EP2855296A1 - Device for the collection, pre-analytic treatment, transport and grinding of solid samples - Google Patents
Device for the collection, pre-analytic treatment, transport and grinding of solid samplesInfo
- Publication number
- EP2855296A1 EP2855296A1 EP13737408.8A EP13737408A EP2855296A1 EP 2855296 A1 EP2855296 A1 EP 2855296A1 EP 13737408 A EP13737408 A EP 13737408A EP 2855296 A1 EP2855296 A1 EP 2855296A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- medium
- preanalytical
- balls
- retaining means
- grinding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D23/00—Details of bottles or jars not otherwise provided for
- B65D23/04—Means for mixing or for promoting flow of contents
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
- B01F31/20—Mixing the contents of independent containers, e.g. test tubes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/25—Mixers with loose mixing elements, e.g. loose balls in a receptacle
- B01F33/251—Mixers with loose mixing elements, e.g. loose balls in a receptacle using balls as loose mixing element
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/45—Magnetic mixers; Mixers with magnetically driven stirrers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C17/00—Disintegrating by tumbling mills, i.e. mills having a container charged with the material to be disintegrated with or without special disintegrating members such as pebbles or balls
- B02C17/04—Disintegrating by tumbling mills, i.e. mills having a container charged with the material to be disintegrated with or without special disintegrating members such as pebbles or balls with unperforated container
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D51/00—Closures not otherwise provided for
- B65D51/24—Closures not otherwise provided for combined or co-operating with auxiliary devices for non-closing purposes
- B65D51/28—Closures not otherwise provided for combined or co-operating with auxiliary devices for non-closing purposes with auxiliary containers for additional articles or materials
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D51/00—Closures not otherwise provided for
- B65D51/24—Closures not otherwise provided for combined or co-operating with auxiliary devices for non-closing purposes
- B65D51/28—Closures not otherwise provided for combined or co-operating with auxiliary devices for non-closing purposes with auxiliary containers for additional articles or materials
- B65D51/2807—Closures not otherwise provided for combined or co-operating with auxiliary devices for non-closing purposes with auxiliary containers for additional articles or materials the closure presenting means for placing the additional articles or materials in contact with the main contents by acting on a part of the closure without removing the closure, e.g. by pushing down, pulling up, rotating or turning a part of the closure, or upon initial opening of the container
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/32—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents for packaging two or more different materials which must be maintained separate prior to use in admixture
- B65D81/3233—Flexible containers disposed within rigid containers
- B65D81/3238—Flexible containers disposed within rigid containers with additional means facilitating admixture
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
- B01F2101/23—Mixing of laboratory samples e.g. in preparation of analysing or testing properties of materials
Definitions
- the invention relates to a device for the collection, pre-analytical processing, transport and grinding of solid samples, for subsequent analysis, whether a biological, genetic, chemical or microbiological analysis or extraction of nucleic acids, proteins or other organic or inorganic compounds.
- This device can be used in many areas, such as the medical, veterinary or environmental, for the analysis of pollutants in particular.
- sterile tubes In the case of blood samples for example, they are placed in sterile tubes. These tubes are usually in depression and may have, on their inner surface or in solution, a particular product, chosen according to the subsequent analysis.
- vacuum sampling devices containing a culture or transport medium are widely used.
- Liquid sample analysis machines are widely used. Moreover, thanks to the properties of the sampling tubes, the liquid samples do not in most cases require any manipulation prior to the analysis except for a possible step of centrifugation. They can therefore be analyzed very quickly, without risk of degradation and without risk of error or contamination introduced by the manipulation or opening of the sampling device.
- infections on orthopedic equipment and in particular on joint implants are difficult to diagnose due to the small amount of microorganisms present and their metabolism adapted to survival within a biofilm on the surface of the body. implant or inside the cells present at the site of the infection.
- pathogens encountered are part of the cutaneous flora and are indistinguishable from the usual contaminants encountered in clinical microbiology, such as Staphylococcus spp., Corynebacterium or Propionibacterium.
- the sensitivity is defined by the ratio of cases detected positive / cases actually positive and specificity by the ratio of cases detected to negative / cases actually negative.
- sample bottle containing no solid or liquid adjuvant. It is, in some cases, placed in a transport medium intended to ensure the survival of microorganisms or the stability of nucleic acids, proteins or any analyte until the analysis is performed.
- This sample (bone, muscle, tendon, viscera, implanted material or other) solid, viscous or heterogeneous is then transmitted to the laboratory.
- a solid sample it is transferred into a device for extracting microorganisms or genetic or protein material in a liquid phase compatible with the biological analysis performed downstream.
