EP2831283A4 - Procédés et composition permettant le séquençage d'acides nucléiques modifiés - Google Patents

Procédés et composition permettant le séquençage d'acides nucléiques modifiés

Info

Publication number
EP2831283A4
EP2831283A4 EP13769322.2A EP13769322A EP2831283A4 EP 2831283 A4 EP2831283 A4 EP 2831283A4 EP 13769322 A EP13769322 A EP 13769322A EP 2831283 A4 EP2831283 A4 EP 2831283A4
Authority
EP
European Patent Office
Prior art keywords
composition
methods
nucleic acids
modified nucleic
sequencing modified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP13769322.2A
Other languages
German (de)
English (en)
Other versions
EP2831283A1 (fr
Inventor
Jonas Korlach
Stephen Turner
Tyson A Clark
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pacific Biosciences of California Inc
Original Assignee
Pacific Biosciences of California Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pacific Biosciences of California Inc filed Critical Pacific Biosciences of California Inc
Publication of EP2831283A1 publication Critical patent/EP2831283A1/fr
Publication of EP2831283A4 publication Critical patent/EP2831283A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A90/00—Technologies having an indirect contribution to adaptation to climate change
    • Y02A90/10—Information and communication technologies [ICT] supporting adaptation to climate change, e.g. for weather forecasting or climate simulation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP13769322.2A 2012-03-30 2013-03-18 Procédés et composition permettant le séquençage d'acides nucléiques modifiés Withdrawn EP2831283A4 (fr)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201261617999P 2012-03-30 2012-03-30
US201261721206P 2012-11-01 2012-11-01
US201261721339P 2012-11-01 2012-11-01
US201361799237P 2013-03-15 2013-03-15
US201361789354P 2013-03-15 2013-03-15
PCT/US2013/032816 WO2013148400A1 (fr) 2012-03-30 2013-03-18 Procédés et composition permettant le séquençage d'acides nucléiques modifiés

Publications (2)

Publication Number Publication Date
EP2831283A1 EP2831283A1 (fr) 2015-02-04
EP2831283A4 true EP2831283A4 (fr) 2015-11-04

Family

ID=49261113

Family Applications (1)

Application Number Title Priority Date Filing Date
EP13769322.2A Withdrawn EP2831283A4 (fr) 2012-03-30 2013-03-18 Procédés et composition permettant le séquençage d'acides nucléiques modifiés

Country Status (4)

Country Link
EP (1) EP2831283A4 (fr)
AU (1) AU2013240166A1 (fr)
CA (1) CA2867489A1 (fr)
WO (1) WO2013148400A1 (fr)

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US9290800B2 (en) 2013-03-15 2016-03-22 Pacific Biosciences Of California, Inc. Targeted rolling circle amplification
EP3974814A1 (fr) * 2013-11-17 2022-03-30 Quantum-si Incorporated Dispositif intégré doté d'une source de lumière externe pour sondage, détection et d'analyse de molécules
CN107109460A (zh) * 2014-10-22 2017-08-29 艾比斯生物科学公司 细菌表观基因组分析
US9618474B2 (en) 2014-12-18 2017-04-11 Edico Genome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10006910B2 (en) 2014-12-18 2018-06-26 Agilome, Inc. Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same
US9857328B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same
US10020300B2 (en) 2014-12-18 2018-07-10 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
EP3235010A4 (fr) 2014-12-18 2018-08-29 Agilome, Inc. Transistor à effet de champ chimiquement sensible
US9859394B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
WO2017201081A1 (fr) 2016-05-16 2017-11-23 Agilome, Inc. Dispositifs à fet au graphène, systèmes et leurs méthodes d'utilisation pour le séquençage d'acides nucléiques
MX2018014856A (es) * 2016-06-01 2019-03-07 Quantum Si Inc Llamador de pulso y llamador de base.
EP3366780B1 (fr) * 2017-02-23 2020-05-06 Siemens Healthcare GmbH Analyse de séquence de molécules individuelles et de méthylation à très haute sensibilité pour analyse spécifique de tissus
CN111542616A (zh) * 2017-11-03 2020-08-14 夸登特健康公司 脱氨引起的序列错误的纠正
TWI812671B (zh) 2018-01-26 2023-08-21 美商寬騰矽公司 用於識別核酸之核苷酸之方法、系統及非暫時性電腦可讀儲存媒體以及用於訓練深度學習模型之方法及系統
CN112534063A (zh) 2018-05-22 2021-03-19 安序源有限公司 用于核酸测序的方法、系统和组合物
WO2023177771A1 (fr) * 2022-03-17 2023-09-21 Regeneron Pharmaceuticals, Inc. Procédés de caractérisation de génome viral à l'aide de modifications de bases

