EP3310342A1 - Inorganic controlled release particles with fast drug loading - Google Patents
Inorganic controlled release particles with fast drug loadingInfo
- Publication number
- EP3310342A1 EP3310342A1 EP16812105.1A EP16812105A EP3310342A1 EP 3310342 A1 EP3310342 A1 EP 3310342A1 EP 16812105 A EP16812105 A EP 16812105A EP 3310342 A1 EP3310342 A1 EP 3310342A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- nanoparticles
- microaggregate
- nanoparticle
- pores
- mmol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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- NLVFBUXFDBBNBW-PBSUHMDJSA-N tobramycin Chemical compound N[C@@H]1C[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N NLVFBUXFDBBNBW-PBSUHMDJSA-N 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
- A61K9/51—Nanocapsules; Nanoparticles
- A61K9/5107—Excipients; Inactive ingredients
- A61K9/5115—Inorganic compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/69—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
- A61K47/6921—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere
- A61K47/6923—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere the form being an inorganic particle, e.g. ceramic particles, silica particles, ferrite or synsorb
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
Definitions
- the present disclosure concerns mesoporous nanoparticles that can be used to deliver pharmaceutical agents, as well as materials that are formed from such nanoparticles.
- Silica gel obtained by sol-gel processing is an inorganic polymer produced synthetically by controlled hydrolysis and condensation of alkoxysilanes. This material, originally developed for engineering applications, is currently also being studied as a material for the entrapment and conversely the sustained release of bioactive compounds in laboratories worldwide. Important benefits associated with sol gel processing of bioactive ceramics and glasses are the excellent biocompatibility, as demonstrated in vivo (Radin, S., G. El-Bassyouni, E.J. Vresilovic, E. Schepers, and P. Ducheyne, In vivo tissue response to resorbable silica xerogels as contr oiled-release materials.
- Mesoporous materials are characterized by their large surface area, pore volume and pore size, and their narrow pore size distribution. For this reason, applications in the fields of catalysis, lasers, sensors, solar cells and so forth have been proposed and/or developed (S. Bhattacharyya, H.
- mesoporous silica nanoparticles can be internalized efficiently into cells, are nontoxic without affecting cell viability, growth, and differentiation, can escape from endo-lysosomal vesicles, and resist lysosomal degradation ⁇ Mai, N. K., et al ). It has also been demonstrated that these particles are capable of intracellular delivery of DNA ⁇ Zhao YN, et al., Capped mesoporous silica nanoparticles as stimuli-responsive controlled release systems for intracellular drug/gene delivery.
- none of the existing systems are capable of fast absorption of single or multiple drug molecules, while providing long-term controlled release immediately after absorption. Moreover, none of the above mentioned systems avoid initial burst release if the drug solution is hand mixed with MSNs or MSMPs and put in a release solution after only a short period of mixing. Most existing systems are, in fact, developed for intracellular delivery of therapeutics, where controlled release is not necessary once the mesoporous silica nanoparticle penetrates into the cell.
- nanoparticles comprising an inorganic material comprising a plurality of pores of which at least 90% have a diameter of 2 to 50 nm, the nanoparticle being surface-functionalized with a plurality of aldehyde groups that are present in an amount of 0.1 to 1.0 mmol/g.
- microaggregates comprising a plurality of nanoparticles aggregated together, each of the nanoparticles comprising an inorganic material comprising a plurality of pores of which at least 90% have a diameter of 2 to 50 nm; and, the nanoparticles being surface-functionalized with a plurality of aldehyde groups that are present in an amount of 0.1 to 1.0 mmol/g.
- the present disclosure also provides methods for making an aldehyde surface- functionalized mesoporous nanoparticle comprising synthesizing the nanoparticle using a single pot reaction procedure, wherein said procedure results in the nanoparticle being surface- functionalized with a plurality of aldehyde groups that are present in an amount of 0.1 to 1.0 mmol/g.
- Also provided are methods for loading a microaggregate with a pharmaceutical agent comprising contacting a solution comprising the pharmaceutical agent with the microaggregate for a duration of time that is sufficient to load the microaggregate with the pharmaceutical agent, the microaggregate comprising a plurality of nanoparticles aggregated together, each of the nanoparticles comprising an inorganic material comprising a plurality of pores of which at least 90% have a diameter of 2 to 50 nm; and, the nanoparticles being surface- functionalized with a plurality of aldehyde groups that are present in an amount of 0.1 to 1.0 mmol/g, wherein less than about 30 minutes of the contacting are required in order to load at least some of the pores of the nanoparticles with the pharmaceutical agent.