- the most commonly used devices are traditional mortars, ball mills (Retsch, Ultra Turrax, Precellys, MP FastPrep), blade mills (Storaum) or sonicators (Bransonic, Bactosonic).
- the grinding operation requires the addition of a liquid medium in which the analytes (microorganisms, nucleic acids, proteins or other organic or inorganic compounds) will be transferred due to grinding.
- analytes microorganisms, nucleic acids, proteins or other organic or inorganic compounds
- the liquid medium is removed after grinding as would be a liquid sample and introduced into the usual chain of analysis (manual seeding, automatic seeding, chemical or biochemical analysis, gene amplification, immunoassay, chromatography, mass spectrometry or any other method of analysis. appropriate analysis).
- the sample is removed for transfer to the grinding device.
- the grinding medium is added if it was not already present in the grinding device, as well as the grinding balls if they were not present in the grinding device or in the grinding medium. Only then can grinding be done.
- This sample preparation involves several successive steps with repeated manipulations, and multiple processes and reagents whose traceability is difficult to establish. These successive steps can therefore lead to a degradation of the sample and possibly to its contamination by analytes that were not initially present in the sample. Thus, the information provided by the analyzes is neither sufficiently specific nor sufficiently reliable.
- This technique remains complex to implement. Moreover, it does not allow to perform analyzes before the operation of removal of the material or when the prosthesis remains in place after debridement.
- Some laboratories use devices comprising a container with a fin driving the mobilization of the grinding balls without shaking the bottle (for example marketed under the name UltraTurrax).
- the object of the invention is to overcome these drawbacks by proposing a device for the collection, pre-analytical processing, transport and grinding of solid samples, making it possible to limit as far as possible the manipulations of the sample between its sampling and its analysis under liquid form by an appropriate method and to ensure the traceability of the samples.
- the invention relates to a device for the collection, preanalytical treatment, transport and grinding of solid samples
- a device for the collection, preanalytical treatment, transport and grinding of solid samples comprising a bottle with an opening closed by a removable plug, intended to receive a solid sample and in which medium is present.
- preanalytical a plurality of balls of a solid material and a bead retaining means designed to become ineffective when the vial is mechanically agitated or closed by the stopper, such that when the vial is mechanically agitated, the impact of the beads causes the solid sample to be crushed and mixed with the preanalytical medium.
- This retaining means makes it possible to prevent the balls from escaping from the flask when the sample is introduced into it and before that the bottle is closed and therefore to be certain that the appropriate number of beads is present during agitation.
- the simplest preanalytical medium will be sterile purified water for osmotic lysis of eukaryotic cells. It may also be a microbiological transport medium of the AMIES type, a cell culture medium for the detection of viruses, a lysis buffer or nucleic acid stabilization (for example, in the case of ribonucleic acids, RNAlater marketed by Qiagen) or a buffer for the analysis of proteins.
- certain preanalytical media are composed of a plurality of solid and / or liquid components which must only be reconstituted at the moment of use of the device in order to preserve their properties.
- at least a portion of the preanalytical medium is isolated from the rest of the flask by a retaining means designed to become ineffective when the flask is mechanically agitated or closed by the stopper.
- These multicomponent preanalytical media will then be reconstituted by mixing the various components at the time of closure of the device by screwing the plug or at the time of grinding by stirring. It is also possible to provide a component which is retained in the bottle until the bottle is opened by unscrewing the stopper after the grinding operation.
- the means for retaining the balls and components of the preanalytical medium may be common or independent.
- this device When the device is used for microbiological analysis, it must be sterile. The sterility of the device is then ensured by the chemical or physical sterilization of the components of the device before or after their assembly.
- this device in the form of a completely sterile kit with which the only human intervention consists in opening the bottle to introduce the sample and then closing. The flask is then mechanically stirred and a sample is then introduced into the analytical chain as would be an initially liquid sample.
- This device thus prevents contamination or depletion of the sample, between its introduction into the bottle and its analysis.
- the balls are made of paramagnetic material and the retaining means comprises a removable magnet.
- the retaining means comprises a gel.
- the retaining means is made of a tearable or breakable material.
- the retaining means is a membrane fixed in the cap of the bottle, so as to provide a housing for the plurality of balls.
- the retaining means is a pocket containing the plurality of balls.
- the bag may also contain at least a portion of the preanalytical medium, the material constituting the membrane then being impervious to liquids.
- the various means for retaining beads and components of the preanalytical medium can be combined. They can be common or independent of each other.