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WO2008154317A1 (fr) * 2007-06-06 2008-12-18 Pacific Biosciences Of California, Inc. Méthodes et procédés pour identifier des bases dans des procédés d'incorporation en fonction de la séquence
WO2009145818A1 (fr) * 2008-03-31 2009-12-03 Pacific Biosciences Of California, Inc Procédés et compositions de charge de molécule individuelle
WO2010068289A2 (fr) * 2008-12-11 2010-06-17 Pacific Biosciences Of California, Inc. Classification de matrices d'acides nucléiques
US20110183320A1 (en) * 2008-12-11 2011-07-28 Pacific Biosciences Of California, Inc. Classification of nucleic acid templates

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EP0711303B2 (fr) 1993-07-30 2009-06-10 Affymax, Inc. Biotinylation de proteines
US5795782A (en) 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
EP1105529B2 (fr) 1998-07-30 2013-05-29 Illumina Cambridge Limited Biomolecules en rangees et leur utilisation dans une procedure de sequencage
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6917726B2 (en) 2001-09-27 2005-07-12 Cornell Research Foundation, Inc. Zero-mode clad waveguides for performing spectroscopy with confined effective observation volumes
US7670405B2 (en) 2003-08-06 2010-03-02 Department Of Science & Technology (Dst) Process for the manufacture of a bio-release fertilizer of an anionic micro nutrient viz molybdenum
US20060046258A1 (en) * 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
US7399614B2 (en) 2004-06-30 2008-07-15 Applera Corporation 5-methylcytosine detection, compositions and methods therefor
US7315019B2 (en) 2004-09-17 2008-01-01 Pacific Biosciences Of California, Inc. Arrays of optical confinements and uses thereof
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
US7805081B2 (en) 2005-08-11 2010-09-28 Pacific Biosciences Of California, Inc. Methods and systems for monitoring multiple optical signals from a single source
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
EP1938099A4 (fr) 2005-09-30 2009-11-11 Pacific Biosciences California Surfaces réactives, substrats et procédés de production et d'utilisation correspondants
US7998717B2 (en) 2005-12-02 2011-08-16 Pacific Biosciences Of California, Inc. Mitigation of photodamage in analytical reactions
CA2633524A1 (fr) 2005-12-22 2007-07-05 Pacific Biosciences Of California, Inc. Polymerases permettant d'incorporer des analogues de nucleotides
WO2008028160A2 (fr) 2006-09-01 2008-03-06 Pacific Biosciences Of California, Inc. Substrats, systèmes et procédés d'analyse de matériaux
US20080277595A1 (en) 2007-05-10 2008-11-13 Pacific Biosciences Of California, Inc. Highly multiplexed confocal detection systems and methods of using same
US7901889B2 (en) 2007-07-26 2011-03-08 Pacific Biosciences Of California, Inc. Molecular redundant sequencing
EP2255014A4 (fr) 2008-02-12 2011-05-18 Pacific Biosciences California Compositions et procédés utilisables dans des réactions analytiques
US8652781B2 (en) 2008-02-12 2014-02-18 Pacific Biosciences Of California, Inc. Cognate sampling kinetics
EP2263087B1 (fr) 2008-03-13 2017-08-09 Pacific Biosciences of California, Inc. Réactifs marqués et leurs utilisations
US7973146B2 (en) 2008-03-26 2011-07-05 Pacific Biosciences Of California, Inc. Engineered fluorescent dye labeled nucleotide analogs for DNA sequencing
US8236499B2 (en) 2008-03-28 2012-08-07 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
CA2991818C (fr) 2008-03-28 2022-10-11 Pacific Biosciences Of California, Inc. Compositions et procedes pour le sequencage d'acide nucleique
AU2009251883B2 (en) 2008-03-31 2014-09-11 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
DK3629011T3 (da) 2008-09-16 2024-01-29 Pacific Biosciences California Inc Integreret optisk indretning
US8481264B2 (en) 2008-09-19 2013-07-09 Pacific Biosciences Of California, Inc. Immobilized nucleic acid complexes for sequence analysis
WO2010036287A1 (fr) 2008-09-24 2010-04-01 Pacific Biosciences Of California, Inc. Détection intermittente durant des réactions analytiques
US8370079B2 (en) 2008-11-20 2013-02-05 Pacific Biosciences Of California, Inc. Algorithms for sequence determination
JP5439822B2 (ja) 2009-01-15 2014-03-12 独立行政法人物質・材料研究機構 Ｎｉ基単結晶超合金
US9017937B1 (en) 2009-04-10 2015-04-28 Pacific Biosciences Of California, Inc. Nanopore sequencing using ratiometric impedance
EP2425023B1 (fr) 2009-04-27 2015-12-23 Pacific Biosciences of California, Inc. Procédés et systèmes de séquençage en temps réel
WO2011103421A1 (fr) * 2010-02-18 2011-08-25 Shuber Anthony P Compositions et méthodes pour le traitement du cancer
US20130138358A1 (en) 2010-02-24 2013-05-30 Pacific Biosciences Of California, Inc. Algorithms for sequence determination
US9165109B2 (en) 2010-02-24 2015-10-20 Pacific Biosciences Of California, Inc. Sequence assembly and consensus sequence determination

