EP2743390A1 - Method and device for production of nanofibrous textile, mainly for seeding living organisms - Google Patents

Method and device for production of nanofibrous textile, mainly for seeding living organisms Download PDF

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Publication number
EP2743390A1
EP2743390A1 EP13193616.3A EP13193616A EP2743390A1 EP 2743390 A1 EP2743390 A1 EP 2743390A1 EP 13193616 A EP13193616 A EP 13193616A EP 2743390 A1 EP2743390 A1 EP 2743390A1
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EP
European Patent Office
Prior art keywords
nanofibers
gas
spinning
collecting electrode
production
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP13193616.3A
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German (de)
English (en)
French (fr)
Inventor
Jan Cmelík
Ladislav Sevcík
David Vejrych
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Technicka Univerzita v Liberci
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Technicka Univerzita v Liberci
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Publication date
Application filed by Technicka Univerzita v Liberci filed Critical Technicka Univerzita v Liberci
Publication of EP2743390A1 publication Critical patent/EP2743390A1/en
Withdrawn legal-status Critical Current

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Classifications

    • D—TEXTILES; PAPER
    • D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
    • D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
    • D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
    • D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
    • D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
    • D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
    • D01D5/00—Formation of filaments, threads, or the like
    • D01D5/0007—Electro-spinning
    • D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
    • D01D5/0076—Electro-spinning characterised by the electro-spinning apparatus characterised by the collecting device, e.g. drum, wheel, endless belt, plate or grid

