EP3021846A1 - Combinations comprising tricyclohexadecahexaene derivatives for use in the treatment of hepatitis c virus - Google Patents

Abstract

The present disclosure is generally directed to antiviral compounds, and more specifically directed to combinations of compounds which can inhibit the function of the NS5A protein encoded by Hepatitis C virus (HCV), compositions comprising such combinations, and methods for inhibiting the function of the NS5A protein.

Description

COMBINATIONS COMPRISING TRICYCLOHEXADECAHEXAENE DERIVATIVES FOR USE IN

THE TREATMENT OF HEPATITIS C VIRUS

CROSS-REFERENCE TO RELATED APPLICATIONS

This application claims the benefit of US Provisional Application Serial 5 Number 61/847,388, filed on July 17, 2013, which is hereby incorporated by

reference in its entirety.

The present disclosure is generally directed to antiviral compounds, and more specifically directed to combinations of compounds which can inhibit the function of the NS5A protein encoded by Hepatitis C virus (HCV), compositions comprising

10 such combinations, and methods for inhibiting the function of the NS5A protein.

HCV is a major human pathogen, infecting an estimated 170 million persons worldwide - roughly five times the number infected by human immunodeficiency virus type 1. A substantial fraction of these HCV infected individuals develop serious progressive liver disease, including cirrhosis and hepatocellular carcinoma.

15 Over the past decade the standard of care for the treatment of chronic HCV employed a combination of pegylated-interferon and ribavirin. The treatment has a non-optimal success rate in achieving sustained viral response (SVR) against the six major HCV genotypes, with a particularly low success rate against genotype 1, and causes numerous side effects. Recently approved drugs targeting the HCV NS3/4A

20 protease (Pis) (Victrelis ® and Incivek ®) are administered with pegylated-interferon and ribavirin and provide a major improvement in the percentage of patients who experience SVR and the treatment duration required to achieve SVR. However, there is a clear and urgent need to develop additional therapies to combat protease inhibitor resistance, to improve efficacy across all HCV genotypes, and to advance antiviral

25 therapy towards the ultimate goal of an interferon-free cure.

HCV is a positive-stranded RNA virus of approximately 9500 nucleotides in length and has a single open reading frame (ORF) encoding a single large polyprotein of about 3000 amino acids. In infected cells, this polyprotein is cleaved at multiple sites by cellular and viral proteases to produce the structural and non-structural (NS)

30 proteins. In the case of HCV, the generation of mature non-structural proteins (NS2, NS3, NS4A, NS4B, NS5A, and NS5B) is effected by two viral proteases. The first one is believed to be a metalloprotease and cleaves at the NS2-NS3 junction; the second one is a serine protease contained within the N-terminal region of NS3 (also referred to herein as NS3 protease) and mediates all the subsequent cleavages downstream of NS3, both in cis, at the NS3-NS4A cleavage site, and in trans, for the remaining NS4A-NS4B, NS4B-NS5A, NS5A-NS5B sites. The NS4A protein is a cofactor for the NS3 protease. The formation of a NS3-NS4A complex is necessary for proper protease activity. The NS3 protein also exhibits nucleoside triphosphatase and R A helicase activities. NS5A is a multi-functional protein required for viral R A replication and virion assembly. NS5B (also referred to herein as HCV polymerase) is a R A-dependent RNA polymerase that is responsible for viral RNA synthesis.

Considerable heterogeneity is found within the nucleotide and encoded amino acid sequence throughout the HCV genome due to the high error rate of the encoded RNA-dependent RNA polymerase which lacks a proof-reading capability. The clinical significance of the genetic heterogeneity of HCV is the propensity for mutations to arise during monotherapy treatment, thus combination therapies with HCV inhibitors that have pan-genotype coverage and act via independent

mechanisms are desired.

Compounds which selectively inhibit HCV viral replication and are useful for treating HCV-infected patients are desired. In particular, compounds which effectively inhibit the function of the NS5A protein are desired. The function and the essential role of NS5A protein for HCV replication are described, for example, in the following references: S. L. Tan, et al., Virology, 284:1-12 (2001); K. -J. Park, et al., J. Biol. Chem., 30711-30718 (2003); T. L. Tellinghuisen, et al, Nature, 435, 374 (2005); R. A. Love, et al, J. Virol, 83, 4395 (2009); N. Appel, et al., J. Biol. Chem., 281, 9833 (2006); L. Huang, J. Biol. Chem., 280, 36417 (2005); M. Gao, et al, Nature (2010); C. Rice, et al., WO2006093867.

A method has been described to identify compounds that demonstrate synergistic inhibition of HCV replicon activity when combined with the HCV NS5A inhibitor such as BMS-790052 (PCT/US2011/043785, filed July 13, 2011). In brief, each compound, when tested individually versus some NS5A resistant variants, is essentially inactive or much less active and only has synergistic inhibitory activity when tested in combination with an NS5A-targeting compound. The synergistic compounds were identified using titrations of test compounds in the presence of fixed concentrations of HCV NS5A inhibitors such as BMS-790052.

In a first aspect the present disclosure provides a combination comprising an NS5A-targeting compound and an NS5A synergist, which, when administered, provides synergistic anti-HCV activity against variants that contain mutation(s) conferring resistance to the NS5A-targeting compound alone, wherein the NS5A synergist is a compound of formula (I):

or a pharmaceutically acceptable salt thereof, wherein

R^1' and R^1' are independently selected from alkoxyalkyl, alkyl, cycloalkyl, and pyranyl, wherein the cycloalkyl and the pyranyl are optionally substituted with one, two, or three substituents independently selected from alkyl, halo, haloalkyl, hydroxy, and hydroxyalkyl; and

R² and R^2' are the same or different alkyl groups.

In a first embodiment of the first aspect the present disclosure provides a composition comprising said combination and one or more pharmaceutically acceptable carriers. In a second embodiment said composition comprises one or two additional compounds having anti-HCV activity. In a third embodiment at least one of the additional compounds is an interferon or a ribavirin. In a fourth embodiment the interferon is selected from interferon alpha 2B, pegylated interferon alpha, pegylated interferon lambda, consensus interferon, interferon alpha 2A, and lymphoblastoid interferon tau. In a fifth embodiment said composition comprises one or two additional compounds having anti-HCV activity wherein at least one of the additional compounds is effective to inhibit the function of a target selected from HCV protease, HCV polymerase, HCV helicase, HCV NS4B protein, HCV entry, HCV assembly, HCV egress, HCV NS5A protein, and IMPDH for the treatment of an HCV infection.

In a second aspect the present disclosure provides a method of treating an HCV infection in a patient, comprising administering to the patient a therapeutically effective amount of a combination of said combination, or a pharmaceutically acceptable salt thereof. In a first embodiment of the second aspect the method further comprises administering one or two additional compounds having anti-HCV activity prior to, after or simultaneously with the combination, or a pharmaceutically acceptable salt thereof. In a second embodiment at least one of the additional compounds is an interferon or a ribavirin. In a third embodiment the interferon is selected from interferon alpha 2B, pegylated interferon alpha, pegylated interferon lambda, consensus interferon, interferon alpha 2A, and lymphoblastoid interferon tau. In a fourth embodiment at least one of the additional compounds is effective to inhibit the function of a target selected from HCV protease, HCV polymerase, HCV helicase, HCV NS4B protein, HCV entry, HCV assembly, HCV egress, HCV NS5A protein, and IMPDH for the treatment of an HCV infection.

