EP3274459A4 - Platform for non-natural amino acid incorporation into proteins - Google Patents

Platform for non-natural amino acid incorporation into proteins Download PDF

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Publication number
EP3274459A4
EP3274459A4 EP16771096.1A EP16771096A EP3274459A4 EP 3274459 A4 EP3274459 A4 EP 3274459A4 EP 16771096 A EP16771096 A EP 16771096A EP 3274459 A4 EP3274459 A4 EP 3274459A4
Authority
EP
European Patent Office
Prior art keywords
proteins
platform
amino acid
natural amino
acid incorporation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP16771096.1A
Other languages
German (de)
French (fr)
Other versions
EP3274459A1 (en
Inventor
Sergey MUREEV
Zhenling CUI
Kirill Alexandrov
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Queensland UQ
Original Assignee
University of Queensland UQ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2015901121A external-priority patent/AU2015901121A0/en
Application filed by University of Queensland UQ filed Critical University of Queensland UQ
Publication of EP3274459A1 publication Critical patent/EP3274459A1/en
Publication of EP3274459A4 publication Critical patent/EP3274459A4/en
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/64—Cyclic peptides containing only normal peptide links
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/33—Chemical structure of the base
    • C12N2310/332—Abasic residue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/04—Other carbon-nitrogen ligases (6.3.4)
    • C12Y603/04019—Other carbon-nitrogen ligases (6.3.4) tRNA(Ile)-lysidine synthetase (6.3.4.19)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
EP16771096.1A 2015-03-27 2016-03-29 Platform for non-natural amino acid incorporation into proteins Withdrawn EP3274459A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AU2015901121A AU2015901121A0 (en) 2015-03-27 Platform for non-natural amino acid incorporation into peptides
PCT/AU2016/050239 WO2016154675A1 (en) 2015-03-27 2016-03-29 Platform for non-natural amino acid incorporation into proteins

Publications (2)

Publication Number Publication Date
EP3274459A1 EP3274459A1 (en) 2018-01-31
EP3274459A4 true EP3274459A4 (en) 2018-08-22

Family

ID=57003719

Family Applications (1)

Application Number Title Priority Date Filing Date
EP16771096.1A Withdrawn EP3274459A4 (en) 2015-03-27 2016-03-29 Platform for non-natural amino acid incorporation into proteins

Country Status (5)

Country Link
US (1) US20180171321A1 (en)
EP (1) EP3274459A4 (en)
JP (1) JP2018509172A (en)
CN (1) CN107614689A (en)
WO (1) WO2016154675A1 (en)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HUE054006T2 (en) 2011-12-28 2021-08-30 Chugai Pharmaceutical Co Ltd Peptide-compound cyclization method
CA2873793C (en) * 2012-05-18 2022-02-22 Medical Research Council Methods of incorporating an amino acid comprising a bcn group into a polypeptide using an orthogonal codon encoding it and an orthorgonal pylrs synthase
US10815489B2 (en) 2015-03-13 2020-10-27 Chugai Seiyaku Kabushiki Kaisha Modified aminoacyl-tRNA synthetase and use thereof
US11685942B2 (en) 2016-03-04 2023-06-27 President And Fellows Of Harvard College Methods for making polypeptides including d-amino acids
US12391971B2 (en) 2017-01-31 2025-08-19 Chugai Seiyaku Kabushiki Kaisha Method for synthesizing peptides in cell-free translation system
KR102744693B1 (en) 2017-06-09 2024-12-18 추가이 세이야쿠 가부시키가이샤 Method for synthesizing peptide containing n-substituted amino acid
KR102857378B1 (en) 2017-11-02 2025-09-11 유니버시티 오브 아이오와 리써치 파운데이션 A method for rescuing a stop codon through gene reassignment by ACE-tRNA
CN111479819B (en) 2017-12-15 2024-06-14 中外制药株式会社 Method for preparing peptide and method for treating base
EP3775216A4 (en) * 2018-04-03 2022-04-13 The University of Queensland OLIGONUCLEOTIDE-MEDIATED SENSE CODON REASSIGNMENT
WO2020040840A2 (en) 2018-06-01 2020-02-27 Northwestern University Expanding the chemical substrates for genetic code reprogramming
US20220002370A1 (en) 2018-09-27 2022-01-06 Xilio Development, Inc. Masked cytokine polypeptides
KR102841441B1 (en) 2018-11-07 2025-07-31 추가이 세이야쿠 가부시키가이샤 Method for preparing O-substituted serine derivatives
CN113056475B (en) 2018-11-30 2024-09-24 中外制药株式会社 Deprotection method for peptide compound or amide compound and resin removal method in solid phase reaction and method for producing peptide compound
CN119080856A (en) * 2018-12-26 2024-12-06 中外制药株式会社 Mutant tRNA for codon extension
JP7495420B2 (en) 2019-02-20 2024-06-04 ノヴォ ノルディスク アー／エス Aminoacyl-tRNA synthetases and uses thereof
JP7472101B2 (en) 2019-03-15 2024-04-22 中外製薬株式会社 Method for producing aromatic amino acid derivatives
CN111850020B (en) * 2019-04-25 2021-05-07 苏州鲲鹏生物技术有限公司 Introduction of Unnatural Amino Acids into Proteins Using a Plasmid System
BR112022007535A2 (en) 2019-11-07 2022-07-12 Chugai Pharmaceutical Co Ltd CYCLIC PEPTIDE COMPOUND HAVING KRAS INHIBITORY ACTION
CN115667539A (en) * 2020-06-25 2023-01-31 中外制药株式会社 Translation system with altered genetic code tables
TW202235431A (en) 2020-11-25 2022-09-16 美商艾希利歐發展股份有限公司 Tumor-specific cleavable linkers
JP2024510101A (en) * 2021-02-18 2024-03-06 チンファ ユニバーシティ protein translation system
WO2023282315A1 (en) * 2021-07-07 2023-01-12 味の素株式会社 Method for secretory production of unnatural-amino-acid-containing protein
WO2023044431A2 (en) * 2021-09-17 2023-03-23 Absci Corporation Composition of transfer rnas and use in production of proteins containing non-standard amino acids

