EP2794872A4 - Polypeptides présentant une activité de bêta-glucosidase et polynucléotides codant pour ceux-ci - Google Patents

Polypeptides présentant une activité de bêta-glucosidase et polynucléotides codant pour ceux-ci

Info

Publication number
EP2794872A4
EP2794872A4 EP12860010.3A EP12860010A EP2794872A4 EP 2794872 A4 EP2794872 A4 EP 2794872A4 EP 12860010 A EP12860010 A EP 12860010A EP 2794872 A4 EP2794872 A4 EP 2794872A4
Authority
EP
European Patent Office
Prior art keywords
polypeptides
beta
polynucleotides encoding
glucosidase activity
encoding same
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP12860010.3A
Other languages
German (de)
English (en)
Other versions
EP2794872A1 (fr
Inventor
Junxin Duan
Yu Zhang
Lan Tang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes Inc
Original Assignee
Novozymes Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Inc filed Critical Novozymes Inc
Publication of EP2794872A1 publication Critical patent/EP2794872A1/fr
Publication of EP2794872A4 publication Critical patent/EP2794872A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C12N15/8246—Non-starch polysaccharides, e.g. cellulose, fructans, levans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
EP12860010.3A 2011-12-19 2012-12-19 Polypeptides présentant une activité de bêta-glucosidase et polynucléotides codant pour ceux-ci Withdrawn EP2794872A4 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN2011084228 2011-12-19
PCT/CN2012/086938 WO2013091544A1 (fr) 2011-12-19 2012-12-19 Polypeptides présentant une activité de bêta-glucosidase et polynucléotides codant pour ceux-ci

Publications (2)

Publication Number Publication Date
EP2794872A1 EP2794872A1 (fr) 2014-10-29
EP2794872A4 true EP2794872A4 (fr) 2015-12-09

Family

ID=48667711

Family Applications (1)

Application Number Title Priority Date Filing Date
EP12860010.3A Withdrawn EP2794872A4 (fr) 2011-12-19 2012-12-19 Polypeptides présentant une activité de bêta-glucosidase et polynucléotides codant pour ceux-ci

Country Status (4)

Country Link
US (1) US9771568B2 (fr)
EP (1) EP2794872A4 (fr)
BR (1) BR112014014760A2 (fr)
WO (1) WO2013091544A1 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014060379A1 (fr) * 2012-10-16 2014-04-24 Dsm Ip Assets B.V. Enzymes de dégradation de la paroi cellulaire tirées de myceliophthora fergusii (corynascus thermophilus) et leurs utilisations
EP2824179B1 (fr) * 2013-07-09 2017-11-29 Honda Motor Co., Ltd. Bêta-glucosidase
EP2824178B1 (fr) * 2013-07-09 2017-12-06 Honda Motor Co., Ltd. Bêta-glucosidase
EP2826858B1 (fr) * 2013-07-09 2017-05-31 Honda Motor Co., Ltd. Bêta-glucosidase
US9228181B2 (en) 2013-07-09 2016-01-05 Honda Motor Co., Ltd. β-glucosidase
KR20180012254A (ko) 2015-04-10 2018-02-05 코멧 바이오리파이닝 인코포레이티드 셀룰로스 바이오매스의 처리를 위한 방법 및 조성물 및 이에 의해 생성된 생성물
CN108883400B (zh) 2016-02-19 2021-09-17 洲际大品牌有限责任公司 由生物质源形成多值料流的方法
CN109385411B (zh) * 2017-08-04 2021-10-26 东莞泛亚太生物科技有限公司 一种β-甘露糖苷酶及其应用
CN107365780A (zh) * 2017-08-10 2017-11-21 广东省生物资源应用研究所 一种黄胸散白蚁唾液腺β‑糖苷酶基因的克隆及原核表达方法
EA202092695A1 (ru) 2018-05-10 2021-02-25 Комет Байорифайнинг Инк. Композиции, содержащие глюкозу и гемицеллюлозу, и их применение
CA3143527A1 (fr) 2019-08-06 2021-02-11 Novozymes A/S Proteines de fusion pour une expression enzymatique amelioree