In another aspect the present disclosure provides a combination comprising an NS5A-targeting compound and an NS5A synergist, which, when administered, provides synergistic anti-HCV activity against variants that contain mutation(s) conferring resistance to the NS5A-targeting compound alone, wherein the NS5A synergist is a compound of formula (II):

cycloalkyl is optionally substituted with one, two, or three substituents independently selected from alkyl, halo, haloalkyl, hydroxy, and hydroxyalkyl.

Other aspects of the present disclosure may include suitable combinations of embodiments disclosed herein.

Yet other aspects and embodiments may be found in the description provided herein.

The description of the present disclosure herein should be construed in congruity with the laws and principals of chemical bonding. In some instances it may be necessary to remove a hydrogen atom in order to accommodate a substituent at any given location.

In some instances, the number of carbon atoms in any particular group is denoted before the recitation of the group. For example, the term "C₂_₆ alkenyl" denotes an alkenyl group containing two to six carbon atoms. Where these designations exist they supercede all other definitions contained herein.

It should be understood that the compounds encompassed by the present disclosure are those that are suitably stable for use as pharmaceutical agent.

It is intended that the definition of any substituent or variable at a particular location in a molecule be independent of its definitions elsewhere in that molecule.

All patents, patent applications, and literature references cited in the specification are herein incorporated by reference in their entirety. In the case of inconsistencies, the present disclosure, including definitions, will prevail.

As used in the present specification, the following terms have the meanings indicated:

As used herein, the singular forms "a", "an", and "the" include plural reference unless the context clearly dictates otherwise.

Unless stated otherwise, all aryl, cycloalkyl, and heterocyclyl groups of the present disclosure may be substituted as described in each of their respective definitions. For example, the aryl part of an arylalkyl group may be substituted as described in the definition of the term "aryl".

As used herein, the term "NS5A synergist" refers to a molecule that alone shows a weaker activity against HCV wild type than the NS5 A-targeting compound, but when combined with an NS5A-targeting compound shows a greater than three- fold increase in EC50 potency than the potency of the NS5A-targeting compound alone.

As used herein, the term "synergistic anti-HCV activity" refers to a greater than three-fold increase in EC50 potency than the potency of the NS5A-targeting compound alone.

As used herein, the term "NS5A-targeting compound", refers to a molecule that inhibits HCV replication for which at least one resistance substitution maps to the NS5A protein and most commonly within, but not limited to, the first 100 residues of NS5A. The term "alkoxy," as used herein, refers to an alkyl group attached to the parent molecular group through an oxygen atom.

The term "alkoxyalkyl," as used herein, refers to an alkyl group substituted with one, two, or three alkoxy groups.

The term "alkyl," as used herein, refers to a group derived from a straight or branched chain saturated hydrocarbon containing from one to seven carbon atoms.

The term "cycloalkyl," as used herein, refers to a three- to seven-membered monocyclic saturated carbocyclic ring.

The term "halo," as used herein, refers to CI, Br, F, or I.

The term "haloalkyl," as used herein, refers to an alkyl group substituted with one, two, three, or four halogen atoms.

The term "hydroxy," as used herein, refers to -OH.

The term "hydroxyalkyl," as used herein, refers to an alkyl group substituted with one, two, or three hydroxy groups.

Asymmetric centers exist in the compounds of the present disclosure. These centers are designated by the symbols "R" or "S", depending on the configuration of substituents around the chiral carbon atom. It should be understood that the disclosure encompasses all stereochemical isomeric forms, or mixtures thereof, which possess the ability to inhibit NS5A. Individual stereoisomers of compounds can be prepared synthetically from commercially available starting materials which contain chiral centers or by preparation of mixtures of enantiomeric products followed by separation such as conversion to a mixture of diastereomers followed by separation or recrystallization, chromatographic techniques, or direct separation of enantiomers on chiral chromatographic columns. Starting compounds of particular stereochemistry are either commercially available or can be made and resolved by techniques known in the art.

Certain compounds of the present disclosure may also exist in different stable conformational forms which may be separable. Torsional asymmetry due to restricted rotation about an asymmetric single bond, for example because of steric hindrance or ring strain, may permit separation of different conformers. The present disclosure includes each conformational isomer of these compounds and mixtures thereof. The compounds of the present disclosure also exist as tautomers; therefore the present disclosure also encompasses all tautomeric forms.

The term "compounds of the present disclosure", and equivalent expressions, are meant to embrace the compounds making up the combination of the present disclosure and pharmaceutically acceptable enantiomers, diastereomers, and salts thereof. Similarly, references to intermediates are meant to embrace their salts where the context so permits.

The present disclosure is intended to include all isotopes of atoms occurring in the present compounds. Isotopes include those atoms having the same atomic number but different mass numbers. By way of general example and without limitation, isotopes of hydrogen include deuterium and tritium. Isotopes of carbon include ¹³C and ¹⁴C. Isotopically-labeled compounds of the invention can generally be prepared by conventional techniques known to those skilled in the art or by processes analogous to those described herein, using an appropriate isotopically- labeled reagent in place of the non-labeled reagent otherwise employed. Such compounds may have a variety of potential uses, for example as standards and reagents in determining biological activity. In the case of stable isotopes, such compounds may have the potential to favorably modify biological, pharmacological, or pharmacokinetic properties.

The compounds of the present disclosure can exist as pharmaceutically acceptable salts. The term "pharmaceutically acceptable salt," as used herein, represents salts or zwitterionic forms of the compounds of the present disclosure which are water or oil-soluble or dispersible, which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of patients without excessive toxicity, irritation, allergic response, or other problem or complication commensurate with a reasonable benefit/risk ratio, and are effective for their intended use. The salts can be prepared during the final isolation and purification of the compounds or separately by reacting a suitable nitrogen atom with a suitable acid. Representative acid addition salts include acetate, adipate, alginate, citrate, aspartate, benzoate, benzenesulfonate, bisulfate, butyrate, camphorate, camphorsulfonate; digluconate, glycerophosphate, hemisulfate, heptanoate, hexanoate, formate, fumarate, hydrochloride, hydrobromide, hydroiodide, 2-hydroxyethanesulfonate, lactate, maleate, mesitylenesulfonate, methanesulfonate, naphthylenesulfonate, nicotinate, 2-naphthalenesulfonate, oxalate, palmoate, pectinate, persulfate, 3- phenylproprionate, picrate, pivalate, propionate, succinate, tartrate, trichloroacetate, trifluoroacetate, phosphate, glutamate, bicarbonate, para-toluenesulfonate, and undecanoate. Examples of acids which can be employed to form pharmaceutically acceptable addition salts include inorganic acids such as hydrochloric, hydrobromic, sulfuric, and phosphoric, and organic acids such as oxalic, maleic, succinic, and citric.

Basic addition salts can be prepared during the final isolation and purification of the compounds by reacting a carboxy group with a suitable base such as the hydroxide, carbonate, or bicarbonate of a metal cation or with ammonia or an organic primary, secondary, or tertiary amine. The cations of pharmaceutically acceptable salts include lithium, sodium, potassium, calcium, magnesium, and aluminum, as well as nontoxic amine cations such as ammonium, tetramethylammonium, tetraethylammonium, methylamine, dimethylamine, trimethylamine, triethylamine, diethylamine, ethylamine, tributylamine, pyridine, Ν,Ν-dimethylaniline, N- methylpiperidine, N-methylmorpholine, dicyclohexylamine, procaine, dibenzylamine, Ν,Ν-dibenzylphenethylamine, and Ν,Ν'-dibenzylethylenediamine. Other

representative organic amines useful for the formation of base addition salts include ethylenediamine, ethanolamine, diethanolamine, piperidine, and piperazine.

