EP2940032A4 - Extraction method for hydrophilic recombinant protein - Google Patents

Extraction method for hydrophilic recombinant protein

Info

Publication number
EP2940032A4
EP2940032A4 EP13867712.5A EP13867712A EP2940032A4 EP 2940032 A4 EP2940032 A4 EP 2940032A4 EP 13867712 A EP13867712 A EP 13867712A EP 2940032 A4 EP2940032 A4 EP 2940032A4
Authority
EP
European Patent Office
Prior art keywords
recombinant protein
extraction method
hydrophilic recombinant
hydrophilic
extraction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP13867712.5A
Other languages
German (de)
French (fr)
Other versions
EP2940032B1 (en
EP2940032A1 (en
Inventor
Toshiaki Osawa
Keisuke Morita
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Spiber Inc
Original Assignee
Spiber Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Spiber Inc filed Critical Spiber Inc
Publication of EP2940032A1 publication Critical patent/EP2940032A1/en
Publication of EP2940032A4 publication Critical patent/EP2940032A4/en
Application granted granted Critical
Publication of EP2940032B1 publication Critical patent/EP2940032B1/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Insects & Arthropods (AREA)
  • Analytical Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
EP13867712.5A 2012-12-27 2013-12-18 Extraction method for hydrophilic recombinant protein Active EP2940032B1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2012285392 2012-12-27
JP2013093934 2013-04-26
PCT/JP2013/083971 WO2014103846A1 (en) 2012-12-27 2013-12-18 Extraction method for hydrophilic recombinant protein

Publications (3)

Publication Number Publication Date
EP2940032A1 EP2940032A1 (en) 2015-11-04
EP2940032A4 true EP2940032A4 (en) 2016-10-12
EP2940032B1 EP2940032B1 (en) 2018-04-25

Family

ID=51020933

Family Applications (1)

Application Number Title Priority Date Filing Date
EP13867712.5A Active EP2940032B1 (en) 2012-12-27 2013-12-18 Extraction method for hydrophilic recombinant protein

Country Status (5)

Country Link
US (1) US20150329587A1 (en)
EP (1) EP2940032B1 (en)
JP (1) JP6077569B2 (en)
CN (1) CN104884463B (en)
WO (1) WO2014103846A1 (en)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6077570B2 (en) * 2012-12-27 2017-02-08 Ｓｐｉｂｅｒ株式会社 Crude purification method for hydrophilic recombinant protein
JP5796147B2 (en) 2013-04-25 2015-10-21 Ｓｐｉｂｅｒ株式会社 Polypeptide porous body and method for producing the same
EP2990062B1 (en) 2013-04-25 2021-11-10 Spiber Inc. Polypeptide hydrogel and method for producing same
EP3281949A4 (en) * 2015-04-09 2018-08-22 Spiber Inc. Polar solvent solution and production method thereof
US20180080147A1 (en) 2015-04-09 2018-03-22 Spiber Inc. Polar solvent solution and production method thereof
US20180251501A1 (en) * 2015-09-18 2018-09-06 Spiber Inc. Molded Article and Method for Producing Molded Article
EP3351584B1 (en) * 2015-09-18 2021-04-07 Spiber Inc. Molded article and method for producing molded article
EP3498856A4 (en) 2016-08-10 2020-05-06 Spiber Inc. Production method for insoluble recombinant protein aggregate
WO2018066558A1 (en) 2016-10-03 2018-04-12 Ｓｐｉｂｅｒ株式会社 Method for purifying recombinant protein
JP2020097524A (en) * 2017-03-07 2020-06-25 Ｓｐｉｂｅｒ株式会社 Method for producing purified protein
JP7320846B2 (en) * 2017-11-10 2023-08-04 ジボダン エス エー Silk alcohol preparation
JP2021151953A (en) * 2018-03-30 2021-09-30 独立行政法人国立高等専門学校機構 Method for producing recombinant-structure protein, recombinant-structure protein, protein molding, and method for producing protein molding
US11178934B2 (en) * 2018-07-18 2021-11-23 Bolt Threads Inc. Resilin material footwear and fabrication methods
JPWO2020067572A1 (en) 2018-09-28 2021-03-18 Ｓｐｉｂｅｒ株式会社 Method for producing protein composition
JP7308507B2 (en) * 2019-01-31 2023-07-14 Ｓｐｉｂｅｒ株式会社 Method for producing and analyzing recombinant structural protein, and method for producing protein compact
JP2022548481A (en) * 2019-09-16 2022-11-21 ボルト スレッズ インコーポレイテッド A Method for Isolating Spider Silk Proteins Via High Shear Solubilization
WO2021066078A1 (en) 2019-09-30 2021-04-08 Ｓｐｉｂｅｒ株式会社 Muscle tissue-regenerating agent
JPWO2021065794A1 (en) 2019-09-30 2021-04-08

