EP2971165A4 - Removal of dna fragments in mrna production process - Google Patents
Removal of dna fragments in mrna production processInfo
- Publication number
- EP2971165A4 EP2971165A4 EP14770821.8A EP14770821A EP2971165A4 EP 2971165 A4 EP2971165 A4 EP 2971165A4 EP 14770821 A EP14770821 A EP 14770821A EP 2971165 A4 EP2971165 A4 EP 2971165A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- removal
- production process
- dna fragments
- mrna production
- mrna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361799872P | 2013-03-15 | 2013-03-15 | |
| PCT/US2014/026838 WO2014152030A1 (en) | 2013-03-15 | 2014-03-13 | Removal of dna fragments in mrna production process |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EP2971165A1 EP2971165A1 (en) | 2016-01-20 |
| EP2971165A4 true EP2971165A4 (en) | 2016-11-23 |
Family
ID=51581100
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP14770821.8A Withdrawn EP2971165A4 (en) | 2013-03-15 | 2014-03-13 | Removal of dna fragments in mrna production process |
Country Status (3)
| Country | Link |
|---|---|
| US (3) | US10077439B2 (en) |
| EP (1) | EP2971165A4 (en) |
| WO (1) | WO2014152030A1 (en) |
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| CN106794141B (en) | 2014-07-16 | 2021-05-28 | 诺华股份有限公司 | Method for encapsulating nucleic acids in lipid nanoparticle hosts |
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| EP3169335B8 (en) | 2014-07-16 | 2019-10-09 | ModernaTX, Inc. | Circular polynucleotides |
| EP2992958A1 (en) | 2014-09-03 | 2016-03-09 | STAT-Diagnostica D Innovation SL | Nucleic acid purification cartridge |
| US20170348415A1 (en) | 2014-09-03 | 2017-12-07 | Moderna Therapeutics, Inc. | Tolerogenic compositions and methods |
| WO2016077125A1 (en) | 2014-11-10 | 2016-05-19 | Moderna Therapeutics, Inc. | Alternative nucleic acid molecules containing reduced uracil content and uses thereof |
| EP3247363A4 (en) | 2015-01-21 | 2018-10-03 | Moderna Therapeutics, Inc. | Lipid nanoparticle compositions |
| US20180085474A1 (en) | 2015-01-23 | 2018-03-29 | Moderna Therapeutics, Inc. | Lipid nanoparticle compositions |
| WO2016180430A1 (en) | 2015-05-08 | 2016-11-17 | Curevac Ag | Method for producing rna |
| CA3002922A1 (en) | 2015-10-22 | 2017-04-27 | Modernatx, Inc. | Human cytomegalovirus vaccine |
-
2014
- 2014-03-13 US US14/777,301 patent/US10077439B2/en active Active
- 2014-03-13 EP EP14770821.8A patent/EP2971165A4/en not_active Withdrawn
- 2014-03-13 WO PCT/US2014/026838 patent/WO2014152030A1/en active Application Filing
-
2018
- 2018-07-30 US US16/049,132 patent/US10858647B2/en active Active
-
2020
- 2020-12-07 US US17/113,940 patent/US20210230578A1/en not_active Abandoned
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| BHAIRAVI JANI ET AL: "In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection", JOVE, JOURNAL OF VISUALIZED EXPERIMENTS, no. 61, E3702, 26 March 2012 (2012-03-26), US, pages 1 - 9, XP055307725, ISSN: 1940-087X, DOI: 10.3791/3702 * |
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Also Published As
| Publication number | Publication date |
|---|---|
| US20190100748A1 (en) | 2019-04-04 |
| WO2014152030A1 (en) | 2014-09-25 |
| US10858647B2 (en) | 2020-12-08 |
| US20160024492A1 (en) | 2016-01-28 |
| US20210230578A1 (en) | 2021-07-29 |
| US10077439B2 (en) | 2018-09-18 |
| EP2971165A1 (en) | 2016-01-20 |
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