When it is possible that, for use in therapy, therapeutically effective amounts of each compound of the combination, as well as pharmaceutically acceptable salts thereof, may be administered as the raw chemical, it is possible to present the active ingredient as a pharmaceutical composition. Accordingly, the disclosure further provides pharmaceutical compositions, which include therapeutically effective amounts of the compounds comprising the combination or pharmaceutically acceptable salts thereof, and one or more pharmaceutically acceptable carriers, diluents, or excipients. The term "therapeutically effective amount," as used herein, refers to the total amount of each active component that is sufficient to show a meaningful patient benefit, e.g., a sustained reduction in viral load. When applied to an individual active ingredient, administered alone, the term refers to that ingredient alone. When applied to a combination, the term refers to combined amounts of the active ingredients that result in the therapeutic effect, whether administered in combination, serially, or simultaneously. The compounds of the combination and pharmaceutically acceptable salts thereof, are as described above. The carrier(s), diluent(s), or excipient(s) must be acceptable in the sense of being compatible with the other ingredients of the formulation and not deleterious to the recipient thereof. In accordance with another aspect of the present disclosure there is also provided a process for the preparation of a pharmaceutical formulation including admixing the compounds of the combination, or pharmaceutically acceptable salts thereof, with one or more pharmaceutically acceptable carriers, diluents, or excipients. The term "pharmaceutically acceptable," as used herein, refers to those compounds, materials, compositions, and/or dosage forms which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of patients without excessive toxicity, irritation, allergic response, or other problem or complication commensurate with a reasonable benefit/risk ratio, and are effective for their intended use.

Pharmaceutical formulations may be presented in unit dose forms containing a predetermined amount of active ingredient per unit dose. Dosage levels of between about 0.01 and about 250 milligram per kilogram ("mg/kg") body weight per day, preferably between about 0.05 and about 100 mg/kg body weight per day of the compounds of the present disclosure are typical in a monotherapy for the prevention and treatment of HCV mediated disease. Typically, the pharmaceutical compositions of this disclosure will be administered from about 1 to about 5 times per day or alternatively, as a continuous infusion. Such administration can be used as a chronic or acute therapy. The amount of active ingredient that may be combined with the carrier materials to produce a single dosage form will vary depending on the condition being treated, the severity of the condition, the time of administration, the route of administration, the rate of excretion of the compound employed, the duration of treatment, and the age, gender, weight, and condition of the patient. Preferred unit dosage formulations are those containing a daily dose or sub-dose, as herein above recited, or an appropriate fraction thereof, of an active ingredient. Generally, treatment is initiated with small dosages substantially less than the optimum dose of the compound. Thereafter, the dosage is increased by small increments until the optimum effect under the circumstances is reached. In general, the compound is most desirably administered at a concentration level that will generally afford antivirally effective results without causing any harmful or deleterious side effects.

When the compositions of this disclosure comprise a combination of a compound of the present disclosure and one or more additional therapeutic or prophylactic agent, both the compound and the additional agent are usually present at dosage levels of between about 10 to 150%, and more preferably between about 10 and 80% of the dosage normally administered in a monotherapy regimen.

Pharmaceutical formulations may be adapted for administration by any appropriate route, for example by the oral (including buccal or sublingual), rectal, nasal, topical (including buccal, sublingual, or transdermal), vaginal, or parenteral (including subcutaneous, intracutaneous, intramuscular, intra-articular, intrasynovial, intrasternal, intrathecal, intralesional, intravenous, or intradermal injections or infusions) route. Such formulations may be prepared by any method known in the art of pharmacy, for example by bringing into association the active ingredient with the carrier(s) or excipient(s). Oral administration or administration by injection are preferred.

Pharmaceutical formulations adapted for oral administration may be presented as discrete units such as capsules or tablets; powders or granules; solutions or suspensions in aqueous or non-aqueous liquids; edible foams or whips; or oil-in- water liquid emulsions or water-in-oil emulsions.

For instance, for oral administration in the form of a tablet or capsule, the active drug component can be combined with an oral, non-toxic pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like. Powders are prepared by comminuting the compound to a suitable fine size and mixing with a similarly comminuted pharmaceutical carrier such as an edible carbohydrate, as, for example, starch or mannitol. Flavoring, preservative, dispersing, and coloring agent can also be present.

Capsules are made by preparing a powder mixture, as described above, and filling formed gelatin sheaths. Glidants and lubricants such as colloidal silica, talc, magnesium stearate, calcium stearate, or solid polyethylene glycol can be added to the powder mixture before the filling operation. A disintegrating or solubilizing agent such as agar-agar, calcium carbonate, or sodium carbonate can also be added to improve the availability of the medicament when the capsule is ingested.

Moreover, when desired or necessary, suitable binders, lubricants,

disintegrating agents, and coloring agents can also be incorporated into the mixture. Suitable binders include starch, gelatin, natural sugars such as glucose or beta-lactose, corn sweeteners, natural and synthetic gums such as acacia, tragacanth or sodium alginate, carboxymethylcellulose, polyethylene glycol, and the like. Lubricants used in these dosage forms include sodium oleate, sodium chloride, and the like.

Disintegrators include, without limitation, starch, methyl cellulose, agar, betonite, xanthan gum, and the like. Tablets are formulated, for example, by preparing a powder mixture, granulating or slugging, adding a lubricant and disintegrant, and pressing into tablets. A powder mixture is prepared by mixing the compound, suitable comminuted, with a diluent or base as described above, and optionally, with a binder such as carboxymethylcellulose, an aliginate, gelating, or polyvinyl pyrrolidone, a solution retardant such as paraffin, a resorption accelerator such as a quaternary salt and/or and absorption agent such as betonite, kaolin, or dicalcium phosphate. The powder mixture can be granulated by wetting with a binder such as syrup, starch paste, acadia mucilage, or solutions of cellulosic or polymeric materials and forcing through a screen. As an alternative to granulating, the powder mixture can be run through the tablet machine and the result is imperfectly formed slugs broken into granules. The granules can be lubricated to prevent sticking to the tablet forming dies by means of the addition of stearic acid, a stearate salt, talc, or mineral oil. The lubricated mixture is then compressed into tablets. The compounds of the present disclosure can also be combined with a free flowing inert carrier and compressed into tablets directly without going through the granulating or slugging steps. A clear or opaque protective coating consisting of a sealing coat of shellac, a coating of sugar or polymeric material, and a polish coating of wax can be provided. Dyestuffs can be added to these coatings to distinguish different unit dosages.

Oral fluids such as solution, syrups, and elixirs can be prepared in dosage unit form so that a given quantity contains a predetermined amount of the compound. Syrups can be prepared by dissolving the compound in a suitably flavored aqueous solution, while elixirs are prepared through the use of a non-toxic vehicle.

Solubilizers and emulsifiers such as ethoxylated isostearyl alcohols and

polyoxyethylene sorbitol ethers, preservatives, fiavor additive such as peppermint oil or natural sweeteners, or saccharin or other artificial sweeteners, and the like can also be added.

Where appropriate, dosage unit formulations for oral administration can be microencapsulated. The formulation can also be prepared to prolong or sustain the release as for example by coating or embedding particulate material in polymers, wax, or the like.

The compounds of Formula (I), and pharmaceutically acceptable salts thereof, can also be administered in the form of liposome delivery systems, such as small unilamellar vesicles, large unilamellar vesicles, and multilamellar vesicles.