Citations (3)

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Publication number Priority date Publication date Assignee Title
US20020081286A1 (en) * 2000-05-23 2002-06-27 Mark Marchionni NRG-2 nucleic acid molecules, polypeptides, and diagnostic and therapeutic methods
WO2002096931A1 (en) * 2001-05-25 2002-12-05 Proteome Systems Intellectual Property Pty Ltd Increased solubilisation of hydrophobic proteins
US20050158821A1 (en) * 2000-01-20 2005-07-21 Charlene Mello Methods for the purification and aqueous fiber spinning of spider silks and other structural proteins

Family Cites Families (10)

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Publication number Priority date Publication date Assignee Title
US4448750A (en) * 1981-06-05 1984-05-15 Fuesting Michael L Sterilization method
JPS61200996A (en) * 1985-03-04 1986-09-05 Agency Of Ind Science & Technol Production of organic electrically-conductive material crystal
ES2344189T3 (en) * 1999-09-03 2010-08-20 RAMOT UNIVERSITY AUTHORITY FOR APPLIED RESEARCH & INDUSTRIAL DEVELOPMENT LTD. AGENTS, COMPOSITIONS AND METHODS THAT USE THEM USEFUL IN THE TREATMENT OR PREVENTION OF ALZHEIMER'S DISEASE.
EP2220112B1 (en) * 2007-11-26 2015-08-26 Yissum Research Development Company of the Hebrew University of Jerusalem Ltd. Compositions comprising fibrous polypeptides and polysaccharides
WO2011075527A1 (en) * 2009-12-18 2011-06-23 Stokely-Van Camp, Inc. Protein recovery beverage
EP2560683B2 (en) * 2010-04-23 2022-07-20 F. Hoffmann-La Roche AG Production of heteromultimeric proteins
JP2012012332A (en) * 2010-06-30 2012-01-19 Dainippon Sumitomo Pharma Co Ltd New azaindole derivative
EP2774934B1 (en) * 2011-11-02 2017-11-29 Spiber Inc. Polypeptide solution, artificial polypeptide fiber production method using same, and polypeptide refining method
US9439972B2 (en) * 2012-09-10 2016-09-13 Ad Lunam Labs, Inc. Antifungal serum
US10875024B2 (en) * 2014-07-10 2020-12-29 The Board Of Regents Of The University Of Texas System Methods and compositions for paper-based and hybrid microfluidic devices integrated with nucleic acid amplification for disease diagnosis

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050158821A1 (en) * 2000-01-20 2005-07-21 Charlene Mello Methods for the purification and aqueous fiber spinning of spider silks and other structural proteins
US20020081286A1 (en) * 2000-05-23 2002-06-27 Mark Marchionni NRG-2 nucleic acid molecules, polypeptides, and diagnostic and therapeutic methods
WO2002096931A1 (en) * 2001-05-25 2002-12-05 Proteome Systems Intellectual Property Pty Ltd Increased solubilisation of hydrophobic proteins

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
"IUPAC Compendium of Chemical Terminology", 24 February 2014, IUPAC, Research Triagle Park, NC, ISBN: 978-0-9678550-9-7, article M. NIC ET AL: "dipolar aprotic solvent", XP055299347, DOI: 10.1351/goldbook.D01751 *
CHANG N ET AL: "Protein separation and purification in neat dimethyl sulfoxide", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 176, no. 3, 15 May 1991 (1991-05-15), pages 1462 - 1468, XP024835967, ISSN: 0006-291X, [retrieved on 19910515], DOI: 10.1016/0006-291X(91)90451-C *
See also references of WO2014103846A1 *

Also Published As

Publication number Publication date
CN104884463A (en) 2015-09-02
EP2940032B1 (en) 2018-04-25
EP2940032A1 (en) 2015-11-04
JP6077569B2 (en) 2017-02-08
JPWO2014103846A1 (en) 2017-01-12
CN104884463B (en) 2018-09-18
US20150329587A1 (en) 2015-11-19
WO2014103846A1 (en) 2014-07-03

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