- the present disclosure also relates to methods for administering a
- the pharmaceutical agent to a subject comprising contacting the subject with a microaggregate, the microaggregate comprising a plurality of nanoparticles aggregated together, each of the nanoparticles comprising an inorganic material comprising a plurality of pores of which at least 90% have a diameter of 2 to 50 nm, at least some of the pores of the nanoparticle being loaded with the pharmaceutical agent; and, the nanoparticles being surface-functionalized with a plurality of aldehyde groups that are present in an amount of 0.1 to 1.0 mmol/g.
- FIG. 1 is a scanning electron micrograph of microaggregates according to the present disclosure.
- FIG. 2A and FIG. 2B depict individual mean cumulative release of vancomycin and rifampin, respectively, from inventive microaggregates as a function of immersion time in PBS.
- FIG. 3 illustrates the simultaneous release of vancomycin and rifampin from a single microaggregate as a function of immersion time in PBS.
- FIG. 4A shows the bactericidal activity of microaggregates that are loaded 10% vancomycin, are loaded with both 10% vancomycin and 10% rifampin, or do not contain any drugs, on the first day of exposure to a solution containing 10 4 CFU/mL of MRS A.
- FIG. 4B shows the bacterial activity of the microaggregates on days 2-4.
- treatment includes preventative (e.g., prophylactic), curative, or palliative treatment.
- preventative e.g., prophylactic
- curative e.g., palliative
- palliative treatment may be full or partial. For example, complete elimination of unwanted symptoms, or partial elimination of one or more unwanted symptoms would represent “treatment” as contemplated herein.
- the term "effective amount” refers to an amount effective, at dosages, and for periods of time necessary, to achieve the desired result with respect to the treatment of the relevant disorder, condition, or side effect. It will be appreciated that the effective amount of components of the present invention will vary from patient to patient not only with the particular compound, component or composition selected, the route of administration, and the ability of the components to elicit a desired response in the individual, but also with factors such as the disease state or severity of the condition to be alleviated, hormone levels, age, sex, weight of the individual, the state of being of the patient, and the severity of the condition being treated, concurrent medication or special diets then being followed by the particular patient, and other factors which those skilled in the art will recognize, with the appropriate dosage ultimately being at the discretion of the attendant physician.
- Dosage regimens may be adjusted to provide the improved therapeutic response.
- An effective amount is also one in which any toxic or detrimental effects of the components are outweighed by the therapeutically beneficial effects.
- the materials useful in the methods of the present invention are administered at a dosage and for a time such that the level of the undesired pathogen or symptom is reduced as compared to the level before the start of treatment.
- “Pharmaceutically acceptable” refers to those compounds, materials, compositions, and/or dosage forms which are, within the scope of sound medical judgment, suitable for contact with the tissues of human beings and animals without excessive toxicity, irritation, allergic response, or other problem complications commensurate with a reasonable benefit/risk ratio.
- the present disclosure relates to inorganic mesoporous materials that can quickly absorb pharmaceutical moieties and provide controlled release of such moieties over time.
- Previous work has included functionalization of the surface of mesoporous silica nanoparticles in order to regulate the release of drug molecules from the pores of the nanoparticles, the time required to introduce the drug molecules into the pores roughly correlated to the amount of time over which release occurred. That is, although such materials could provide extended release of a therapeutic molecule, loading of these materials with the therapeutic molecule required exposure of the latter to the former for a correspondingly extended period of time. This requirement meant that the drug loading process typically occurred well prior to the time of intended use of the loaded particles.
- nanoparticles and microparticles provide the unprecedented benefit of rapid, complete absorption of one or more drug moieties, coupled with subsequent controlled release.
- a medical practitioner can choose one or more pharmaceutical materials essentially contemporaneously with time of intended use, can load the inventive particles with the chosen pharmaceutical materials at the facility where treatment is to take place, and can deliver the drug- loaded particles to the treatment site, all within a very brief time frame.
- This characteristic greatly facilitates, for example, surgical planning and intraoperative decision-making, by permitting the surgeon to select a drug regimen on an intraoperative basis, and thereby at such time to load the chosen pharmaceutical or biological material and administer the loaded particles to the surgical site.
- the particles provide long term, controlled release.
- nanoparticles comprising an inorganic material comprising a plurality of pores, the nanoparticle being surface-functionalized with a plurality of aldehyde groups that are present in an amount of 0.1 to 1.0 mmol/g.
- the inorganic material may be, for example, silica, calcium phosphate, hydroxyapatite, alumina, titanium dioxide, or any combination thereof.
- the term "nanoparticle” preferably refers to a particle that does not include any physical dimension that exceeds 1000 nm.
