EP2512675A1 - Microfluidics apparatus and methods - Google Patents
Microfluidics apparatus and methodsInfo
- Publication number
- EP2512675A1 EP2512675A1 EP10793029A EP10793029A EP2512675A1 EP 2512675 A1 EP2512675 A1 EP 2512675A1 EP 10793029 A EP10793029 A EP 10793029A EP 10793029 A EP10793029 A EP 10793029A EP 2512675 A1 EP2512675 A1 EP 2512675A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- particles
- electrodes
- concentration
- fluid
- sensing surface
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract description 17
- 239000002245 particle Substances 0.000 claims abstract description 105
- 239000012530 fluid Substances 0.000 claims abstract description 53
- 230000005684 electric field Effects 0.000 claims abstract description 18
- 239000000758 substrate Substances 0.000 claims abstract description 9
- 238000006243 chemical reaction Methods 0.000 claims description 7
- 239000012141 concentrate Substances 0.000 claims description 7
- 229920000642 polymer Polymers 0.000 claims description 5
- 239000000427 antigen Substances 0.000 claims description 2
- 102000036639 antigens Human genes 0.000 claims 1
- 108091007433 antigens Proteins 0.000 claims 1
- 230000000694 effects Effects 0.000 description 10
- 239000011521 glass Substances 0.000 description 9
- 238000013461 design Methods 0.000 description 8
- 238000001514 detection method Methods 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 7
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 7
- 239000010931 gold Substances 0.000 description 5
- 229910052737 gold Inorganic materials 0.000 description 5
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 4
- 241000194107 Bacillus megaterium Species 0.000 description 3
- 239000004593 Epoxy Substances 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 230000035784 germination Effects 0.000 description 3
- 239000000017 hydrogel Substances 0.000 description 3
- AMGQUBHHOARCQH-UHFFFAOYSA-N indium;oxotin Chemical compound [In].[Sn]=O AMGQUBHHOARCQH-UHFFFAOYSA-N 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- 239000012620 biological material Substances 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
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- 238000005259 measurement Methods 0.000 description 2
- 229910052697 platinum Inorganic materials 0.000 description 2
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- 239000000725 suspension Substances 0.000 description 2
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- WYTGDNHDOZPMIW-RCBQFDQVSA-N alstonine Natural products C1=CC2=C3C=CC=CC3=NC2=C2N1C[C@H]1[C@H](C)OC=C(C(=O)OC)[C@H]1C2 WYTGDNHDOZPMIW-RCBQFDQVSA-N 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 230000008602 contraction Effects 0.000 description 1
- 238000004720 dielectrophoresis Methods 0.000 description 1
- 239000004205 dimethyl polysiloxane Substances 0.000 description 1
- 235000013870 dimethyl polysiloxane Nutrition 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
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- 238000002474 experimental method Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 238000002032 lab-on-a-chip Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 229920002120 photoresistant polymer Polymers 0.000 description 1
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 description 1
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 1
- 229920005597 polymer membrane Polymers 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 238000009790 rate-determining step (RDS) Methods 0.000 description 1
- 238000002310 reflectometry Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
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- 239000010936 titanium Substances 0.000 description 1
- 229910052719 titanium Inorganic materials 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0645—Electrodes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0663—Whole sensors
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
- B01L2400/0424—Dielectrophoretic forces
Abstract
Description
Claims
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB0921840.5A GB2476235B (en) | 2009-12-15 | 2009-12-15 | Microfluidics apparatus and methods |
PCT/GB2010/052074 WO2011073643A1 (en) | 2009-12-15 | 2010-12-13 | Microfluidics apparatus and methods |
Publications (2)
Publication Number | Publication Date |
---|---|
EP2512675A1 true EP2512675A1 (en) | 2012-10-24 |
EP2512675B1 EP2512675B1 (en) | 2018-07-18 |
Family
ID=41667075
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP10793029.9A Active EP2512675B1 (en) | 2009-12-15 | 2010-12-13 | Microfluidics apparatus and methods |
Country Status (6)
Country | Link |
---|---|
US (1) | US9302263B2 (en) |
EP (1) | EP2512675B1 (en) |
CN (1) | CN102753268A (en) |
GB (1) | GB2476235B (en) |
SG (1) | SG181775A1 (en) |