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011066457A2 (fr) * 2009-11-25 2011-06-03 Codexis, Inc. Variants recombinés de la béta-glucosidase destinés à produire des sucres solubles à partir d'une biomasse cellulosique
WO2014059541A1 (fr) * 2012-10-16 2014-04-24 Concordia University Nouvelles enzymes de déconstruction de la paroi cellulaire de thermoascus aurantiacus, myceliophthora fergusii (corynascus thermophilus) et pseudocercosporella herpotrichoides et leurs utilisations
WO2014060379A1 (fr) * 2012-10-16 2014-04-24 Dsm Ip Assets B.V. Enzymes de dégradation de la paroi cellulaire tirées de myceliophthora fergusii (corynascus thermophilus) et leurs utilisations

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009073723A1 (fr) * 2007-12-06 2009-06-11 Novozymes A/S Polypeptides ayant une activité bêta-glucosidase et polynucléotides les codant
CN102712916B (zh) 2009-09-18 2015-11-25 诺维信股份有限公司 具有β-葡糖苷酶活性的多肽和编码该多肽的多核苷酸

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011066457A2 (fr) * 2009-11-25 2011-06-03 Codexis, Inc. Variants recombinés de la béta-glucosidase destinés à produire des sucres solubles à partir d'une biomasse cellulosique
WO2014059541A1 (fr) * 2012-10-16 2014-04-24 Concordia University Nouvelles enzymes de déconstruction de la paroi cellulaire de thermoascus aurantiacus, myceliophthora fergusii (corynascus thermophilus) et pseudocercosporella herpotrichoides et leurs utilisations
WO2014060379A1 (fr) * 2012-10-16 2014-04-24 Dsm Ip Assets B.V. Enzymes de dégradation de la paroi cellulaire tirées de myceliophthora fergusii (corynascus thermophilus) et leurs utilisations

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
COLLINS ET AL: "Molecular cloning and expression analysis of two distinct beta-glucosidase genes, bg1 and aven1, with very different biological roles from the thermophilic, saprophytic fungus Talaromyces emersonii", MYCOLOGICAL RESEARCH, ELSEVIER, GB, vol. 111, no. 7, 6 August 2007 (2007-08-06), pages 840 - 849, XP022188075, ISSN: 0953-7562, DOI: 10.1016/J.MYCRES.2007.05.007 *
KRISTIAN B R M KROGH ET AL: "Characterization and kinetic analysis of a thermostable GH3 beta-glucosidase from Penicillium brasilianum", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER, BERLIN, DE, vol. 86, no. 1, 16 September 2009 (2009-09-16), pages 143 - 154, XP019799835, ISSN: 1432-0614 *
LEONTINA GURGU ET AL: "Fermentation of cellobiose to ethanol by industrial strains carrying the beta-glucosidase gene (BGL1) from Saccharomyces fibuligera", BIORESOURCE TECHNOLOGY, ELSEVIER BV, GB, vol. 102, no. 8, 19 January 2011 (2011-01-19), pages 5229 - 5236, XP028162740, ISSN: 0960-8524, [retrieved on 20110125], DOI: 10.1016/J.BIORTECH.2011.01.062 *
RAJ KUMAR ET AL: "Bioconversion of lignocellulosic biomass: biochemical and molecular perspectives", JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY ; OFFICIAL JOURNAL OF THE SOCIETY FOR INDUSTRIAL MICROBIOLOGY, SPRINGER, BERLIN, DE, vol. 35, no. 5, 13 March 2008 (2008-03-13), pages 377 - 391, XP019596328, ISSN: 1476-5535 *
ROSGAARD LISA ET AL: "Efficiency of new fungal cellulase systems in boosting enzymatic degradation of barley straw lignocellulose", BIOTECHNOLOGY PROGRESS, AMERICAN INSTITUTE OF CHEMICAL ENGINEERS, US, vol. 22, no. 2, 1 March 2006 (2006-03-01), pages 493 - 498, XP002565690, ISSN: 8756-7938, [retrieved on 20060324], DOI: 10.1021/BP050361O *
SAUMITA BANERJEE ET AL: "Commercializing lignocellulosic bioethanol: technology bottlenecks and possible remedies", BIOFUELS, BIOPRODUCTS AND BIOREFINING, vol. 4, no. 1, 1 January 2010 (2010-01-01), pages 77 - 93, XP055200685, ISSN: 1932-104X, DOI: 10.1002/bbb.188 *
See also references of WO2013091544A1 *