Definitions

  • the invention relates to a method of production of a nanofibrous textile, mainly for seeding living organisms and/or cells, for example for scaffolds in tissue engineering, using electrostatic spinning of polymers in an electrostatic field of high intensity.
  • the invention also relates to a device for production of a nanofibrous textile, mainly for seeding living organisms and/or cells, for example for scaffolds in tissue engineering, using electrostatic spinning of polymers in an electrostatic field of high intensity between a spinning electrode and a collecting electrode.
  • Tissue engineering is a branch of biomedicine, which deals primarily with substitution and regeneration of damaged tissues.
  • porous biodegradable matrices are used. Scaffolds are seeded with a cell culture and implemented into the area of damage. The cells seeded in scaffolds gradually proliferate in its porous structure and form a new tissue. The material from which a scaffold is made is subject to biological degradation in the organism and gradually gives way to newly formed tissue.
  • Scaffolds are created from biodegradable polymers using different methods, such as 3D printing or electrostatic spinning, whereby the method of electrostatic spinning seems to be very perspective for seeding with cells.
  • Conventionally produced nanofibrous textiles are formed by a relatively thin layer of nanofibers and have excellent properties for filtration, however, in relation to their total volume they have a small proportion of interfibrous spaces, therefore it is difficult to seed them with cells, or, in other words, they can be placed a smaller amount of cells than would be optimal for tissue engineering applications.
  • Seeding living organisms in textile carriers is also used in other fields, for example in fermentation processes or in waste water treatment plants.
  • nanofibrous textiles produced by electrostatic spinning of polymers are considered to be very perspective, whereby it appears that apart from the price the major disadvantage is a low proportion of interfibrous spaces in relation to the total volume of a nanofibrous textile.
  • the aim of the invention is therefore to propose a method for production of porous nanofibrous matrices or textiles with sufficient portion of interfibrous spaces and to create a device for production of such matrices, whereby for some applications the electrospun polymer must be biodegradable.
  • the goal of the invention is achieved through a method of spinning of biodegradable polymers, whose principle consists in that during depositing nanofibers into a layer by the action of electrostatic forces, nanofibers are acted upon by a stream of gas moving against the direction of the movement of nanofibers, by which means in the formed layer of nanofibers the interfibrous spaces are increased.
  • a space for seeding a greater amount of living organisms and/or cells is created.
  • discontinuous production a plurality of gas streams act against the direction of the movement of nanofibers within the whole deposited surface.
  • Discontinuous production is suitable mainly for production of small-size nanofibrous formations, especially scaffolds.
  • a textile produced in this manner is suitable for seeding for example with yeasts to be used in fermentation processes or for seeding with bacteria to be used in waste water treatment plants as well as for other processes, in which it is necessary to seed a large amount of living organisms and/or cells into a nanofibrous layer.
  • the gas to be supplied is inert gas.
  • the principle of the device according to the invention consists in that to a collecting electrode is aligned at least one jet for supplying the gas against the direction of the movement of the nanofibers.
  • FIG. 1 shows a device for discontinuous production
  • Fig. 2 shows a device for continuous production
  • Fig. 3 shows a longitudinal section of the collecting electrode according to Fig. 3
  • Fig. 4 is a side view of an embodiment of the collecting electrode for discontinuous production
  • Fig. 5 is an axonometric view of the collecting electrode according to Fig. 4 .
  • spinning electrodes 2 which are formed by a suitable known spinning electrode, for example by a cord, a rod, a row of tips or jets arranged next to each other, and are connected to one pole of a high voltage source 3.
  • the number of spinning electrodes 2 and their type serves merely as an example and depends on technological requirements. Person skilled in the art will select one using his experiences and, as the case may be, also according to the results of testing.
  • a collecting electrode 4 Arranged against the spinning electrodes 2 is a collecting electrode 4, which is connected to the other pole of the high voltage source 3 and in the illustrated embodiment is composed of a hollow plate, whose cavity constitutes a pressure chamber 41 , which is connected to a known unillustrated source of compressed gas and in whose wall oriented towards the spinning electrodes 2 are created jets 42 for the formation of streams 43 of gas directed against the spinning electrodes 2.
  • a support 5 Arranged between the spinning electrodes 2 and the collecting electrode 4 is a support 5 made of a gas permeable material, for example of a textile grid, a metal grid or a non-metal grid.
  • the gas used for creating streams 43 of gas may be according to the technological requirements air, inert gas or some other gas.
  • an electrostatic field of high intensity is created, which is able to create nanofibers 211 from the surface of the polymer 21 situated on the spinning electrode 2, and to carry them to the collecting electrode 4 and deposit them on the support 5.
  • the streams 43 of gas coming out of the jets 42 begin to act upon the nanofibres 211 .
  • the streams 43 of gas act upon the nanofibers 211 before their falling on the support 5 and slow down their flight, accelerate drying of solvents, thus increase the mechanical stiffness of the nanofibers 211 .
  • the streams 43 of gas act against the direction of the attraction force of the electrostatic forces acting between the nanofibers, and between the nanofibers and the collecting electrode 4 , and thereore the nanofibers are deposited in a layer with a larger volume and a larger amount of interfibrous spaces. If such a layer 2110 of nanofibers 211 is produced from a biodegradable polymer, it is very sufficient for usage in tissue engineering as a scaffold for seeding with cells.
  • the support 5 is made from electrically conductive material, it is advantageous if instead of the collecting electrode 4 the support 5 is connected to the other pole of the high voltage source 3.
  • the collecting electrode 4 is no voltage and it only serves to supply gas and create streams 43 of gas.
  • An electrostatic field of high intensity is then created between the spinning electrodes 2 and the support 5.
  • the collecting electrode 4 shown in Fig. 4 and Fig. 5 which comprises a flange 400 for mounting in the spinning chamber 1 of the device for production of nanofibers.
  • a cover plate 411 which is a part of the pressure chamber 41 .
  • the pressure chamber 41 is provided with inlets 412 of compressed air, by which it is in a known unillustrated manner connected to a source of the compressed air.
  • the second wall 413 of the pressure chamber 41 is fitted with a plurality of jets 42 for creating streams 43 of gas.
  • a frame 414 for fastening a metal grid 51 , which constitutes a support 5 and is connectable to the high voltage source 3 , so that if it is used during spinning, an electrostatic field of high intensity is created between the spinning electrodes 2 and the support 5 .
  • spinning electrodes 2 which are in the illustrated embodiment composed of cylindrical bodies carrying polymer out of a vessel into the spinning space on their surfaces, for example according to EP1673493 . It is possible to use spinning electrodes of any type, whereby, according to experiences, it is advisable to use nozzle-less spinning electrodes, in which spinning takes place from the surface of the polymer created on the surface of the body of the spinning electrode. Against the spinning electrodes 2 there are in the spinning chamber 1 arranged collecting electrodes 4.
  • an electrostatic field of high intensity for example by connecting each of the electrodes to a different pole of the high voltage source 3 , as is illustrated, or by connecting one of the electrodes to the high voltage source 3 and grounding the other electrode of the corresponding pair.
  • an electrostatic field of high intensity for example by connecting each of the electrodes to a different pole of the high voltage source 3 , as is illustrated, or by connecting one of the electrodes to the high voltage source 3 and grounding the other electrode of the corresponding pair.
  • the support 5 touches the surfaces of the collecting electrodes 4.
  • the length of the spinning electrodes 2, the length of the collecting electrodes 4 and the width of the support 5 correspond to the width of the created nanofibrous layer 2110 .
  • the collecting electrodes 4 are in the illustrated embodiment composed of a tube, whose cavity forms a pressure chamber 41 , which is closed on one side and on the other side is provided with an inlet 412 of compressed gas, through which it is in a known manner connected to a source of compressed gas.
  • the jets 42 are arranged in one row along the whole length or in more rows, for example in three rows, as in case of the illustrated embodiment.
  • Spinning takes place from the surface of the polymer situated on the surface of the spinning electrodes 2, which is according to the type of electrode renewed continuously or at certain intervals, and the nanofibers are carried by the influence of the electrostatic field towards the collecting electrode 4, from which streams 43 of gas are coming out against them, for example of air, which help to slow down their flight, accelerate vaporization of solvents and act against the direction of the attraction force of electrostatic forces, whereby interactions occur between the nanofibers 211 mutually as well as between the nanofibers 211 and the collecting electrode 4.
  • the nanofibers 211 therefore are deposited on the support 5 in a layer 2110 with a larger volume and a larger amount of interfibrous spaces than would be the case without the streams 43 of gas acting against their movement.
  • the produced nanofibrous textile is suitable especially for seeding living organisms and/or cells.
  • a method of production of nanofibrous textiles by electrostatic spinning of polymers in an electrostatic field of high intensity in which during deposition of nanofibers 211 into a layer 2110 by the action of the electrostatic forces there is acted by a stream 43 of gas against the direction of the movement of nanofibers 211 , by which means the interfibrous spaces in the formed layer 2110 of nanofibers 211 increase, as well as the volume increases, can be performed also on other devices than those described above.
  • the principle always consists in supplying streams of gas against the direction of the movement of nanofibers before they touch the support, which is always made from permeable material which gives little resistance to the penetrating gas.
  • the direction of spinning may vary, depending on particular known arrangements of spinning devices. Vertical spinning in upward direction has been chosen in the description only because its demonstration is usual and simple.