Liposomes can be formed from a variety of phopholipids, such as cholesterol, stearylamine, or phophatidylcholines.

The compounds of the combination and pharmaceutically acceptable salts thereof may also be delivered by the use of monoclonal antibodies as individual carriers to which the compound molecules are coupled. The compounds may also be coupled with soluble polymers as targetable drug carriers. Such polymers can include polyvinylpyrrolidone, pyran copolymer,

polyhydroxypropylmethacrylamidephenol, polyhydroxyethylaspartamidephenol, or polyethyleneoxidepolylysine substituted with palitoyl residues. Furthermore, the compounds may be coupled to a class of biodegradable polymers useful in achieving controlled release of a drug, for example, polylactic acid, polepsilon caprolactone, polyhydroxy butyric acid, polyorthoesters, polyacetals, polydihydropyrans, polycyanoacrylates, and cross-linked or amphipathic block copolymers of hydrogels.

Pharmaceutical formulations adapted for transdermal administration may be presented as discrete patches intended to remain in intimate contact with the epidermis of the recipient for a prolonged period of time. For example, the active ingredient may be delivered from the patch by iontophoresis as generally described in Pharmaceutical Research 1986, 5(6), 318.

Pharmaceutical formulations adapted for topical administration may be formulated as ointments, creams, suspensions, lotions, powders, solutions, pastes, gels, sprays, aerosols, or oils.

For treatments of the eye or other external tissues, for example mouth and skin, the formulations are preferably applied as a topical ointment or cream. When formulated in an ointment, the active ingredient may be employed with either a paraffinic or a water-miscible ointment base. Alternatively, the active ingredient may be formulated in a cream with an oil-in-water cream base or a water-in oil base.

Pharmaceutical formulations adapted for topical administrations to the eye include eye drops wherein the active ingredient is dissolved or suspended in a suitable carrier, especially an aqueous solvent. Pharmaceutical formulations adapted for topical administration in the mouth include lozenges, pastilles, and mouth washes.

Pharmaceutical formulations adapted for rectal administration may be presented as suppositories or as enemas.

Pharmaceutical formulations adapted for nasal administration wherein the carrier is a solid include a course powder having a particle size for example in the range 20 to 500 microns which is administered in the manner in which snuff is taken, i.e., by rapid inhalation through the nasal passage from a container of the powder held close up to the nose. Suitable formulations wherein the carrier is a liquid, for administration as a nasal spray or nasal drops, include aqueous or oil solutions of the active ingredient.

Pharmaceutical formulations adapted for administration by inhalation include fine particle dusts or mists, which may be generated by means of various types of metered, dose pressurized aerosols, nebulizers, or insufflators.

Pharmaceutical formulations adapted for vaginal administration may be presented as pessaries, tampons, creams, gels, pastes, foams, or spray formulations.

Pharmaceutical formulations adapted for parenteral administration include aqueous and non-aqueous sterile injection solutions which may contain anti-oxidants, buffers, bacteriostats, and soutes which render the formulation isotonic with the blood of the intended recipient; and aqueous and non-aqueous sterile suspensions which may include suspending agents and thickening agents. The formulations may be presented in unit-dose or multi-dose containers, for example sealed ampoules and vials, and may be stored in a freeze-dried (lyophilized) condition requiring only the addition of the sterile liquid carrier, for example water for injections, immediately prior to use. Extemporaneous injection solutions and suspensions may be prepared from sterile powders, granules, and tablets.

It should be understood that in addition to the ingredients particularly mentioned above, the formulations may include other agents conventional in the art having regard to the type of formulation in question, for example those suitable for oral administration may include flavoring agents.

The term "patient" includes both human and other mammals.

The term "treating" refers to: (i) preventing a disease, disorder or condition from occurring in a patient that may be predisposed to the disease, disorder, and/or condition but has not yet been diagnosed as having it; (ii) inhibiting the disease, disorder, or condition, i.e., arresting its development; and (iii) relieving the disease, disorder, or condition, i.e., causing regression of the disease, disorder, and/or condition.

The compounds of the present disclosure can also be administered with a cyclosporin, for example, cyclosporin A. Cyclosporin A has been shown to be active against HCV in clinical trials (Hepatology 2003, 38, 1282; Biochem. Biophys. Res. Commun. 2004, 313, 42; J. Gastroenterol. 2003, 38, 567).

Table A below lists some illustrative examples of compounds that can be administered with the compounds of this disclosure. The compounds of the disclosure can be administered with other anti-HCV activity compounds in combination therapy, either jointly or separately, or by combining the compounds into a composition.

Table A

Type of Inhibitor

Brand Name Physiological Class Source Company or Target

Endo

Pharmaceuticals

Summetrel Antiviral antiviral

Holdings Inc., Chadds Ford, PA

GS-9132 (ACH-

Antiviral HCV Inhibitor Achillion / Gilead 806)

Pyrazolopyrimidine

compounds and

salts Arrow

Antiviral HCV Inhibitors

From WO- Therapeutics Ltd. 2005047288

26 May 2005

Ribapharm Inc.,

Levovirin Antiviral IMPDH inhibitor

Costa Mesa, CA

Vertex

Merimepodib Pharmaceuticals

Antiviral IMPDH inhibitor (VX-497) Inc., Cambridge,

MA

XTL

XTL-6865 (XTL- monoclonal Biopharmaceutica

Antiviral

002) antibody Is Ltd., Rehovot,

Isreal

Vertex

Pharmaceuticals

Telaprevir

NS3 serine Inc., Cambridge, (VX-950, LY- Antiviral

protease inhibitor MA/ Eli Lilly and 570310)