- the nanoparticles may have at least one dimension that is 100-1000 nm, at least one dimension that is 100-500 nm, or at least one dimension that is 200-500 nm.
- the nanoparticles may therefore have at least one dimension that is about 100 nm, about 150 nm, about 200 nm, about 250 nm, about 300 nm, about 350 nm, about 400 nm, about 450 nm, about 500 nm, about 550 nm, about 600 nm, about 650 nm, about 700 nm, about 750 nm, about 800 nm, about 850 nm, about 900 nm, about 950 nm or about 1000 nm.
- the pores of a given nanoparticle will fall within a certain size distribution.
- at least 50%, at least 60%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, or at least 95% of the pores have a diameter of 2 to 50 nm.
- the nanoparticles comprise a plurality of pores of which at least 90% have a diameter of 2 to 50 nm, and the nanoparticles are surface-functionalized with a plurality of aldehyde groups that are present in an amount of 0.1 to 1.0 mmol/g.
- the present nanoparticles may be characterized as having a large surface area.
- the surface area of an inventive nanoparticle may be at least or about 500 m 2 /g, at least or about 600 m 2 /g, at least or about 700 m 2 /g, at least or about 800 m 2 /g, at least or about 900 m 2 /g, at least or about 1000 m 2 /g, at least or about 1100 m 2 /g, at least or about 1200 m 2 /g, at least or about 1300 m 2 /g, at least or about 1400 m 2 /g, or at least or about 1500 m 2 /g.
- the nanoparticles are surface-functionalized with a plurality of aldehyde groups (-CHO) that are present in an amount of 0.1 to 1.0 mmol/g.
- the aldehyde groups are bound directly to the inorganic material of which the nanoparticles are made.
- Previous mesoporous materials were functionalized with aldehyde groups in order to enable covalent attachment to other molecules, such as polymer (e.g., poly(methyl methacrylate), polyethylene glycol), metallic particles, or enzymes (e.g., lysozyme, penicillin G acylase).
- TMSP Trimethoxysilylpropanal
- the present inventors have surprisingly discovered that using a comparatively low concentration of aldehyde surface functionalization on mesoporous nanoparticles enables quick absorption of molecules into the pores of such particles, and, at the same time, enables controlled release of such materials from the pores over time.
- the conditions that are reported in the prior art is unsuitable for preparing quick absorbing, controlled release particles, because the higher concentration of surface aldehyde groups sterically interferes with the absorption and release processes.
- the low concentration of aldehyde surface functionalization disclosed herein is unsuitable for the purpose of covalently attaching secondary molecules (such as polymer, metallic particles, enzymes, and the like), because it is insufficient for supporting the desired concentration of secondary material.
- the instant nanoparticles are surface-functionalized with a plurality of aldehyde groups that are present in an amount of 0.1 to 1.0 mmol/g, 0.1 to 0.8 mmol/g, 0.1 to 0.7 mmol/g, 0.1 to 0.5 mmol/g, 0.1 to 0.4 mmol/g, or 0.1 to 0.3 mmol/g, such as about 0.1 mmol/g, about 0.2 mmol/g, about 0.3 mmol/g, about 0.4 mmol/g, about 0.5 mmol/g, about 0.6 mmol/g, about 0.7 mmol/g, about 0.8 mmol/g, about 0.9 mmol/g, or about 1.0 mmol/g.
- the instant surface aldehyde functionalized nanoparticles may be prepared using a "one pot" reaction procedure.
- a single reaction procedure may be used to accomplish both the synthesis of the nanoparticles and the inclusion of aldehyde groups on the surface of the nanoparticles.
- This single reaction procedure may be carried out by adding reagents to and appropriately manipulating the conditions within a single vessel.
- a one pot reaction procedure is characterized as involving a single chemical reaction.
- a multiple reaction procedure for producing surface functionalized nanoparticles which is characterized by the formation of nanoparticles in one reaction, and often in a first vessel, followed by the surface functionalization of the preformed nanoparticles in a second reaction, and often in a second vessel, or in the first vessel following formation and optional isolation of the nanoparticles.
- One-pot reaction procedures that may be used to form the instant nanoparticles are described more fully infra in connection with the presently disclosed methods for making aldehyde surface-functionalized mesoporous nanoparticles.
- the aldehyde groups are distributed substantially homogeneously (substantially evenly distributed) on the surface of a nanoparticle according to the present disclosure. It has been reported that when mesoporous materials are prepared in a procedure that is separate from the surface functionalization of the materials (i. e., are preformed prior to the functionalization reaction), the resulting product features functional groups that are comparatively non-homogeneously distributed on the surface of the mesoporous materials. See, e.g., Lim MH, et al, Chem. Mater. 1999, 11, 3285-3295. Therefore, one advantage of the presently disclosed one-pot reaction procedure is that there is substantially homogeneous distribution of the aldehyde groups on the surface of the nanoparticles.
