EP2661495A4 - Fusion of peptidoglycan hydrolase enzymes to a protein transduction domain allows eradication of both extracellular and intracellular gram positive pathogens - Google Patents

Fusion of peptidoglycan hydrolase enzymes to a protein transduction domain allows eradication of both extracellular and intracellular gram positive pathogens

Info

Publication number
EP2661495A4
EP2661495A4 EP11854973.2A EP11854973A EP2661495A4 EP 2661495 A4 EP2661495 A4 EP 2661495A4 EP 11854973 A EP11854973 A EP 11854973A EP 2661495 A4 EP2661495 A4 EP 2661495A4
Authority
EP
European Patent Office
Prior art keywords
extracellular
fusion
gram positive
protein transduction
transduction domain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP11854973.2A
Other languages
German (de)
French (fr)
Other versions
EP2661495A1 (en
Inventor
David M Donovan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
US Department of Agriculture USDA
Original Assignee
US Department of Agriculture USDA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by US Department of Agriculture USDA filed Critical US Department of Agriculture USDA
Publication of EP2661495A1 publication Critical patent/EP2661495A1/en
Publication of EP2661495A4 publication Critical patent/EP2661495A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/13—Dipeptidases (3.4.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24075—Lysostaphin (3.4.24.75)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
EP11854973.2A 2011-01-05 2011-01-05 Fusion of peptidoglycan hydrolase enzymes to a protein transduction domain allows eradication of both extracellular and intracellular gram positive pathogens Withdrawn EP2661495A4 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2011/020230 WO2012094004A1 (en) 2011-01-05 2011-01-05 Fusion of peptidoglycan hydrolase enzymes to a protein transduction domain allows eradication of both extracellular and intracellular gram positive pathogens

Publications (2)

Publication Number Publication Date
EP2661495A1 EP2661495A1 (en) 2013-11-13
EP2661495A4 true EP2661495A4 (en) 2014-08-27

Family

ID=46457641

Family Applications (1)

Application Number Title Priority Date Filing Date
EP11854973.2A Withdrawn EP2661495A4 (en) 2011-01-05 2011-01-05 Fusion of peptidoglycan hydrolase enzymes to a protein transduction domain allows eradication of both extracellular and intracellular gram positive pathogens

Country Status (2)

Country Link
EP (1) EP2661495A4 (en)
WO (1) WO2012094004A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20230140823A1 (en) 2020-03-19 2023-05-04 Micreos Human Health B.V. A stabilized protein of interest.

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1663315B1 (en) * 2003-09-17 2011-01-05 Rodos BioTarget GmbH Targeted lipid-drug formulations for delivery of drugs to myeloid and lymphoid immune cells
US20100221235A1 (en) * 2006-02-08 2010-09-02 Diatos Compositions and Methods for Treating Lysosomal Storage Diseases

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BORYSOWSKI JAN ET AL: "Fusion to cell-penetrating peptides will enable lytic enzymes to kill intracellular bacteria", MEDICAL HYPOTHESES, EDEN PRESS, PENRITH, US, vol. 74, no. 1, 4 August 2009 (2009-08-04), pages 164 - 166, XP002587106, ISSN: 0306-9877, DOI: 10.1016/J.MEHY.2009.07.006 *
See also references of WO2012094004A1 *

Also Published As

Publication number Publication date
EP2661495A1 (en) 2013-11-13
WO2012094004A1 (en) 2012-07-12

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Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

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17P Request for examination filed

Effective date: 20130801

AK Designated contracting states

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Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

DAX Request for extension of the european patent (deleted)
A4 Supplementary search report drawn up and despatched

Effective date: 20140730

RIC1 Information provided on ipc code assigned before grant

Ipc: C12R 1/445 20060101ALI20140724BHEP

Ipc: C12N 15/52 20060101AFI20140724BHEP

Ipc: A61K 38/43 20060101ALI20140724BHEP

Ipc: C12N 15/55 20060101ALI20140724BHEP

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20150303