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050136513A1 (en) * 2003-12-18 2005-06-23 The Scripps Research Institute Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1356036B1 (en) * 2001-01-25 2008-05-07 Anthony C. Forster Process and compositions for peptide, protein and peptidomimetic synthesis
EP1399574A2 (en) * 2001-05-17 2004-03-24 Aarhus University Improved vectors for gene therapy
WO2006135096A1 (en) * 2005-06-14 2006-12-21 Gifu University Method of site specifically introducing tyrosine analogue into protein
JP5798489B2 (en) * 2009-01-12 2015-10-21 ストロ バイオファーマ， インコーポレイテッド Monocharge system for selectively introducing unnatural amino acids into proteins using in vitro protein synthesis system
JP5725467B2 (en) * 2010-08-27 2015-05-27 国立大学法人 東京大学 New artificial translation synthesis system
JP6440055B2 (en) * 2013-05-10 2018-12-19 国立大学法人 東京大学 Method for producing peptide library, peptide library, and screening method
WO2014194129A2 (en) * 2013-05-29 2014-12-04 Brigham Young University Cell-free synthetic incorporation of non-natural amino acids into proteins

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050136513A1 (en) * 2003-12-18 2005-06-23 The Scripps Research Institute Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ANAËLLE DUMAS ET AL: "Designing logical codon reassignment - Expanding the chemistry in biology", CHEMICAL SCIENCE, vol. 6, no. 1, 1 January 2015 (2015-01-01), United Kingdom, pages 50 - 69, XP055490374, ISSN: 2041-6520, DOI: 10.1039/C4SC01534G *
CHIN J W: "Expanding and reprogramming the genetic code of cells and animals", ANNUAL REVIEW OF BIOCHEMISTRY, PALTO ALTO, CA, US, vol. 83, 1 June 2014 (2014-06-01), pages 379 - 408, XP002753743, ISSN: 0066-4154, [retrieved on 20140210], DOI: 10.1146/ANNUREV-BIOCHEM-060713-035737 *
KOWAL A K ET AL: "Twenty-first aminoacyl-tRNA synthetase-suppressor tRNA pairs for possible use in site-specific incorporation of amino acid analogues into proteins in eukaryotes and in eubacteria", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, US, vol. 98, no. 5, 27 February 2001 (2001-02-27), pages 2268 - 2273, XP002974767, ISSN: 0027-8424, DOI: 10.1073/PNAS.031488298 *
See also references of WO2016154675A1 *

Also Published As

Publication number Publication date
WO2016154675A1 (en) 2016-10-06
US20180171321A1 (en) 2018-06-21
JP2018509172A (en) 2018-04-05
CN107614689A (en) 2018-01-19
EP3274459A1 (en) 2018-01-31

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