EP3252456A3 - Fluorescent image analyzer and corresponding analyzing method - Google Patents

Fluorescent image analyzer and corresponding analyzing method Download PDF

Info

Publication number
EP3252456A3
EP3252456A3 EP17172419.8A EP17172419A EP3252456A3 EP 3252456 A3 EP3252456 A3 EP 3252456A3 EP 17172419 A EP17172419 A EP 17172419A EP 3252456 A3 EP3252456 A3 EP 3252456A3
Authority
EP
European Patent Office
Prior art keywords
sample
image analyzer
fluorescence image
fluorescent
pretreatment
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP17172419.8A
Other languages
German (de)
French (fr)
Other versions
EP3252456B1 (en
EP3252456A2 (en
Inventor
Kazuhiro Yamada
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sysmex Corp
Original Assignee
Sysmex Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP2017045666A external-priority patent/JP6231709B1/en
Application filed by Sysmex Corp filed Critical Sysmex Corp
Publication of EP3252456A2 publication Critical patent/EP3252456A2/en
Publication of EP3252456A3 publication Critical patent/EP3252456A3/en
Application granted granted Critical
Publication of EP3252456B1 publication Critical patent/EP3252456B1/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6419—Excitation at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Engineering & Computer Science (AREA)
  • Signal Processing (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

A fluorescence image analyzer, analyzing method, and pretreatment evaluation method capable of determining with high accuracy whether a sample is positive or negative are provided.
A pretreatment part 20 performs pretreatment including a step of labeling a target site with a fluorescent dye to prepare a sample 20a. A fluorescence image analyzer 10 measures and analyzes the sample 20a. The fluorescent image analyzer 10 includes light sources 121 to 124 to irradiate light on the sample 20a, imaging part 154 to capture the fluorescent light given off from the sample 20a irradiated by light, and processing part 11 for processing the fluorescence image captured by the imaging part 154. The processing part 11 extracts the bright spot of fluorescence generated from the fluorescent dye that labels the target site from the fluorescence image for each of a plurality of cells included in the sample 20a, and generates information used for determining whether the sample 20a is positive or negative based on the bright spots extracted for each of the plurality of cells.
EP17172419.8A 2016-05-31 2017-05-23 Fluorescent image analyzer and corresponding analyzing method Active EP3252456B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2016109005 2016-05-31
JP2017045666A JP6231709B1 (en) 2016-05-31 2017-03-10 Fluorescence image analyzer and analysis method

Publications (3)

Publication Number Publication Date
EP3252456A2 EP3252456A2 (en) 2017-12-06
EP3252456A3 true EP3252456A3 (en) 2018-03-28
EP3252456B1 EP3252456B1 (en) 2023-04-05

Family

ID=58772435

Family Applications (1)

Application Number Title Priority Date Filing Date
EP17172419.8A Active EP3252456B1 (en) 2016-05-31 2017-05-23 Fluorescent image analyzer and corresponding analyzing method

Country Status (1)

Country Link
EP (1) EP3252456B1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112166314A (en) 2018-05-30 2021-01-01 索尼公司 Fluorescence observation apparatus and fluorescence observation method
WO2020035068A1 (en) * 2018-08-17 2020-02-20 广州市锐博生物科技有限公司 Color base image generation method and system, and base recognition method and apparatus

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002093130A2 (en) * 2001-05-14 2002-11-21 Cancer Genetics, Inc. Methods of analyzing chromosomal translocations using fluorescence in situ hybridization (fish)
US20060073509A1 (en) * 1999-11-18 2006-04-06 Michael Kilpatrick Method for detecting and quantitating multiple subcellular components
EP1744145A2 (en) * 2005-07-12 2007-01-17 Sysmex Corporation Standard material for particle analyzer

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030104439A1 (en) 2001-11-30 2003-06-05 Finch Rosalynde J. Methods of identifying cellular target molecules

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060073509A1 (en) * 1999-11-18 2006-04-06 Michael Kilpatrick Method for detecting and quantitating multiple subcellular components
WO2002093130A2 (en) * 2001-05-14 2002-11-21 Cancer Genetics, Inc. Methods of analyzing chromosomal translocations using fluorescence in situ hybridization (fish)
EP1744145A2 (en) * 2005-07-12 2007-01-17 Sysmex Corporation Standard material for particle analyzer

Also Published As

Publication number Publication date
EP3252456B1 (en) 2023-04-05
EP3252456A2 (en) 2017-12-06

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