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008154317A1 (fr) * 2007-06-06 2008-12-18 Pacific Biosciences Of California, Inc. Méthodes et procédés pour identifier des bases dans des procédés d'incorporation en fonction de la séquence
WO2009145818A1 (fr) * 2008-03-31 2009-12-03 Pacific Biosciences Of California, Inc Procédés et compositions de charge de molécule individuelle
WO2010068289A2 (fr) * 2008-12-11 2010-06-17 Pacific Biosciences Of California, Inc. Classification de matrices d'acides nucléiques
US20110183320A1 (en) * 2008-12-11 2011-07-28 Pacific Biosciences Of California, Inc. Classification of nucleic acid templates

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BENJAMIN FLUSBERG: "Direct detection of DNA methylation and mutagenic damage through single-molecule, real-time (SMRT (TM)) DNA sequencing", 101ST ANNUAL MEETING OF THE AMERICAN-ASSOCIATION-FOR-CANCER-RESEARCH; WASHINGTON, DC, USA; APRIL 17 -21, 2010, 1 April 2010 (2010-04-01), XP055215735, Retrieved from the Internet <URL:http://www.abstractsonline.com/Plan/ViewAbstract.aspx?mID=2521&sKey=ad61b071-5ab9-4f64-bf00-14f780960c07&cKey=68c67e8a-73b3-4b54-8a51-7bc1e5ae1748&mKey=0591fa3b-afef-49d2-8e65-55f41ee8117e> [retrieved on 20150923] *
FLUSBERG BENJAMIN A ET AL: "Direct detection of DNA methylation during single-molecule, real-time sequencing", NATURE METHODS, NATURE PUBLISHING GROUP, GB, vol. 7, no. 6, 1 July 2010 (2010-07-01), pages 461 - 465, XP009142171, ISSN: 1548-7105, [retrieved on 20100509], DOI: 10.1038/NMETH.1459 *
See also references of WO2013148400A1 *
THOMPSON JOHN F ET AL: "The properties and applications of single-molecule DNA sequencing", GENOME BIOLOGY, BIOMED CENTRAL LTD., LONDON, GB, vol. 12, no. 2, 24 February 2011 (2011-02-24), pages 217, XP021091788, ISSN: 1465-6906 *

Also Published As

Publication number Publication date
EP2831283A1 (fr) 2015-02-04
AU2013240166A1 (en) 2014-10-30
WO2013148400A1 (fr) 2013-10-03
CA2867489A1 (fr) 2013-10-03

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