Co. Inc., Indianapolis, IN

NS5B Replicase Wyeth /

HCV-796 Antiviral

Inhibitor Viropharma

NS5B Replicase

NM-283 Antiviral Idenix / Novartis

Inhibitor Type of Inhibitor

Brand Name Physiological Class Source Company or Target

NS5B Replicase Gene Labs /

GL-59728 Antiviral

Inhibitor Novartis

NS5B Replicase Gene Labs /

GL-60667 Antiviral

Inhibitor Novartis

NS5B Replicase

2'C MeA Antiviral Gilead

Inhibitor

NS5B Replicase

PSI 6130 Antiviral Roche

Inhibitor

NS5B Replicase

R1626 Antiviral Roche

Inhibitor

2'C Methyl NS5B Replicase

Antiviral Merck adenosine Inhibitor

Japan Tobacco

JTK-003 Antiviral RdRp inhibitor

Inc., Tokyo, Japan

ICN

Levovirin Antiviral ribavirin Pharmaceuticals,

Costa Mesa, CA

Schering-Plough

Ribavirin Antiviral ribavirin Corporation,

Kenilworth, NJ

Ribapharm Inc.,

Viramidine Antiviral Ribavirin Prodrug

Costa Mesa, CA

Ribozyme

Heptazyme Antiviral ribozyme Pharmaceuticals

Inc., Boulder, CO

Boehringer serine protease Ingelheim Pharma

BILN-2061 Antiviral

inhibitor KG, Ingelheim,

Germany serine protease

SCH 503034 Antiviral Schering Plough inhibitor Type of Inhibitor

Brand Name Physiological Class Source Company or Target

SciClone

Immune Pharmaceuticals

Zadazim Immune modulator

modulator Inc., San Mateo,

CA

Maxim immune Pharmaceuticals

Ceplene Immunomodulator

modulator Inc., San Diego,

CA

F. Hoffmann-La

HCV IgG

Roche LTD,

CellCept Immunosuppressant immunoBasel, suppressant

Switzerland

Nabi

HCV IgG

Biopharmaceutica

Civacir Immunosuppressant immunoIs Inc., Boca suppressant

Raton, FL

Human Genome

Albuferon - a Interferon albumin IFN-a2b Sciences Inc.,

Rockville, MD

InterMune

IFN

Infergen A Interferon Pharmaceuticals alfacon- 1

Inc., Brisbane, CA

Intarcia

Omega IFN Interferon IFN-ω

Therapeutics

Transition

IFN-β and

IFN- and EMZ701 Interferon Therapeutics Inc.,

EMZ701

Ontario, Canada

Serono, Geneva,

Rebif Interferon TFN^la

Switzerland

F. Hoffmann-La Roche LTD,

Roferon A Interferon IFN-a2a

Basel,

Switzerland Type of Inhibitor

Brand Name Physiological Class Source Company or Target

Schering-Plough

Intron A Interferon IFN-a2b Corporation,

Kenilworth, NJ

RegeneRx Biopharma. Inc., Bethesda, MD/

Intron A and IFN-a2b/al-

Interferon SciClone Zadaxin thymosin

Pharmaceuticals Inc, San Mateo, CA

Schering-Plough

Rebetron Interferon IFN-a2b/ribavirin Corporation,

Kenilworth, NJ

InterMune Inc.,

Actimmune Interferon INF-γ

Brisbane, CA

Interferon- Interferon Interferon- -la Serono

Viragen/

Multiferon Interferon Long lasting IFN

Valentis

Lympho-blastoid Glaxo SmithKline

Wellferon Interferon

IFN-anl pic, Uxbridge, UK

Viragen Inc.,

Omniferon Interferon natural IFN-a

Plantation, FL

F. Hoffmann-La

PEGylated IFN- Roche LTD,

Pegasys Interferon

a2a Basel,

Switzerland

PEGylated IFN- Maxim

Pegasys and a2a/ Pharmaceuticals

Interferon

Ceplene immune Inc., San Diego, modulator CA Type of Inhibitor

Brand Name Physiological Class Source Company or Target

F. Hoffmann-La

Pegasys and PEGylated IFN- Roche LTD,

Interferon

Ribavirin a2a/ribavirin Basel,

Switzerland

Schering-Plough

PEGylated IFN-

PEG-Intron Interferon Corporation, a2b

Kenilworth, NJ

Schering-Plough

PEG-Intron / PEGylated IFN-

Interferon Corporation, Ribavirin a2b/ribavirin

Kenilworth, NJ

Indevus

Pharmaceuticals

IP-501 Liver protection antifibrotic

Inc., Lexington, MA

Idun

Pharmaceuticals

IDN-6556 Liver protection caspase inhibitor

Inc., San Diego, CA

InterMune serine protease

ITMN-191 (R-7227) Antiviral Pharmaceuticals inhibitor

Inc., Brisbane, CA

NS5B Replicase

GL-59728 Antiviral Genelabs

Inhibitor

ANA-971 Antiviral TLR-7 agonist Anadys

serine protease

Boceprevir Antiviral Schering Plough inhibitor

Tibotec BVBA, serine protease

TMS-435 Antiviral Mechelen,

inhibitor

Belgium

Boehringer serine protease Ingelheim Pharma

BI-201335 Antiviral

inhibitor KG, Ingelheim,

Germany Type of Inhibitor

Brand Name Physiological Class Source Company or Target

serine protease

MK-7009 Antiviral Merck

inhibitor

PF-00868554 Antiviral replicase inhibitor Pfizer

Anadys

Non-Nucleoside

Pharmaceuticals,

ANA598 Antiviral NS5B Polymerase

Inc., San Diego, Inhibitor

CA, USA

Idenix

Non-Nucleoside

Pharmaceuticals,

IDX375 Antiviral Replicase

Cambridge, MA,

Inhibitor

USA

Boehringer

NS5B Polymerase Ingelheim Canada

BILB 1941 Antiviral

Inhibitor Ltd R&D, Laval,

QC, Canada

Nucleoside

PSI-7851 Antiviral Polymerase Gilead

Inhibitor

Nucleotide NS5B

sofosbuvir Antiviral Polymerase Gilead

Inhibitor

Antiviral NS5B Polymerase ViroChem

VCH-759

Inhibitor Pharma

Antiviral NS5B Polymerase ViroChem

VCH-916

Inhibitor Pharma

Antiviral NS5B Polymerase

GS-9190 Gilead

Inhibitor

Peg-interferon Antiviral ZymoGenetics/Bri

Interferon

lamda stol-Myers Squibb

Antiviral Bristol-Myers daclatasvir NS5A inhibitor

Squibb

Antiviral NS5B Polymerase Bristol-Myers

BMS-791325

Inhibitor Squibb Type of Inhibitor

Brand Name Physiological Class Source Company or Target

ACH-3102 Antiviral

NS5A inhibitor Achillion

Antiviral serine protease Bristol-Myers asunaprevir

inhibitor Squibb

IDX-719 Antiviral NS5A inhibitor Idenix

Ledipasvir Antiviral NS5A inhibitor Gilead

GS-5816 Antiviral NS5A inhibitor Gilead

Ombitasvir Antiviral NS5A inhibitor Abbvie

GSK-2336805 Antiviral NS5A inhibitor Glaxo SmithKline

PPI-461 Antiviral NS5A inhibitor Presidio

EDP-239 Antiviral NS5A inhibitor Enanta

Elbasvir Antiviral NS5A inhibitor Merck

Antiviral Nucleotide

IDX-21437 Polymerase Idenix

Inhibitor

Samatasvir Antiviral NS5A Inhibitor Idenix

The compounds of the present disclosure may also be used as laboratory reagents. Compounds may be instrumental in providing research tools for designing of viral replication assays, validation of animal assay systems and structural biology studies to further enhance knowledge of the HCV disease mechanisms. Further, the compounds of the present disclosure are useful in establishing or determining the binding site of other antiviral compounds, for example, by competitive inhibition.

The compounds of this disclosure may also be used to treat or prevent viral contamination of materials and therefore reduce the risk of viral infection of laboratory or medical personnel or patients who come in contact with such materials, e.g., blood, tissue, surgical instruments and garments, laboratory instruments and garments, and blood collection or transfusion apparatuses and materials.

This disclosure is intended to encompass compounds having Formula (I) when prepared by synthetic processes or by metabolic processes including those occurring in the human or animal body (in vivo) or processes occurring in vitro.

The abbreviations used in the present application, including particularly in the illustrative schemes and examples which follow, are well-known to those skilled in the art. Some of the abbreviations used are as follows: RT or rt for room temperature or retention time (context will dictate); ret t for retention time; min or mins for minutes; TFA for trifluoro acetic acid; min or mins for minutes; ACN or MeCN for acetonitrile; DCM for dichloromethane; DIEA or DiPEA or DIPEA for

diisopropylethylamine; HATU for 0-(7-azabenzotriazol-l-yl)-N,N,N',N'- tetramethyluronium hexafluorphosphate; h or hr or hrs for hours; MeOH for methanol; dppf for diphenylphosphinoferrocene; EtOAc for ethyl acetate; OAc for acetate; DMSO for dimethylsulfoxide; TBTU for 2-(lH-benzotriazole-l-yl)-l, 1,3,3- tetramethyluronium tetrafluoroborate; Me for methyl; and DMF for N,N- dimethy lformamide .