- the pores of the nanoparticles may be loaded with one or more desired molecules, and the present nanoparticles are capable of absorbing a molecule having a molecular weight that is, for example, about 10 Da, about 20 Da, about 40 Da, about 50 Da, about 60 Da, about 70 Da, about 80 Da, about 90 Da, about 100 Da, about 200 Da, about 300 Da, about 400 Da, about 500 Da, about 600 Da, about 700 Da, about 800 Da, about 900 Da, about 1 kDa, about 1.1 kDa, about 1.2 kDa, about 1.3 kDa, about 1.4 kDa, about 1.5 kDa, about 1.6 kDa, about 1.7 kDa, about 1.8 kDa, about 1.9 kDa, about 2 kDa, about 2.1 kDa, about 2.2 kDa, about 2.3 kDa, about 2.4 kDa, about 2.5 kDa, about 2.6 kDa, about 2.7
- the nanoparticles may optionally be loaded with two or more different desired atomic or molecular moieties (see, for example, Figure 3 and 4 and corresponding examples).
- some of the pores of a given nanoparticle are loaded with a first molecule, while some of the other pores of the nanoparticle are loaded with a second, different molecule.
- a given pore of a nanoparticle is loaded with two or more different molecules. Procedures for loading the pores of the instant nanoparticles are described more fully infra.
- Moieties with which the pores of the instant nanoparticles may be loaded include any therapeutic or otherwise pharmaceutically active moiety that meets the presently disclosed size requirements.
- Exemplary "large molecules" that may be loaded into the pores of the present nanoparticles include nucleic acids, proteins, DNA, RNA, polysaccharides, enzymes, carbohydrates, and lipids.
- Classes of moieties with which the pores of the nanoparticles may be loaded include analgesics, antibiotics, antifungals, antiviral agents, antioxidants, antineoplastics, antiangiogenics, antithrombogenics, anti-inflammatories, steroids, cytokines, monoclonal antibodies, genetically modified biological molecules that have therapeutic effects, and growth factors.
- nanoparticles include vancomycin, rifampin, gentamycin, tobramycin, bupivacaine, mepivacaine, ibuprofen, insulin and its analogues, or any other desired drug agent.
- vancomycin vancomycin, rifampin, gentamycin, tobramycin, bupivacaine, mepivacaine, ibuprofen, insulin and its analogues, or any other desired drug agent.
- the inventive nanoparticles avoid the problem of initial burst release and are in fact capable of providing controlled release of moieties that has been loaded within at least some of the nanoparticle pores.
- the delivery of moieties may be characterized as substantially zero order release kinetics.
- the delivery of moieties at a controlled rate may be, for example, for about three days, about four days, about 5 days, about 6 days, about one week, about ten days, about two weeks, about three weeks, about four weeks, about five weeks, about six weeks, about seven weeks, about eight weights, about nine weeks, about ten weeks, about 11 weeks, or about 12 weeks.
- the delivery of the moieties over any such period of time may, in some embodiments, be characterized as substantially zero order release kinetics.
- the present disclosure also provides methods for making an aldehyde surface- functionalized mesoporous nanoparticle comprising synthesizing the nanoparticle using a one pot reaction procedure, wherein the procedure results in the nanoparticle being surface- functionalized with a plurality of aldehyde groups that are present in an amount of 0.1 to 1.0 mmol/g.
- the one pot reaction procedure may proceed in accordance with the following reaction: source of inorganic material
- Structure directing agents are used for the synthesis of mesoporous silica materials and are responsible for creating the mesostructure, i.e. , the mesopores.
- the structure directing agent may be, for example, a cationic surfactant, such as cetyltrimethylammonium bromide (C x TMABr), wherein "x" refers to the length of the carbon chain and can be, for example, 8 (Cg) to 18 (Cig); an anionic surfactant, such as cetyltrimethylammonium chloride (C X TMAC1) with length of carbon chain "x" being from 8 (Cg) to 18 (Cig); a nonionic surfactant, such as the poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) triblock copolymer family known as
- the structure directing agent may be combined with a solvent, such as an alcohol or water.
- the source of inorganic material may be a source of silica, calcium phosphate, hydroxyapatite, alumina, titanium dioxide, or any combination thereof.
- the source of such material may be, for example, tetraethyl orthosilicate (TEOS) or tetramethyl orthosilicate (TMOS).