WO (1) | WO2011073643A1 (en) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102390103B (en) * | 2011-07-11 | 2013-10-16 | 西安交通大学 | Electric field enhanced capillary micro injection moulding method |
US10222391B2 (en) | 2011-12-07 | 2019-03-05 | The Johns Hopkins University | System and method for screening a library of samples |
CN104248998A (en) * | 2013-06-27 | 2014-12-31 | 李木 | Analog and digital combination type micro-fluidic chip and control method |
CN104965081B (en) * | 2015-05-29 | 2016-08-17 | 刘晓竹 | Antibody antigen detection method based on mobile device |
CN104965080B (en) * | 2015-05-29 | 2017-03-01 | 重庆大学 | A kind of reaction member for detection antibody or antigen and system |
CN106378216B (en) * | 2016-11-09 | 2019-05-21 | 杭州电子科技大学 | A kind of particulate sorter based on negative magnetophoresis technology |
GB2573323A (en) * | 2018-05-03 | 2019-11-06 | Mursia Ltd | Biosensor method and system |
US20230132688A1 (en) * | 2021-10-28 | 2023-05-04 | Worcester Polytechnic Institute | Gravity independent liquid cooling for electronics |
Family Cites Families (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9208357D0 (en) * | 1992-04-16 | 1992-06-03 | British Tech Group | Apparatus for separating a mixture |
US5858192A (en) * | 1996-10-18 | 1999-01-12 | Board Of Regents, The University Of Texas System | Method and apparatus for manipulation using spiral electrodes |
DE19983263T1 (en) * | 1998-05-29 | 2001-05-31 | Ind Res Ltd | Method and device for concentrating and / or positioning particles or cells |
US6352838B1 (en) | 1999-04-07 | 2002-03-05 | The Regents Of The Universtiy Of California | Microfluidic DNA sample preparation method and device |
US6942776B2 (en) * | 1999-05-18 | 2005-09-13 | Silicon Biosystems S.R.L. | Method and apparatus for the manipulation of particles by means of dielectrophoresis |
US6787018B1 (en) * | 2000-12-08 | 2004-09-07 | The Regents Of The University Of California | Dielectrophoretic concentration of particles under electrokinetic flow |
US6685812B2 (en) * | 2001-01-09 | 2004-02-03 | The Regents Of The University Of California | Movement of particles using sequentially activated dielectrophoretic particle trapping |
US6761811B2 (en) * | 2001-03-27 | 2004-07-13 | The Regents Of The University Of California | Multi-stage separations based on dielectrophoresis |
ITTO20010801A1 (en) * | 2001-08-07 | 2003-02-07 | Silicon Biosystems S R L | METHOD AND DEVICE FOR INTEGRATED BIOMOLECULAR ANALYSIS. |
US20040011650A1 (en) * | 2002-07-22 | 2004-01-22 | Frederic Zenhausern | Method and apparatus for manipulating polarizable analytes via dielectrophoresis |
CA2511331A1 (en) * | 2002-12-20 | 2004-07-15 | Board Of Regents, The University Of Texas System | Methods and apparatus for electrosmear analysis |
GB0305587D0 (en) | 2003-03-11 | 2003-04-16 | Smart Holograms Ltd | Sensor |
JP4328168B2 (en) * | 2003-10-02 | 2009-09-09 | ソニー株式会社 | Detection unit for interaction between substances using capillary phenomenon, method using the detection unit, and substrate for bioassay |
GB0517447D0 (en) * | 2005-08-25 | 2005-10-05 | Smart Holograms Ltd | Use of holographic sensor |
US7964078B2 (en) * | 2005-11-07 | 2011-06-21 | The Regents Of The University Of California | Microfluidic device for cell and particle separation |
US8182669B2 (en) * | 2005-11-18 | 2012-05-22 | The Regents Of The University Of California | Dynamic equilibrium separation, concentration, and mixing apparatus and methods |
EP1830177A1 (en) * | 2006-03-02 | 2007-09-05 | F. Hoffman-la Roche AG | Integrated test element |
WO2007107910A1 (en) * | 2006-03-21 | 2007-09-27 | Koninklijke Philips Electronics N. V. | Microelectronic device with field electrodes |
CN101405083A (en) * | 2006-03-21 | 2009-04-08 | 皇家飞利浦电子股份有限公司 | Microelectronic device with field electrodes |
GB0804491D0 (en) * | 2008-03-11 | 2008-04-16 | Iti Scotland Ltd | Detecting analytes |
US20130146459A1 (en) * | 2009-06-16 | 2013-06-13 | Massachusetts Institute Of Technology | Multiphase non-linear electrokinetic devices |
-
2009
- 2009-12-15 GB GB0921840.5A patent/GB2476235B/en active Active
-
2010
- 2010-12-13 SG SG2012044772A patent/SG181775A1/en unknown
- 2010-12-13 WO PCT/GB2010/052074 patent/WO2011073643A1/en active Application Filing
- 2010-12-13 CN CN201080063934XA patent/CN102753268A/en active Pending
- 2010-12-13 EP EP10793029.9A patent/EP2512675B1/en active Active
- 2010-12-13 US US13/516,442 patent/US9302263B2/en active Active
Non-Patent Citations (1)
Title |
---|
See references of WO2011073643A1 * |
Also Published As
Publication number | Publication date |
---|---|
US20120276550A1 (en) | 2012-11-01 |
GB0921840D0 (en) | 2010-01-27 |
SG181775A1 (en) | 2012-07-30 |
CN102753268A (en) | 2012-10-24 |
EP2512675B1 (en) | 2018-07-18 |
US9302263B2 (en) | 2016-04-05 |
GB2476235A (en) | 2011-06-22 |
WO2011073643A1 (en) | 2011-06-23 |
GB2476235B (en) | 2013-07-10 |
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