The present disclosure will now be described in connection with certain embodiments which are not intended to limit its scope. On the contrary, the present disclosure covers all alternatives, modifications, and equivalents as can be included within the scope of the claims. Thus, the following examples, which include specific embodiments, will illustrate one practice of the present disclosure, it being understood that the examples are for the purposes of illustration of certain embodiments and are presented to provide what was believed to be the most useful and readily understood description of its procedures and conceptual aspects.

Starting materials can be obtained from commercial sources or prepared by well-established literature methods known to those of ordinary skill in the art. Acid precursors for the final step can be prepared according to the methods described in U.S. Patent Application Serial No. 13/933495, filed July 2, 2013.

LC/MS Condition 1

Column = Ascentis Express C18, 2.1 X 50 mm, 2.7 um

Solvent A = CH₃CN (2%) + 10 mM NH₄COOH in H₂0 (98%)

Solvent B = CH₃CN (98%) + 10 mM NH₄COOH in H₂0 (2%)

Start %B = 0; Final %B = 100

Gradient time = 1.4 min; Stop time = 4 min

Stop time = 4 min

Flow Rate = 1 mL/min; Wavelength = 220 nm LC/MS Condition 2

Column = Waters BEH CI 8, 2.0 x 50 mm, 1.7 μιη

Slovent A = ACN (5%) + H₂0 (95%) containing 10 mM NH₄OAc

Solvent B = ACN (95%) + H₂0 (5%) containing 10 mM NH₄OAc

Start %B = 0; Final %B = 100

Gradient time = 3 min

Flow Rate = 1 mL/min

Wavelength = 220 nm

Temperature = 50 °C

LC/MS Condition 3

Column: Waters Phenomenex CI 8, 2.0 x 30 mm, 3 μιη particle

Mobile Phase A: 10% MeOH:90% Water :0.1%TFA

Mobile Phase B: 90% MeOH: 10% Water :0.1%TFA

Gradient: 0%B, 0-100% B over 3 minutes, then a 1 -minute hold at 100% B Flow: 0.8mL/min

Detection: 220 nm

Temperature: 40 °C LC/MS Condition 4

Column: Waters BEH CI 8, 2.0 x 50 mm, 1.7 μιη particle

Mobile Phase A: 5:95 acetonitrile: water with 10 mM ammonium acetate Mobile Phase B: 95:5 acetonitrile: water with 10 mM ammonium acetate Gradient: 0%B, 0-100% B over 3 minutes, then a 0.5-minute hold at 100% B Flow: 1 mL/min

Detection: UV at 220 nm

Temperature: 50 °C

Example B-l

Example B-l Step a

To a solution of 4-bromobenzene-l,2-diamine (2.5 g, 13.37 mmol) in DCM (30 mL) was added (S)-2-((tert-butoxycarbonyl)amino)-3,3-dimethylbutanoic acid (3.09 g, 13.37 mmol), DIPEA (2.334 mL, 13.37 mmol) and HATU (5.08 g, 13.37 mmol). The reaction mixture was stirred at room temperature for 18 h. The reaction mixture was diluted with water and extracted with DCM. The organic phase was washed with brine, dried over Na₂S0₄, filtered and concentrated. The crude material was purified by ISCO using 40 g Redisep silica column, CHCl₃/MeOH as eluant to obtain (S)-tert-butyl ( 1 -((2-amino-4-bromophenyl)amino)-3 ,3 -dimethyl- 1 -oxobutan- 2-yl) carbamate (1.82 g) as yellow solid. LC (Condition 1): R_t = 2.13 min. LC/MS: Anal. Calcd. for [M+H₂0]⁺ Ci₇H₂₇BrN₂0₄ : 402.12; found 402.2. 1H NMR (DMSO- d₆, δ = 2.50 ppm, 400 MHz): δ 9.35 - 9.21 (m, 1 H), 7.07 (d, J= 8.5 Hz, 1 H), 6.91 (d, J= 2.0 Hz, 1 H), 6.80 - 6.60 (m, 1 H), 5.25 - 5.01 (m, 2 H), 4.07 - 3.89 (m, 1 H), 1.52 - 1.34 (m, 9 H), 1.02 - 0.86 (m, 9 H).

Example B-l, Step b

Acetic acid (15 mL) was added to (S)-tert-butyl (l-((2-amino-4-bromo phenyl)amino)-3,3-dimethyl-l-oxobutan-2-yl)carbamate (1.8 g, 4.50 mmol) and the reaction mixture was heated to 65 °C for overnight. The volatile component was removed in vacuo, and the residue was co-evaporated with dry CH₂C1₂ (2 x 15 mL). The organic phase was washed with saturated NaHC0₃ solution, brine, dried over Na₂S0₄ and concentrated to obtain (S)-tert-butyl (l-(6-bromo-lH-benzo[d] imidazol- 2-yl)-2,2-dimethyl propyl)carbamate (1.68 g) as yellow solid. LC (Condition 1): R_t = 2.19 min. LC/MS: Anal. Calcd. for [M+H]⁺ Ci₇H₂₅BrN₃0₂ : 381.11; found 382.2. 1H NMR (DMSO-dg, δ = 2.50 ppm, 300 MHz): δ 12.46 - 12.27 (m, 1 H), 7.82 - 7.65 (m, 1 H), 7.59 - 7.41 (m, 1 H), 7.29 (dt, J= 1.9, 8.5 Hz, 1 H), 7.12 - 6.90 (m, 1 H), 4.64 (d, J= 9.8 Hz, 1 H), 1.44 - 1.27 (m, 9 H), 0.88 (br. s., 9 H). -1 Step c

To a solution of (S)-tert-butyl (l-(6-bromo-lH-benzo[d]imidazol-2-yl)-2,2- dimethyl propyl) carbamate (1.57 g, 4.11 mmol) in dioxane (25 mL) was added bis (pinacolato)diboron (1.564 g, 6.16 mmol) and potassium acetate (1.209 g, 12.32 mmol). The reaction mixture was purged with argon for 10 min then PdCl₂(dppf) (0.150 g, 0.205 mmol) was added to the above reaction mixture and again purged with argon for 5 min. The reaction mixture was heated to 90 °C for overnight. The reaction mixture was diluted with water (15 ml) and extracted with EtOAc (2 x 25 ml). The combined organic phase was washed with brine, dried over Na₂S0₄ and concentrated in vacuo. The crude material was purified by ISCO using 40 g Redisep column, hexane/ethyl acetate as eluant to afford (S)-tert-butyl (2,2-dimethyl-l-(6- (4,4,5 ,5-tetramethyl- 1 ,3 ,2-dioxaborolan-2-yl)- 1 H-benzo[d]imidazol-2-yl)propyl) carbamate (1.35 g) as yellow solid. LC (Condition 1): R_t = 2.21 min. LC/MS: Anal. Calcd. for [M+H]⁺ C₂₃H₃₇BN₃0₄ : 430.29; found 430.4. 1H NMR (CD₃OD, δ = 3.34 ppm, 400 MHz): δ 7.98 (s, 1 H), 7.65 (dd, J= 1.0, 8.5 Hz, 1 H), 7.53(d, J= 8.5 Hz, 1 H), 4.73 (br. s., 1 H), 1.37 (s, 12 H), 1.24 (m, 9 H), 1.01 (s, 9 H). -1 Step d

To a solution of (S)-tert-butyl (2,2-dimethyl-l-(6-(4,4,5,5-tetramethyl-l,3,2- dioxaborolan-2-yl)-lH-benzo[d]imidazol-2-yl)propyl)carbamate (1.114 g, 2.59 mmol) and 4,16-dibromo[2,2]paracyclophane (0.38g, 1.038 mmol) in dioxane (10 mL) was added Cs₂C0₃ (0.845 g, 2.59 mmol) in water (2 mL) and degassed for 10 min.