- the source of aldehyde functional groups may be, for example, 2- (triethoxysilyl)butyraldehyde, 4-(triethoxysilyl)butyraldehyde, 3- (triethoxysilyl)propionaldehyde, or 4-(trimethoxysilyl)butyraldehyde.
- the source of inorganic material and the source of aldehyde functional groups are preferably added to the reaction medium with the structure directing agent already present.
- the source of inorganic material and the source of aldehyde functional groups may be added sequentially or at the same time.
- the reaction medium in which the present nanoparticles are formed is characterized by alkaline pH conditions.
- the pH of the reaction medium may be raised above neutral following introduction of the structure directing agent, following introduction of the source of inorganic material, or following introduction of the source of aldehyde functional groups. Any means for raising the pH above neutral may be used, for example, by adding sodium hydroxide.
- the alkaline pH conditions of the reaction medium may be about pH 10-13, about pH 12 being preferred.
- the reaction medium is optionally heated above room temperature.
- the solvent may be above room temperature, and thereafter the reaction medium may be maintained above room temperature.
- the temperature to which the reaction medium is raised and maintained may be about 30°C to about 100°C.
- the temperature may be, about 30°C, about 40°C, about 50°C, about 60°C, about 70°C, about 80°C, about 90°C, or about 100°C.
- the reaction medium may be maintained at the heated temperature for a desired period of time, for example, for about 30 min, about 45 min, about 1 hr, about 2 hr, about 3 hr, about 4 hr, about 5 hr, about 6 hr, about 8 hr, about 10 hr, or about 12 hr.
- the reaction medium will form a precipitate, and this precipitate may be separated, and optionally filtered, washed, and/or dried in order to yield the instant nanoparticles.
- the instant methods for making an aldehyde surface- functionalized mesoporous nanoparticle may comprise forming a solution of
- octadecyltrimethylammonium bromide in a solvent increasing the pH of the solution, adding a source of silica to the solution, adding a source of aldehyde functional groups to the solution, heating the solution for a time that is sufficient to form a precipitate, and drying the precipitate to obtain the nanoparticle.
- the solvent may be water.
- the pH of the solution may be raised to about pH 12.
- the source of silica may be tetraethyl orthosilicate.
- the source of aldehyde functional groups may be triethoxysilylbutyraldehyde.
- the source of silica and the source of aldehyde functional groups may be added to the solution separately or substantially
- the solution to which each of these ingredients has been added may be heated at about 80°C, and the time of heating may be about two hours.
- the resulting white precipitate may be dried for about 1-2 days in order to yield the instant nanoparticles.
- the nanoparticles may be subjected to a pore-expansion procedure in order to increase the proportion of mesopores in the nanoparticles.
- a pore-expansion procedure in order to increase the proportion of mesopores in the nanoparticles.
- the aldehyde-functionalized nanoparticles may be combined with a surfactant in order to form a suspension of the nanoparticles.
- the emulsion may then be heated, preferably under pressurized conditions. Following heating, the mixture may be filtered and washed in order to remove unreacted surfactant, and then dried in order to yield the pore-expanded nanoparticles.
- the pore expansion procedure may alternatively be carried out with respect to the instantly described microaggregates.
- the inventive microaggregates comprise a plurality of nanoparticles aggregated together, each of the nanoparticles comprising an inorganic material comprising a plurality of pores of which at least 90% have a diameter of 2 to 50 nm; and, the nanoparticles being surface- functionalized with a plurality of aldehyde groups that are present in an amount of 0.1 to 1.0 mmol/g.
- microaggregates may have any of the characteristics described above with respect to the inventive nanoparticles. Accordingly, the preceding description of the inventive nanoparticles is fully applicable to the nanoparticles that are aggregated in order to form the instant
- At least 90% of the pores of at least some of the nanoparticles of the instant aggregates may have a diameter of 2 to 40 microns, 2 to 30 microns, 2 to 25 microns, 2 to 20 microns, 2 to 10 microns, or 2 to 5 microns.
- the nanoparticles of the instant microaggregates may have at least one dimension that is 100-1000 nm, at least one dimension that is 100-500 nm, or at least one dimension that is 200-500 nm.
- the nanoparticles of the instant microaggregates may be surface-functionalized with a plurality of aldehyde groups that are present in an amount of 0.1 to 1.0 mmol/g, 0.1 to 0.8 mmol/g, 0.1 to 0.7 mmol/g, 0.1 to 0.5 mmol/g, 0.1 to 0.4 mmol/g, or 0.1 to 0.3 mmol/g.
