EP3320340A1 - Procédés de génération de gradient de concentration de ph/ionique à proximité de surfaces d'électrode pour la modulation d'interactions biomoléculaires - Google Patents

Procédés de génération de gradient de concentration de ph/ionique à proximité de surfaces d'électrode pour la modulation d'interactions biomoléculaires

Info

Publication number
EP3320340A1
EP3320340A1 EP16735628.6A EP16735628A EP3320340A1 EP 3320340 A1 EP3320340 A1 EP 3320340A1 EP 16735628 A EP16735628 A EP 16735628A EP 3320340 A1 EP3320340 A1 EP 3320340A1
Authority
EP
European Patent Office
Prior art keywords
biosensor
electrode
solution
immobilized
electrodes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP16735628.6A
Other languages
German (de)
English (en)
Inventor
Rajan Gangadharan
Piyush Verma
Christopher Johnson
Sam Kavusi
Aldrich Lau
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Robert Bosch GmbH
Original Assignee
Robert Bosch GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US14/792,569 external-priority patent/US9874538B2/en
Application filed by Robert Bosch GmbH filed Critical Robert Bosch GmbH
Publication of EP3320340A1 publication Critical patent/EP3320340A1/fr
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
    • G01N27/3274—Corrective measures, e.g. error detection, compensation for temperature or hematocrit, calibration
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3277—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a redox reaction, e.g. detection by cyclic voltammetry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3278—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction involving nanosized elements, e.g. nanogaps or nanoparticles

Abstract

La présente invention concerne un dispositif et des procédés à utiliser dans un biocapteur comportant un réseau multisite de sites de test, ledit dispositif et lesdits procédés étant utilisés pour moduler les interactions de liaison entre une sonde (biomoléculaire) ou un agent de détection et un analyte d'intérêt par modulation du gradient de pH ou ionique à proximité des électrodes dans un tel biocapteur. L'invention porte en outre sur un agent électrochimiquement actif qui est approprié pour être utilisé dans des tampons biologiques pour modifier le pH des tampons biologiques. L'invention concerne également un procédé permettant de modifier le pH de tampons biologiques à l'aide des agents électrochimiquement actifs. L'invention a également trait à des procédés de modulation des interactions de liaison figurant dans un biocapteur, à des procédés d'analyse pour réguler et mesurer plus précisément le gradient du pH ou ionique à proximité des électrodes dans un tel biocapteur, et à des procédés d'analyse permettant de mesurer plus précisément un analyte d'intérêt dans un échantillon biologique.
EP16735628.6A 2015-07-06 2016-07-01 Procédés de génération de gradient de concentration de ph/ionique à proximité de surfaces d'électrode pour la modulation d'interactions biomoléculaires Pending EP3320340A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14/792,569 US9874538B2 (en) 2012-07-06 2015-07-06 Methods for generating pH/ionic concentration gradient near electrode surfaces for modulating biomolecular interactions
PCT/EP2016/065564 WO2017005645A1 (fr) 2015-07-06 2016-07-01 Procédés de génération de gradient de concentration de ph/ionique à proximité de surfaces d'électrode pour la modulation d'interactions biomoléculaires

Publications (1)

Publication Number Publication Date
EP3320340A1 true EP3320340A1 (fr) 2018-05-16

Family

ID=56360385

Family Applications (1)

Application Number Title Priority Date Filing Date
EP16735628.6A Pending EP3320340A1 (fr) 2015-07-06 2016-07-01 Procédés de génération de gradient de concentration de ph/ionique à proximité de surfaces d'électrode pour la modulation d'interactions biomoléculaires

Country Status (2)

Country Link
EP (1) EP3320340A1 (fr)
WO (1) WO2017005645A1 (fr)

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6753312B2 (en) 2001-07-12 2004-06-22 Cyvex Technologies, Inc. Food products and dietary supplements containing phenolated proteins and process for preparing the same
CA2672315A1 (fr) 2006-12-14 2008-06-26 Ion Torrent Systems Incorporated Procedes et appareil permettant de mesurer des analytes en utilisant des matrices de tec a grande echelle
US9063138B2 (en) 2009-10-15 2015-06-23 Robert Bosch Gmbh Device and method for indirect electronic condition control in biomolecule detection platforms
US9927435B2 (en) 2009-10-15 2018-03-27 Robert Bosch Gmbh Multisite biosensor and associated method
US9810688B2 (en) * 2013-03-15 2017-11-07 Robert Bosch Gmbh Smart glass slide for microarrays
US9075041B2 (en) 2012-07-06 2015-07-07 Robert Bosch Gmbh Methods for generating pH/ionic concentration gradient near electrode surfaces for modulating biomolecular interactions

Also Published As

Publication number Publication date
WO2017005645A1 (fr) 2017-01-12

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