PdCl₂(dppf) (0.038 g, 0.052 mmol) was added to the above reaction mixture and again degassed for 5 min. The reaction mixture was heated to 90 °C for 12 h. Then the reaction mixture was filtered to get Example B-1 Step d which was taken for next step without further purification. LC (Condition 1): R_t = 2.54 min. LC/MS: Anal. Calcd. for [M+H]⁺ ^₀Η₆₃Ν₆Ο₄ : 811.49; found 811.6. 1H NMR (DMSO-d₆, δ = 2.50 ppm, 300 MHz): δ 12.36 (br. s., 2 H), 7.85 - 7.52 (m, 4 H), 7.32 (d, J= 7.9 Hz, 2 H), 7.05 (br. s., 2 H), 6.89 - 6.67 (m, 4 H), 6.54 (br. s., 2 H), 4.72 (d, J= 8.7 Hz, 2 H), 3.57 - 3.44 (m, 2 H), 3.07 (br. s., 2 H), 2.83 (br. s., 2 H), 2.65 (br. s., 2 H), 1.36 (s, 18 H), 1.08 - 0.91 (m, 18 H). -1 Step e

HC1 in dioxane (4 mL, 24.00 mmol) was added to Example B-1 Step d (0.1 g,

0.102 mmol), and the reaction mixture was allowed to stir at RT for 2 h. Completion of the reaction was monitored by LCMS. The volatile component was removed in vacuo and the residue was washed with diethyl ether and dried to afford Example B-1 Step e (0.07 g) as yellow solid. LC (Condition 1): R, = 2.54 min. LC/MS: Anal.

Calcd. for [M+H]⁺ C40H47N6 : 611.39; found 611.4. 1H NMR (CD₃OD, δ = 3.34 ppm, 400 MHz): δ 7.90 (d, J= 13.1 Hz, 2 H), 7.83 (d, J= 8.5 Hz, 2 H), 7.61 (d, J= 8.5 Hz, 2 H), 6.84 (d, J= 6.5 Hz, 2 H), 6.78 (s, 2 H), 6.70 - 6.65 (m, 2 H), 4.54 (d, J= 1.0 Hz, 2 H), 3.54 - 3.46 (m, 2 H), 3.18 - 3.10 (m, 2 H), 2.98 - 2.86 (m, 2 H), 2.71 (br. s., 2 H), 1.25 - 1.22 (m, 18 H).

To a solution of Example B-1 Step e (0.04 g, 0.053 mmol) in DMF (5 mL) was added 4,4-difluorocyclohexanecarboxylic acid (0.017 g, 0.106 mmol), DIPEA (0.055 mL, 0.317 mmol) and HATU (0.030 g, 0.079 mmol). After being stirred for 2 h at room temperature, the volatile component was removed in vacuo and the residue was dissolved in DCM (10 mL), washed with saturated solution of NH₄C1, 10% NaHC0₃ solution, brine, dried over Na₂S0₄ and concentrated in vacuo. The crude was purified by reverse phase HPLC purification to give Example B-1 as a white solid. LC (Condition 1): R, = 2.37 min. LC/MS: Anal. Calcd. for [M+H]⁺

C₅₄H₆₃F₄N₆0₂: 903.49; found 903.4. 1H NMR (DMSO-d₆, δ = 2.50 ppm, 400 MHz): δ 12.53 - 12.32 (m, 2 H), 8.41 - 8.21 (m, 2 H), 7.84 - 7.50 (m, 4 H), 7.43 - 7.24 (m, 2 H), 6.90 - 6.67 (m, 4 H), 6.60 - 6.44 (m, 2 H), 5.14 - 4.97 (m, 2 H), 3.44 (br. s., 2 H), 3.08 (br. s., 2 H), 2.93 - 2.77 (m, 2 H), 2.73 - 2.56 (m, 4 H), 2.20 - 1.98 (m, 3 H), 1.96 - 1.49 (m, 13 H), 1.02 (s, 18 H).

The following examples were prepared from Example B-1 Step e, and appropriate acid precursors by employing the procedures described for the synthesis of Example B-1. The resulting products were purified by preparatory HPLC

13.6 Hz, 2 H), 1.29 (s, 9 H), 1.28 (s, 9 H), 1.09 (s,

18 H)

LC (Condition 2): R, = 2.52 min. LC/MS: Anal.

Calcd. for [M+H]⁺ C₅₄H63F₄N60₄: 935.48; found

935.47.

1H NMR (600 MHz, DMSO-d₆) δ 12.68 - 12.51

(m, 2H), 7.78 (m, 3H), 7.74 - 7.68 (m, 1H), 7.65 -

7.57 (m, 2H), 7.37 - 7.29 (m, 2H), 6.83 - 6.74 (m,

B-4 2H), 6.73 - 6.66 (m, 2H), 6.56 - 6.45 (m, 2H), 5.14

- 5.02 (m, 2H), 3.98 - 3.85 (m, 3H), 3.83 - 3.73 (m,

F F 2H), 3.11 - 2.99 (m, 2H), 2.86 - 2.76 (m, 2H), 2.69

- 2.55 (m, 2H), 2.13 (br. s., 4H), 1.88 - 1.75 (m,

2H), 1.74 - 1.64 (m, 2H), 1.46/1.45 (2 s, 6H),

1.01/0.99 (2 s, 18H).

LC (Condition 2): R, = 2.44 min. LC/MS: Anal.

Calcd. for [M+H]⁺ 941.47; found

941.50.

1H NMR (600 MHz, DMSO-d₆) δ 12.61 - 12.46

(m, 2H), 8.41 - 8.33 (m, 2H), 7.87 - 7.75 (m, 1H),

Vi 7.73 (dd, J=13.0, 8.3 Hz, 1H), 7.63 - 7.54 (m, 2H), o 7.37 - 7.33 (m, 1H), 7.29 (t, J=8.1 Hz, 1H), 6.87 -

NH

B-5 6.80 (m, 1H), 6.79 - 6.70 (m, 3H), 6.56 - 6.48 (m,

<

CF₃ 2H), 5.02 (br. d, J=9.5 Hz, 2H), 3.55 - 3.46 (m,

2H), 3.22 (d, J=7.9 Hz, 2H), 3.17 (d, J=5.1 Hz,

1H), 3.06 - 3.05 (m, 1H), 3.12 - 3.02 (m, 2H), 2.87

- 2.79 (m, 2H), 2.66 - 2.58 (m, 2H), 1.20 - 1.13 (m,

2H), 1.04 - 0.95 (m, 24H) -l to Y-5

Step-b

Boc R = H Step-c R = COR'

(HCI salt)

1c Y-1

Y-1d

Step a:

To a solution of boronate Y-la (0.80 g, 1.93 mmol), cyclophane Y-lb (0.25 g,

0.683 mmol) in dioxane (8 mL) was added CS2CO3 (0.70 g, 2.148 mmol) in water (2 mL), the mixture was degassed for 10 min and PdCl₂(dppf) (0.025 g, 0.034 mmol) was added to the above reaction mixture and again degassed for 5 min. Reaction mixture was heated to 90 °C for 18h, cooled to ambient temperature, diluted with EtOAc and filtered, and dried to afford Y-lc as a grey solid (0.45g). LC (Condition 3): R, = 2.79 min. LC/MS: Anal. Calcd. for C₄8H₅₈N₆0₄: 782.45; found 783.61

[M+H]⁺.