- the nanoparticles of the instant microaggregates may have any of the characteristics described above with respect to the inventive nanoparticles, the nanoparticles of the instant microaggregates may also be prepared using a one-pot reaction procedure, for example, of the type described above.
- the instant microaggregates may comprise an aggregation of two or more different types of nanoparticles, each of which types of nanoparticles is consistent with any of the characteristics described above with respect to the presently disclosed nanoparticles.
- Respective "types" of nanoparticles may differ from one another, for example, in terms of one or more of dimensions, pore sizes, surface area, type of inorganic material, type of moiety loaded into the pores, and amount of aldehyde functional groups.
- a desired treatment and/or release profile can be optimized by permitting aggregation of different types of nanoparticles into a given microaggregate, and using the resulting microaggregate.
- the present disclosure is not meant to be limited to any particular theory of operation, it is believed that the nanoparticles form the instant microaggregates as a result of attractive van der Waals forces among the respective particles.
- the absence of electronegative hydroxyl groups on the nanoparticle surfaces and their replacement by less electronegative aldehyde groups could reduce the repulsive force between individual particles and facilitate aggregation.
- the present microaggregates comprise a plurality of aggregated nanoparticles.
- the number of nanoparticles in the aggregate is preferably sufficient to confer at least one micron-scale dimension on the resulting aggregate.
- the microaggregates may have at least one dimension that is from 0.5 microns to 15 microns, 0.5 microns to 12 microns, 1 micron to 15 microns, 1 micron to 12 microns, 1 micron to 10 microns, 2 microns to 12 microns, 2 microns to 10 microns, 3 microns to 12 microns, 4 microns to 12 microns, or 4 microns to 10 microns.
- the microaggregate has at least one dimension that is about 0.5 microns, about 1 micron, about 2 microns, about 3 microns, about 4 microns, about 5 microns, about 6 microns, about 7 microns, about 8 microns, about 9 microns, about 10 microns, about 11 microns about 12 microns, about 13 microns, about 14 microns, or about 15 microns.
- the number of nanoparticles in a microaggregate may be about 5, about 7, about 10, about 12, about 15, about 20, about 25, about 30, about 40, about 50, about 60, about 80, about 100, about 200, about 300, about 500, about 600, about 800, or about 1000.
- Also provided are methods for loading a microaggregate with a pharmaceutical agent comprising contacting a solution comprising the pharmaceutical agent with the
- microaggregate for a duration of time that is sufficient to load the microaggregate with the pharmaceutical agent, the microaggregate comprising a plurality of nanoparticles aggregated together, each of the nanoparticles comprising an inorganic material comprising a plurality of pores of which at least 90% have a diameter of 2 to 50 nm; and, the nanoparticles being surface- functionalized with a plurality of aldehyde groups that are present in an amount of 0.1 to 1.0 mmol/g, wherein less than about 30 minutes of the contacting are required in order to load at least some of the pores of the nanoparticles with the pharmaceutical agent.
- the present inventors have surprisingly discovered that using a low concentration of aldehyde surface functionalization on mesoporous nanoparticles enables quick absorption of molecules into the pores of such particles.
- the instantly described nanoparticles (which form the inventive
- microaggregates are capable of absorbing moieties into their pores after only about one hour or less of exposure to the moieties. For example, less than about 1 hour, less than about 45 minutes, less than about 30 minutes, less than about 25 minutes, less than about 20 minutes, less than about 15 minutes, or less than about 10 minutes of the contacting may be required in order to load at least some of the pores of the instant nanoparticles with a desired moiety. The described time periods are sufficient to load the instant nanoparticles with a pharmaceutically relevant quantity of the desired pharmaceutical moiety.
- the pharmaceutical agent may be any therapeutic or otherwise pharmaceutically active moiety that meets the size requirements previously described in connection with the inventive nanoparticles.
- the instant nanoparticles and microaggregates can be loaded with one pharmaceutical agent, or with two or more pharmaceutical agents. In other words, a single nanoparticle or a single microaggregate may be loaded with more than one pharmaceutical agent, as desired.
- the pharmaceutical agent is in a solution, mixture, suspension, slurry, or other like form when it is contacted with the microaggregate.
- the solution may contain the desired two or more pharmaceutical agents.
- the solution may comprise the pharmaceutical agent or agents and an appropriate solvent, carrier, excipient, or diluent.
- concentration of the pharmaceutical agent or agents may be optimized in accordance with the particular requirements for loading a therapeutically effective amount of pharmaceutical agent within the microaggregate, and can readily be determined by those of ordinary skill in the art.
- the step of contacting the solution with the microaggregate may be a simple matter of mixing the microaggregate into the solution comprising the pharmaceutical agent.
