EP2638158A4 - Compositions, cells, kits and methods for autologous stem cell therapy - Google Patents

Compositions, cells, kits and methods for autologous stem cell therapy

Info

Publication number
EP2638158A4
EP2638158A4 EP11840155.3A EP11840155A EP2638158A4 EP 2638158 A4 EP2638158 A4 EP 2638158A4 EP 11840155 A EP11840155 A EP 11840155A EP 2638158 A4 EP2638158 A4 EP 2638158A4
Authority
EP
European Patent Office
Prior art keywords
kits
compositions
cells
methods
stem cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP11840155.3A
Other languages
German (de)
French (fr)
Other versions
EP2638158A1 (en
Inventor
Keith A Webster
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Miami
Original Assignee
University of Miami
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Miami filed Critical University of Miami
Publication of EP2638158A1 publication Critical patent/EP2638158A1/en
Publication of EP2638158A4 publication Critical patent/EP2638158A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/069—Vascular Endothelial cells
    • C12N5/0692—Stem cells; Progenitor cells; Precursor cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/113—Antisense targeting other non-coding nucleic acids, e.g. antagomirs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/65—MicroRNA
EP11840155.3A 2010-11-11 2011-11-11 Compositions, cells, kits and methods for autologous stem cell therapy Withdrawn EP2638158A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41244910P 2010-11-11 2010-11-11
PCT/US2011/060306 WO2012065024A1 (en) 2010-11-11 2011-11-11 Compositions, cells, kits and methods for autologous stem cell therapy

Publications (2)

Publication Number Publication Date
EP2638158A1 EP2638158A1 (en) 2013-09-18
EP2638158A4 true EP2638158A4 (en) 2015-07-15

Family

ID=46051307

Family Applications (1)

Application Number Title Priority Date Filing Date
EP11840155.3A Withdrawn EP2638158A4 (en) 2010-11-11 2011-11-11 Compositions, cells, kits and methods for autologous stem cell therapy

Country Status (3)

Country Link
US (1) US20130302293A1 (en)
EP (1) EP2638158A4 (en)
WO (1) WO2012065024A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9322015B2 (en) 2011-03-09 2016-04-26 The Brigham And Women's Hospital, Inc. Methods of using microRNA-26a to promote angiogenesis
US10260067B2 (en) * 2014-10-01 2019-04-16 The Brigham And Women's Hospital, Inc. Enhancing dermal wound healing by downregulating microRNA-26a
SG10201906716QA (en) * 2015-01-20 2019-08-27 Miragen Therapeutics Inc Mir-92 inhibitors and uses thereof
US20200224165A1 (en) * 2016-07-22 2020-07-16 Senlin Li Methods and compositions for rejuvenation
US11697799B2 (en) 2019-04-15 2023-07-11 Ossium Health, Inc. System and method for extraction and cryopreservation of bone marrow
WO2022020210A1 (en) 2020-07-18 2022-01-27 Ossium Health, Inc. Permeation of whole vertebral bodies with a cryoprotectant using vacuum assisted diffusion
AU2021360590A1 (en) 2020-10-14 2023-06-15 Ossium Health, Inc. Systems and methods for extraction and cryopreservation of bone marrow
EP4262831A1 (en) 2020-12-18 2023-10-25 Ossium Health, Inc. Methods of cell therapies

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2228444A1 (en) * 2009-03-09 2010-09-15 Julius-Maximilians-Universität Würzburg microRNA for diagnostic and therapeutic purposes in cardiovascular diseases

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004071464A2 (en) * 2003-02-12 2004-08-26 Johns Hopkins University School Of Medicine Diagnostic application of differentially-expressed genes in lympho-hematopoietic stem cells
JP2009519339A (en) * 2005-12-12 2009-05-14 ザ ユニバーシティ オブ ノース カロライナ アット チャペル ヒル MicroRNAs that regulate myocyte proliferation and differentiation
US20100034794A1 (en) * 2006-10-03 2010-02-11 Van Der Strate Barry W A Endothelial progenitor cell compositions and neovascularization
DE102007052114B4 (en) * 2007-10-30 2011-01-05 T2Cure Gmbh Method for modulating the function, growth or differentiation of a cell
WO2009137807A2 (en) * 2008-05-08 2009-11-12 Asuragen, Inc. Compositions and methods related to mirna modulation of neovascularization or angiogenesis

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2228444A1 (en) * 2009-03-09 2010-09-15 Julius-Maximilians-Universität Würzburg microRNA for diagnostic and therapeutic purposes in cardiovascular diseases

