EP3210024A4 - Materials and methods for assaying living cells - Google Patents

Materials and methods for assaying living cells Download PDF

Info

Publication number
EP3210024A4
EP3210024A4 EP15852876.0A EP15852876A EP3210024A4 EP 3210024 A4 EP3210024 A4 EP 3210024A4 EP 15852876 A EP15852876 A EP 15852876A EP 3210024 A4 EP3210024 A4 EP 3210024A4
Authority
EP
European Patent Office
Prior art keywords
materials
methods
living cells
assaying
assaying living
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP15852876.0A
Other languages
German (de)
French (fr)
Other versions
EP3210024A1 (en
Inventor
Stuart Martin
Christopher M. Jewell
James I. ANDORKO
Elisabeth Lily SOOKLAL
Rebecca Whipple-Bettes
Kristi CHAKRABARTI
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Maryland at Baltimore
Original Assignee
University of Maryland at Baltimore
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2015/024207 external-priority patent/WO2015153948A2/en
Application filed by University of Maryland at Baltimore filed Critical University of Maryland at Baltimore
Publication of EP3210024A1 publication Critical patent/EP3210024A1/en
Publication of EP3210024A4 publication Critical patent/EP3210024A4/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/70—Mechanisms involved in disease identification
    • G01N2800/7023—(Hyper)proliferation
    • G01N2800/7028—Cancer
EP15852876.0A 2014-10-24 2015-10-07 Materials and methods for assaying living cells Pending EP3210024A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462068034P 2014-10-24 2014-10-24
PCT/US2015/024207 WO2015153948A2 (en) 2014-04-03 2015-04-03 Microtentacle imaging in patient tumor samples
PCT/US2015/054571 WO2016064584A1 (en) 2014-10-24 2015-10-07 Materials and methods for assaying living cells

Publications (2)

Publication Number Publication Date
EP3210024A1 EP3210024A1 (en) 2017-08-30
EP3210024A4 true EP3210024A4 (en) 2018-04-04

Family

ID=55761318

Family Applications (1)

Application Number Title Priority Date Filing Date
EP15852876.0A Pending EP3210024A4 (en) 2014-10-24 2015-10-07 Materials and methods for assaying living cells

Country Status (2)

Country Link
EP (1) EP3210024A4 (en)
WO (1) WO2016064584A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114903030B (en) * 2022-06-14 2024-03-01 安徽德维洛生物科技有限公司 Preservation method of active nucleated cells

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008144506A1 (en) * 2007-05-16 2008-11-27 University Of Maryland, Baltimore Apoptotic pathway targeting for the diagnosis and treatment of cancer
US20090137473A1 (en) * 2006-03-08 2009-05-28 Stuart Martin Inhibition of microtubule protrusion in cancer cells
US20140093953A1 (en) * 2012-10-01 2014-04-03 The Regents Of The University Of Michigan Non-adherent cell support and manufacturing method

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030157260A1 (en) 2001-10-25 2003-08-21 Rubner Michael F. Polyelectrolyte multilayers that influence cell growth, methods of applying them, and articles coated with them
US20140206559A1 (en) * 2011-05-23 2014-07-24 President And Fellows Of Harvard College Assay for metastatic potential of tumor cells
WO2015153948A2 (en) * 2014-04-03 2015-10-08 University Of Maryland, Baltimore Microtentacle imaging in patient tumor samples

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090137473A1 (en) * 2006-03-08 2009-05-28 Stuart Martin Inhibition of microtubule protrusion in cancer cells
WO2008144506A1 (en) * 2007-05-16 2008-11-27 University Of Maryland, Baltimore Apoptotic pathway targeting for the diagnosis and treatment of cancer
US20140093953A1 (en) * 2012-10-01 2014-04-03 The Regents Of The University Of Michigan Non-adherent cell support and manufacturing method

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
M A MATRONE ET AL: "Metastatic breast tumors express increased tau, which promotes microtentacle formation and the reattachment of detached breast tumor cells", ONCOGENE, vol. 29, no. 22, 15 March 2010 (2010-03-15), pages 3217 - 3227, XP055405818, ISSN: 0950-9232, DOI: 10.1038/onc.2010.68 *
M. A. MATRONE ET AL: "Microtentacles Tip the Balance of Cytoskeletal Forces in Circulating Tumor Cells", CANCER RESEARCH, vol. 70, no. 20, 5 October 2010 (2010-10-05), US, pages 7737 - 7741, XP055405496, ISSN: 0008-5472, DOI: 10.1158/0008-5472.CAN-10-1569 *
M. S. CHARPENTIER ET AL: "Curcumin Targets Breast Cancer Stem-like Cells with Microtentacles That Persist in Mammospheres and Promote Reattachment", CANCER RESEARCH, vol. 74, no. 4, 26 December 2013 (2013-12-26), US, pages 1250 - 1260, XP055405843, ISSN: 0008-5472, DOI: 10.1158/0008-5472.CAN-13-1778 *
MONICA CHARPENTIER ET AL: "Interplay of Stem Cell Characteristics, EMT, and Microtentacles in Circulating Breast Tumor Cells", CANCERS, vol. 5, no. 4, 1 November 2013 (2013-11-01), pages 1545 - 1565, XP055245305, DOI: 10.3390/cancers5041545 *
MONICA S CHARPENTIER ET AL: "Clinical Studies Co-Investigator UMGCC-0902: A Feasibility Study to Identify Microtentacles and Stem Cell Phenotype of Circulating Cancer Cells in Metastatic Breast Cancer Patients August 2010 - Present", BIOCHEMISTRY AND MOLECULAR BIOLOGY, 16 April 2014 (2014-04-16), pages 1 - 125, XP055274869, Retrieved from the Internet <URL:http://archive.hshsl.umaryland.edu/bitstream/10713/4081/8/Charpentier_umaryland_0373D_10501.pdf> [retrieved on 20160524] *
See also references of WO2016064584A1 *
SRIVATSAN KIDAMBI ET AL: "Controlling Primary Hepatocyte Adhesion and Spreading on Protein-Free Polyelectrolyte Multilayer Films", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 126, no. 50, 1 December 2004 (2004-12-01), US, pages 16286 - 16287, XP055274886, ISSN: 0002-7863, DOI: 10.1021/ja046188u *
WHIPPLE ET AL: "Detyrosinated microtubule protrusions in suspended mammary epithelial cells promote reattachment", EXPERIMENTAL CELL RESEARCH, ELSEVIER, AMSTERDAM, NL, vol. 313, no. 7, 3 April 2007 (2007-04-03), pages 1326 - 1336, XP022021032, ISSN: 0014-4827, DOI: 10.1016/J.YEXCR.2007.02.001 *

Also Published As

Publication number Publication date
WO2016064584A1 (en) 2016-04-28
EP3210024A1 (en) 2017-08-30

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