- the preanalytical medium is preferably in the form of a liquid or a gel.
- the device according to the invention is advantageously disposable, especially when used in the medical field.
- FIG. 1 is a sectional view of a device according to the invention
- FIG. 2 is a sectional view of an alternative embodiment of the device according to the invention.
- FIG. 3 is a sectional view showing another variant of the device according to the invention.
- FIG. 4 is a sectional view showing a variant of the device illustrated in FIG. 3 and,
- FIG. 5 is a sectional view showing a variant of the device shown in Figure 4.
- FIG. 1 shows a bottle 1 whose opening 11 is closed by a stopper 2.
- the neck 10 of the bottle has a thread 12 which cooperates with a thread 21 provided on the skirt 20 of the cap.
- beads 3 and a preanalytical medium 4, here in liquid form are placed inside the bottle 1 .
- These beads can be made of glass or metal.
- the vial like the beads and the transport medium, are sterile.
- Figure 1 shows a magnet 5 which is here provided on the bottom of the bottle. This magnet is not systematically provided.
- the magnet makes it possible to immobilize the balls against the wall of the bottle, near the magnet.
- the balls may in particular be made of stainless steel AISI 404.
- the device according to the invention is used in the following manner, for example in an operating theater.
- a person opens the bottle, deposits a solid sample and closes it.
- the bottle can be shaken manually. It can also be placed in a suitable machine.
- the preanalytical medium could also be in the form of a gel, so as to retain the beads in the bottle before stirring, the gel becoming liquid after stirring.
- a weakly concentrated agarose gel (0.3% for example) fulfills these requirements.
- FIG. 2 illustrates an alternative embodiment, in which the balls 3 are placed in a housing 24 fixed to the upper part 22 of the plug 2.
- This housing is closed by a membrane 23 made of a tearable material, for example a thin film of polystyrene or another suitable polymer.
- the bottle 1 always comprises a preanalytical medium 4.
- the device is used as before.
- the membrane 23 breaks and the balls 3 can fulfill their grinding function.
- the ball retaining means may be a housing formed in the plug which opens when screwed onto the neck 10 of the bottle.
- a lid may be torn by screwing the cap, or a second plug with the opposite screw thread may open the housing during screwing of the cap.
- Figure 3 shows a variant of the device in which the balls 3 are disposed inside a pocket 6, itself made of a tearable material for example a thin film of polystyrene or another suitable polymer.
- This pocket is here placed in the preanalytical medium 4. It should therefore be made of a material impervious to liquids. Other solutions could be envisaged, for example the attachment of the pocket 6 to the stopper 2.
- the device is used as before.
- the bag 6 breaks and the beads are mixed with the sample and the preanalytical medium 4. They can thus fulfill their grinding function.
- FIG. 4 illustrates another variant embodiment in which both the preanalytical medium 4 and the balls 3 are placed inside a pocket 7.
- the pocket 7 is made of a tearable and liquid impervious material for example a thin film of polystyrene or another suitable polymer.
- the bag 7 breaks to release both the preanalytical medium 4 and the beads 3. These can thus fulfill their grinding function.
- the preanalytical medium and the beads are placed in the bottom of the flask and they are separated from the remainder of the flask by a paraffin operculum or a breakable polymer.
- This seal makes it possible to separate the preanalytical medium and the beads from the sample, until crushing.
- the preanalytical medium 4 is directly poured into the flask.
- preanalytical media activated by the mixture of several active components stored separately can be provided. This is particularly the case for components whose long-term stability requires the separation of two or more liquid components, or the separation of one or more solid components and one or more liquid components. These components may for example be provided in housings closed by tear-off or breakable lids by the balls during agitation or by screwing the cap after introduction of the sample.
- One of the components may also be provided in a housing in which it will be retained until the opening of the bottle and therefore once the grinding operation is complete.
- the retaining means of the preanalytical medium components may be combined with the other bead retaining means.
- a gel or a magnet retaining the beads may be associated with a tear-off seal retaining a liquid and / or containing a powder.
- FIG. 5 illustrates a variant of the embodiment according to FIG. 4.
- a component 41 of the preanalytical medium is provided in the pocket 7 which also contains balls 3.
- Another pocket 8 contains two other components 42 and 43 of the preanalytical medium.
- the component 42 is in liquid form and the pocket 8 is therefore waterproof. It can be made of a tearable polymer film. This can be designed to release a grinding additive.
- the component 43 is in solid form (compressed powder for example).
- a component 44 of the preanalytical medium may be provided in a housing 24 provided in the cap 2 which is closed by a tear-off lid 25. This opens when the bottle is agitated (see FIG. 2) or under the effect screwing the plug.