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
A. BONAUER ET AL: "MicroRNA-92a Controls Angiogenesis and Functional Recovery of Ischemic Tissues in Mice - Supporting Online Material", SCIENCE, vol. 324, no. 5935, 21 May 2009 (2009-05-21), pages 1710 - 1713, XP055166478, ISSN: 0036-8075, DOI: 10.1126/science.1174381 *
INGRAM D A ET AL: "Vessel wall-derived endothelial cells rapidly proliferate because they contain a complete hierarchy of endothelial progenitor cells", BLOOD, AMERICAN SOCIETY OF HEMATOLOGY, US, vol. 105, no. 7, 1 April 2005 (2005-04-01), pages 2783 - 2786, XP002351444, ISSN: 0006-4971, DOI: 10.1182/BLOOD-2004-08-3057 *
KUEHBACHER ET AL: "Targeting microRNA expression to regulate angiogenesis", TRENDS IN PHARMACOLOGICAL SCIENCES, ELSEVIER, HAYWARTH, GB, vol. 29, no. 1, 18 December 2007 (2007-12-18), pages 12 - 15, XP022410624, ISSN: 0165-6147, DOI: 10.1016/J.TIPS.2007.10.014 *
See also references of WO2012065024A1 *
URBICH C ET AL: "Role of microRNAs in vascular diseases, inflammation, and angiogenesis", CARDIOVASCULAR RESEARCH, OXFORD UNIVERSITY PRESS, GB, vol. 79, no. 4, 1 September 2008 (2008-09-01), pages 581 - 588, XP002542047, ISSN: 0008-6363, [retrieved on 20080611], DOI: 10.1093/CVR/CVN156 *

Also Published As

Publication number Publication date
EP2638158A1 (en) 2013-09-18
WO2012065024A1 (en) 2012-05-18
US20130302293A1 (en) 2013-11-14

Similar Documents

Publication Publication Date Title
IL273159A (en) Culture medium composition, and method for culturing cell or tissue using said composition
EP2545928A4 (en) Cell preparation containing mesenchymal stem cells, and method for producing same
ZA201708538B (en) Methods for purifying cells derived from pluripotent stem cells
IL214798A0 (en) Methods and compositions for stem cell cultures
ZA201202685B (en) Methods of preparing mesenchymal stem cells,compositions and kit thereof
EP2811011A4 (en) Cell culture kit, and method of using cell culture kit
EP2651207A4 (en) Methods for improving competency of plant cells
EP2637702A4 (en) Methods, compositions, cells, and kits for treating ischemic injury
EP2638158A4 (en) Compositions, cells, kits and methods for autologous stem cell therapy
EP2440652A4 (en) Methods for culturing stem and progenitor cells
EP2612911A4 (en) Composition for inducing pluripotent stem cell, and use thereof
WO2011156654A3 (en) Pathways characterization of cells
EP2670851A4 (en) Systems, tip assemblies, methods and kits for introducing material into cells
HK1205942A1 (en) Compositions, methods, and kits for regulating energy metabolism
ZA201300101B (en) Methods and compositions for enhancing stem cell mobilization
IL227529A0 (en) Compositions and methods for cell transplantation
EP2424978A4 (en) Compositions and methods for modulating stem cells and uses thereof
EP2643454A4 (en) Expanding hematopoietic stem cells
EP2700707A4 (en) Cell culture method and cell culture kit
IL227516A0 (en) Method for preserving cells and cell cultures
EP2569420A4 (en) Method and composition for inducing human pluripotent stem cells
EP2573168A4 (en) Methods for producing nerve cells from stem cells, nerve cells and uses thereof
EP2823038A4 (en) Placental stem cells, methods for isolating same and use thereof
WO2012078746A9 (en) Compositions and methods for mobilizing stem cells
EP2689022A4 (en) Compositions and methods for enhancing the pluripotency of stem cells

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20130610

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

DAX Request for extension of the european patent (deleted)
RIC1 Information provided on ipc code assigned before grant

Ipc: C07H 21/02 20060101ALI20150205BHEP

Ipc: C12N 15/11 20060101AFI20150205BHEP

Ipc: C12N 5/16 20060101ALI20150205BHEP

RA4 Supplementary search report drawn up and despatched (corrected)

Effective date: 20150612

RIC1 Information provided on ipc code assigned before grant

Ipc: C07H 21/02 20060101ALI20150608BHEP

Ipc: C12N 15/11 20060101AFI20150608BHEP

Ipc: C12N 5/16 20060101ALI20150608BHEP

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20160112