Step b:

Bis-carbamate Y-lc was deprotected to Y-l d (HCI salt) according to the procedure described in Example 1 step e. LC (Condition 3): R_t = 2.79 min. LC/MS: Anal. Calcd. for C₃₈H₄₂N₆-4 HCI: 582.78; found 583.45 [M+H]⁺.

Step c:

To a mixture of Y-ld (HCI salt) (30 mg, 0.041 mmol) and 4,4- difluorocyclohexanecarboxylic acid (18 mg, 0.110 mmol) in DCM (1 mL) and acetonitrile (1 mL) was added DIPEA (0.1 mL, 0.573 mmol) and TBTU (30 mg, 0.093 mmol). The reaction mixture was stirred at RT for 45 min, diluted with MeOH (lmL), concentrated and purified by prep HPLC (H₂0/CH₃CN/NH₄OAc) to afford Example Y-l . Example Y-2 to Y-5 were prepared similarly from appropriate carboxylic acid precursors, the synthesis of which is reported in the art. Note that the acid precursor used in the preparation of Example Y-4 was a racemate mixture with its hydroxyl and carboxyl moieties cis to each other.

Example R

LC (Condition 4): R, = 2.28

min. LC/MS: Anal. Calcd.

Y-l

for C₅2H₅8F4N60₂: 874.456 ;

found 876.0 [M+H]⁺

LC (Condition 4): R, = 2.35

min. LC/MS: Anal. Calcd.

Y-2

for C₄8H₅8N60₂: 750.46;

found 751.46. [M+H]⁺

LC (Condition 4): R, = 2.42

min. LC/MS: Anal. Calcd.

Y-3 for C₅₂H₅8F₄N₆0₄: 906.446 ;

found, 907.45 [M+H]⁺

LC (Condition 4): R, = 2.26

Y-4

min. LC/MS: Anal. Calcd.

(Mixture of

for C₅₄H₆6N₆0: 862.515;

diastereomers) (OH & Me are

found 864.1 [M+H]⁺

cis)

LC (Condition 4): R, = 2.79

0 min. LC/MS: Anal. Calcd.

Y-5 \

for C₅₀H6₂N₆O₄: 810.483;

found, 81 1.65 [M+H]⁺

BIOLOGICAL ACTIVITY

The NS5A synergistic inhibitory effect of test compounds can be determined using various amounts of an NS5A-targeting compound with titration of a second compound of interest. Both the NS5 A-targeting compound and the second compound of interest, when tested individually versus HCV variants, are understood to be essentially inactive or weakly active and only regain synergistic inhibitory potency of 3 -fold or greater inhibition when tested in combination versus HCV variants. In one embodiment, compound BMS-790052, as an NS5A-targeting compound, can be held constant at a fixed concentration of 200nM with subsequent titration of the test compound on a variant of HCV. In one embodiment, the HCV genotype strain can be genotype la containing a change at amino acid 30 of the NS5A protein consisting of glutamine to glutamate. The test compound can be chosen from compounds listed above or from others present in the literature. One skilled in the art can readily test compounds in the HCV replicon cell based assay as has been demonstrated previously in the art and one can readily determine the effective concentration for 50% inhibition (EC₅₀) of a particular compound.

For illustration, Compound P-55, which is noted below, can be titrated in the HCV replicon cell-based assay consisting of the genotype- la variant with glutamine 30 changed to glutamate in the NS5A protein. Titration of BMS-790052 singly would yield an EC₅₀ value ~200nM while titration of P-55 singly would yield an EC₅₀ value >200nM. The titration of P-55 in the presence of a fixed amount of BMS- 790052 at 200nM afforded an EC₅₀ values of ~2 nM for P-55 demonstrating a synergistic inhibitory effect for the combination of > 100-fold. Similarly, the titration of BMS-790052 in the presence of a fixed amount of P-55 at 200nM afforded an EC₅₀ values of ~2 nM for BMS-790052, demonstrating a reciprocal synergistic inhibitory effect ~100-fold for the combination (PCT/US2011/043785, filed July 13, 2011), Table 3). Additional compounds can be tested in a similar manner and a ranking of synergist activities determined; these rankings for the genotype la Q- E variant are shown for selected compounds in the table below.

It is understood that the genotype is not limited to the genotype la variant but can encompass all genotypic variants of HCV including but not limited to HCV variants of lb, 2a, 3a, 4a, 5a, 6a as demonstrated in commonly owned

WO2012/009394. It is also understood that the synergy effect is not limited to BMS- 790052 or P-55 combinations but can be derived from other combinations of NS5A- targeting compounds that by themselves have reduced or no potency towards HCV variants.

It will be evident to one skilled in the art that the present disclosure is not limited to the foregoing illustrative examples, and that it can be embodied in other specific forms without departing from the essential attributes thereof. It is therefore desired that the examples be considered in all respects as illustrative and not restrictive, reference being made to the appended claims, rather than to the foregoing examples, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.

Claims

WHAT IS CLAIMED IS:

1. A combination comprising an NS5 A-targeting compound and an NS5 A synergist, which, when administered, provides synergistic anti-HCV activity against variants that contain mutation(s) conferring resistance to the NS5A-targeting compound alone, wherein the NS5A synergist is a compound of formula (I):

(I),

or a pharmaceutically acceptable salt thereof, wherein

R^1' and R^1' are independently selected from alkoxyalkyl, alkyl, cycloalkyl, and pyranyl, wherein the cycloalkyl and the pyranyl are optionally substituted with one, two, or three substituents independently selected from alkyl, halo, haloalkyl, hydroxy, and hydroxyalkyl; and

R² and R^2' are the same or different alkyl groups.

The combination of claim 1 wherein the compound of formula (I) is selected

or a pharmaceutically acceptable salt thereof.

3. A composition comprising the combination of claim 1 and one or more pharmaceutically acceptable carriers. 4. The composition of claim 3 further comprising one or two additional compounds having anti-HCV activity.

5. The composition of claim 4 wherein at least one of the additional compounds is an interferon or a ribavirin.

6. The composition of claim 5 wherein the interferon is selected from interferon alpha 2B, pegylated interferon alpha, pegylated interferon lambda, consensus interferon, interferon alpha 2A, and lymphoblastoid interferon tau.

7. The composition of claim 4 wherein at least one of the additional compounds is effective to inhibit the function of a target selected from HCV protease, HCV polymerase, HCV helicase, HCV NS4B protein, HCV entry, HCV assembly, HCV egress, HCV NS5A protein, and IMPDH for the treatment of an HCV infection.

8. A method of treating an HCV infection in a patient, comprising administering to the patient a therapeutically effective amount of a combination of claim 1 , or a pharmaceutically acceptable salt thereof. 9. The method of claim 8 further comprising administering one or two additional compounds having anti-HCV activity prior to, after or simultaneously with the combination, or a pharmaceutically acceptable salt thereof.

10. The method of claim 9 wherein at least one of the additional compounds is an interferon or a ribavirin.

11. The method of claim 10 wherein interferon is selected from interferon alpha 2B, pegylated interferon alpha, pegylated interferon lambda, consensus interferon, interferon alpha 2A, and lymphoblastoid interferon tau.

12. The method of claim 9 wherein at least one of the additional compounds is effective to inhibit the function of a target selected from HCV protease, HCV polymerase, HCV helicase, HCV NS4B protein, HCV entry, HCV assembly, HCV egress, HCV NS5A protein, and IMPDH for the treatment of an HCV infection.