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  • Engineering & Computer Science (AREA)
  • Textile Engineering (AREA)
  • Mechanical Engineering (AREA)
  • Spinning Methods And Devices For Manufacturing Artificial Fibers (AREA)
  • Nonwoven Fabrics (AREA)
EP13193616.3A 2012-12-17 2013-11-20 Method and device for production of nanofibrous textile, mainly for seeding living organisms Withdrawn EP2743390A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CZ20120906A CZ304099B6 (cs) 2012-12-17 2012-12-17 Zpusob a zarízení k výrobe nanovlákenné textilie, zejména pro osazování zivými organizmy

Publications (1)

Publication Number Publication Date
EP2743390A1 true EP2743390A1 (en) 2014-06-18

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EP13193616.3A Withdrawn EP2743390A1 (en) 2012-12-17 2013-11-20 Method and device for production of nanofibrous textile, mainly for seeding living organisms

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EP (1) EP2743390A1 (cs)
CZ (1) CZ304099B6 (cs)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105586644A (zh) * 2015-12-10 2016-05-18 厦门大学 一种制备蓬松纳米纤维的电纺装置
CN106637439A (zh) * 2017-01-24 2017-05-10 厦门大学 自主式多喷头气泡静电纺丝装置
CN108754874A (zh) * 2018-06-22 2018-11-06 南通纺织丝绸产业技术研究院 大孔径纳米纤维膜的制备方法
CN108774809A (zh) * 2018-06-22 2018-11-09 南通纺织丝绸产业技术研究院 批量制备大孔径纳米纤维膜的方法
CN109457304A (zh) * 2018-12-06 2019-03-12 南通纺织丝绸产业技术研究院 一种制备大孔径纳米纤维的方法

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115467032B (zh) * 2022-08-22 2023-08-25 青岛大学 一种静电纺丝辅助装置

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0009941A1 (en) * 1978-10-10 1980-04-16 Imperial Chemical Industries Plc Production of electrostatically spun products
EP1673493A1 (en) 2003-09-08 2006-06-28 Technicka Univerzita v Liberci A method of nanofibres production from a polymer solution using electrostatic spinning and a device for carrying out the method
WO2012006072A2 (en) * 2010-06-28 2012-01-12 Virginia Commonwealth University Air impedance electrospinning for controlled porosity

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8808608B2 (en) * 2004-12-27 2014-08-19 E I Du Pont De Nemours And Company Electroblowing web formation process
CN201195772Y (zh) * 2008-05-15 2009-02-18 东华大学 一种带有温控装置的泡泡纺设备

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0009941A1 (en) * 1978-10-10 1980-04-16 Imperial Chemical Industries Plc Production of electrostatically spun products
EP1673493A1 (en) 2003-09-08 2006-06-28 Technicka Univerzita v Liberci A method of nanofibres production from a polymer solution using electrostatic spinning and a device for carrying out the method
WO2012006072A2 (en) * 2010-06-28 2012-01-12 Virginia Commonwealth University Air impedance electrospinning for controlled porosity

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105586644A (zh) * 2015-12-10 2016-05-18 厦门大学 一种制备蓬松纳米纤维的电纺装置
CN106637439A (zh) * 2017-01-24 2017-05-10 厦门大学 自主式多喷头气泡静电纺丝装置
CN106637439B (zh) * 2017-01-24 2018-12-25 厦门大学 自主式多喷头气泡静电纺丝装置
CN108754874A (zh) * 2018-06-22 2018-11-06 南通纺织丝绸产业技术研究院 大孔径纳米纤维膜的制备方法
CN108774809A (zh) * 2018-06-22 2018-11-09 南通纺织丝绸产业技术研究院 批量制备大孔径纳米纤维膜的方法
CN109457304A (zh) * 2018-12-06 2019-03-12 南通纺织丝绸产业技术研究院 一种制备大孔径纳米纤维的方法

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CZ2012906A3 (cs) 2013-10-16
CZ304099B6 (cs) 2013-10-16

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