- the device according to the invention makes it possible to limit the human interventions between the sampling of the solid sample and the subsequent analysis. It thus makes it possible to increase the reliability of the analysis and the traceability of the analytical process.
- the device When used in the medical field, the device is preferably disposable.
Landscapes
- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Food Science & Technology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
Description
Claims
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR1255101A FR2991305B1 (en) | 2012-06-01 | 2012-06-01 | DEVICE FOR THE COLLECTION, PREANALYTIC TREATMENT, TRANSPORT AND MILLING OF SOLID SAMPLES. |
PCT/IB2013/054423 WO2013179232A1 (en) | 2012-06-01 | 2013-05-29 | Device for the collection, pre-analytic treatment, transport and grinding of solid samples |
Publications (2)
Publication Number | Publication Date |
---|---|
EP2855296A1 true EP2855296A1 (en) | 2015-04-08 |
EP2855296B1 EP2855296B1 (en) | 2018-07-04 |
Family
ID=46852151
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP13737408.8A Active EP2855296B1 (en) | 2012-06-01 | 2013-05-29 | Device for the collection, pre-analytic treatment, transport and grinding of solid samples |
Country Status (6)
Country | Link |
---|---|
US (1) | US10611522B2 (en) |
EP (1) | EP2855296B1 (en) |
DK (1) | DK2855296T3 (en) |
ES (1) | ES2689811T3 (en) |
FR (1) | FR2991305B1 (en) |
WO (1) | WO2013179232A1 (en) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2991305B1 (en) * | 2012-06-01 | 2015-05-01 | Assist Publ Hopitaux De Paris | DEVICE FOR THE COLLECTION, PREANALYTIC TREATMENT, TRANSPORT AND MILLING OF SOLID SAMPLES. |
DK3482821T3 (en) * | 2017-11-09 | 2021-05-03 | Visco Jet Ruehrsysteme Gmbh | STIRS |
CN108579478A (en) * | 2018-05-10 | 2018-09-28 | 重庆市永川区天堂化工厂 | Pesticide extraction element for pesticide producing |
FR3081298B1 (en) * | 2018-05-23 | 2022-12-23 | Oreal | DEVICE FOR PREPARING A COSMETIC COMPOSITION, SET OF CAPSULES AND ASSOCIATED METHOD FOR PREPARING |
EP3837524B1 (en) * | 2018-08-13 | 2023-10-04 | Accroma Labtec AG | Automated preparation of sample materials for chemical analysis |
US11912480B2 (en) * | 2018-12-12 | 2024-02-27 | Katie Grobman | Container cap for controlled mixing and dispensing |
CN112657395A (en) * | 2021-01-19 | 2021-04-16 | 广东海洋大学 | Test tube vibration device for laboratory analysis test |
Family Cites Families (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3894692A (en) * | 1974-05-23 | 1975-07-15 | Leonid Yakovlevich Sanko | Process and apparatus for the preparation of asbestos cement suspension |
JPS63256122A (en) * | 1987-04-13 | 1988-10-24 | Nisshinbo Ind Inc | Method for agitating, mixing and injecting high-viscosity liquid |
US6786330B2 (en) * | 1997-10-14 | 2004-09-07 | Biogaia Ab | Two-compartment container |
CA2436574A1 (en) * | 2000-12-06 | 2002-06-13 | Pharmacia Corporation | Laboratory scale milling process |
US20020118595A1 (en) * | 2001-02-26 | 2002-08-29 | Miller Scott H. | Enclosed implantable material mixing system |
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2012
- 2012-06-01 FR FR1255101A patent/FR2991305B1/en active Active
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2013
- 2013-05-29 US US14/404,145 patent/US10611522B2/en active Active
- 2013-05-29 EP EP13737408.8A patent/EP2855296B1/en active Active
- 2013-05-29 WO PCT/IB2013/054423 patent/WO2013179232A1/en active Application Filing
- 2013-05-29 ES ES13737408.8T patent/ES2689811T3/en active Active
- 2013-05-29 DK DK13737408.8T patent/DK2855296T3/en active
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US20150191276A1 (en) | 2015-07-09 |
WO2013179232A1 (en) | 2013-12-05 |
EP2855296B1 (en) | 2018-07-04 |
DK2855296T3 (en) | 2018-10-22 |
FR2991305B1 (en) | 2015-05-01 |
US10611522B2 (en) | 2020-04-07 |
ES2689811T3 (en) | 2018-11-15 |
FR2991305A1 (en) | 2013-12-06 |
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