- the microaggregate may be suspended in a carrier substance, such as a liquid, such that the resulting suspension can be contacted with the solution comprising the
- the duration of the step of contacting the microaggregate with the solution containing the pharmaceutical agent may be considerably shorter than that which was required to load previous materials with a drug moiety.
- medical practitioners can prepare the drug-loaded microaggregates essentially
- the mixture may be at least partially dried.
- the mixture may be air-dried.
- the duration of the drying period may be, for example, about 5 minutes, about 7 minutes, about 10 minutes, about 12 minutes, about 15 minutes, about 20 minutes, about 25 minutes, or about 30 minutes. In other instances, it will not be necessary to dry the mixture at all, and the mixture can be used immediately after the contacting step.
- the present disclosure also relates to methods for administering a
- the respective characteristics of the microaggregate and the nanoparticles may be in accordance with any aspect of the preceding disclosure pertaining to the inventive microaggregages and nanoparticles, respectively.
- the step of contacting the subject with the microaggregate refers to creating physical contact between the subject and the microaggregate so that the pharmaceutical agent may, over time, release from the pores of the nanoparticles and effect the desired treatment.
- a site of treatment or a subject may be contacted with the microaggregate in any manner that is effective to yield this result.
- the microaggregate may be injected into the subject.
- the population including the microaggregate may be injected, poured, spread, or sprinkled on to the site of interest on or in the subject.
- the microaggregate may be incorporated into or attached to a medical device or other item that is in turn contacted with the subject.
- Examples include a bandage, a cast, an implant, or a prosthesis.
- the present application also pertains to an article comprising a nanoparticle that comprises an inorganic material comprising a plurality of pores of which at least 90% have a diameter of 2 to 50 nm, and, the nanoparticle being surface- functionalized with a plurality of aldehyde groups that are present in an amount of 0.1 to 1.0 mmol/g, or a microaggregate that comprises an aggregation of a plurality of such nanoparticles.
- the article may be, for example, a bandage, a cast, an implant, or a prosthesis.
- microaggregate or following the useful lifetime of the microaggregate (i.e., after the period of release of the pharmaceutical agent from the microaggrgegate has terminated).
- Example 1 Preparation of Nanoparticles and Microaggregates
- TEOS Tetra-ethylorthosilicate
- CigTAB octadecyltrimethylammoniumbromide
- sodium hydroxide pellets poly(ethyleneglycol)-bis-amine (PEG-amine) (molecular weight 3 kDa), dimethylhexadecylamine (DMH) and trimethyloctadecylammonium bromide, were obtained from Sigma Aldrich (St. Louis, MO), triethoxysilylbutyraldehyde was purchased from Gelest Inc (Philadelphia, PA) and used without further purification. Rifampin and vancomycin were separately obtained.
- TEOS Tetra-ethylorthosilicate
- CigTAB octadecyltrimethylammoniumbromide
- PEG-amine poly(ethyleneglycol)-bis-amine
- DH dimethylhexadecylamine
- MSMPs Aldehyde functionalized mesoporous silica microparticles
- Pore expansion was carried out by adding 1.6 g of dried aldehyde functionalized MSMPs or MSNs to an aqueous emulsion of 1.0 g of DMH in 60 ml of water and stirring for 20 min. The emulsion was transferred to a Teflon®-coated pressure vessel and kept in an oven at 110°C for 3 days. Then, the mixture was filtered and washed several times with deionized water to remove unreacted DMH. The samples were dried at 80°C for 1 day.
- the surfactants (CigTAB and DMH) were subsequently removed as follows. 0.8 g of pore-expanded MSNs or MSMPs were refluxed in 100 ml of methanol containing 1 ml of concentrated hydrochloric acid for 24 hr. Following refluxing, the sample was washed several times with ethanol, methanol, and water. The cleaned sample was then dried in an oven at 80°C for 2 days.
- FIG. 1 shows a scanning electron micrograph of the MSMPs.
- the average sizes of MSMPs were in the range of 4-10 ⁇ , and were formed through the aggregation of MSNs.
- Example 2 Loading of MSMPs with Pharmaceutical Moieties
- Dual loading of 20 mg vancomycin and 20 mg rifampin was carried out by dissolving the vancomycin and rifampin in separate 125 ⁇ aliquots of distilled water, and mixing the solution with 100 mg of MSMPs in a glass crucible with the help of a spatula, followed by air drying for 20 min before examining the release characteristics of the loaded MSMPs.
- the same amount of drug was dissolved in 250 ⁇ of distilled water and mixed with 100 mg of MSMPs using the same procedure as described above for dual loading.
