
PCT/US2017/043772 WO2018034807A1 (en) 2016-08-19 2017-07-25 Optically-based nanopore sequencing using quenching agents

Publications (2)

Publication Number Publication Date
EP3500684A1 EP3500684A1 (en) 2019-06-26
EP3500684A4 true EP3500684A4 (en) 2020-02-26

Family

ID=61196974

Family Applications (1)

Application Number Title Priority Date Filing Date
EP17841831.5A Withdrawn EP3500684A4 (en) 2016-08-19 2017-07-25 Optically-based nanopore sequencing using quenching agents

Country Status (6)

Country Link
EP (1) EP3500684A4 (en)
JP (1) JP2019528065A (en)
CN (1) CN109804087A (en)
AU (1) AU2017312746A1 (en)
CA (1) CA3031812A1 (en)
WO (1) WO2018034807A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018034807A1 (en) * 2016-08-19 2018-02-22 Quantapore, Inc. Optically-based nanopore sequencing using quenching agents
EP4047098A4 (en) * 2019-08-20 2023-06-07 EGI Tech (Shen Zhen) Co., Limited Method for sequencing polynucleotides on basis of optical signal dynamics of luminescent label and secondary luminescent signal
US20220364991A1 (en) * 2021-03-24 2022-11-17 Northeastern University Method and System for Decoding Information Stored on a Polymer Sequence

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Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120270221A1 (en) * 2006-05-12 2012-10-25 Pronucleotein Biotechnologies, Llc Methods of sequencing fluorophore-quencher FRET-aptamers
US20130203050A1 (en) * 2009-09-30 2013-08-08 Quantapore, Inc. Hybrid nanopore device with optical detection and methods of using same
US20160122812A1 (en) * 2014-10-10 2016-05-05 Quantapore, Inc. Nanopore-based polymer analysis with mutally-quenching fluorescent labels
WO2016065339A1 (en) * 2014-10-24 2016-04-28 Quantapore, Inc. Efficient optical analysis of polymers using arrays of nanostructures
WO2017180319A1 (en) * 2016-04-14 2017-10-19 Quantapore, Inc. Mixed optical signals in polymer analysis with nanopores
WO2018034807A1 (en) * 2016-08-19 2018-02-22 Quantapore, Inc. Optically-based nanopore sequencing using quenching agents

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO2018034807A1 *

Also Published As

Publication number Publication date
WO2018034807A1 (en) 2018-02-22
AU2017312746A1 (en) 2019-01-31
CA3031812A1 (en) 2018-02-22
JP2019528065A (en) 2019-10-10
CN109804087A (en) 2019-05-24
EP3500684A1 (en) 2019-06-26

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