Also Published As

Publication number Publication date
BR112014014760A2 (pt) 2017-06-13
US9771568B2 (en) 2017-09-26
US20140331364A1 (en) 2014-11-06
EP2794872A1 (fr) 2014-10-29
WO2013091544A1 (fr) 2013-06-27

Similar Documents

Publication Publication Date Title
ZA201403761B (en) Polypeptides having lysozyme activity and polynucleotides encoding same
HRP20180854T1 (hr) Polipeptid koji ima aktivnost beta-glukozidaze i njegova uporaba
IL262667B (en) An isolated polynucleotide encoding a polypeptide and a pharmaceutical composition containing the isolated polynucleotide
EP2678352A4 (fr) Polypeptides permettant de faciliter l'activité cellulolytique et polynucléotides codant pour de tels polypeptides
EP2702071A4 (fr) Polypeptides capables de favoriser l'activité cellulolytique et polynucléotides codant pour ceux-ci
EP2638153A4 (fr) Polypeptides ayant une activité endoglucanase et polynucléotides codant pour ceux-ci
HK1207665A1 (en) Polypeptides having transgalactosylating activity
EP2794872A4 (fr) Polypeptides présentant une activité de bêta-glucosidase et polynucléotides codant pour ceux-ci
HUE039835T2 (hu) Alfa-amiláz variánsok és az azokat kódoló polinukleotidok
PL2588492T3 (pl) Polipeptyd mający aktywność beta-glukozydazy i jego zastosowania
IL229258A0 (en) polypeptides
EP2748188A4 (fr) Polypeptides ayant une activité glucoamylase et polynucléotides codant pour ces polypeptides
EP2635594A4 (fr) Polypeptides présentant une activité de cellobiohydrolase et polynucléotides codant pour ceux-ci
EP2791330A4 (fr) Polypeptides ayant une activité laccase et polynucléotides les codant
EP2751131A4 (fr) Polypeptides à activité alpha-amylase et polynucléotides codant pour ceux-ci
EP2748189A4 (fr) Polypeptides ayant une activité alpha-amylase et polynucléotides codant pour ceux-ci
EP2931744A4 (fr) Polypeptides ayant une activité phospholipase c et polynucléotides codant pour ceux-ci
EP2785732A4 (fr) Polypeptides à activité bêta-xylosidase et polynucléotides codant pour ceux-ci
EP2782998A4 (fr) Polypeptides ayant une activité bêta-xylosidase et polynucléotides codant pour ceux-ci
EP2935574A4 (fr) Alpha-amylases et polynucléotides les codant
EP2794664A4 (fr) Polypeptides hybrides ayant une activité de cellobiohydrolase et polynucléotides codant pour lesdits polypeptides
SG11201402139UA (en) High mast2-affinity polypeptides and uses thereof
EP3060666A4 (fr) Polypeptides présentant une activité endoglucanase et polynucléotides codant pour ceux-ci
EP2780362A4 (fr) Polypeptides ayant une activité d'endoglucanase et polynucléotides codant pour lesdits polypeptides
EP2773656A4 (fr) Polypeptides présentant une activité cellulolytique augmentée et polynucléotides codant pour ceux-ci

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20140721

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

DAX Request for extension of the european patent (deleted)
RIC1 Information provided on ipc code assigned before grant

Ipc: C12P 19/14 20060101ALI20150716BHEP

Ipc: C12N 15/52 20060101ALI20150716BHEP

Ipc: A01H 1/00 20060101ALI20150716BHEP

Ipc: C12N 9/42 20060101AFI20150716BHEP

RA4 Supplementary search report drawn up and despatched (corrected)

Effective date: 20151109

RIC1 Information provided on ipc code assigned before grant

Ipc: C12P 19/14 20060101ALI20151103BHEP

Ipc: C12N 15/52 20060101ALI20151103BHEP

Ipc: A01H 1/00 20060101ALI20151103BHEP

Ipc: C12N 9/42 20060101AFI20151103BHEP

17Q First examination report despatched

Effective date: 20161104

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20170516