- the loading period prior to the air drying step had a duration of less than 30 minutes.
- FIGS. 2A and 2B The individual mean cumulative vancomycin and rifampin release from MSMPs as a function of immersion time in PBS is shown in FIGS. 2A and 2B, respectively. It follows from the figures that the release of vancomycin and rifampin was time dependent. In both instances, drugs were released for about 3 weeks in controlled fashion.
- the initial vancomycin and rifampin release concentrations were 54.4 g/mL and 65.6 ⁇ g/mL. These amounts of vancomycin or rifampin released at day one were well above the minimum inhibitory concentration (MIC) values for MSSA and MRSA, and therefore suffice to prevent these microbes. In this context, it should be noted that MIC values are given at time zero, while thin sol-gel films continue to deliver drugs in a controlled manner over longer periods of time. The in vitro study demonstrated a long term, time dependent release of vancomycin or rifampin from MSMPs.
- FIG. 3 shows the simultaneous release of vancomycin and rifampin from the instant MSMPs. It shows that vancomycin and rifampin can be released simultaneously, in time dependent and controlled fashion. Initial release of both vancomycin (56.7 ⁇ g/mL) and rifampin (38.8 ⁇ g/mL) at day 1 exceeds the MIC level for individual drugs for both MSSA and MRSA. It was noted that the amount of release for both of the drugs could be increased by increasing the MSMPs dose.
- the drug-loaded MSMPs provided release of an amount of drug that was greater than the respective MICs (2 ⁇ for vancomycin and 0.5 ⁇ for rifampin against MRSA) for almost 3 weeks.
- the amount of drug release can be increased by increasing the dose of antibiotic loaded MSMPs.
- Antibiotic combination therapy as compared with monotherapy, can provide a broader spectrum of antibacterial effect, possible synergistic effects, and reduced risk for the emergence of resistance during therapy.
- the efficacy of combination therapy can be enhanced using a system that can deliver combinations of antibiotics locally and in controlled fashion. Such systems can minimize the adverse effects of combination therapies at are presently administered via intravenous injection or oral delivery.
- MRSA Staphylococcus aureus
- Aureus Rosenback ATTC strain 33591.
- Methicillin-resistant Staphylococcus aureus subspecies were purchased from ATTC (Manassas, VA). All experiments were run with at least three samples per data point, and measurements were made in triplicate.
- spectrophotometer Ultrospec Plus Spectrophotometer; Pharmacia LKB, Piscataway, NJ.
- the absorbance reading for a 0.5 McFarland standard (BD, Franklin Lakes, NJ) at 625nm was 0.08.
- a 0.5 McFarland standard corresponds to the same turbidity achieved of a bacterial solution of 10 s CFU/mL; therefore, the 10 s CFU/mL solution of S. aureus was created to match an absorbance of 0.08 ⁇ 10%.
- MSMPs without any drug loaded MSMPs loaded with 10% vancomycin
- MSMPs loaded with 10% vancomycin and 10% rifampin 15 mg of each sample was used, and each test was performed in triplicate.
- each sample was vortexed, and 2 mL of the solution was pipetted out and centrifuged @ 13000 rpm to precipitate the remaining bacteria in the solution.
- the bacterial pellet was separated from culture medium TSB and suspended in 1 mL PBS.
- FIG. 4A shows the bactericidal activity on day 1.
- MSMPs without any drug
- bacteria increased from 10 4 CFU/ml to 10 5 CFU/mL
- MSMPs with vancomycin alone were able to reduce the bacteria counts by 2.5 order of magnitude.
- MSMPs with dual antibiotics vancomycin and rifampin were able to completely kill the MRSA colonies.
- FIG. 4B shows the bactericidal effect of MSMPs, MSMPs with vancomycin and MSMPs with both vancomycin and rifampin after releasing drugs for 2, 3, and 4 days.
- the samples were challenged each day with fresh MRSA solution having concentration of 10 4 CFU/mL. It can be observed from these results that MSMPs with vancomycin alone and with vancomycin and rifampin retained their antimicrobial activity even at day 4. For MSMPs with vancomycin, there were two orders of magnitude reductions in bacteria growth, and with respect to MSMPs with dual antibiotics a complete inhibition of MRSA was observed even at day 4.
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PCT/US2016/032408 WO2016204896A1 (en) | 2015-06-16 | 2016-05-13 | Inorganic controlled release particles with fast drug loading |
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US20070116773A1 (en) * | 2005-07-05 | 2007-05-24 | Interuniversitair Microelektronica Centrum (Imec) | Metal nanostructures and pharmaceutical compositions |
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